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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Itgb8tm1Lfr
targeted mutation 1, Louis F Reichardt
MGI:2182548
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Itgb8tm1Lfr/Itgb8tm1Lfr either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J) MGI:3583454
hm2
Itgb8tm1Lfr/Itgb8tm1Lfr involves: 129/Sv * ICR MGI:5008416
cn3
Itgb8tm1Lfr/Itgb8tm2Lfr
Tg(Tek-cre)1Rwng/0
involves: 129/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL MGI:3609118
cn4
Itgb8tm1Lfr/Itgb8tm2Lfr
Neurod6tm1(cre)Kan/Neurod6+
involves: 129/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3609115
cn5
Itgb8tm1Lfr/Itgb8tm2Lfr
Tg(Nes-cre)1Kln/0
involves: 129/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3609116
cn6
Itgb8tm1Lfr/Itgb8tm2Lfr
Tg(Nes-cre)1Wmz/0
involves: 129/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3609117


Genotype
MGI:3583454
hm1
Allelic
Composition
Itgb8tm1Lfr/Itgb8tm1Lfr
Genetic
Background
either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb8tm1Lfr mutation (1 available); any Itgb8 mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• one third of homozygous embryos survive to birth but then die within the first four days after birth
• development is normal to E9.5
• about two thirds of homozygotes die between E10 and E12
• remaining embryos normal
• heart is not beating in about 20% of E10.5 embryos

embryo
• about 50% were smaller than littermate controls
• floor plate of neural tube is absent in mice destined to die by E11.5
• in 50% of mice destined to die by E11.5
• some dilation of blood vessels observed
• primary vascular plexus less complex and with less branching than controls
• confirmed in mice carrying the Tg(TIE2-lacZ)182Sato/0 transgene
• fewer fetal blood vessels or absence of such vessels
• allantoic and fetal placental blood sinuses frequently dilated and filled with fetal red blood cells
• less vascularized than controls
• fewer fetal blood vessels penetrate the layer and sponge-like vascular network missing
• labyrinthine zone of placenta dramatically reduced in thickness
• 5 of 41 viable homozygous embryos show abnormally loose allantois-chorion connections at E10.5

cardiovascular system
• endothelial cells of blood vessels are hyperproliferative
• capillaries fail to penetrate the neuroepithelium from the perineural plexus in embryos that fail to survive to birth
• mice that survive have grossly normal capillaries at E11.5 but hemorrhaging occurs
• capillaries are present as aggregates forming complex structures
• fewer fetal blood vessels or absence of such vessels
• allantoic and fetal placental blood sinuses frequently dilated and filled with fetal red blood cells
• less vascularized than controls
• fewer fetal blood vessels penetrate the layer and sponge-like vascular network missing
• failure of capillary vesseles to penetrate very far into the neuroepithelium
• branching defect but original vasculogenesis is normal
• confirmed in mice carrying the Tg(TIE2-lacZ)182Sato/0 transgene
• primary vascular plexus less complex and with less branching than controls
• some dilation of blood vessels observed
• confirmed in mice carrying the Tg(TIE2-lacZ)182Sato/0 transgene
• in embryos destined to die by E11.5
• ventricles less vascularized
• pericardial cavity often dilated
• in embryos surviving to birth hemorrhage first appears at E12.5 in ganglionic eminence
• spreads to diencephalons, then cortex and mantle layers of hindbrain

nervous system
• in embryos surviving to birth hemorrhage first appears at E12.5 in ganglionic eminence
• spreads to diencephalons, then cortex and mantle layers of hindbrain
• radial glial cells in neuroepithelium become poorly aligned between E10.5 and E12.5
• floor plate of neural tube is absent in mice destined to die by E11.5
• seen in mice that survive to birth
• in mice surviving to birth, bilateral cavitation is found in subventricular areas surrounding all four ventricles by E11.5

growth/size/body
• about 10% of embryos that survive past E11.5 display cleft palate
• about 50% were smaller than littermate controls

craniofacial
• about 10% of embryos that survive past E11.5 display cleft palate

digestive/alimentary system
• about 10% of embryos that survive past E11.5 display cleft palate

homeostasis/metabolism

cellular
• radial glial cells in neuroepithelium become poorly aligned between E10.5 and E12.5
• discontinuous expression of fibronectin and laminin at E14.5 indicates abnormalities in the basement membranes of brain capillaries
• no failure of assembly




Genotype
MGI:5008416
hm2
Allelic
Composition
Itgb8tm1Lfr/Itgb8tm1Lfr
Genetic
Background
involves: 129/Sv * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb8tm1Lfr mutation (1 available); any Itgb8 mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• in newborn mice, brain vessels are enlarged and hyperplastic compared to in control mice
• enlarged ventricles

cardiovascular system
• in newborn mice, brain vessels are enlarged and hyperplastic compared to in control mice




Genotype
MGI:3609118
cn3
Allelic
Composition
Itgb8tm1Lfr/Itgb8tm2Lfr
Tg(Tek-cre)1Rwng/0
Genetic
Background
involves: 129/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb8tm1Lfr mutation (1 available); any Itgb8 mutation (1 available)
Itgb8tm2Lfr mutation (1 available); any Itgb8 mutation (1 available)
Tg(Tek-cre)1Rwng mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• no hemorrhages or other defects are seen in the brain and a normal vascular plexus develops in the brain




Genotype
MGI:3609115
cn4
Allelic
Composition
Itgb8tm1Lfr/Itgb8tm2Lfr
Neurod6tm1(cre)Kan/Neurod6+
Genetic
Background
involves: 129/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb8tm1Lfr mutation (1 available); any Itgb8 mutation (1 available)
Itgb8tm2Lfr mutation (1 available); any Itgb8 mutation (1 available)
Neurod6tm1(cre)Kan mutation (0 available); any Neurod6 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• no hemorrhages or other defects are seen in the brain




Genotype
MGI:3609116
cn5
Allelic
Composition
Itgb8tm1Lfr/Itgb8tm2Lfr
Tg(Nes-cre)1Kln/0
Genetic
Background
involves: 129/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb8tm1Lfr mutation (1 available); any Itgb8 mutation (1 available)
Itgb8tm2Lfr mutation (1 available); any Itgb8 mutation (1 available)
Tg(Nes-cre)1Kln mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• blood vessels in the brain do not run parallel to the astroglia
• vessels in the brain develop large irregular endothelial cell clusters
• at P0 hemorrhages are seen in the dorsal cortex, thalamus, and in the ganglionic eminence near the deep mesencephalic nucleus; however hemorrhages are not seen in adult mutants and no cortical lamination defects are seen in adults
• hemorrhaging is less severe than in mice homozygous for Itgb8tm1Lfr

nervous system
• at P0 hemorrhages are seen in the dorsal cortex, thalamus, and in the ganglionic eminence near the deep mesencephalic nucleus; however hemorrhages are not seen in adult mutants and no cortical lamination defects are seen in adults
• hemorrhaging is less severe than in mice homozygous for Itgb8tm1Lfr
• at E14.5 radial glia near the ganglionic eminence (where hemorrhaging could be seen) are disorganized but those in the developing cortex (where hemorrhages are not yet present) appear normal
• at P0 astroglia appear disorganized and lack the normal parallel organization
• blood vessels in the brain do not run parallel to the astoglia




Genotype
MGI:3609117
cn6
Allelic
Composition
Itgb8tm1Lfr/Itgb8tm2Lfr
Tg(Nes-cre)1Wmz/0
Genetic
Background
involves: 129/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Itgb8tm1Lfr mutation (1 available); any Itgb8 mutation (1 available)
Itgb8tm2Lfr mutation (1 available); any Itgb8 mutation (1 available)
Tg(Nes-cre)1Wmz mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• hemorrhaging is similar to mutants crossed to Tg(Nes-cre)1Kln and less severe than in mice homozygous for Itgb8tm1Lfr

nervous system
• hemorrhaging is similar to mutants crossed to Tg(Nes-cre)1Kln and less severe than in mice homozygous for Itgb8tm1Lfr





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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory