All Phenotypes Ikbkb<tm1Ver> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ikbkb^tm1Ver/Ikbkb^tm1Ver	involves: 129S4/SvJae * C57BL/6J	MGI:3609489
cx2	Chuk^tm1Ver/Chuk^tm1Ver Ikbkb^tm1Ver/Ikbkb^tm1Ver Tnfrsf1a^tm1Mak/Tnfrsf1a^tm1Mak	involves: 129/Sv * 129S4/SvJae * C57BL/6J	MGI:3609629
cx3	Ikbkb^tm1Ver/Ikbkb^tm1Ver Tnfrsf1a^tm1Mak/Tnfrsf1a^tm1Mak	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6J	MGI:3609627
cx4	Chuk^tm1Ver/Chuk^tm1Ver Ikbkb^tm1Ver/Ikbkb^tm1Ver	involves: 129S4/SvJae * C57BL/6J	MGI:3609628

Allelic Composition	Ikbkb^tm1Ver/Ikbkb^tm1Ver
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ikbkb^tm1Ver mutation (0 available); any Ikbkb mutation (54 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:54323 )

• prior to E12.5, homozygotes appear morphologically normal; however, mutant embryos die progressively from E12.5 to E14 as a result of massive liver degeneration

liver/biliary system

liver hemorrhage ( J:54323 )

• dead or moribund mutant embryos exhibit massive liver hemorrhage

increased hepatocyte apoptosis ( J:54323 )

• at E13, homozygotes exhibit extensive hepatocyte apoptosis, as shown by TUNEL assays

dissociated hepatocytes ( J:54323 )

• at E13, mutant hepatocytes appear dissociated and dying, as evidenced by the presence of pyknotic nuclei; however, fetal liver hematopoeisis appears unaffected

small liver ( J:54323 )

• at E13 and E13.5, surviving homozygotes are of normal body size and morphology but exhibit smaller and darker livers than wild-type mice

liver degeneration ( J:54323 )

• dead or moribund mutant embryos exhibit massive liver degeneration

cardiovascular system

liver hemorrhage ( J:54323 )

• dead or moribund mutant embryos exhibit massive liver hemorrhage

cellular

abnormal cell physiology ( J:54323 )

• mutant MEFs exhibit significantly reduced NF-kappaB activation upon induction with TNF or IL-1alpha

increased fibroblast apoptosis ( J:54323 )

• mutant MEFs exhibit increased sensitivity to TNF-induced apoptosis relative to wild-type or heterozygous MEFs

increased hepatocyte apoptosis ( J:54323 )

• at E13, homozygotes exhibit extensive hepatocyte apoptosis, as shown by TUNEL assays

Allelic Composition	Chuk^tm1Ver/Chuk^tm1Ver Ikbkb^tm1Ver/Ikbkb^tm1Ver Tnfrsf1a^tm1Mak/Tnfrsf1a^tm1Mak
Genetic Background	involves: 129/Sv * 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chuk^tm1Ver mutation (0 available); any Chuk mutation (48 available)
Ikbkb^tm1Ver mutation (0 available); any Ikbkb mutation (54 available)
Tnfrsf1a^tm1Mak mutation (2 available); any Tnfrsf1a mutation (47 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:63443 )

• triple homozygotes survive to ~E16.5, exhibiting a morphology similar to that observed in Chuk^tm1Ver mutant embryos

limbs/digits/tail

broad limb buds ( J:63443 )

• at E14.5, triple homozygotes exhibit dumpy limb buds, similar to those observed in Chuk^tm1Ver mutant embryos

curly tail ( J:63443 )

• at E14.5, triple homozygotes exhibit curled tails, similar to those observed in Chuk^tm1Ver mutant embryos

nervous system

open neural tube ( J:63443 )

• at E14.5, triple homozygotes exhibit a neural tube defect, similar to that observed in mice doubly homozygous for Chuk^tm1Ver and Ikbkb^tm1Ver

embryo

broad limb buds ( J:63443 )

• at E14.5, triple homozygotes exhibit dumpy limb buds, similar to those observed in Chuk^tm1Ver mutant embryos

open neural tube ( J:63443 )

• at E14.5, triple homozygotes exhibit a neural tube defect, similar to that observed in mice doubly homozygous for Chuk^tm1Ver and Ikbkb^tm1Ver

Allelic Composition	Ikbkb^tm1Ver/Ikbkb^tm1Ver Tnfrsf1a^tm1Mak/Tnfrsf1a^tm1Mak
Genetic Background	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ikbkb^tm1Ver mutation (0 available); any Ikbkb mutation (54 available)
Tnfrsf1a^tm1Mak mutation (2 available); any Tnfrsf1a mutation (47 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality ( J:54323 )

• double homozygotes develop to term but die within the first postnatal month, unlike Ikbkb^tm1Ver mutant embryos that die at ~E12.5-E13.5

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:63443 )

• double mutant embryos are obtained at the expected frequency at E11.5, but die at E12 as a result of liver dysfunction

liver/biliary system

increased hepatocyte apoptosis ( J:63443 )

• at E11.5-E12, double homozygotes exhibit a massive increase in liver apoptosis relative to wild-type embryos, indicating liver dysfunction

liver degeneration ( J:63443 )

nervous system

increased hindbrain apoptosis ( J:63443 )

• at E9.5, increased apoptosis is noted in the neuroepithelium of double mutant hindbrain

increased embryonic neuroepithelium apoptosis ( J:63443 )

• at E9.5, double homozygous mutant embryos show increased apoptosis in the neuronal epithelium at the hindbrain level

• however, no defects in neural differentiation are observed

increased spinal cord apoptosis ( J:63443 )

• at ~E11.5-E12, double homozygotes exhibit a 2-fold increase in apoptosis in the spinal cord relative to wild-type mice

open neural tube ( J:63443 )

• ~70% of double homozygotes fail to close the neural tube in the hindbrain

small embryonic telencephalon ( J:63443 )

• double mutant embryos exhibit reduced telencephalic vesicles relative to wild-type embryos

abnormal hindbrain morphology ( J:63443 )

• at E9.5, double homozygotes lack the roof of hindbrain

abnormal dorsal root ganglion morphology ( J:63443 )

• at about E11.5-E12, double homozygotes exhibit a 2-fold increase in apoptosis in dorsal root ganglia relative to wild-type mice

cellular

abnormal cell physiology ( J:63443 )

• double mutant MEFs exhibit no detectable NF-kappaB DNA binding activity upon induction with human TNF, IL-1alpha, or LPS

• notably, NF-kappaB activation is more attenuated in single Ikbkb^tm1Ver mutant MEFs than in single Chuk^tm1Ver mutant MEFs

increased hindbrain apoptosis ( J:63443 )

• at E9.5, increased apoptosis is noted in the neuroepithelium of double mutant hindbrain

increased hepatocyte apoptosis ( J:63443 )

• at E11.5-E12, double homozygotes exhibit a massive increase in liver apoptosis relative to wild-type embryos, indicating liver dysfunction

increased embryonic neuroepithelium apoptosis ( J:63443 )

• at E9.5, double homozygous mutant embryos show increased apoptosis in the neuronal epithelium at the hindbrain level

• however, no defects in neural differentiation are observed

increased spinal cord apoptosis ( J:63443 )

• at ~E11.5-E12, double homozygotes exhibit a 2-fold increase in apoptosis in the spinal cord relative to wild-type mice

embryo

abnormal neural fold elevation formation ( J:63443 )

• at E9.5, double homozygotes exhibit defective neurulation: neural folds at the hindbrain level fail to elevate on either side of the midline and do not bend toward each other

open neural tube ( J:63443 )

• ~70% of double homozygotes fail to close the neural tube in the hindbrain

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