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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Mark2tm1Hpw
targeted mutation 1, Helen Piwnica-Worms
MGI:2182819
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Mark2tm1Hpw/Mark2tm1Hpw involves: 129X1/SvJ * C57BL/6 MGI:3850957
ht2
Mark2tm1Hpw/Mark2+ involves: 129X1/SvJ * C57BL/6 MGI:3850956
cx3
Mark2tm1Hpw/Mark2tm1Hpw
Mark3tm1Hpw/Mark3tm1Hpw
involves: 129X1/SvJ * C57BL/6 MGI:4830569
cx4
Mark2tm1Hpw/Mark2+
Mark3tm1Hpw/Mark3tm1Hpw
involves: 129X1/SvJ * C57BL/6 MGI:4830570
cx5
Mark2tm1Hpw/Mark2tm1Hpw
Mark3tm1Hpw/Mark3+
involves: 129X1/SvJ * C57BL/6 MGI:4830571


Genotype
MGI:3850957
hm1
Allelic
Composition
Mark2tm1Hpw/Mark2tm1Hpw
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mark2tm1Hpw mutation (1 available); any Mark2 mutation (57 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• slightly fewer than expected mice are found at 3 weeks

cellular
• enhanced 4-fold in white adipose tissue and increased in brown adipose tissue
• anti-IgM stimulated B cells exhibit a 2- to 3-fold decrease in proliferation compared with wild-type cells
• anti-CD40 treated splenocytes B220+ splenocytes is slightly enhanced compared to similarly treated wild-type cells

reproductive system
• female mice bred with wild-type mice produce smaller than expected litters

immune system
• in 30% of mice at 7 to 12 months
• in older mice
• spleen cellularity is increased 2-fold in older mice compared to in wild-type mice
• mice exhibit an expansion of Ter119+HSA+ lymphocytes on the spleen compared with wild-type mice
• young and old mice exhibit lymph node hyperplasia due to an increase in B220+ cells compared to in wild-type mice
• CD4+ T cells exhibit increased levels of memory markers compared with wild-type cells
• spleens often exhibit follicular expansion due to enlarged follicular cells compared to in wild-type mice
• in 30% of mice at 7 to 12 months
• young and old mice exhibit lymph node hyperplasia due to an increase in B220+ cells compared to in wild-type mice
• at 5 to 12 months, 85% of mice exhibit some immunological disorders unlike wild-type mice
• anti-IgM stimulated B cells exhibit a 2- to 3-fold decrease in proliferation compared with wild-type cells
• anti-CD40 treated splenocytes B220+ splenocytes is slightly enhanced compared to similarly treated wild-type cells
• parietal salivary glands exhibit lymphocytic infiltrates unlike in wild-type mice
• T-cell-dependent IgG1 and IgG2a response to NP-KLH are 7.5-fold and 9.5-fold, respectively, compared with wild-type mice
• 17 days after treatment with NP-Ficoll, mice exhibit 3.5-fold lower serum IgG3 levels compared with similarly treated wild-type mice
• 17 days after treatment with NP-Ficoll, mice exhibit 4-fold lower IgM serum levels compared with similarly treated wild-type mice
• concanavalin A or ati-CD3 antibody treated splenocytes produce 3-fold more IFN-gamma compared with similarly treated wild-type mice
• Th1 cells produce 3-fold more IFN-gamma than wild-type cells
• following TCR cross-linking, T cells produce more IFN-gamma than similarly treated wild-type cells
• Th2 cells produce 3-fold more IL4 than wild-type cells
• following TCR cross-linking, T cells produce more IL4 than similarly treated wild-type cells
• kidneys exhibit lymphocytic infiltrates unlike in wild-type mice
• mice exhibit membranoproliferative glomerulonephritis
• lungs exhibit lymphocytic infiltrates unlike in wild-type mice

homeostasis/metabolism
• 5-fold when mice are fed a high-fat diet
• when mice are fed a high-fat diet
• when mice are fed a high-fat diet
• 27% when fed a high fat diet
• insulin-stimulated glucose infusion rate and turnover are increased compared to in wild-type mice
• mildly under fed conditions
• 5-fold when mice are fed a high-fat diet and also decreased when mice are fed normal chow
• mild to severe in 29% of males and 17% of females
• in 33% of males and 45% of females
• 7% when fed a high fat diet

renal/urinary system
• thickening of the capillary walls
• mild to severe in 29% of males and 17% of females
• in 33% of males and 45% of females
• kidneys exhibit lymphocytic infiltrates unlike in wild-type mice
• mice exhibit membranoproliferative glomerulonephritis
• glomeruli exhibit diffuse mesangial hypercellularity
• glomeruli are hypercellular and lobulated unlike in wild-type mice

adipose tissue
• BAT mass is reduced even when mice are fed a high-fat diet (J:163905)
• in the epididymal fat pads at 12 weeks (J:120128)
• WAT mass is reduced even when mice are fed a high-fat diet (J:163905)
• 14% compared to 23% in wild-type mice
• enhanced 4-fold in white adipose tissue and increased in brown adipose tissue

digestive/alimentary system
• 30% of mice exhibit colorectal prolapse
• 41% of aged females and 18% of aged males exhibit colorectal prolapse
• parietal salivary glands exhibit lymphocytic infiltrates unlike in wild-type mice

growth/size/body
• by 20% at E13.5
• 25% to 30% (J:68830)
• 20% (J:120128)
• after 7 weeks on a high-fat diet, mice do not gain weight
• in 30% of mice at 7 to 12 months
• in older mice
• spleen cellularity is increased 2-fold in older mice compared to in wild-type mice
• mice exhibit an expansion of Ter119+HSA+ lymphocytes on the spleen compared with wild-type mice

endocrine/exocrine glands
• parietal salivary glands exhibit lymphocytic infiltrates unlike in wild-type mice

respiratory system
• lungs exhibit lymphocytic infiltrates unlike in wild-type mice

hematopoietic system
• anti-IgM stimulated B cells exhibit a 2- to 3-fold decrease in proliferation compared with wild-type cells
• anti-CD40 treated splenocytes B220+ splenocytes is slightly enhanced compared to similarly treated wild-type cells
• in 30% of mice at 7 to 12 months
• in older mice
• spleen cellularity is increased 2-fold in older mice compared to in wild-type mice
• mice exhibit an expansion of Ter119+HSA+ lymphocytes on the spleen compared with wild-type mice
• young and old mice exhibit lymph node hyperplasia due to an increase in B220+ cells compared to in wild-type mice
• CD4+ T cells exhibit increased levels of memory markers compared with wild-type cells
• spleens often exhibit follicular expansion due to enlarged follicular cells compared to in wild-type mice
• 17 days after treatment with NP-Ficoll, mice exhibit 3.5-fold lower serum IgG3 levels compared with similarly treated wild-type mice
• 17 days after treatment with NP-Ficoll, mice exhibit 4-fold lower IgM serum levels compared with similarly treated wild-type mice

behavior/neurological
• 2-fold when fed a high fat diet

embryo
• by 20% at E13.5

liver/biliary system
• in aged mice fed normal chow

cardiovascular system
• thickening of the capillary walls




Genotype
MGI:3850956
ht2
Allelic
Composition
Mark2tm1Hpw/Mark2+
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mark2tm1Hpw mutation (1 available); any Mark2 mutation (57 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• in 1 of 8 males
• in 2 of 11 females

digestive/alimentary system
• 3 of 107 mice exhibit colorectal prolapse

growth/size/body
• at 1 year

renal/urinary system
• in 1 of 8 males
• in 2 of 11 females




Genotype
MGI:4830569
cx3
Allelic
Composition
Mark2tm1Hpw/Mark2tm1Hpw
Mark3tm1Hpw/Mark3tm1Hpw
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mark2tm1Hpw mutation (1 available); any Mark2 mutation (57 available)
Mark3tm1Hpw mutation (1 available); any Mark3 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• complete absence of pups and no mutants are seen at E10.5 or E8.5




Genotype
MGI:4830570
cx4
Allelic
Composition
Mark2tm1Hpw/Mark2+
Mark3tm1Hpw/Mark3tm1Hpw
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mark2tm1Hpw mutation (1 available); any Mark2 mutation (57 available)
Mark3tm1Hpw mutation (1 available); any Mark3 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• only 2.7% of the expected 12.5% of mutants were recovered and many die shortly after birth

growth/size/body
• 23% reduction in body weight




Genotype
MGI:4830571
cx5
Allelic
Composition
Mark2tm1Hpw/Mark2tm1Hpw
Mark3tm1Hpw/Mark3+
Genetic
Background
involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mark2tm1Hpw mutation (1 available); any Mark2 mutation (57 available)
Mark3tm1Hpw mutation (1 available); any Mark3 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• only 0.5% of the expected 12.5% of mutants were recovered and many die shortly after birth

growth/size/body
• 30% reduction in body weight





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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory