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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Mapk8ip1tm1Rjd
targeted mutation 1, Roger J Davis
MGI:2183313
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Mapk8ip1tm1Rjd/Mapk8ip1tm1Rjd involves: 129S6/SvEvTac * C57BL/6 MGI:3618376
cx2
Mapk8ip1tm1Rjd/Mapk8ip1tm1Rjd
Mapk8ip2tm1Rjd/Mapk8ip2tm1Rjd
involves: 129S6/SvEvTac * 129X1/SvJ MGI:4360031
cx3
Mapk8ip1tm1Rjd/Mapk8ip1tm1Rjd
Mapk8ip2tm1Rjd/Mapk8ip2tm1Rjd
involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6 MGI:3759994


Genotype
MGI:3618376
hm1
Allelic
Composition
Mapk8ip1tm1Rjd/Mapk8ip1tm1Rjd
Genetic
Background
involves: 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk8ip1tm1Rjd mutation (2 available); any Mapk8ip1 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• in vitro, mutant hippocampal neurons exhibit reduced stress-induced apoptosis following exposure to the excitotoxin kainate
• mutant neurons are also resistant to the apoptotic effects of anoxic stress induced by oxygen-glucose deprivation, as shown by reduced labeling in TUNEL assays and increased cell survival
• following exposure to kainite-induced excitotoxic stress, mutant hippocampal neurons display attenuated JNK activation despite a normal level of Jun protein expression
• in vivo, homozygotes show a severe reduction in the extent of kainate-induced lesions in the CA3 subfield of the hippocampus, with a reduced distribution of TUNEL-positive and GFAP-positive astroglial fibers relative to wild-type mice
• no differences in JNK activation is observed following exposure to ultraviolet radiation or treatment with the drug anisomycin
• NMDA-mediated current amplitude is decreased relative to in wild-type mice

homeostasis/metabolism
N
• contrary to expectations, homozygotes display normal islet morphology, normal expression of insulin and glucagon, as well as normal blood glucose levels and performance in glucose tolerance tests relative to wild-type mice
• in vitro, mutant hippocampal neurons exhibit reduced stress-induced apoptosis following exposure to the excitotoxin kainate
• mutant neurons are also resistant to the apoptotic effects of anoxic stress induced by oxygen-glucose deprivation, as shown by reduced labeling in TUNEL assays and increased cell survival
• following exposure to kainite-induced excitotoxic stress, mutant hippocampal neurons display attenuated JNK activation despite a normal level of Jun protein expression
• in vivo, homozygotes show a severe reduction in the extent of kainate-induced lesions in the CA3 subfield of the hippocampus, with a reduced distribution of TUNEL-positive and GFAP-positive astroglial fibers relative to wild-type mice
• no differences in JNK activation is observed following exposure to ultraviolet radiation or treatment with the drug anisomycin

cellular
• in vitro, mutant hippocampal neurons exhibit reduced stress-induced apoptosis following exposure to the excitotoxin kainate
• mutant neurons are also resistant to the apoptotic effects of anoxic stress induced by oxygen-glucose deprivation, as shown by reduced labeling in TUNEL assays and increased cell survival
• following exposure to kainite-induced excitotoxic stress, mutant hippocampal neurons display attenuated JNK activation despite a normal level of Jun protein expression
• in vivo, homozygotes show a severe reduction in the extent of kainate-induced lesions in the CA3 subfield of the hippocampus, with a reduced distribution of TUNEL-positive and GFAP-positive astroglial fibers relative to wild-type mice
• no differences in JNK activation is observed following exposure to ultraviolet radiation or treatment with the drug anisomycin




Genotype
MGI:4360031
cx2
Allelic
Composition
Mapk8ip1tm1Rjd/Mapk8ip1tm1Rjd
Mapk8ip2tm1Rjd/Mapk8ip2tm1Rjd
Genetic
Background
involves: 129S6/SvEvTac * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk8ip1tm1Rjd mutation (2 available); any Mapk8ip1 mutation (33 available)
Mapk8ip2tm1Rjd mutation (0 available); any Mapk8ip2 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• within 2 weeks of birth

growth/size/body
N
• mice exhibit normal growth
• at P2 and P10

homeostasis/metabolism
N
• despite abnormal beta cell morphology, mice exhibit normal blood insulin concentration

endocrine/exocrine glands
• beta cells exhibit fewer exocytotic vesicles compared with wild-type cells




Genotype
MGI:3759994
cx3
Allelic
Composition
Mapk8ip1tm1Rjd/Mapk8ip1tm1Rjd
Mapk8ip2tm1Rjd/Mapk8ip2tm1Rjd
Genetic
Background
involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk8ip1tm1Rjd mutation (2 available); any Mapk8ip1 mutation (33 available)
Mapk8ip2tm1Rjd mutation (0 available); any Mapk8ip2 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• mice exhibit defects in the cerebellum
• mice have defects in arborization of Purkinje cell
• however, cortical and hippocampal neurons are normal
• treatment with NMDA fails to cause an increase in cytoplasmic calcium in neurons unlike in wild-type mice
• NMDA-mediated current amplitude is decreased relative to in wild-type mice
• desensitization of the NMDA-mediated current is reduced relative to in wild-type mice
• however, intracellular calcium homeostasis is normal

behavior/neurological
• mice are severely ataxic





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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory