Phenotypes associated with this allele

• Allele Symbol: Cd44^tm2Kmz
• Allele Name: targeted mutation 2, Katalin Mikecz
• Allele ID: MGI:2178950
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd44^tm2Kmz/Cd44^tm2Kmz	DBA/1LacJ-Cd44^tm2Kmz	MGI:4354411
hm2	Cd44^tm2Kmz/Cd44^tm2Kmz	involves: BALB/c * DBA/1	MGI:4354507
cx3	Cd44^tm2Kmz/Cd44^tm2Kmz Sell^tm2Hyn/Sell^tm2Hyn	involves: 129S2/SvPas * BALB/c * DBA/1	MGI:4354509

Genotype
MGI:4354411

hm1

• Allelic Composition: Cd44^tm2Kmz/Cd44^tm2Kmz
• Genetic Background: DBA/1LacJ-Cd44^tm2Kmz

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased susceptibility to induced arthritis ( J:74268 )

• collagen induced arthritis is less severe than in controls

• incidence is about half that of controls and severity is reduced for mice that do develop arthritis

• administration of LPS causes an increase in incidence and severity but not to the degree of controls

• higher circulating levels of hyaluranic acid are noted in the knockout mice compared to controls after arthritis induction

skeleton

decreased susceptibility to induced arthritis ( J:74268 )

• collagen induced arthritis is less severe than in controls

• incidence is about half that of controls and severity is reduced for mice that do develop arthritis

• administration of LPS causes an increase in incidence and severity but not to the degree of controls

• higher circulating levels of hyaluranic acid are noted in the knockout mice compared to controls after arthritis induction

endocrine/exocrine glands

abnormal involution of the mammary gland ( J:74524 )

♀ • 60-80% of pups die within 2 days of birth

♀ • mammary glands undergo involution within 1-4 days postpartum

♀ • only focal lobuloalveolar expansion of the mammary glands is observed two days after birth compared to engorged lobuloalveoli of controls

♀ • numerous apoptotic cells are observed in mammary glands at 2 days postpartum

reproductive system

abnormal uterus morphology ( J:74524 )

♀ • postpartum involution of the uterus is accelerated in these mice

♀ • 24-36 h postpartum, the size of uterus is reduced to about half that of wild-type animals

♀ • postpartum uteri have thinner walls with epithelial cells displaying a more elongated, columnar morphology with elongated nuclei

♀ • smooth muscle of the postpartnum uterus is disorganized compared to controls

♀ • increased apoptosis is associated with this accelerated apoptosis

integument

abnormal involution of the mammary gland ( J:74524 )

♀ • 60-80% of pups die within 2 days of birth

♀ • mammary glands undergo involution within 1-4 days postpartum

♀ • only focal lobuloalveolar expansion of the mammary glands is observed two days after birth compared to engorged lobuloalveoli of controls

♀ • numerous apoptotic cells are observed in mammary glands at 2 days postpartum

Genotype
MGI:4354507

hm2

• Allelic Composition: Cd44^tm2Kmz/Cd44^tm2Kmz
• Genetic Background: involves: BALB/c * DBA/1

immune system

abnormal leukocyte adhesion ( J:114317 )

• the number of adherent leukocytes observed in the postcapillary venules of inflamed ears is 3-fold less than controls from 1 hour to 24 hours after immunization

impaired leukocyte tethering or rolling ( J:114317 )

• less rolling leukocytes are observed in the postcapillary venules of inflamed ears 24 hours after immunization compared to controls

• velocity of these rolling leukocytes is greater than controls 1, 6, 12, and 24 hours after immunization compared to controls

impaired neutrophil recruitment ( J:114317 )

• about 4-fold less Gr⁺ cells are found in inflamed ears compared to controls

abnormal CD4-positive, alpha-beta T cell physiology ( J:114317 )

• about 2-3 fold less CD4⁺ T cells are recruited to inflamed ears compared to controls

decreased susceptibility to type IV hypersensitivity reaction ( J:114317 )

• OVA-sensitized mice have less swelling 12 to 24 hours after OVA injection in the ear compared to controls

cellular

abnormal leukocyte adhesion ( J:114317 )

• the number of adherent leukocytes observed in the postcapillary venules of inflamed ears is 3-fold less than controls from 1 hour to 24 hours after immunization

impaired leukocyte tethering or rolling ( J:114317 )

• less rolling leukocytes are observed in the postcapillary venules of inflamed ears 24 hours after immunization compared to controls

• velocity of these rolling leukocytes is greater than controls 1, 6, 12, and 24 hours after immunization compared to controls

hematopoietic system

abnormal leukocyte adhesion ( J:114317 )

• the number of adherent leukocytes observed in the postcapillary venules of inflamed ears is 3-fold less than controls from 1 hour to 24 hours after immunization

impaired leukocyte tethering or rolling ( J:114317 )

• less rolling leukocytes are observed in the postcapillary venules of inflamed ears 24 hours after immunization compared to controls

• velocity of these rolling leukocytes is greater than controls 1, 6, 12, and 24 hours after immunization compared to controls

impaired neutrophil recruitment ( J:114317 )

• about 4-fold less Gr⁺ cells are found in inflamed ears compared to controls

abnormal CD4-positive, alpha-beta T cell physiology ( J:114317 )

• about 2-3 fold less CD4⁺ T cells are recruited to inflamed ears compared to controls

Genotype
MGI:4354509

cx3

• Allelic Composition: Cd44^tm2Kmz/Cd44^tm2Kmz; Sell^tm2Hyn/Sell^tm2Hyn
• Genetic Background: involves: 129S2/SvPas * BALB/c * DBA/1

immune system

abnormal leukocyte adhesion ( J:114317 )

• the number of adherent leukocytes observed in the postcapillary venules of inflamed ears is 3-fold less than controls from 1 hour to 24 hours after immunization

impaired leukocyte tethering or rolling ( J:114317 )

• less rolling leukocytes are observed in the postcapillary venules of inflamed ears six hours after immunization compared to controls

• velocity of these rolling leukocytes is greater than controls 24 hours after immunization

impaired neutrophil recruitment ( J:114317 )

• about 4-fold less Gr⁺ cells are found in inflamed ears compared to controls

abnormal CD4-positive, alpha-beta T cell physiology ( J:114317 )

• about 2-3 fold less CD4⁺ T cells are recruited to inflamed ears compared to controls

decreased susceptibility to type IV hypersensitivity reaction ( J:114317 )

• OVA-sensitized mice have less swelling 12 to 24 hours after OVA injection in the ear compared to controls

cellular

abnormal leukocyte adhesion ( J:114317 )

• the number of adherent leukocytes observed in the postcapillary venules of inflamed ears is 3-fold less than controls from 1 hour to 24 hours after immunization

impaired leukocyte tethering or rolling ( J:114317 )

• less rolling leukocytes are observed in the postcapillary venules of inflamed ears six hours after immunization compared to controls

• velocity of these rolling leukocytes is greater than controls 24 hours after immunization

hematopoietic system

abnormal leukocyte adhesion ( J:114317 )

• the number of adherent leukocytes observed in the postcapillary venules of inflamed ears is 3-fold less than controls from 1 hour to 24 hours after immunization

impaired leukocyte tethering or rolling ( J:114317 )

• less rolling leukocytes are observed in the postcapillary venules of inflamed ears six hours after immunization compared to controls

• velocity of these rolling leukocytes is greater than controls 24 hours after immunization

impaired neutrophil recruitment ( J:114317 )

• about 4-fold less Gr⁺ cells are found in inflamed ears compared to controls

abnormal CD4-positive, alpha-beta T cell physiology ( J:114317 )

• about 2-3 fold less CD4⁺ T cells are recruited to inflamed ears compared to controls