All Phenotypes Tg(IghelMD4)4Ccg MGI Mouse

About Help FAQ

Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(IghelMD4)4Ccg
transgene insertion 4, Christopher C Goodnow
MGI:2384162

Summary

40 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cd19^tm1(cre)Cgn/Cd19⁺ Pten^tm1Hwu/Pten^tm1Hwu Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:5007685
cn2	Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(IghelMD4)4Ccg/0 Cd79a^tm1(cre)Reth/Cd79a⁺	involves: BALB/c	MGI:3797394
cn3	Cd79a^tm1(cre)Reth/Cd79a⁺ Phf5a^tm1.1Oux/Phf5a^tm1.1Oux Tg(IghelMD4)4Ccg/0	involves: BALB/c * C57BL/6 * C57BL/6J	MGI:7611733
cx4	Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0	C57BL/6-Tg(IghelMD4)4Ccg Tg(KLK4mHEL)6Ccg	MGI:3793443
cx5	Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	C57BL/6-Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg	MGI:3793461
cx6	Tlr4^Lps-d/Tlr4^Lps-d Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0	involves: 129P2/OlaHsd * C3H/HeJ * C57BL/6	MGI:3694809
cx7	Lyn^tm1Ard/Lyn⁺ Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852087
cx8	Tlr9^tm1Aki/Tlr9^tm1Aki Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3694807
cx9	Myd88^tm1Aki/Myd88^tm1Aki Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3694808
cx10	Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3841844
cx11	Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3841845
cx12	Lyn^tm1Ard/Lyn^tm1Ard Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852084
cx13	Lyn^tm1Ard/Lyn⁺ Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852085
cx14	Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852086
cx15	Cd22^tm1Eac/Cd22⁺ Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852088
cx16	Cd22^tm1Eac/Cd22⁺ Lyn^tm1Ard/Lyn⁺ Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852089
cx17	Cd22^tm1Eac/Cd22⁺ Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852090
cx18	Cd22^tm1Eac/Cd22⁺ Lyn^tm1Ard/Lyn⁺ Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:3852091
cx19	Cd19^tm1(cre)Cgn/Cd19^tm1(cre)Cgn Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:5007683
cx20	Cd19^tm1(cre)Cgn/Cd19^tm1(cre)Cgn Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:5007684
cx21	Card19^tm1Rwen/Card19^tm1Rwen Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6	MGI:7514984
cx22	Card19^tm1Rwen/Card19^tm1Rwen Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6JSfd	MGI:7514963
cx23	Fnip1^{Gt(RRM154)Byg}/Fnip1^{Gt(RRM154)Byg} Tg(IghelMD4)4Ccg/0	involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL	MGI:5445166
cx24	Cd79b^tm1Mnz/Cd79b^tm1Mnz Tg(IghelMD4)4Ccg/0 Tg(Rag2-Igb)1Mnz/0	involves: 129S1/Sv * C57BL/6	MGI:4453800
cx25	Cd79b^tm1Mnz/Cd79b^tm1Mnz Tg(BCL2)22Wehi/0 Tg(IghelMD4)4Ccg/0 Tg(Rag2-Igb)1Mnz/0	involves: 129S1/Sv * C57BL/6 * C57BL/6JWehi * SJL/JWehi	MGI:4453802
cx26	Endou^tm1Tft/Endou^tm1Tft Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	involves: 129S/SvEv * C57BL/6 * C57BL/6JSfd	MGI:5582909
cx27	Cr2^{tm2.1(HLA-A)Crr}/Cr2^{tm2.1(HLA-A)Crr} Tg(IghelMD4)4Ccg/?	involves: C57BL/6	MGI:4356056
cx28	Tg(IghelMD4)4Ccg/? Tg(KLK4mHEL)6Ccg/?	involves: C57BL/6	MGI:3799746
cx29	Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	involves: C57BL/6	MGI:3799370
cx30	Atp11c^ambr/Y Tg(IghelMD4)4Ccg/0	involves: C57BL/6 * C57BL/6JApb	MGI:5006965
cx31	Fnip1^tm1.2Baba/Fnip1^tm1.2Baba Tg(IghelMD4)4Ccg/0	involves: C57BL/6 * C57BL/6J * FVB/N * SJL	MGI:5445165
cx32	Bcl6^tm1.1Toka/Bcl6^tm1.1Toka Tg(IghelMD4)4Ccg/0	involves: C57BL/6 * NZB	MGI:5140809
cx33	Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0	NOD.B6-Tg(IghelMD4)4Ccg Tg(KLK4mHEL)6Ccg/Dvs	MGI:3793442
cx34	Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0	NOD.B6-Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg/Dvs	MGI:3793444
cx35	Ighm^tm1Cgn/Ighm^tm1Cgn Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg	NOD.Cg-Ighm^tm1Cgn Tg(IghelMD4)4Ccg/DvsJ	MGI:3793299
tg36	Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg	involves: C57BL/6	MGI:5006966
tg37	Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg	NOD.B6-Tg(IghelMD4)4Ccg/DvsJ	MGI:3793301
tg38	Tg(IghelMD4)4Ccg/0	C57BL/6-Tg(IghelMD4)4Ccg	MGI:3793440
tg39	Tg(IghelMD4)4Ccg/0	involves: C3H/HeJ * C57BL/6	MGI:3694805
tg40	Tg(IghelMD4)4Ccg/0	involves: C57BL/6	MGI:3694806

Allelic Composition	Cd19^tm1(cre)Cgn/Cd19⁺ Pten^tm1Hwu/Pten^tm1Hwu Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19^tm1(cre)Cgn mutation (11 available); any Cd19 mutation (56 available)
Pten^tm1Hwu mutation (16 available); any Pten mutation (81 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal peripheral B cell anergy ( J:155314 )

• failed tolerance induction resulting in abundant autoantibody production, indicating that B cells are prevented from acquiring an anergic state

• B cells have about 10 fold lower bound HEL than in mutants with intact Pten, suggesting that receptor occupancy is reduced on mutant autoreactive B cells and that less HEL is available in adults for inducing and sustaining anergy

