• double transgenics show slower onset of diabetes compared to NOD-Tg(Ins2-Fasl)24Ach single transgenics

• female mice do not develop spontaneous diabetes assessed by monitoring urine glucose levels observed for >30 weeks

• 6/6 mice show accelerated diabetes development determined by urine glucose level monitoring, showing the disease by ~14 days of transfer of splenocytes from diabetic NOD mice

• when mice are injected with islet-specific CD8+ T cells, 0/5 transgenic Fas-deficient mice become diabetic whereas 5/5 transgenic Fas-sufficient mice develop diabetes 5 days after transfer

• majority of mice develop diabetes by 10 weeks of age vs controls which develop diabetes by 12 weeks of age; diabetes determined as blood glucose greater than 250 mg/dl (J:78679)

• mice develop diabetes in ~ 5 days of transfer of diabetogenic T cells (J:81416)

• at 25 weeks of age, transgenic mice show significant islet damage with little remaining insulin