Phenotypes associated with this allele

• Allele Symbol: Crhr2^tm1Mpsp
• Allele Name: targeted mutation 1, Mary P Stenzel-Poore
• Allele ID: MGI:2178619
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Crhr2^tm1Mpsp/Crhr2^tm1Mpsp	involves: 129X1/SvJ	MGI:4818664
hm2	Crhr2^tm1Mpsp/Crhr2^tm1Mpsp	involves: 129X1/SvJ * C57BL/6J	MGI:3036708

Genotype
MGI:4818664

hm1

• Allelic Composition: Crhr2^tm1Mpsp/Crhr2^tm1Mpsp
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal social investigation ( J:161776 )

• improved social memory; male mice are able to distinguish between a familiar and unfamiliar individual at interexposure intervals where wild-type mice fail to distinguish

Genotype
MGI:3036708

hm2

• Allelic Composition: Crhr2^tm1Mpsp/Crhr2^tm1Mpsp
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

behavior/neurological

behavior/neurological phenotype ( J:61510 , J:88769 )

N • compared to wild-type mice, homozygous mutant mice displayed normal anxiety responses, normal basal locomotor activity, and a similar reduction in locomotor activity in response to urocortin (J:61510)

N • homozygous mutant mice displayed normal anxiety responses in the light/dark emergence task (S. Coste and T. Phillips, unpublished) (J:88769)

abnormal eating behavior ( J:61510 , J:88769 )

• homozygous mutant mice displayed normal basal feeding, but showed altered feeding responses to urocortin (J:61510)

• both wild-type and homozygous mutant mice exhibited a similar inhibition of feeding immediately following icv urocortin treatment; however, mutant mice recovered to normal intake levels more rapidly (6 hrs post-injection) than wild-type animals which remained suppressed for >10 hrs (J:61510)

• in addition to urocortin-stimulated feeding, food intake was altered following isolation stress (S. Coste and M. Stenzel-Poore, unpublished data) (J:88769)

decreased grooming behavior ( J:61510 )

• homozygotes displayed a significantly reduced self-grooming behavior in a novel open-field

cardiovascular system

cardiovascular system phenotype ( J:61510 )

N • homozygous mutant mice displayed a normal resting heart rate; also, transthoracic echocardiography revealed normal basal left ventricular function

abnormal myocardial fiber physiology ( J:61510 )

• in contrast to wild-type, cardiomyoctes from adult homozygous mutant mice failed to respond to urocortin and corticotropin releasing hormone (CRH) by increasing intracellular cAMP levels

• mutant cardiomyocytes exhibited a normal cAMP response to forskolin

• i.v. urocortin administration increased cardiac function in wild-type to ~2-fold above baseline, but had no effect on homozygous mutant mice

increased systemic arterial blood pressure ( J:61510 )

• compared with wild-type mice, homozygous mutant mice had elevated mean arterial pressure (elevated diastolic and systolic blood pressure)

• in contrast to wild-type mice, systemic urocortin administration failed to decrease mean arterial pressure in homozygous mutant mice

homeostasis/metabolism

abnormal hormone level ( J:61510 )

• homozygous mutant mice displayed normal basal levels of POMC1 (ACTH) and corticosterone

• homozygous mutant mice displayed altered HPA responses to stress: POMC1 (ACTH) levels rose more rapidly in homozygous mutant mice following 2 and 5 min of restraint stress, and declined by 10 min compared with wild-type levels which continued to rise after 10 min

• mutant mice displayed higher corticosterone levels following 10-min restraint, reflecting more robust increases in POMC1 (ACTH) levels at early time points

• mutants showed abnormal recovery from HPA activation: they displayed significantly higher corticosterone levels (90 min post-stress) compared with wild-type mice

• a modest increase in urocortin expression in the Edinger-Westphal nucleus was revealed in mutant mice by in situ hybridization

immune system

immune system phenotype ( J:61510 )

N • homozygous mutant mice displayed normal immune composition