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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Nrastm1Rak
targeted mutation 1, Raju Kucherlapati
MGI:2385944
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Nrastm1Rak/Nrastm1Rak involves: 129P2/OlaHsd * C57BL/6 MGI:3036349
cx2
Hrastm1Esn/Hrastm1Esn
Nrastm1Rak/Nrastm1Rak
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * C57BL/6N MGI:3036364
cx3
Krastm1Tyj/Krastm1Tyj
Nrastm1Rak/Nras+
involves: 129/Sv * C57BL/6 MGI:3589358
cx4
Krastm1Tyj/Kras+
Nrastm1Rak/Nrastm1Rak
involves: 129/Sv * C57BL/6 MGI:3589202
cx5
Krastm1Tyj/Krastm1Tyj
Nrastm1Rak/Nrastm1Rak
involves: 129/Sv * C57BL/6 MGI:3589289


Genotype
MGI:3036349
hm1
Allelic
Composition
Nrastm1Rak/Nrastm1Rak
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nrastm1Rak mutation (1 available); any Nras mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• viable and fertile; no gross morphological or histological abnormalities, or defects in peripheral blood cell populations were detected in homozygous mice




Genotype
MGI:3036364
cx2
Allelic
Composition
Hrastm1Esn/Hrastm1Esn
Nrastm1Rak/Nrastm1Rak
Genetic
Background
involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hrastm1Esn mutation (1 available); any Hras mutation (26 available)
Nrastm1Rak mutation (1 available); any Nras mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice grew normally, were fertile and did not exhibit any obvious phenotype; however, fewer than the expected number of adult compound homozygous mice were obtained in crosses




Genotype
MGI:3589358
cx3
Allelic
Composition
Krastm1Tyj/Krastm1Tyj
Nrastm1Rak/Nras+
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm1Tyj mutation (1 available); any Kras mutation (36 available)
Nrastm1Rak mutation (1 available); any Nras mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• ~40% of mutant embryos are found dead at E9.5




Genotype
MGI:3589202
cx4
Allelic
Composition
Krastm1Tyj/Kras+
Nrastm1Rak/Nrastm1Rak
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm1Tyj mutation (1 available); any Kras mutation (36 available)
Nrastm1Rak mutation (1 available); any Nras mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• ~30% of these mutants die perinatally
• a few mutant embryos survive up to P2, but are subsequently neglected by their mothers and die shortly thereafter
• ~70% of these mutants die between E10.0 and E12.0

cardiovascular system
• at E10.5, mutant embryos display dilated pericardial sacs
• at E10.5, mutant embryos exhibit a dilated heart

embryo
• by E10.5, cell death extends throughout the entire embryo
• at E10.5, mutant embryos appear to have arrested at ~E9.5
• ~30% of abnormal mutant embryos survive beyond this early developmental block but are readily identifiable up until the final stages of gestation
• by E10.5, 65% of viable mutant embryos appear grossly abnormal
• starting at E9.5, ~50% of mutants are delayed by 0.5-1.0 developmental days, but never show the non-coordinate delay observed in late Krastm1Tyj embryos
• at E10.5, mutant embryos display a near or complete absence of blood islands in yolk sacs
• at E10.5, mutant yolk sacs appear rough and wrinkled
• at E10.5, mutant embryos exhibit significantly reduced numbers of circulating primitive erythrocytes in either the yolk sac or the embryo

homeostasis/metabolism
• at E15.5, viable mutant embryos are developmentally delayed and severely edematous

cellular
• at E9.5, mutant embryos display prominent cell death in the forebrain, and to a lesser extent along the neural axis
• by E10.5, cell death extends throughout the entire embryo

hematopoietic system
• at E10.5, mutant embryos exhibit significantly reduced numbers of circulating primitive erythrocytes in either the yolk sac or the embryo
• at E15.5-16.5, mutant fetal livers display a significantly reduced ratio in the number of erythroblasts to hepatocytes
• at E15.5, viable mutant embryos are developmentally delayed and severely anemic
• anemia appears to be attributable to inadequate, but apparently normal, production of definitive erythrocytes and may reflect a defect in the survival or differentiation of either the erythroblasts or a more primitive progenitor cells

vision/eye
• 22% of E13.5-18.5 mutant embryos display an asymmetrical pattern in eye development, involving only the right eye
• either the right eye is significantly smaller than its wild-type counterpart or the pigment of the eye is overgrown

limbs/digits/tail
• 20% of E13.5-18.5 mutant embryos exhibit severe shortening of the tail

liver/biliary system
• by 16.5, a high % of mutant hepatocytes appear extremely vacuolated in the absence of increased cell death

growth/size/body
• by E10.5, 65% of viable mutant embryos appear grossly abnormal
• starting at E9.5, ~50% of mutants are delayed by 0.5-1.0 developmental days, but never show the non-coordinate delay observed in late Krastm1Tyj embryos

integument
• at E10.5, mutant embryos are significantly paler than wild-type embryos




Genotype
MGI:3589289
cx5
Allelic
Composition
Krastm1Tyj/Krastm1Tyj
Nrastm1Rak/Nrastm1Rak
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm1Tyj mutation (1 available); any Kras mutation (36 available)
Nrastm1Rak mutation (1 available); any Nras mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• double homozygous mutant blastocyst stage embryos are recovered at approximately the expected frequency of 6%
• no viable double mutant embryos are recovered at E9.5





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/31/2020
MGI 6.15
The Jackson Laboratory