• increasing the concentration of free self-antigen confers an anergic phenotype on mutant B cells, but they remain long-lived

Allelic Composition	Dicer1^tm1Mmk/Dicer1^tm1Mmk Tg(IghelMD4)4Ccg/0 Cd79a^tm1(cre)Reth/Cd79a⁺
Genetic Background	involves: BALB/c

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd79a^tm1(cre)Reth mutation (3 available); any Cd79a mutation (22 available)
Dicer1^tm1Mmk mutation (0 available); any Dicer1 mutation (94 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

hematopoietic system

decreased B cell number ( J:135783 )

• while B cell numbers are increased relative to in Cd79a^tm1(cre)Reth/Cd79a⁺ Dicer1^tm1Mmk/Dicer1^tm1Mmk mice, numbers are still lower than in wild-type mice

decreased mature B cell number ( J:135783 )

Allelic Composition	Cd79a^tm1(cre)Reth/Cd79a⁺ Phf5a^tm1.1Oux/Phf5a^tm1.1Oux Tg(IghelMD4)4Ccg/0
Genetic Background	involves: BALB/c * C57BL/6 * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd79a^tm1(cre)Reth mutation (3 available); any Cd79a mutation (22 available)
Phf5a^tm1.1Oux mutation (0 available); any Phf5a mutation (18 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell number ( J:345807 )

• addition of a pre-rearranged IgH transgene partially but significantly increases the number of CD19+ B cells

immune system

abnormal B cell number ( J:345807 )

• addition of a pre-rearranged IgH transgene partially but significantly increases the number of CD19+ B cells

Allelic Composition	Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0
Genetic Background	C57BL/6-Tg(IghelMD4)4Ccg Tg(KLK4mHEL)6Ccg

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(KLK4mHEL)6Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased B cell number ( J:89156 )

• the number of B cells found in the spleen is decreased by more than 6-fold compared to mice carrying just the antibody transgene

• very few of the remaining B cells expresses the transgenic antibody

• the number of B cells expressing the transgenic antibody is 5.9% of that found in singly transgenic NOD mice carrying only the antibody transgene

hematopoietic system

decreased B cell number ( J:89156 )

• the number of B cells found in the spleen is decreased by more than 6-fold compared to mice carrying just the antibody transgene

• very few of the remaining B cells expresses the transgenic antibody

• the number of B cells expressing the transgenic antibody is 5.9% of that found in singly transgenic NOD mice carrying only the antibody transgene

Allelic Composition	Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	C57BL/6-Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal immature B cell morphology ( J:109923 )

• there are reduced numbers of immature B cells bearing transgenic IgM in the bone marrow

• expression of IgM is 2- to 5- fold lower than on immature B cells found in mice carrying just the Tg(IghelMD4)4Ccg

abnormal pro-B cell morphology ( J:109923 )

• there are reduced numbers of pro-B cells bearing transgenic IgM in the bone marrow

decreased B cell number ( J:89156 )

• almost all of the B cells express the transgenic antibody

• the number of B cells expressing the transgenic antibody is 30% less than found in singly transgenic mice carrying just the antibody transgene

• this demonstrates that partial deletion occurs of B cells that recognize soluble self antigen

abnormal mature B cell morphology ( J:109923 )

• there is an increased percentage of mature B cells in the bone marrow

abnormal germinal center B cell morphology ( J:109923 )

• B cells expressing the transgenic Ig are absent from the germinal center of the spleen and lymph nodes

abnormal plasma cell morphology ( J:109923 )

• plasma cells producing transgenic Ig are found rarely in these mice

abnormal spleen B cell follicle morphology ( J:109923 )

• splenic B cells bearing transgenic Ig are restricted to the follicular mantle zones

decreased IgM level ( J:78308 , J:89156 )

• very little IgM is detectable in the sera despite the presence of soluble antigen for the transgenic antibody (J:89156)

abnormal B cell anergy ( J:78308 , J:89156 )

• B cells expressing the transgenic antibody are anergic as determined by the lack of HEL specific antibody found in the sera (J:89156)

• anergy occurs both centrally (i.e. in the bone marrow) and in the periphery (J:89156)

• this anergy can be reversed by stimulating B cells in vitro (J:89156)

abnormal central B cell anergy ( J:109923 )

• immature B cells in the bone marrow lack reactivity when transferred to irradiated host mice that are immunized with a HEL antigen

hematopoietic system

abnormal immature B cell morphology ( J:109923 )

• there are reduced numbers of immature B cells bearing transgenic IgM in the bone marrow

• expression of IgM is 2- to 5- fold lower than on immature B cells found in mice carrying just the Tg(IghelMD4)4Ccg

abnormal pro-B cell morphology ( J:109923 )

• there are reduced numbers of pro-B cells bearing transgenic IgM in the bone marrow

decreased B cell number ( J:89156 )

• almost all of the B cells express the transgenic antibody

• the number of B cells expressing the transgenic antibody is 30% less than found in singly transgenic mice carrying just the antibody transgene

• this demonstrates that partial deletion occurs of B cells that recognize soluble self antigen

abnormal mature B cell morphology ( J:109923 )

• there is an increased percentage of mature B cells in the bone marrow

abnormal germinal center B cell morphology ( J:109923 )

• B cells expressing the transgenic Ig are absent from the germinal center of the spleen and lymph nodes

abnormal plasma cell morphology ( J:109923 )

• plasma cells producing transgenic Ig are found rarely in these mice

abnormal spleen B cell follicle morphology ( J:109923 )

• splenic B cells bearing transgenic Ig are restricted to the follicular mantle zones

decreased IgM level ( J:78308 , J:89156 )

• very little IgM is detectable in the sera despite the presence of soluble antigen for the transgenic antibody (J:89156)

Allelic Composition	Tlr4^Lps-d/Tlr4^Lps-d Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C3H/HeJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(KLK4mHEL)6Ccg mutation (2 available)
Tlr4^Lps-d mutation (7 available); any Tlr4 mutation (90 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

increased B-1 B cell number ( J:115059 )

• more B1 cells are detected among peritoneal lymphocytes in double transgenic mutants compared to Tg(IghelMD4)4Ccg single transgenic mutants

decreased B cell number ( J:115059 )

• a 35% reduction in number of recirculating mature autoreactive B cells is observed, compared to wild-type

immune system

increased B-1 B cell number ( J:115059 )

• more B1 cells are detected among peritoneal lymphocytes in double transgenic mutants compared to Tg(IghelMD4)4Ccg single transgenic mutants

decreased B cell number ( J:115059 )

• a 35% reduction in number of recirculating mature autoreactive B cells is observed, compared to wild-type

Allelic Composition	Lyn^tm1Ard/Lyn⁺ Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyn^tm1Ard mutation (6 available); any Lyn mutation (64 available)
Ptpn6^me-v mutation (3 available); any Ptpn6 mutation (47 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal mature B cell morphology ( J:110531 )

• mature B cells have about 4-fold lower surface IgM levels

immune system

abnormal mature B cell morphology ( J:110531 )

• mature B cells have about 4-fold lower surface IgM levels

Allelic Composition	Tlr9^tm1Aki/Tlr9^tm1Aki Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(KLK4mHEL)6Ccg mutation (2 available)
Tlr9^tm1Aki mutation (7 available); any Tlr9 mutation (65 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased mature B cell number ( J:115059 )

• a 37% reduction in number of recirculating mature autoreactive B cells is observed compared to wild-type

increased B-1 B cell number ( J:115059 )

• more B1 cells are detected among peritoneal lymphocytes in double transgenic mutants compared to Tg(IghelMD4)4Ccg single transgenic mutants

immune system

decreased mature B cell number ( J:115059 )

• a 37% reduction in number of recirculating mature autoreactive B cells is observed compared to wild-type

increased B-1 B cell number ( J:115059 )

• more B1 cells are detected among peritoneal lymphocytes in double transgenic mutants compared to Tg(IghelMD4)4Ccg single transgenic mutants

Allelic Composition	Myd88^tm1Aki/Myd88^tm1Aki Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myd88^tm1Aki mutation (9 available); any Myd88 mutation (50 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(KLK4mHEL)6Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased mature B cell number ( J:115059 )

• a reduction in number of recirculating mature autoreactive B cells is observed compared to wild-type

increased B-1 B cell number ( J:115059 )

• more B1 cells are detected among peritoneal lymphocytes in double transgenic mutants compared to Tg(IghelMD4)4Ccg single transgenic mutants

immune system

decreased mature B cell number ( J:115059 )

• a reduction in number of recirculating mature autoreactive B cells is observed compared to wild-type

increased B-1 B cell number ( J:115059 )

• more B1 cells are detected among peritoneal lymphocytes in double transgenic mutants compared to Tg(IghelMD4)4Ccg single transgenic mutants

Allelic Composition	Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkcd^tm1Qxu mutation (0 available); any Prkcd mutation (52 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(KLK4mHEL)6Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:76134 )

N	• mice exhibit the same number of B cells as in Tg(IghelMD4)4Ccg mice indicating normal clonal deletion

Allelic Composition	Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:76134 )

N	• B cell activation in vitro and in vivo are normal

decreased B cell number ( J:76134 )

• peripheral B cells are reduced compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal spleen primary B follicle morphology ( J:76134 )

• the number of B follicles is increased compared to in Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

increased spleen germinal center number ( J:76134 )

abnormal B cell physiology ( J:76134 )

• surface levels of IgMa are decreased 2-fold compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal B cell proliferation ( J:76134 )

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

abnormal self tolerance ( J:76134 )

• B cells switch to an autoreactive phenotype and fail to induce tolerance

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

autoimmune response ( J:76134 )

• mice exhibit an 80-fold increase in HEL-specific antibodies compared to in Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

hematopoietic system

decreased B cell number ( J:76134 )

• peripheral B cells are reduced compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal spleen primary B follicle morphology ( J:76134 )

• the number of B follicles is increased compared to in Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

increased spleen germinal center number ( J:76134 )

abnormal B cell physiology ( J:76134 )

• surface levels of IgMa are decreased 2-fold compared to in Prkcd^tm1Qxu/Prkcd^tm1Qxu Tg(IghelMD4)4Ccg mice

abnormal B cell proliferation ( J:76134 )

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

cellular

abnormal B cell proliferation ( J:76134 )

• B cells proliferate vigorously in response to HEL unlike Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice

Allelic Composition	Lyn^tm1Ard/Lyn^tm1Ard Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyn^tm1Ard mutation (6 available); any Lyn mutation (64 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell negative selection ( J:110531 )

• when mutant bone marrow is transferred to mice expressing hen egg lysozyme, B cell maturation is blocked at the immature B cell stage instead of anergy inducation in mature B cells as occurs with wild-type bone marrow

abnormal mature B cell morphology ( J:110531 )

• mature B cells have 4-5 lower sIgM levels on their surface

absent B-1 B cells ( J:110531 )

• B1 B cells are absent in these mice

abnormal B cell activation ( J:110531 )

• B cells have altered kinetics of calcium fluxing upon BCR engagement with delayed influx but an ultimately higher peak intracellular concentration

increased IgM level ( J:110531 )

• hypersecretion of the transgenic IgM antibody occurs in these mice

hematopoietic system

abnormal B cell negative selection ( J:110531 )

abnormal mature B cell morphology ( J:110531 )

• mature B cells have 4-5 lower sIgM levels on their surface

absent B-1 B cells ( J:110531 )

• B1 B cells are absent in these mice

abnormal B cell activation ( J:110531 )

• B cells have altered kinetics of calcium fluxing upon BCR engagement with delayed influx but an ultimately higher peak intracellular concentration

increased IgM level ( J:110531 )

• hypersecretion of the transgenic IgM antibody occurs in these mice

Allelic Composition	Lyn^tm1Ard/Lyn⁺ Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyn^tm1Ard mutation (6 available); any Lyn mutation (64 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal mature B cell morphology ( J:110531 )

