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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Nodaltm1Rob
targeted mutation 1, Elizabeth J Robertson
MGI:2180793
Summary 27 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Nodaltm1Rob/Nodaltm1Rob involves: 129S/SvEv MGI:2182451
ht2
Nodaltm1Rob/Nodal+ involves: 129S/SvEv MGI:3625855
ht3
Nodaltm1Rob/Nodaltm2Rob involves: 129S/SvEv * ICR MGI:3851002
cn4
Meox2tm1(cre)Sor/Meox2+
Nodaltm1Rob/Nodaltm5Rob
involves: 129S/SvEv * 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 MGI:3056368
cn5
Nodaltm1Rob/Nodaltm5Rob
Tg(Sox2-cre)1Amc/0
involves: 129S/SvEv * C57BL/6 MGI:3056367
cn6
Nodaltm1Rob/Nodal+
Tg(Sox2-cre)1Amc/0
Tgif1tm1Caw/Tgif1tm1Caw
Tgif2tm1Dwot/Tgif2tm1Dwot
involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA MGI:4437426
cn7
Eomestm1Rob/Eomes+
Nodaltm1Rob/Nodal+
Tg(Sox2-cre)1Amc/0
involves: 129S/SvEv * C57BL/6 * CBA MGI:3775737
cx8
Acvr1ctm1Cfi/Acvr1c+
Gdf1tm1Sjl/Gdf1+
Nodaltm1Rob/Nodal+
involves: 129 * C57BL/6 MGI:3625850
cx9
Cer1tm1Bhr/Cer1tm1Bhr
Lefty1tm1Hmd/Lefty1tm1Hmd
Nodaltm1Rob/Nodal+
involves: 129P2/OlaHsd * 129S/SvEv * 129S7/SvEvBrd * CD-1 MGI:3775813
cx10
Lefty2tm1Hmd/Lefty2tm1Hmd
Nodaltm1Rob/Nodal+
involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6Cr MGI:3720060
cx11
Acvr1ctm1Cfi/Acvr1ctm1Cfi
Nodaltm1Rob/Nodal+
involves: 129P2/OlaHsd * 129/Sv MGI:3512303
cx12
Nodaltm1Rob/Nodal+
Nogtm1Amc/Nogtm1Amc
involves: 129S/SvEv * 129S1/Sv MGI:4819102
cx13
Chrdtm1Emdr/Chrdtm1Emdr
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ MGI:4819097
cx14
Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ MGI:3625856
cx15
Gdf1tm1Sjl/Gdf1tm1Sjl
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3625849
cx16
Acvr2atm1Hsch/Acvr2atm1Hsch
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S1/Sv * C57BL/6J MGI:3696946
cx17
Nodaltm1Rob/Nodaltm2Rob
Trim33tm1.2Los/Trim33tm1.2Los
involves: 129S/SvEv * 129S2/SvPas * C57BL/6 MGI:4830331
cx18
Nodaltm1Rob/Nodal+
Trim33tm1.2Los/Trim33tm1.2Los
involves: 129S/SvEv * 129S2/SvPas * C57BL/6 MGI:4830333
cx19
Smad2tm1Enl/Smad2+
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S4/SvJae MGI:2182498
cx20
Drap1tm1Mms/Drap1tm1Mms
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S6/SvEvTac MGI:3850317
cx21
Eomestm1.1Rob/Eomes+
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S7/SvEvBrd * C57BL/6 * CBA MGI:3775732
cx22
Cer1tm1Bhr/Cer1tm1Bhr
Lefty1tm1Sla/Lefty1tm1Sla
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129S7/SvEvBrd * CD-1 MGI:3775810
cx23
Nodaltm1Rob/Nodal+
Tgif1tm1Dwot/Tgif1tm1Dwot
Tgif2tm1Dwot/Tgif2tm1Dwot
involves: 129S/SvEv * 129X1/SvJ * C57BL/6 MGI:4437425
cx24
Col2a1tm1.1Ksec/Col2a1tm1.1Ksec
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129X1/SvJ * C57BL/6 MGI:4830266
cx25
Col2a1tm1.1Ksec/Col2a1+
Nodaltm1Rob/Nodal+
involves: 129S/SvEv * 129X1/SvJ * C57BL/6 MGI:4830267
cx26
Nodaltm1Rob/Nodal+
Smad2tm1.1Epb/Smad2+
involves: 129S/SvEv * 129X1/SvJ * C57BL/6J * SJL MGI:5791937
cx27
Acvr1btm1Enl/Acvr1b+
Gdf1tm1Sjl/Gdf1+
Nodaltm1Rob/Nodal+
involves: 129/Sv * C57BL/6 MGI:3625853


