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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Cst3tm1Karl
targeted mutation 1, S Karlsson
MGI:2182140
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Cst3tm1Karl/Cst3tm1Karl involves: 129S/SvEv * C57BL/6 MGI:3621900
hm2
Cst3tm1Karl/Cst3tm1Karl involves: 129S/SvEv * C57BL/6J * CBA MGI:3621825
cx3
Apoetm1Unc/Apoetm1Unc
Cst3tm1Karl/Cst3tm1Karl
involves: 129S/SvEv * C57BL/6 MGI:3621887


Genotype
MGI:3621900
hm1
Allelic
Composition
Cst3tm1Karl/Cst3tm1Karl
Genetic
Background
involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cst3tm1Karl mutation (1 available); any Cst3 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
• after intravenous injection of melanoma cells, Cst3 knockout mice have 7 times fewer metastatic lung colonies than wild-type mice two weeks post injection
• null mice analyzed at times from 15 mnutes to 96 hours post-injection showed fewer melanoma cells as short a time as 1 minutes after injection compared to wild-type; at all time points, nulls showed fewer metatatic colonies/cells than wild-type mice; two weeks after injection, null mice had fewer melanoma colonies spread throughout their lungs than wild-type62407




Genotype
MGI:3621825
hm2
Allelic
Composition
Cst3tm1Karl/Cst3tm1Karl
Genetic
Background
involves: 129S/SvEv * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cst3tm1Karl mutation (1 available); any Cst3 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• in knockouts there is less neuronal damage in the cortex and striatum than in wild-type
• in knockout mice the infarct volume after 40 minutes of MCA occlusion is significantly larger than in wild-type
• global ischemia of 12 minutes caused significantly less neuronal damage in the CA3 region and dentate gyrus of knockout mice than in wild-type

homeostasis/metabolism
• in knockouts there is less neuronal damage in the cortex and striatum than in wild-type
• in knockout mice the infarct volume after 40 minutes of MCA occlusion is significantly larger than in wild-type




Genotype
MGI:3621887
cx3
Allelic
Composition
Apoetm1Unc/Apoetm1Unc
Cst3tm1Karl/Cst3tm1Karl
Genetic
Background
involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apoetm1Unc mutation (25 available); any Apoe mutation (88 available)
Cst3tm1Karl mutation (1 available); any Cst3 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• aortic tissue extracts from double knockouts show higher levels of cathepsins S and L and the long form of cathepsin B than ApoE null controls
• aortic smooth muscle cells exhibit an augmented ability to degrade elastin in vitro

cardiovascular system
• double knockout mice display thinning of the tunica media in the aortic arch compared to ApoE knockouts
• double knockouts show more framentation of elastic laminae than in either the ApoE or Cst3 single knockout mouse
• double knockout mice display thinning of the tunica media in the aortic arch compared to ApoE knockouts
• atherosclerotic lesions from aortas of double knockouts had increased smooth muscle cell content (9.3% vs. 4.2% positive lesion area) and collagen (14.4% vs. 4.6%) compared to ApoE knockout mice; fibrous caps develop significantly more in double mutants than controls
• in vitro double knockout smooth muscle cells proliferate more rapidly than control cells which might contribute to the increase in SMC and collagen content in aortic lesions

muscle
• in vitro double knockout smooth muscle cells proliferate more rapidly than control cells which might contribute to the increase in SMC and collagen content in aortic lesions





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
12/04/2018
MGI 6.13
The Jackson Laboratory