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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Mmp2tm1Ito
targeted mutation 1, Shigeyoshi Itohara
MGI:2386252
Summary 12 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Mmp2tm1Ito/Mmp2tm1Ito B6.129P2-Mmp2tm1Ito MGI:4365601
hm2
Mmp2tm1Ito/Mmp2tm1Ito involves: 129P2/OlaHsd MGI:4367276
hm3
Mmp2tm1Ito/Mmp2tm1Ito involves: 129P2/OlaHsd * C57BL/6 MGI:3583006
hm4
Mmp2tm1Ito/Mmp2tm1Ito involves: 129P2/OlaHsd * C57BL/6J MGI:3577310
hm5
Mmp2tm1Ito/Mmp2tm1Ito Not Specified MGI:3046922
cx6
Mmp2tm1Ito/Mmp2tm1Ito
Timp1tm1Pds/Timp1tm1Pds
either: (involves: 129S4/SvJae) or (involves: C57BL/6) MGI:3525497
cx7
Mmp2tm1Ito/Mmp2tm1Ito
Mmp9tm1Tvu/Mmp9tm1Tvu
involves: 129P2/OlaHsd * 129S6/SvEvTac MGI:4367346
cx8
Mmp2tm1Ito/Mmp2tm1Ito
Timp4tm1Vuo/Timp4tm1Vuo
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 MGI:4839035
cx9
Mmp2tm1Ito/Mmp2tm1Ito
Timp3tm1Osya/Timp3tm1Osya
involves: 129P2/OlaHsd * C57BL/6 MGI:5516118
cx10
Mmp2tm1Ito/Mmp2tm1Ito
Recktm1Ito/Recktm1Ito
involves: 129P2/OlaHsd * C57BL/6 MGI:3687637
cx11
Mmp14tm1Noda/Mmp14tm1Noda
Mmp2tm1Ito/Mmp2tm1Ito
Not Specified MGI:3046924
cx12
Mmp14tm1Noda/Mmp14+
Mmp2tm1Ito/Mmp2tm1Ito
Not Specified MGI:3046923


Genotype
MGI:4365601
hm1
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Genetic
Background
B6.129P2-Mmp2tm1Ito
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
N
• mice injected with Lewis lung carcinoma cells exhibit normal tumor growth




Genotype
MGI:4367276
hm2
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• mice exhibit 35% less ischemia-induced retinal neovascularization compared with similarly treated wild-type mice

skeleton
• hyperteloric with narrower, taller skulls
• overall skull lengths are decreased and about 10% shorter by 12 weeks of age
• area between the right and left frontal-squamosal intersection at the temporal crest, mid-cranial width, is wider and this difference increases with age
• mid-cranial width differences continue to increase from about 10% at 4 weeks to nearly 25% at 24 weeks
• coronal sutures are prominent and sclerotic
• lambdoid sutures are prominent and sclerotic
• sagittal sutures are prominent and sclerotic
• lower jaw length is about 10% shorter by 12 weeks of age
• upper jaw length is about 10% shorter by 12 weeks of age
• bone marrow has transiently decreased osteoclast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
• femoral condyles and proximal tibiae show cartilage destruction with erosions of cartilage occupied by fibrous tissue and inflammatory cells
• 6 month old mutants show a slight shortening of long bones
• progressive loss of bone mineral density; mutants begin to show loss at 5 weeks of age and show a 20% reduction at 10 weeks of age that persists throughout early adulthood, and with more rapid loss after 20 weeks of age
• 13.4% decrease in bone volume at 24 weeks of age
• defects in cortical bone formation in 4 day old mutants that appear to mostly resolve by 4 weeks of age
• cortical bone continues to show large areas of woven bone compared to normal lamellar tissue in wild-type bone at 4 and 12 weeks of age
• bone marrow has transiently decreased osteoblast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
• defects in trabecular bone formation in 4 day old mutants that appear to mostly resolve by 4 weeks of age
• primary spongiosa and trabeculae appear less dense and more scant
• knee joints of all 12 week old mutants show articular cartilage destruction and erosion of the underlying bone surface resulting in the loss of the smooth tibial and femoral surfaces
• bone of 4 day old mutants shows overall paucity and chaotic organization of trabecular bone, and marked hypocellularity and ragged metaphyseal and diaphyseal cortical bone, a phenotype seen in earlier embryological time points, indicating retarded bone growth
• stigmata of abnormal cortical growth remains at 4 weeks of age

craniofacial
• hyperteloric with narrower, taller skulls
• overall skull lengths are decreased and about 10% shorter by 12 weeks of age
• area between the right and left frontal-squamosal intersection at the temporal crest, mid-cranial width, is wider and this difference increases with age
• mid-cranial width differences continue to increase from about 10% at 4 weeks to nearly 25% at 24 weeks
• coronal sutures are prominent and sclerotic
• lambdoid sutures are prominent and sclerotic
• sagittal sutures are prominent and sclerotic
• lower jaw length is about 10% shorter by 12 weeks of age
• upper jaw length is about 10% shorter by 12 weeks of age
• broad snout
• snouts are about 15% shorter than wild-type at 4 weeks of age

growth/size/body
• broad snout
• snouts are about 15% shorter than wild-type at 4 weeks of age

hematopoietic system
• bone marrow has transiently decreased osteoclast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
• 60% decrease in the number of proliferating cells in the bone marrow at 4 days of age but not at 4 or 12 weeks of age
• bone marrow stromal cells and calvaria are unable to support osteoblast and osteoclast growth ex vivo

immune system
• bone marrow has transiently decreased osteoclast numbers
• osteoblast and osteoclast numbers per trabecular bone surface area are decreased by more than half at 4 days of age, but no differences are seen at 4 and 12 weeks of age, indicating a transient decrease
• femoral condyles and proximal tibiae show cartilage destruction with erosions of cartilage occupied by fibrous tissue and inflammatory cells

vision/eye
• mice exhibit 35% less ischemia-induced retinal neovascularization compared with similarly treated wild-type mice
• intercanthal distances are increased by about 10% at 4 weeks of age, however by 12 weeks of age, they are no longer statistically different




Genotype
MGI:3583006
hm3
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• delayed alveolar formation, however lungs were indistinguishable from wild-type by P14




Genotype
MGI:3577310
hm4
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• reduced body size, even at birth
• growth rate about 15% slower than normal

cardiovascular system
• exhibit significantly reduced carotid artery intimal hyperplasia in response to vascular injury and fewer intimal smooth muscle cells
• reduced neovascularization of implanted melanoma cells (J:46091)
• thickness of choroidal neovascular membranes was 31% lower than normal after treatment with krypton lasers (J:81029)
• left ventricular diameters did not increase as much after infarction as they did in controls
• better survival in the first 7 days after myocardial infarction than controls (90% survival as compared to 61%)
• exhibit impairment of smooth muscle cell migration through a gelatin-coated membrane towards a chemoattractant and in a wound assay

immune system
• earlier onset and increased severity of experimental autoimmune encephalomyelitis induced by immunization with myelin oligodendrocyte glycoprotein
• inflammatory cell infiltration and granulation tissue seen in response to implanted melanoma cells but to a lesser extent than in controls
• increased T-cell transmigration through the brain endothelium, dependent on the increased expression of Mmp9 seen in lymphocytes

neoplasm
• reduced tumor development after implantation of melanoma cells

nervous system
• increased T-cell transmigration through the brain endothelium, dependent on the increased expression of Mmp9 seen in lymphocytes

muscle
• exhibit impairment of smooth muscle cell migration through a gelatin-coated membrane towards a chemoattractant and in a wound assay

homeostasis/metabolism
• better survival in the first 7 days after myocardial infarction than controls (90% survival as compared to 61%)




Genotype
MGI:3046922
hm5
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• homozygous mutants are indistinguishable from wild-type littermates




Genotype
MGI:3525497
cx6
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Timp1tm1Pds/Timp1tm1Pds
Genetic
Background
either: (involves: 129S4/SvJae) or (involves: C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
Timp1tm1Pds mutation (1 available); any Timp1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• resistance to corneal infection is similar to Timp1 single homozygotes




Genotype
MGI:4367346
cx7
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Mmp9tm1Tvu/Mmp9tm1Tvu
Genetic
Background
involves: 129P2/OlaHsd * 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
Mmp9tm1Tvu mutation (2 available); any Mmp9 mutation (49 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• following laser trauma, mice exhibit decreased choroidal neovascularization compared with similarly treated single homozygote

vision/eye
• following laser trauma, mice exhibit decreased choroidal neovascularization compared with similarly treated single homozygote




Genotype
MGI:4839035
cx8
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Timp4tm1Vuo/Timp4tm1Vuo
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
Timp4tm1Vuo mutation (0 available); any Timp4 mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Cardiac neutrophil accumulation post-myocardial infraction is normalized in Timp4tm1Vuo/Timp4tm1Vuo Mmp2tm1Ito/Mmp2tm1Ito mice

cardiovascular system
N
• mice exhibit normal mortality and neutrophil accumulation following left anterior descending coronary artery ligation




Genotype
MGI:5516118
cx9
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Timp3tm1Osya/Timp3tm1Osya
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
Timp3tm1Osya mutation (0 available); any Timp3 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• angiotensin II infused mutants exhibit a greater disruption of the medial elastic lamellae and fibrillar structures in the abdominal aortic walls than in single Timp3 homozygotes
• angiotensin II infused mutants exhibit a greater disruption of the medial elastic lamellae and fibrillar structures in the abdominal aortic walls than in single Timp3 homozygotes
• 75% of mutants develop abdominal aortic aneurysm with greater than 50% aortic dilation in the suprarenal region after 4 weeks of angiotensin II infusion
• survival is compromised more than in single Timp3 homozygotes after angiotensin II infusion due to aortic rupture, with mice showing around 70% survival 28 days after infusion
• treatment with a broad spectrum protease inhibitor, PD166793, during the course of angiotensin II infusion blocks abdominal aortic aneurysm development
• mutants treated with angiotensin II exhibit heightened inflammation in the abdominal aorta, with enhanced infiltration of neutrophils and macrophages

immune system
• mutants treated with angiotensin II exhibit heightened inflammation in the abdominal aorta, with enhanced infiltration of neutrophils and macrophages

mortality/aging
• survival is compromised more than in single Timp3 homozygotes after angiotensin II infusion due to aortic rupture, with mice showing around 70% survival 28 days after infusion




Genotype
MGI:3687637
cx10
Allelic
Composition
Mmp2tm1Ito/Mmp2tm1Ito
Recktm1Ito/Recktm1Ito
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
Recktm1Ito mutation (1 available); any Reck mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• 2/3 die by E11 and none survive beyond E11.5, however exhibit partial rescue of the phenotypes seen in single Reck homozygotes such as increased body size and tissue integrity




Genotype
MGI:3046924
cx11
Allelic
Composition
Mmp14tm1Noda/Mmp14tm1Noda
Mmp2tm1Ito/Mmp2tm1Ito
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp14tm1Noda mutation (0 available); any Mmp14 mutation (43 available)
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• neonates die within 10 minutes of birth

cardiovascular system
• the proportion of vessels with a diameter greater than 30 nm is reduced
• many capillaries with minimal luminal opening are seen

growth/size/body
• neonates weigh about 10 - 30% less than Mmp2tm1Ito single homozygotes

homeostasis/metabolism
• neonates appear cyanotic and have difficulty breathing

muscle
• more muscle fibers appear to be immature with central nuclei present and in vitro myotube formation is impaired
• the back and intercostal muscles have shorter muscle fibers in an abnormal wavy pattern
• the diaphragm is consistently thinner in double mutants compared to Mmp2tm1Ito single homozygotes

skeleton
• the skull and bones are generally smaller
• ossification and growth plate vascularization are impaired




Genotype
MGI:3046923
cx12
Allelic
Composition
Mmp14tm1Noda/Mmp14+
Mmp2tm1Ito/Mmp2tm1Ito
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mmp14tm1Noda mutation (0 available); any Mmp14 mutation (43 available)
Mmp2tm1Ito mutation (1 available); any Mmp2 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• mutants weigh about 20% less than control littermates but are fertile





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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory