Phenotypes associated with this allele

• Allele Symbol: Pla2g4a^tm1Tksh
• Allele Name: targeted mutation 1, Takao Shimizu
• Allele ID: MGI:2385582
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pla2g4a^tm1Tksh/Pla2g4a^tm1Tksh	B6.129P2-Pla2g4a^tm1Tksh	MGI:3621912
hm2	Pla2g4a^tm1Tksh/Pla2g4a^tm1Tksh	involves: 129P2/OlaHsd * C57BL/6J	MGI:3621910
hm3	Pla2g4a^tm1Tksh/Pla2g4a^tm1Tksh	NOD.129P2(B6)-Pla2g4a^tm1Tksh	MGI:3621911

Genotype
MGI:3621912

hm1

• Allelic Composition: Pla2g4a^tm1Tksh/Pla2g4a^tm1Tksh
• Genetic Background: B6.129P2-Pla2g4a^tm1Tksh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:197334 )

N • in a model of IgE antigen-dependent passive cutaneous anaphylaxis, mice exhibit normal dye extravasation (a measure of edema) and histamine release from mast cells

abnormal cytokine level ( J:107430 )

• altered levels of Th1 cytokines

decreased interferon-gamma secretion ( J:107430 )

• reduced levels of IFN gamma produced by spleen cells

decreased tumor necrosis factor secretion ( J:107430 )

• reduced levels of TNF produced by spleen cells

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:107430 )

• mice induced with myelin oligodendrocyte glycoprotein display none of the inflammatory reactions seen in controls

• symptoms are mild and infiltrates primarily restricted to mononuclear cells

homeostasis/metabolism

abnormal cytokine level ( J:107430 )

• altered levels of Th1 cytokines

Genotype
MGI:3621910

hm2

• Allelic Composition: Pla2g4a^tm1Tksh/Pla2g4a^tm1Tksh
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

growth/size/body

growth/size/body region phenotype ( J:39254 )

N • growth and development normal to 10 months of age

renal/urinary system

abnormal renal tubule morphology ( J:39254 )

• kidneys of 3 out of 5 mice have moderate cytoplasmic vacuolation in the tubular epithelium although there are no indications of abnormal kidney function

reproductive system

parturition failure ( J:39254 )

♀ • females fail to undergo labor at term, offspring born alive do not survive

♀ • if labor is induced using a progesterone receptor antagonist, 3/4 of pups born survive

decreased litter size ( J:39254 )

• homozygous females produce smaller sized litters

• regardless of the paternal genotype

respiratory system

decreased airway resistance ( J:39254 )

• lung resistance induced during anaphylaxis is slightly less than in controls and recovery is much more rapid

• bronchial hyperreactivity induced by anaphylaxis is much reduced, similar to mice unchallenged by antigen

immune system

increased susceptibility to type I hypersensitivity reaction ( J:39254 )

• reduced allergic reaction in experimentally induced anaphylaxis

Genotype
MGI:3621911

hm3

• Allelic Composition: Pla2g4a^tm1Tksh/Pla2g4a^tm1Tksh
• Genetic Background: NOD.129P2(B6)-Pla2g4a^tm1Tksh

immune system

insulitis ( J:99775 )

• mean insulitis scores increased over controls at 18 weeks of age but not at 10 weeks

abnormal macrophage physiology ( J:99775 )

• reduced production of PGE2 and increased TNF alpha production by stimulated peritoneal macrophage at 18 weeks of age

homeostasis/metabolism

impaired glucose tolerance ( J:99775 )

• increased incidence of diabetes

endocrine/exocrine glands

insulitis ( J:99775 )

• mean insulitis scores increased over controls at 18 weeks of age but not at 10 weeks

hematopoietic system

abnormal macrophage physiology ( J:99775 )

• reduced production of PGE2 and increased TNF alpha production by stimulated peritoneal macrophage at 18 weeks of age