Phenotypes associated with this allele

• Allele Symbol: Plcg2^tm1Jni
• Allele Name: targeted mutation 1, James N Ihle
• Allele ID: MGI:2386100
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plcg2^tm1Jni/Plcg2^tm1Jni	involves: 129P2/OlaHsd	MGI:3796413
hm2	Plcg2^tm1Jni/Plcg2^tm1Jni	involves: 129P2/OlaHsd * C57BL/6	MGI:4940196
cx3	Pik3r1^tm1Dfr/Pik3r1^tm1Dfr Plcg2^tm1Jni/Plcg2^tm1Jni	involves: 129P2/OlaHsd * 129S6/SvEvTac	MGI:4940201
cx4	Plcg2^tm1Jni/Plcg2^tm1Jni Tg(IghMyc)22Bri/0	involves: 129P2/OlaHsd * C57BL * C57BL/6 * SJL	MGI:4940206

Genotype
MGI:3796413

hm1

• Allelic Composition: Plcg2^tm1Jni/Plcg2^tm1Jni
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Plcg2^tm1Jni/Plcg2^tm1Jni mice are protected from mBSA-induced arthritis

immune system

increased transitional stage T2 B cell number ( J:86373 )

• slight

increased pro-B cell number ( J:104166 )

• slight in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

arrested B cell differentiation ( J:86373 )

• at the T2 to follicular B cell transition

decreased mature B cell number ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

decreased follicular B cell number ( J:86373 )

abnormal dendritic cell morphology ( J:157619 )

• dendritic cells appear larger and rounder than wild-type cells

• dendritic cells exhibit abnormal actin organization compared with wild-type mice

abnormal B cell physiology ( J:80614 )

• stimulated B cells exhibit no increase in intracellular calcium unlike in wild-type cells

increased B cell apoptosis ( J:86373 )

• BCR-mediated, especially in T2 and follicular B cells

decreased B cell proliferation ( J:80614 )

abnormal dendritic cell physiology ( J:157619 )

• dendritic cells are less efficient than wild-type cells at inducing T cell proliferation

• dendritic cells restimulated ex-vivo with mBSA fail to release TH cytokines unlike similarly treated wild-type mice

abnormal dendritic cell chemotaxis ( J:157619 )

• dendritic cells exhibit impaired dendritic cell migration compared with wild-type cells

• dendritic cell homing to the lymph nodes is impaired compared to in wild-type mice

decreased susceptibility to induced arthritis ( J:157619 )

• mice are protected from mBSA-induced arthritis due to defective osteoclast recruitment and T cell activation compared with similarly treated wild-type mice

• however, wild-type dendritic cells can rescue mBSA-induced inflammation but not focal osteolysis

homeostasis/metabolism

decreased platelet aggregation ( J:114266 )

• platelets fail to form a thrombus when blood is perfused over a collagen surface at high shear speeds unlike wild-type platelets

• in a superficial lesion, mice exhibit a decreased tendency to thrombosis compared with wild-type mice

• however, mice exhibit thrombosis upon deeper subendothelial lesion

skeleton

decreased susceptibility to induced arthritis ( J:157619 )

• mice are protected from mBSA-induced arthritis due to defective osteoclast recruitment and T cell activation compared with similarly treated wild-type mice

• however, wild-type dendritic cells can rescue mBSA-induced inflammation but not focal osteolysis

hematopoietic system

abnormal dendritic cell chemotaxis ( J:157619 )

• dendritic cells exhibit impaired dendritic cell migration compared with wild-type cells

• dendritic cell homing to the lymph nodes is impaired compared to in wild-type mice

increased transitional stage T2 B cell number ( J:86373 )

• slight

increased pro-B cell number ( J:104166 )

• slight in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

arrested B cell differentiation ( J:86373 )

• at the T2 to follicular B cell transition

decreased mature B cell number ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

decreased follicular B cell number ( J:86373 )

abnormal dendritic cell morphology ( J:157619 )

• dendritic cells appear larger and rounder than wild-type cells

• dendritic cells exhibit abnormal actin organization compared with wild-type mice

abnormal B cell physiology ( J:80614 )

• stimulated B cells exhibit no increase in intracellular calcium unlike in wild-type cells

increased B cell apoptosis ( J:86373 )

• BCR-mediated, especially in T2 and follicular B cells

decreased B cell proliferation ( J:80614 )

decreased platelet aggregation ( J:114266 )

• platelets fail to form a thrombus when blood is perfused over a collagen surface at high shear speeds unlike wild-type platelets

• in a superficial lesion, mice exhibit a decreased tendency to thrombosis compared with wild-type mice

• however, mice exhibit thrombosis upon deeper subendothelial lesion

cellular

increased B cell apoptosis ( J:86373 )

• BCR-mediated, especially in T2 and follicular B cells

abnormal dendritic cell chemotaxis ( J:157619 )

• dendritic cells exhibit impaired dendritic cell migration compared with wild-type cells

• dendritic cell homing to the lymph nodes is impaired compared to in wild-type mice

decreased B cell proliferation ( J:80614 )

Genotype
MGI:4940196

hm2

• Allelic Composition: Plcg2^tm1Jni/Plcg2^tm1Jni
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal osteoclast differentiation ( J:114607 )

• in vitro and in vivo osteoclast differentiation is disrupted compared with wild-type controls

• impaired osteoclast differentiation cannot be corrected by TNF-alpha treatment

abnormal B cell differentiation ( J:117023 )

• B cell development is impaired and results in increased numbers of large pre-B cells compared to in wild-type mice

increased pre-B cell number ( J:117023 )

• in large (or early) pre-B cells

decreased osteoclast cell number ( J:114607 )

• per bone perimeter

abnormal B cell physiology ( J:117023 )

• pre-BCR-mediated functions are impaired compared to in wild-type mice

increased B cell proliferation ( J:117023 )

• 3- to 4-fold in response to IL7

• mostly in early pro-B cells and to a lesser extent in early pre-B cells

impaired natural killer cell mediated cytotoxicity ( J:100716 )

• NK cells exhibit impaired Fcgr3-, DAP12-, and DAP10-mediated cytotoxicity compared with wild-type cells

• however, target cell adhesion is normal

decreased interferon-gamma secretion ( J:100716 )

• in NK cells stimulated with YAC-1 cells or NK1.1, Ly49D, and NKG2D receptor activators

• however, IFN-gamma production stimulated by IL12 and IL18 is normal

increased susceptibility to Herpesvirales infection ( J:100716 )

• murine cytomegalovirus-infected mice exhibit 100-fold higher spleen titers compared with similarly treated wild-type mice

hematopoietic system

abnormal osteoclast differentiation ( J:114607 )

• in vitro and in vivo osteoclast differentiation is disrupted compared with wild-type controls

• impaired osteoclast differentiation cannot be corrected by TNF-alpha treatment

abnormal B cell differentiation ( J:117023 )

• B cell development is impaired and results in increased numbers of large pre-B cells compared to in wild-type mice

increased pre-B cell number ( J:117023 )

• in large (or early) pre-B cells

decreased osteoclast cell number ( J:114607 )

• per bone perimeter

abnormal B cell physiology ( J:117023 )

• pre-BCR-mediated functions are impaired compared to in wild-type mice

increased B cell proliferation ( J:117023 )

• 3- to 4-fold in response to IL7

• mostly in early pro-B cells and to a lesser extent in early pre-B cells

impaired natural killer cell mediated cytotoxicity ( J:100716 )

• NK cells exhibit impaired Fcgr3-, DAP12-, and DAP10-mediated cytotoxicity compared with wild-type cells

• however, target cell adhesion is normal

skeleton

abnormal osteoclast differentiation ( J:114607 )

• in vitro and in vivo osteoclast differentiation is disrupted compared with wild-type controls

• impaired osteoclast differentiation cannot be corrected by TNF-alpha treatment

decreased osteoclast cell number ( J:114607 )

• per bone perimeter

abnormal trabecular bone morphology ( J:114607 )

• the number of trabeculae is increased while the space between trabeculae is decreased compared to in wild-type mice

increased trabecular bone volume ( J:114607 )

increased trabecular bone thickness ( J:114607 )

cellular

abnormal osteoclast differentiation ( J:114607 )

• in vitro and in vivo osteoclast differentiation is disrupted compared with wild-type controls

• impaired osteoclast differentiation cannot be corrected by TNF-alpha treatment

increased B cell proliferation ( J:117023 )

• 3- to 4-fold in response to IL7

• mostly in early pro-B cells and to a lesser extent in early pre-B cells

Genotype
MGI:4940201

cx3

• Allelic Composition: Pik3r1^tm1Dfr/Pik3r1^tm1Dfr; Plcg2^tm1Jni/Plcg2^tm1Jni
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:104166 )

• mice die between E16 and E17 with the exception of one mouse which was runted and died within 1 day of birth

immune system

immune system phenotype ( J:104166 )

N • T cell development is normal in fetal liver transplantation assays into irradiated Jak3^tm1Jni homozygotes

increased B cell apoptosis ( J:104166 )

• in the bone marrow following fetal liver transplant into irradiated Jak3^tm1Jni homozygotes

abnormal B cell differentiation ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes, the pro- to pre-B cell transition is impeded compared to in control mice

decreased immature B cell number ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

arrested B cell differentiation ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes, peripheral B cell development is blocked compared to in control mice

decreased mature B cell number ( J:104166 )

• dramatic in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

decreased pre-B cell number ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

growth/size/body

decreased body size ( J:104166 )

• single mouse born

hematopoietic system

hematopoietic system phenotype ( J:104166 )

N • hematopoiesis is normal

increased B cell apoptosis ( J:104166 )

• in the bone marrow following fetal liver transplant into irradiated Jak3^tm1Jni homozygotes

abnormal B cell differentiation ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes, the pro- to pre-B cell transition is impeded compared to in control mice

decreased immature B cell number ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

arrested B cell differentiation ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes, peripheral B cell development is blocked compared to in control mice

decreased mature B cell number ( J:104166 )

• dramatic in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

decreased pre-B cell number ( J:104166 )

• in fetal liver transplant experiments into irradiated Jak3^tm1Jni homozygotes

cellular

increased B cell apoptosis ( J:104166 )

• in the bone marrow following fetal liver transplant into irradiated Jak3^tm1Jni homozygotes

Genotype
MGI:4940206

cx4

• Allelic Composition: Plcg2^tm1Jni/Plcg2^tm1Jni; Tg(IghMyc)22Bri/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL * C57BL/6 * SJL

mortality/aging

premature death ( J:117023 )

• mean mortality is 10 weeks

immune system

enlarged lymph nodes ( J:117023 )

• by 8 weeks

neoplasm

increased lymphoma incidence ( J:117023 )

• development of lymphoma is accelerated compared to in Tg(IghMyc)22Bri mice

increased B cell derived lymphoma incidence ( J:117023 )

• lymphomas are composed of large pre-B cells unlike in Tg(IghMyc)22Bri mice