Phenotypes associated with this allele

• Allele Symbol: Lefty2^tm1Hmd
• Allele Name: targeted mutation 1, Hiroshi Hamada
• Allele ID: MGI:2179551
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lefty2^tm1Hmd/Lefty2^tm1Hmd	involves: 129P2/OlaHsd * C57BL/6Cr	MGI:3720061
cx2	Lefty2^tm1Hmd/Lefty2^tm1Hmd Nodal^tm1Rob/Nodal^+	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6Cr	MGI:3720060
cx3	Lefty2^tm1Hmd/Lefty2^tm1Hmd Shisa2^tm1Sia/Shisa2^tm1Sia	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3718481

Genotype
MGI:3720061

hm1

• Allelic Composition: Lefty2^tm1Hmd/Lefty2^tm1Hmd
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6Cr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:57907 )

• mice die around E11.5

embryo

absent vitelline blood vessels ( J:57907 )

• at E9.5

abnormal embryo development ( J:57907 )

• mice are abnormal at E10.5

abnormal developmental patterning ( J:57907 )

• at E9.5, mice exhibit no signs of axial patterning

abnormal ectoderm development ( J:57907 )

• at E7.5, ingressing ectoderm is increased

abnormal head fold morphology ( J:57907 )

• at E7.5 the head folds are missing

absent notochord ( J:57907 )

• at E7.5

abnormal primitive streak morphology ( J:57907 )

• at E7.5, the primitive streak is expanded laterally

absent primitive node ( J:57907 )

• at E7.5, the anterior-distal region lacks a distinct node

absent somites ( J:57907 )

• at E7.5

abnormal visceral yolk sac morphology ( J:57907 )

• at E9.5, the endodermal and mesodermal components of the yolk sac are dissociated

failure of chorioallantoic fusion ( J:57907 )

• the chorion fails to fuse to the allantois

cardiovascular system

absent vitelline blood vessels ( J:57907 )

• at E9.5

absent heart ( J:57907 )

• at E7.5, the heart is absent

Genotype
MGI:3720060

cx2

• Allelic Composition: Lefty2^tm1Hmd/Lefty2^tm1Hmd; Nodal^tm1Rob/Nodal⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6Cr

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:57907 )

• mice die during late gastrulation

embryo

abnormal embryo development ( J:57907 )

• defects in embryogenesis due to a lack of Lefty2 are partially rescued although mice die in late gastrulation

Genotype
MGI:3718481

cx3

• Allelic Composition: Lefty2^tm1Hmd/Lefty2^tm1Hmd; Shisa2^tm1Sia/Shisa2^tm1Sia
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

nervous system

nervous system phenotype ( J:122557 )

N • no defects in anterior neuroectoderm development are detected