Genotype
MGI:2182451
hm1
Allelic
Composition
Nodaltm1Rob/Nodaltm1Rob
Genetic
Background
involves: 129S/SvEv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• phenotype is stated to be identical to that of NodalTg(MPSVNeo)413.dRob homozygotes; however no data are presented in J:32935

embryo

growth/size/body




Genotype
MGI:3625855
ht2
Allelic
Composition
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype




Genotype
MGI:3851002
ht3
Allelic
Composition
Nodaltm1Rob/Nodaltm2Rob
Genetic
Background
involves: 129S/SvEv * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Nodaltm2Rob mutation (0 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• in some mice
• severely affected mice exhibit a decrease in midline tissue compared with wild-type mice due to abnormal gastrulation movements
• at E8.5, mice exhibit a reduction in anterior neural tissues compared with wild-type mice
• in some mice
• fused somites
• constriction at the boundary in 40% of mice at E6.5
• at E6.5, 40% of embryos are abnormal with thickened patch of visceral endoderm characteristic of the anterior visceral endoderm

cardiovascular system
• in some mice




Genotype
MGI:3056368
cn4
Allelic
Composition
Meox2tm1(cre)Sor/Meox2+
Nodaltm1Rob/Nodaltm5Rob
Genetic
Background
involves: 129S/SvEv * 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Meox2tm1(cre)Sor mutation (2 available); any Meox2 mutation (9 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Nodaltm5Rob mutation (0 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• development was variable
• fail to rotate their proximal distal axis
• variable amounts of anterior truncation at E6.5
• in severe cases an elongate morphology at E6.5
• profound anterior truncations by E8.5
• embryos occasionally protrude outside the yolk sac at E6.5




Genotype
MGI:3056367
cn5
Allelic
Composition
Nodaltm1Rob/Nodaltm5Rob
Tg(Sox2-cre)1Amc/0
Genetic
Background
involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Nodaltm5Rob mutation (0 available); any Nodal mutation (18 available)
Tg(Sox2-cre)1Amc mutation (5 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• lacked mesoderm at gastrulation
• thickened distal visceral endoderm




Genotype
MGI:4437426
cn6
Allelic
Composition
Nodaltm1Rob/Nodal+
Tg(Sox2-cre)1Amc/0
Tgif1tm1Caw/Tgif1tm1Caw
Tgif2tm1Dwot/Tgif2tm1Dwot
Genetic
Background
involves: 129S/SvEv * C57BL/6 * C57BL/6J * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Tgif1tm1Caw mutation (1 available); any Tgif1 mutation (33 available)
Tgif2tm1Dwot mutation (0 available); any Tgif2 mutation (39 available)
Tg(Sox2-cre)1Amc mutation (5 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• reduced Nodal levels partially rescues ventral neurepithelium morphogenesis observed in double mutants with disorganized neuroepithelium
• reduced Nodal levels partially rescues ventral forebrain morphology observed in double mutants with less disorganization and larger size
• however, forebrain cell proliferation is restored
• in mice that survive to E18.5
• in two-thirds of mice

embryo
• expression analysis suggests there are some mild defects in left right patterning
• severe anterior truncation in a small proportion of mice
• in 2 embryos with severe anterior truncation
• reduced Nodal levels partially rescues ventral neurepithelium morphogenesis observed in double mutants with disorganized neuroepithelium

growth/size/body
• in 2 embryos with severe anterior truncation

cardiovascular system
N
• unlike in double null mice wild-type for Nodal, heart looping morphogenesis is normal




Genotype
MGI:3775737
cn7
Allelic
Composition
Eomestm1Rob/Eomes+
Nodaltm1Rob/Nodal+
Tg(Sox2-cre)1Amc/0
Genetic
Background
involves: 129S/SvEv * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Eomestm1Rob mutation (0 available); any Eomes mutation (25 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Tg(Sox2-cre)1Amc mutation (5 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• severely affected embryos are not recovered

embryo
• embryos with anterior axis truncations are recovered at low frequency




Genotype
MGI:3625850
cx8
Allelic
Composition
Acvr1ctm1Cfi/Acvr1c+
Gdf1tm1Sjl/Gdf1+
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Acvr1ctm1Cfi mutation (1 available); any Acvr1c mutation (18 available)
Gdf1tm1Sjl mutation (1 available); any Gdf1 mutation (5 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype




Genotype
MGI:3775813
cx9
Allelic
Composition
Cer1tm1Bhr/Cer1tm1Bhr
Lefty1tm1Hmd/Lefty1tm1Hmd
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129P2/OlaHsd * 129S/SvEv * 129S7/SvEvBrd * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cer1tm1Bhr mutation (0 available); any Cer1 mutation (4 available)
Lefty1tm1Hmd mutation (1 available); any Lefty1 mutation (16 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• all triple mutant embryos at 7.5 dpc show morphological defects similar to or less severe than Cer1 Lefty1 double mutants




Genotype
MGI:3720060
cx10
Allelic
Composition
Lefty2tm1Hmd/Lefty2tm1Hmd
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6Cr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lefty2tm1Hmd mutation (1 available); any Lefty2 mutation (13 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

embryo
• defects in embryogenesis due to a lack of Lefty2 are partially rescued although mice die in late gastrulation




Genotype
MGI:3512303
cx11
Allelic
Composition
Acvr1ctm1Cfi/Acvr1ctm1Cfi
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129P2/OlaHsd * 129/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Acvr1ctm1Cfi mutation (1 available); any Acvr1c mutation (18 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• expected numbers of compound mutants are detected at weaning




Genotype
MGI:4819102
cx12
Allelic
Composition
Nodaltm1Rob/Nodal+
Nogtm1Amc/Nogtm1Amc
Genetic
Background
involves: 129S/SvEv * 129S1/Sv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Nogtm1Amc mutation (2 available); any Nog mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
N
• no defects are detected in anterior midline tissues




Genotype
MGI:4819097
cx13
Allelic
Composition
Chrdtm1Emdr/Chrdtm1Emdr
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chrdtm1Emdr mutation (1 available); any Chrd mutation (20 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• in 14 of 19 mice with defects in anterior midline tissues

embryo
• expression analysis indicates defects in patterning and function in the anterior most axial mesendoderm
• 23% (19 of 83) show defects in anterior midline tissues
• 14 of these 19 show holoprosencephaly in association with anterior body truncation and fused first pharyngeal arches
• fused in 14 of 19 mice with defects in anterior midline tissues
• expression analysis indicates impairment in ADE specification
• expression analysis indicates defects in patterning and function

cardiovascular system
• cardiac laterality defects are seen in 5 mice

craniofacial
• fused in 14 of 19 mice with defects in anterior midline tissues




Genotype
MGI:3625856
cx14
Allelic
Composition
Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxa2tm1Jrt mutation (3 available); any Foxa2 mutation (16 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• in embryos with loss of left-right asymmetry in Nodal expression the direction of turning is randomized
• seen in all embryos

growth/size/body
• in 7 of 10 mutants the positioning of the abdominal viscera and heart is abnormal




Genotype
MGI:3625849
cx15
Allelic
Composition
Gdf1tm1Sjl/Gdf1tm1Sjl
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gdf1tm1Sjl mutation (1 available); any Gdf1 mutation (5 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

embryo
• defects are seen in structures arising from the first but not the second branchial arch
• at E9.0, the first branchial arch is fused, lacking a midline division
• primitive streak elongation is normal but significant reduction in the number of axial mesendoderm cells is detected in about 50% of mutants
• fail to develop anterior neural folds
• anterior defects only
• absent at E8.5 in the most severely affected embryos
• at E9.0 expression of Shh, a marker of the notochord at this stage, is anteriorly truncated
• the prechordal plate marker Gsc is significantly downregulated at the late headfold stage and the prechordal plate is absent in severely affected embryos

craniofacial
• severely affected embryos lack jaws
• defects are seen in structures arising from the first but not the second branchial arch
• at E9.0, the first branchial arch is fused, lacking a midline division
• seen in 68% of embryos at E13.5, associated with holoprosencephaly
• severely affected embryos lack the entire tongue while mildly affected embryos lack the distal portion of the tongue
• hypomorphic nasal septum resulting in a fused nasal cavity

nervous system
• anterior defects only
• seen in 68% of embryos at E13.5, associated with gross rostral truncation and cleft lip
• thickening of the diencephalon seen in embryos with holoprosencephaly
• a recessed third ventricle that fails to expand ventrally is seen in embryos with holoprosencephaly

digestive/alimentary system
• severely affected embryos lack the entire tongue while mildly affected embryos lack the distal portion of the tongue
• at E9.0, in most embryos the foregut does not reach into the center of the first branchial arch unlike in wild-type mice

respiratory system
• hypomorphic nasal septum resulting in a fused nasal cavity

skeleton
• severely affected embryos lack jaws

growth/size/body
• seen in 68% of embryos at E13.5, associated with holoprosencephaly
• severely affected embryos lack the entire tongue while mildly affected embryos lack the distal portion of the tongue
• hypomorphic nasal septum resulting in a fused nasal cavity




Genotype
MGI:3696946
cx16
Allelic
Composition
Acvr2atm1Hsch/Acvr2atm1Hsch
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S1/Sv * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Acvr2atm1Hsch mutation (0 available); any Acvr2a mutation (20 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• embryonic lethality although time not specified

embryo
• over 60% show gastrulation defects

nervous system
• 4 of 13 embryos that developed beyond gastrulation have a reduced forebrain

vision/eye
• 6 of 8 newborns display cyclopia

growth/size/body
• 6 of 8 newborns show truncation of rostral head structures




Genotype
MGI:4830331
cx17
Allelic
Composition
Nodaltm1Rob/Nodaltm2Rob
Trim33tm1.2Los/Trim33tm1.2Los
Genetic
Background
involves: 129S/SvEv * 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Nodaltm2Rob mutation (0 available); any Nodal mutation (18 available)
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (42 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
N
• at E6.25 decrease in Nodal expression rescues patterning defects in the anterior visceral endoderm, extraembryonic ectoderm and mesoderm and the decrease in embryo size that are seen in Trim33 single homozygotes




Genotype
MGI:4830333
cx18
Allelic
Composition
Nodaltm1Rob/Nodal+
Trim33tm1.2Los/Trim33tm1.2Los
Genetic
Background
involves: 129S/SvEv * 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (42 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
N
• the decrease in Nodal expression rescues patterning defects in the extraembryonic ectoderm and mesoderm and the decrease in embryo size that are seen in Trim33 single homozygotes




Genotype
MGI:2182498
cx19
Allelic
Composition
Smad2tm1Enl/Smad2+
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Smad2tm1Enl mutation (0 available); any Smad2 mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• at E9.5 11 of 20 embryos had gastrulation defects similar to those in Smad2 single heterozygotes
• 3 of 20 turn in the opposite direction compared to wild-type mice
• 32% (8 of 25) have defects in left-right patterning
• in the most severe cases the rostral head and eyes are truncated
• in affected embryos lateral plate mesoderm is restricted to the posterior region

cardiovascular system
• 3 of 20 have abnormal heart looping
• most common cardiac defect in embryos with left-right patterning abnormalities

growth/size/body
• seen in 6 of 25 embryos, these mice also have transposition of the great arteries

craniofacial
• at E15.5 - E17.5 severe craniofacial defects are seen in 14 of 25 mutants

vision/eye
• present at E15.5 - E17.5 in 9 of 25

respiratory system
• seen in 6 of 25 embryos, these mice also have transposition of the great arteries




Genotype
MGI:3850317
cx20
Allelic
Composition
Drap1tm1Mms/Drap1tm1Mms
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drap1tm1Mms mutation (0 available); any Drap1 mutation (3 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mutant embryos die by E9.5

embryo
• mutant embryos are partially rescued from the mesoderm defects observed in Drap1tm1Mms homozygotes, but are still unable to complete gastrulation
• at E7.5 and E8.25, mutant embryos display a characteristic proximal bulge in the caudal primitive streak




Genotype
MGI:3775732
cx21
Allelic
Composition
Eomestm1.1Rob/Eomes+
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S7/SvEvBrd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Eomestm1.1Rob mutation (0 available); any Eomes mutation (25 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• AVE is correctly induced but fails to migrate anteriorly
• heart patterning abnormalities are observed by E9.5 due to loss of midline structures
• embryos lack visible germ layers
• left-right patterning abnormalities are observed by E9.5 due to loss of midline structures
• floor plate expansion results in increased spacing between somite rows at E9.5
• by E9.5, mutants lack head structures rostral to the otic placodes
• in the most severely affected embryos, relatively complete duplications of the posterior body axis are observed, including extra somite rows
• node abnormalities result in expansion of the floor plate of the neural tube
• node duplications are accompanied by formation of an accessory notochord
• at E8.5, development of the node is severely disturbed in a subset of mutants
• complete node duplications are observed in some mutants
• the most severely affected double heterozygotes show abnormalities around E7 and fail to form a primitive streak
• addition rows of somites are observed in the most severely affected embryos
• by late gastrulation stages, embryos are grossly disorganized
• the most severely affected embryos develop pronounced constrictions between the embryonic and extraembryonic regions of the conceptus
• embryos frequently show tissue accumulation within the amniotic cavity

nervous system
• node abnormalities result in expansion of the floor plate of the neural tube

cellular
• AVE is correctly induced but fails to migrate anteriorly




Genotype
MGI:3775810
cx22
Allelic
Composition
Cer1tm1Bhr/Cer1tm1Bhr
Lefty1tm1Sla/Lefty1tm1Sla
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129S7/SvEvBrd * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cer1tm1Bhr mutation (0 available); any Cer1 mutation (4 available)
Lefty1tm1Sla mutation (0 available); any Lefty1 mutation (16 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• all triple mutant embryos at 7.5 dpc show morphological defects similar to or less severe than Cer1 Lefty1 double mutants




Genotype
MGI:4437425
cx23
Allelic
Composition
Nodaltm1Rob/Nodal+
Tgif1tm1Dwot/Tgif1tm1Dwot
Tgif2tm1Dwot/Tgif2tm1Dwot
Genetic
Background
involves: 129S/SvEv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Tgif1tm1Dwot mutation (0 available); any Tgif1 mutation (33 available)
Tgif2tm1Dwot mutation (0 available); any Tgif2 mutation (39 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
N
• unlike in double null mice wild-type for Nodal, the embryonic cavity has formed by E8.5 and a distinct AP axis is present




Genotype
MGI:4830266
cx24
Allelic
Composition
Col2a1tm1.1Ksec/Col2a1tm1.1Ksec
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col2a1tm1.1Ksec mutation (0 available); any Col2a1 mutation (37 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• the frequency of head abnormalities is not significantly different from Col2a1tm1.1Ksec single homozygotes




Genotype
MGI:4830267
cx25
Allelic
Composition
Col2a1tm1.1Ksec/Col2a1+
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129S/SvEv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col2a1tm1.1Ksec mutation (0 available); any Col2a1 mutation (37 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• the frequency of head abnormalities is not significantly different from Col2a1tm1.1Ksec single heterozygotes




Genotype
MGI:5791937
cx26
Allelic
Composition
Nodaltm1Rob/Nodal+
Smad2tm1.1Epb/Smad2+
Genetic
Background
involves: 129S/SvEv * 129X1/SvJ * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
Smad2tm1.1Epb mutation (1 available); any Smad2 mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• in 1 of 41 mice between E10.5 and E12.5

embryo
• 15 of 41 mice exhibit anterior truncations or a severe growth delay

growth/size/body
• in 1 of 41 mice between E10.5 and E12.5
• 15 of 41 mice exhibit anterior truncations or a severe growth delay

respiratory system

taste/olfaction

vision/eye
• partial failure to separate eyes in 1 of 41 mice between E10.5 and E12.5

craniofacial
• in 1 of 41 mice between E10.5 and E12.5




Genotype
MGI:3625853
cx27
Allelic
Composition
Acvr1btm1Enl/Acvr1b+
Gdf1tm1Sjl/Gdf1+
Nodaltm1Rob/Nodal+
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Acvr1btm1Enl mutation (0 available); any Acvr1b mutation (23 available)
Gdf1tm1Sjl mutation (1 available); any Gdf1 mutation (5 available)
Nodaltm1Rob mutation (3 available); any Nodal mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• 3 of 19 mutants have a single forebrain vesicle

respiratory system
• 3 of 19 mutants have a fused nasal cavity

craniofacial
• 3 of 19 mutants have a fused nasal cavity

growth/size/body
• 3 of 19 mutants have a fused nasal cavity





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last database update
11/29/2016
MGI 6.06
The Jackson Laboratory