• mature B cells have about 2-fold lower surface IgM levels

hematopoietic system

abnormal mature B cell morphology ( J:110531 )

• mature B cells have about 2-fold lower surface IgM levels

Allelic Composition	Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ptpn6^me-v mutation (3 available); any Ptpn6 mutation (47 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal mature B cell morphology ( J:110531 )

• mature B cells have about 2-fold lower surface IgM levels

hematopoietic system

abnormal mature B cell morphology ( J:110531 )

• mature B cells have about 2-fold lower surface IgM levels

Allelic Composition	Cd22^tm1Eac/Cd22⁺ Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd22^tm1Eac mutation (1 available); any Cd22 mutation (63 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal mature B cell morphology ( J:110531 )

• expression of CD22 on the surface of cells is half that of controls

• surface expression of IgM is about 70-80% of controls

hematopoietic system

abnormal mature B cell morphology ( J:110531 )

• expression of CD22 on the surface of cells is half that of controls

• surface expression of IgM is about 70-80% of controls

Allelic Composition	Cd22^tm1Eac/Cd22⁺ Lyn^tm1Ard/Lyn⁺ Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd22^tm1Eac mutation (1 available); any Cd22 mutation (63 available)
Lyn^tm1Ard mutation (6 available); any Lyn mutation (64 available)
Ptpn6^me-v mutation (3 available); any Ptpn6 mutation (47 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell negative selection ( J:110531 )

• when mutant bone marrow is transferred to mice expressing hen egg lysozyme, B cell maturation is almost completely blocked at the immature B cell stage

abnormal mature B cell morphology ( J:110531 )

• surface expression of IgM is only 15% of controls

increased IgM level ( J:110531 )

• half the mice have a 15-fold increase in Hel-specific serum IgM

hematopoietic system

abnormal B cell negative selection ( J:110531 )

• when mutant bone marrow is transferred to mice expressing hen egg lysozyme, B cell maturation is almost completely blocked at the immature B cell stage

abnormal mature B cell morphology ( J:110531 )

• surface expression of IgM is only 15% of controls

increased IgM level ( J:110531 )

• half the mice have a 15-fold increase in Hel-specific serum IgM

Allelic Composition	Cd22^tm1Eac/Cd22⁺ Ptpn6^me-v/Ptpn6⁺ Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd22^tm1Eac mutation (1 available); any Cd22 mutation (63 available)
Ptpn6^me-v mutation (3 available); any Ptpn6 mutation (47 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell negative selection ( J:110531 )

• when mutant bone marrow is transferred to mice expressing hen egg lysozyme, B cell maturation is partially blocked at the immature B cell stage

abnormal mature B cell morphology ( J:110531 )

• surface expression of IgM is less than half of controls

hematopoietic system

abnormal B cell negative selection ( J:110531 )

• when mutant bone marrow is transferred to mice expressing hen egg lysozyme, B cell maturation is partially blocked at the immature B cell stage

abnormal mature B cell morphology ( J:110531 )

• surface expression of IgM is less than half of controls

Allelic Composition	Cd22^tm1Eac/Cd22⁺ Lyn^tm1Ard/Lyn⁺ Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell negative selection ( J:110531 )

• when mutant bone marrow is transferred to mice expressing hen egg lysozyme, B cell maturation is patially blocked at the immature B cell stage

abnormal mature B cell morphology ( J:110531 )

• surface expression of IgM is less than half of controls

hematopoietic system

abnormal B cell negative selection ( J:110531 )

• when mutant bone marrow is transferred to mice expressing hen egg lysozyme, B cell maturation is patially blocked at the immature B cell stage

abnormal mature B cell morphology ( J:110531 )

• surface expression of IgM is less than half of controls

Allelic Composition	Cd19^tm1(cre)Cgn/Cd19^tm1(cre)Cgn Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19^tm1(cre)Cgn mutation (11 available); any Cd19 mutation (56 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased B cell number ( J:155314 )

• modest reduction in peripheral B cell numbers

immune system

immune system phenotype ( J:155314 )

N	• tolerance mechanisms to prevent autoantibody production are intact and B cells are able to selectively downregulate IgM

decreased B cell number ( J:155314 )

• modest reduction in peripheral B cell numbers

Allelic Composition	Cd19^tm1(cre)Cgn/Cd19^tm1(cre)Cgn Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19^tm1(cre)Cgn mutation (11 available); any Cd19 mutation (56 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased B cell number ( J:155314 )

• low splenic and lymph node B cell numbers

immune system

immune system phenotype ( J:155314 )

N	• tolerance mechanisms to prevent autoantibody production are intact and B cells are able to selectively downregulate IgM

decreased B cell number ( J:155314 )

• low splenic and lymph node B cell numbers

Allelic Composition	Card19^tm1Rwen/Card19^tm1Rwen Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Card19^tm1Rwen mutation (0 available); any Card19 mutation (13 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal B cell activation ( J:336763 )

• BCR-induced IkappaBalpha phosporylation is increased in B cells

immune system

abnormal B cell activation ( J:336763 )

• BCR-induced IkappaBalpha phosporylation is increased in B cells

Allelic Composition	Card19^tm1Rwen/Card19^tm1Rwen Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6JSfd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Card19^tm1Rwen mutation (0 available); any Card19 mutation (13 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased B cell apoptosis ( J:336763 )

• increased apoptosis of B cells in response to self-antigen HEL or anti-IgM stimulation

decreased B cell proliferation ( J:336763 )

• self-reactive B-cells are less proliferative in response to BCR engagement

decreased B cell number ( J:336763 )

• decrease in the B cell population in the spleen indicating enhanced B cell tolerance

increased B cell number ( J:336763 )

• increase in the frequency of gamma L chain positive splenic B cells compared to transgenic mice wild-type for Card19

hematopoietic system

increased B cell apoptosis ( J:336763 )

• increased apoptosis of B cells in response to self-antigen HEL or anti-IgM stimulation

decreased B cell proliferation ( J:336763 )

• self-reactive B-cells are less proliferative in response to BCR engagement

decreased B cell number ( J:336763 )

• decrease in the B cell population in the spleen indicating enhanced B cell tolerance

increased B cell number ( J:336763 )

• increase in the frequency of gamma L chain positive splenic B cells compared to transgenic mice wild-type for Card19

cellular

increased B cell apoptosis ( J:336763 )

• increased apoptosis of B cells in response to self-antigen HEL or anti-IgM stimulation

decreased B cell proliferation ( J:336763 )

• self-reactive B-cells are less proliferative in response to BCR engagement

Allelic Composition	Fnip1^{Gt(RRM154)Byg}/Fnip1^{Gt(RRM154)Byg} Tg(IghelMD4)4Ccg/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fnip1^{Gt(RRM154)Byg} mutation (0 available); any Fnip1 mutation (59 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased immature B cell number ( J:189078 )

• in the bone marrow compared with Fnip1^tm1.2Tes or Fnip1^{Gt(RRM154)Byg} homozygotes

decreased B-1 B cell number ( J:189078 )

absent B-2 B cells ( J:189078 )

absent B cells ( J:189078 )

• absence of peripheral B cells

hematopoietic system

increased immature B cell number ( J:189078 )

• in the bone marrow compared with Fnip1^tm1.2Tes or Fnip1^{Gt(RRM154)Byg} homozygotes

decreased B-1 B cell number ( J:189078 )

absent B-2 B cells ( J:189078 )

absent B cells ( J:189078 )

• absence of peripheral B cells

Allelic Composition	Cd79b^tm1Mnz/Cd79b^tm1Mnz Tg(IghelMD4)4Ccg/0 Tg(Rag2-Igb)1Mnz/0
Genetic Background	involves: 129S1/Sv * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd79b^tm1Mnz mutation (0 available); any Cd79b mutation (36 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(Rag2-Igb)1Mnz mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal immature B cell morphology ( J:78601 )

• increase in apoptosis of immature B cells

arrested B cell differentiation ( J:78601 )

• B cells fail to develop beyond the IgM^low immature B cell stage and no mature recirculating CD25^-IgM^high B cells are seen

immune system

abnormal immature B cell morphology ( J:78601 )

• increase in apoptosis of immature B cells

arrested B cell differentiation ( J:78601 )

• B cells fail to develop beyond the IgM^low immature B cell stage and no mature recirculating CD25^-IgM^high B cells are seen

Allelic Composition	Cd79b^tm1Mnz/Cd79b^tm1Mnz Tg(BCL2)22Wehi/0 Tg(IghelMD4)4Ccg/0 Tg(Rag2-Igb)1Mnz/0
Genetic Background	involves: 129S1/Sv * C57BL/6 * C57BL/6JWehi * SJL/JWehi

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd79b^tm1Mnz mutation (0 available); any Cd79b mutation (36 available)
Tg(BCL2)22Wehi mutation (2 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(Rag2-Igb)1Mnz mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

arrested B cell differentiation ( J:78601 )

• expression of Tg(BCL2)22Wehi rescues immature B cell apoptosis but immature B cell differentiation beyond the IgM^low immature B cell stage is still impaired and no mature recirculating CD25^-IgM^high B cells are seen

immune system

arrested B cell differentiation ( J:78601 )

Allelic Composition	Endou^tm1Tft/Endou^tm1Tft Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	involves: 129S/SvEv * C57BL/6 * C57BL/6JSfd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Endou^tm1Tft mutation (0 available); any Endou mutation (28 available)
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:208364 )

N	• B cell phenotypes observed in Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg mice are normalized

decreased activation-induced B cell apoptosis ( J:208364 )

• absent in high responder mice

increased autoantibody level ( J:208364 )

• increased anti-HEL auto-antibody production compared with control mice

cellular

decreased activation-induced B cell apoptosis ( J:208364 )

• absent in high responder mice

hematopoietic system

decreased activation-induced B cell apoptosis ( J:208364 )

• absent in high responder mice

Allelic Composition	Cr2^{tm2.1(HLA-A)Crr}/Cr2^{tm2.1(HLA-A)Crr} Tg(IghelMD4)4Ccg/?
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cr2^{tm2.1(HLA-A)Crr} mutation (0 available); any Cr2 mutation (84 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell activation ( J:151991 )

• B cells fail to react to sub-optimal doses of antigen coated in C3 complement

• B cells are also unresponsive to a suboptimal level of anti-IgM, anti-CD40, and C3d

hematopoietic system

abnormal B cell activation ( J:151991 )

• B cells fail to react to sub-optimal doses of antigen coated in C3 complement

• B cells are also unresponsive to a suboptimal level of anti-IgM, anti-CD40, and C3d

Allelic Composition	Tg(IghelMD4)4Ccg/? Tg(KLK4mHEL)6Ccg/?
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(KLK4mHEL)6Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased immature B cell number ( J:78307 )

• a small increase occurs in the number of B220^low immature B cells found in the bone marrow

arrested B cell differentiation ( J:78307 )

• the immature B cells found in the bone marrow have low surface expression of IgM suggesting B cells have encountered antigen but failed to develop to a mature phenotype

decreased mature B cell number ( J:78307 )

• mature B cells are reduced 5- to 20- fold in the bone marrow, spleen and lymph node

• the small number of mature B cells present have B cell receptors encoded by endogenous heavy chain

hematopoietic system

increased immature B cell number ( J:78307 )

• a small increase occurs in the number of B220^low immature B cells found in the bone marrow

arrested B cell differentiation ( J:78307 )

• the immature B cells found in the bone marrow have low surface expression of IgM suggesting B cells have encountered antigen but failed to develop to a mature phenotype

decreased mature B cell number ( J:78307 )

• mature B cells are reduced 5- to 20- fold in the bone marrow, spleen and lymph node

• the small number of mature B cells present have B cell receptors encoded by endogenous heavy chain

Allelic Composition	Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased B cell number ( J:76134 )

• peripheral B cells are reduced compared to in Tg(IghelMD4)4Ccg mice

decreased marginal zone B cell number ( J:132538 )

• very few B cells in the spleen have surface markers indicative of the marginal zone phenotype

abnormal spleen primary B follicle morphology ( J:76134 )

• the number of B follicles is decreased compared to in Tg(IghelMD4)4Ccg mice

abnormal B cell physiology ( J:76134 )

• surface levels of IgMa are decreased 7- to 11-fold compared to in Tg(IghelMD4)4Ccg mice

abnormal B cell anergy ( J:73608 , J:132538 )

• B cells from these mice are unable to activate HEL-specific CD4 T cells from Tg(TcrHEL3A9)1Mmd mice when co-cultured together in the presence of HEL protein (J:73608)

• B cells are anergic when stimulated with HEL antigen as determined by reduced antibody production, no upregulation of activation markers CD69 and CD86, and failure to increase size (J:132538)

hematopoietic system

decreased B cell number ( J:76134 )

• peripheral B cells are reduced compared to in Tg(IghelMD4)4Ccg mice

decreased marginal zone B cell number ( J:132538 )

• very few B cells in the spleen have surface markers indicative of the marginal zone phenotype

abnormal spleen primary B follicle morphology ( J:76134 )

• the number of B follicles is decreased compared to in Tg(IghelMD4)4Ccg mice

abnormal B cell physiology ( J:76134 )

• surface levels of IgMa are decreased 7- to 11-fold compared to in Tg(IghelMD4)4Ccg mice

Allelic Composition	Atp11c^ambr/Y Tg(IghelMD4)4Ccg/0
Genetic Background	involves: C57BL/6 * C57BL/6JApb

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Atp11c^ambr mutation (2 available); any Atp11c mutation (16 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased immature B cell number ( J:171924 )

• immature B cell numbers are partially rescued compared to in Atp11c^ambr hemizygotes

decreased pro-B cell number ( J:171924 )

• pro-B cell numbers are partially rescued compared to in Tg(IghelMD4)4Ccg mice

immune system

decreased immature B cell number ( J:171924 )

• immature B cell numbers are partially rescued compared to in Atp11c^ambr hemizygotes

decreased pro-B cell number ( J:171924 )

• pro-B cell numbers are partially rescued compared to in Tg(IghelMD4)4Ccg mice

Allelic Composition	Fnip1^tm1.2Baba/Fnip1^tm1.2Baba Tg(IghelMD4)4Ccg/0
Genetic Background	involves: C57BL/6 * C57BL/6J * FVB/N * SJL

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fnip1^tm1.2Baba mutation (0 available); any Fnip1 mutation (59 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

increased immature B cell number ( J:189078 )

• in the bone marrow compared with Fnip1^tm1.2Tes or Fnip1^{Gt(RRM154)Byg} homozygotes

decreased B-1 B cell number ( J:189078 )

absent B-2 B cells ( J:189078 )

absent B cells ( J:189078 )

• absence of peripheral B cells

immune system

increased immature B cell number ( J:189078 )

• in the bone marrow compared with Fnip1^tm1.2Tes or Fnip1^{Gt(RRM154)Byg} homozygotes

decreased B-1 B cell number ( J:189078 )

absent B-2 B cells ( J:189078 )

absent B cells ( J:189078 )

• absence of peripheral B cells

Allelic Composition	Bcl6^tm1.1Toka/Bcl6^tm1.1Toka Tg(IghelMD4)4Ccg/0
Genetic Background	involves: C57BL/6 * NZB

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Bcl6^tm1.1Toka mutation (1 available); any Bcl6 mutation (55 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

increased activation-induced B cell apoptosis ( J:174014 )

• B cells co-transferred with Tg(TcraTcrb)425Cbn cells into wild-type mice immunized with HEL-ovalbumin exhibit increased apoptosis compared with similarly treated heterozygous cells

abnormal germinal center B cell physiology ( J:174014 )

• B cells co-transferred with Tg(TcraTcrb)425Cbn cells into wild-type mice immunized with HEL-ovalbumin exhibit impaired formation of germinal center B cell populations compared with similarly treated heterozygous cells

cellular

increased activation-induced B cell apoptosis ( J:174014 )

• B cells co-transferred with Tg(TcraTcrb)425Cbn cells into wild-type mice immunized with HEL-ovalbumin exhibit increased apoptosis compared with similarly treated heterozygous cells

hematopoietic system

increased activation-induced B cell apoptosis ( J:174014 )

• B cells co-transferred with Tg(TcraTcrb)425Cbn cells into wild-type mice immunized with HEL-ovalbumin exhibit increased apoptosis compared with similarly treated heterozygous cells

abnormal germinal center B cell physiology ( J:174014 )

Allelic Composition	Tg(IghelMD4)4Ccg/0 Tg(KLK4mHEL)6Ccg/0
Genetic Background	NOD.B6-Tg(IghelMD4)4Ccg Tg(KLK4mHEL)6Ccg/Dvs

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(KLK4mHEL)6Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased B cell number ( J:89156 )

• the number of B cells found in the spleen is decreased by more than 6-fold compared to NOD mice carrying just the antibody transgene

• very few of the remaining B cells expresses the transgenic antibody

• the number of B cells expressing the transgenic antibody is 1.1% of that found in singly transgenic NOD mice carrying only the antibody transgene

hematopoietic system

decreased B cell number ( J:89156 )

• the number of B cells found in the spleen is decreased by more than 6-fold compared to NOD mice carrying just the antibody transgene

• very few of the remaining B cells expresses the transgenic antibody

• the number of B cells expressing the transgenic antibody is 1.1% of that found in singly transgenic NOD mice carrying only the antibody transgene

Allelic Composition	Tg(IghelMD4)4Ccg/0 Tg(ML5sHEL)5Ccg/0
Genetic Background	NOD.B6-Tg(IghelMD4)4Ccg Tg(ML5sHEL)5Ccg/Dvs

