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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tsg101tm1Mak
targeted mutation 1, Tak W Mak
MGI:2385839
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Tsg101tm1Mak/Tsg101tm1Mak either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1) MGI:2450865
cx2
Trp53tm1Tyj/Trp53tm1Tyj
Tsg101tm1Mak/Tsg101tm1Mak
involves: 129P3/J * 129S2/SvPas * C57BL/6 * CD-1 MGI:2450866


Genotype
MGI:2450865
hm1
Allelic
Composition
Tsg101tm1Mak/Tsg101tm1Mak
Genetic
Background
either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tsg101tm1Mak mutation (0 available); any Tsg101 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mutant embryos fail to develop past E6.5
• by E8.5, most embryos are either in resorption or degenerating within the yolk sac

embryo
• in vitro, E3.5 mutant blastocysts fail to show ICM proliferation after 6 days in culture
• mutant embryos fail to progress significantly beyond E5.5 due to a dramatic accumulation of Trp53 protein
• at E5.5 and esp. at E6.5, mutant embryos are significantly smaller than wild-type embryos
• at E6.5 , mutant embryos show absence of a developing mesoderm
• at E6.5, mutant embryos show absence of a primitive streak
• at E6.5, homozygotes exhibit a poorly defined boundary between the embryonic and extraembryonic regions
• at E6.5, mutant embryos display a poorly defined parietal endoderm
• at E5.5, mutant embryos display a poorly defined extraembryonic region
• by E6.5, the extraembryonic region appears severely underdeveloped and disorganized
• at E5.5, mutant embryos display a disorganized visceral endoderm

growth/size/body
• at E5.5 and esp. at E6.5, mutant embryos are significantly smaller than wild-type embryos

cellular
• at E6.5, mutant embryos exhibit a significantly lower mitotic index than wild-type embryos (0.2-0.3 versus 0.7-0.8, respectively)
• in vitro, E3.5 mutant blastocysts fail to show ICM proliferation after 6 days in culture
• at E6.5, mutant embryos display a significantly reduced cellular proliferation relative to wild-type embryos, as shown by a >40% decrease in BrdU-positive nuclei




Genotype
MGI:2450866
cx2
Allelic
Composition
Trp53tm1Tyj/Trp53tm1Tyj
Tsg101tm1Mak/Tsg101tm1Mak
Genetic
Background
involves: 129P3/J * 129S2/SvPas * C57BL/6 * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Trp53tm1Tyj mutation (9 available); any Trp53 mutation (157 available)
Tsg101tm1Mak mutation (0 available); any Tsg101 mutation (22 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• die between E8.5 and E10.5

embryo
• mutants are smaller than wild-type mice but larger than Tsg101tm1Mak homozygous mutant mice at E8.5

growth/size/body
• mutants are smaller than wild-type mice but larger than Tsg101tm1Mak homozygous mutant mice at E8.5





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last database update
12/05/2017
MGI 6.11
The Jackson Laboratory