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)
Tg(ML5sHEL)5Ccg mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell morphology ( J:89156 )

• there is no decrease in the number of B cells expressing the transgenic antibody compared to singly transgenic NOD mice that carry just the antibody transgene

• this suggests defect in the NOD strain of deleting B cells that recognize soluble self antigens

abnormal B cell clonal deletion ( J:89156 )

• Background Sensitivity: the normal number of B cells found in these mice demonstrate a defect in the NOD strain of deleting B cells that recognize soluble self antigens

decreased IgM level ( J:89156 )

• very little IgM is detectable in the sera despite the presence of soluble antigen for the transgenic antibody

abnormal B cell anergy ( J:89156 )

• B cells expressing the transgenic antibody are anergic as determined by the lack of HEL specific antibody found in the sera

• anergy occurs both centrally (i.e. in the bone marrow) and in the periphery

• this anergy can be reversed by stimulating B cells in vitro

• when compared to singly transgenic controls, antibody production following BCR and CD40 stimulation is less suppressed in doubly transgenic NOD mice than in doubly transgenic C57BL/6 mice (2.6-fold less vs 4.5 fold less)

hematopoietic system

abnormal B cell morphology ( J:89156 )

• there is no decrease in the number of B cells expressing the transgenic antibody compared to singly transgenic NOD mice that carry just the antibody transgene

• this suggests defect in the NOD strain of deleting B cells that recognize soluble self antigens

abnormal B cell clonal deletion ( J:89156 )

• Background Sensitivity: the normal number of B cells found in these mice demonstrate a defect in the NOD strain of deleting B cells that recognize soluble self antigens

decreased IgM level ( J:89156 )

• very little IgM is detectable in the sera despite the presence of soluble antigen for the transgenic antibody

Allelic Composition	Ighm^tm1Cgn/Ighm^tm1Cgn Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg
Genetic Background	NOD.Cg-Ighm^tm1Cgn Tg(IghelMD4)4Ccg/DvsJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ighm^tm1Cgn mutation (17 available); any Ighm mutation (55 available)
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

insulitis ( J:80859 )

• mice have low levels of insulitis starting at 12 weeks of age with a mean disease score of 1.62 compared to non-transgenic NOD mice that have a mean score of 3.01 at this age

decreased CD8-positive, alpha-beta T cell number ( J:80859 )

• the number of CD8 T cells found in the spleen is half that of non-transgenic NOD mice

abnormal mature B cell morphology ( J:80859 )

• the number but not the proportion of B cells found in the spleen is twice that of non-transgenic NOD mice

• all of the B cells express the transgenic IgM B cell receptor specific for hen egg lysozyme

abnormal B lymphocyte antigen presentation ( J:80859 )

• purified B cells fail to stimulate T cells from non-transgenic NOD mice when cultured together in the presence of the diabetes autoantigen GAD

decreased susceptibility to autoimmune diabetes ( J:80859 )

• 16.7% of female mice develop diabetes by 21 weeks of age compared to control NOD mice that have an incidence rate of 95.5% at this age

• mice with lymphosarcomas were not included in the analysis

neoplasm

increased lymphoma incidence ( J:80859 )

• lymphosarcomas are present in the majority of mice with development starting at 20 weeks of age

hematopoietic system

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

decreased CD8-positive, alpha-beta T cell number ( J:80859 )

• the number of CD8 T cells found in the spleen is half that of non-transgenic NOD mice

abnormal mature B cell morphology ( J:80859 )

• the number but not the proportion of B cells found in the spleen is twice that of non-transgenic NOD mice

• all of the B cells express the transgenic IgM B cell receptor specific for hen egg lysozyme

abnormal B lymphocyte antigen presentation ( J:80859 )

• purified B cells fail to stimulate T cells from non-transgenic NOD mice when cultured together in the presence of the diabetes autoantigen GAD

endocrine/exocrine glands

insulitis ( J:80859 )

• mice have low levels of insulitis starting at 12 weeks of age with a mean disease score of 1.62 compared to non-transgenic NOD mice that have a mean score of 3.01 at this age

cellular

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
NOT	type 1 diabetes mellitus	DOID:9744	OMIM:222100	J:80859

Allelic Composition	Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased pro-B cell number ( J:171924 )

decreased pre-B cell number ( J:171924 )

hematopoietic system

decreased pro-B cell number ( J:171924 )

decreased pre-B cell number ( J:171924 )

Allelic Composition	Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg
Genetic Background	NOD.B6-Tg(IghelMD4)4Ccg/DvsJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

decreased CD8-positive, alpha-beta T cell number ( J:80859 )

• the number of CD8 T cells found in the spleen is half that of non-transgenic NOD mice

abnormal mature B cell morphology ( J:80859 , J:89156 )

• the number but not the proportion of B cells found in the spleen is twice that of non-transgenic NOD mice (J:80859)

• 99.3% of these B cells express the transgenic IgM B cell receptor specific for hen egg lysozyme (J:80859)

• 8.4% of the B cells express endogenous IgM indicating there is some escape from allelic exclusion (J:80859)

• 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL) (J:89156)

• 94% of these B cells retain the ability to bind HEL protein (J:89156)

increased susceptibility to autoimmune diabetes ( J:80859 )

• 90% of female mice are diabetic by 30 weeks of age which is similar to the incidence rate in non-transgenic NOD mice

• there is a significant delay in time of onset compared to non-transgenic diabetic mice

hematopoietic system

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

decreased CD8-positive, alpha-beta T cell number ( J:80859 )

• the number of CD8 T cells found in the spleen is half that of non-transgenic NOD mice

abnormal mature B cell morphology ( J:80859 , J:89156 )

• the number but not the proportion of B cells found in the spleen is twice that of non-transgenic NOD mice (J:80859)

• 99.3% of these B cells express the transgenic IgM B cell receptor specific for hen egg lysozyme (J:80859)

• 8.4% of the B cells express endogenous IgM indicating there is some escape from allelic exclusion (J:80859)

• 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL) (J:89156)

• 94% of these B cells retain the ability to bind HEL protein (J:89156)

cellular

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:80859

Allelic Composition	Tg(IghelMD4)4Ccg/0
Genetic Background	C57BL/6-Tg(IghelMD4)4Ccg

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal B cell morphology ( J:78308 , J:109923 , J:132538 )

• most peripheral B cells express the transgenic immunoglobulin (Ig) that binds hen egg lysozyme peptide (HEL) with a high affinity (J:109923)

• the few B cells expressing endogenous Ig in the spleen express high levels of IgM with little to no levels of IgD compared to splenic B cells from wild-type mice that are IgM^low IgD^high (J:109923)

• over 90% of B cells express immunoglobulin that has a high affinity for hen egg lysozyme antigen (HEL) (J:132538)

abnormal immature B cell morphology ( J:109923 )

• there are reduced numbers of immature B cells bearing transgenic IgM in the bone marrow

increased immature B cell number ( J:78308 , J:132538 )

• three quarters of the B cells in the spleen are immature as indicated by the IgM^hi IgD^hi surface markers (J:78308)

• three quarters of the B cells in the spleen are immature as indicated by the IgM^hi IgD^hi surface markers (J:132538)

abnormal pro-B cell morphology ( J:109923 )

• there are reduced numbers of pro-B cells bearing transgenic IgM in the bone marrow

abnormal mature B cell morphology ( J:89156 , J:109923 )

• 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL) (J:89156)

• 94% of these B cells retain the ability to bind HEL protein (J:89156)

• there is an increased percentage of mature B cells in the bone marrow (J:109923)

abnormal B-2 B cell morphology ( J:132538 )

• all of the B cells found in the peritoneal cavity are of the B-2 lineage as determined by B220^hi CD5^lowexpression

abnormal germinal center B cell morphology ( J:109923 )

• B cells expressing the transgenic Ig are absent from the germinal center of the spleen and lymph nodes

abnormal plasma cell morphology ( J:109923 )

• plasma cells bearing endogenous Ig outnumber plasma cells bearing the transgenic Ig

increased IgG2b level ( J:132538 )

• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice

increased IgM level ( J:78308 , J:132538 )

• there are high levels of anti-HEL IgM in the sera of unimmunized mice (J:78308)

• there are high levels of anti-HEL IgM in the sera of unimmunized mice (J:132538)

hematopoietic system

abnormal B cell morphology ( J:78308 , J:109923 , J:132538 )

• most peripheral B cells express the transgenic immunoglobulin (Ig) that binds hen egg lysozyme peptide (HEL) with a high affinity (J:109923)

• over 90% of B cells express immunoglobulin that has a high affinity for hen egg lysozyme antigen (HEL) (J:132538)

abnormal immature B cell morphology ( J:109923 )

• there are reduced numbers of immature B cells bearing transgenic IgM in the bone marrow

increased immature B cell number ( J:78308 , J:132538 )

• three quarters of the B cells in the spleen are immature as indicated by the IgM^hi IgD^hi surface markers (J:78308)

• three quarters of the B cells in the spleen are immature as indicated by the IgM^hi IgD^hi surface markers (J:132538)

abnormal pro-B cell morphology ( J:109923 )

• there are reduced numbers of pro-B cells bearing transgenic IgM in the bone marrow

abnormal mature B cell morphology ( J:89156 , J:109923 )

• 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL) (J:89156)

• 94% of these B cells retain the ability to bind HEL protein (J:89156)

• there is an increased percentage of mature B cells in the bone marrow (J:109923)

abnormal B-2 B cell morphology ( J:132538 )

• all of the B cells found in the peritoneal cavity are of the B-2 lineage as determined by B220^hi CD5^lowexpression

abnormal germinal center B cell morphology ( J:109923 )

• B cells expressing the transgenic Ig are absent from the germinal center of the spleen and lymph nodes

abnormal plasma cell morphology ( J:109923 )

• plasma cells bearing endogenous Ig outnumber plasma cells bearing the transgenic Ig

increased IgG2b level ( J:132538 )

• there are low levels of anti-HEL IgG2b in the sera of unimmunized mice

increased IgM level ( J:78308 , J:132538 )

• there are high levels of anti-HEL IgM in the sera of unimmunized mice (J:78308)

• there are high levels of anti-HEL IgM in the sera of unimmunized mice (J:132538)

Allelic Composition	Tg(IghelMD4)4Ccg/0
Genetic Background	involves: C3H/HeJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased pro-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

decreased pre-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

immune system

decreased pro-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

decreased pre-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

Allelic Composition	Tg(IghelMD4)4Ccg/0
Genetic Background	involves: C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IghelMD4)4Ccg mutation (3 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased pro-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

decreased pre-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

abnormal B cell activation ( J:73608 )

• B cells become activated when cultured with HEL protein and CD4 T cells from Tg(TcrHEL3A9)1Mmd transgenic mice

• B cells in this culture quickly upregulate CD44 and CD86

• by 48 hours in culture, B cells are greatly enlarged as they undergo blastogenesis

• B cells cultured without the T cells initially upregulate activation markers but then die

• when HEL peptide is used in culture, some B cells increase expression of activation markers but do not form large blast cells

hematopoietic system

decreased pro-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

decreased pre-B cell number ( J:115059 )

• bone marrow contains fewer combined numbers of combined pro-pre B cells than on a B6 background

abnormal B cell activation ( J:73608 )

• B cells become activated when cultured with HEL protein and CD4 T cells from Tg(TcrHEL3A9)1Mmd transgenic mice

• B cells in this culture quickly upregulate CD44 and CD86

• by 48 hours in culture, B cells are greatly enlarged as they undergo blastogenesis

• B cells cultured without the T cells initially upregulate activation markers but then die

• when HEL peptide is used in culture, some B cells increase expression of activation markers but do not form large blast cells

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/09/2024 MGI 6.23