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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
F3tm1Dco
targeted mutation 1, Desire Collen
MGI:2178434
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
F3tm1Dco/F3tm1Dco Not Specified MGI:3687345
cx2
F3tm1Dco/F3tm1Dco
Tg(F3)1Nmk/0
B6.Cg-F3tm1Dco Tg(F3)1Nmk MGI:4822465
cx3
F3tm1Dco/F3tm1Dco
Thbdtm2Rdr/Thbdtm2Rdr
Tg(F3)1Nmk/0
involves: 129/Sv * 129S2/SvPas * BALB/c * C57BL/6 MGI:3687574
cx4
F3tm1Dco/F3tm1Dco
Tg(F3)1Nmk/0
involves: 129/Sv * BALB/c * C57BL/6 MGI:2654490
cx5
F3tm1Dco/F3tm1Dco
Thbdtm2Rdr/Thbdtm2Rdr
involves: 129S2/SvPas * C57BL/6 MGI:2653104
cx6
F3tm1Dco/F3tm1Dco
Procrtm1Cte/Procrtm1Cte
Tg(F3)1Nmk/0
involves: 129S7/SvEvBrd * BALB/c * Black Swiss * C57BL/6 * FVB/N MGI:3702952
cx7
F3tm1Dco/F3tm1Dco
Procrtm1Cte/Procrtm1Cte
involves: 129S7/SvEvBrd * BALB/c * Black Swiss * C57BL/6 * FVB/N MGI:3702956
cx8
F3tm1Dco/F3tm1Dco
Tg(F3)1Nmk/?
involves: BALB/c * C57BL/6 MGI:3826856


Genotype
MGI:3687345
hm1
Allelic
Composition
F3tm1Dco/F3tm1Dco
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygotes die in utero between E9.5 and E10.5
• a small number (1.3%) survive beyond E10.5 but die at ~E13.5-E14.5 of unknown causes

embryo
• at E9.5, ~33.3% of mutant embryos are normal; however, nearly all embryos appear abnormal at E10.5, with extensive necrosis both in the embryo proper and yolk sac
• at E9.5, severely affected homozygotes display extensive embryonic tissue necrosis
• at E9.5, homozygotes exhibit wasting of variable onset and penetrance
• ensuing necrosis appears to result from ischemia secondary to defective vitello-embryonic circulation and wasting
• severely affected homozygotes display extensive embryonic tissue necrosis
• at E9.5, ~66.6% of homozygotes display abnormal yolk sac vessels with strands of acellular material connecting endoderm and mesothelium
• vascular yolk-sac defects precede embryo wasting and necrosis and are noted in embryos still embedded in their decidua
• at E9.5, fragility of mutant vitelline vessels is associated with lack of mesenchymal cell/pericyte accumulation and function, reduced extracellular matrix, and hypoplasia of the muscular wall of primitive vessels; in contrast, endoderm, endothelial and mesothelial cells appear normal
• at E9.5, ~66.6% of mutant yolk sacs lack large vitelline vessels
• at E9.5, ~66.6% of homozygotes exhibit an irregular vascular plexus of enlarged capillaries that appear to fuse with each other
• in vitro, cultured E9.5 mutant embryos exhibit a confluent disorganized vascular plexus ("blood lakes") with no large vitelline vessels
• at E9.5, no blood flow is observed in mutant yolk sacs
• in vitro, cultured E9.5 mutant embryos exhibit pale yolk sacs

cardiovascular system
• at E9.5, ~66.6% of homozygotes display abnormal yolk sac vessels with strands of acellular material connecting endoderm and mesothelium
• vascular yolk-sac defects precede embryo wasting and necrosis and are noted in embryos still embedded in their decidua
• at E9.5, fragility of mutant vitelline vessels is associated with lack of mesenchymal cell/pericyte accumulation and function, reduced extracellular matrix, and hypoplasia of the muscular wall of primitive vessels; in contrast, endoderm, endothelial and mesothelial cells appear normal
• at E9.5, ~66.6% of mutant yolk sacs lack large vitelline vessels
• at E9.5, ~66.6% of homozygotes exhibit an irregular vascular plexus of enlarged capillaries that appear to fuse with each other
• in vitro, cultured E9.5 mutant embryos exhibit a confluent disorganized vascular plexus ("blood lakes") with no large vitelline vessels
• in vitro, severely affected E9.5 mutant embryos exhibit an enlarged pericardial sac and blood in the extracoelomic cavity

integument
• at E9.5, severely affected homozygotes appear pale and anemic due to extravasation of embryonic blood cells in the extracoelomic cavity




Genotype
MGI:4822465
cx2
Allelic
Composition
F3tm1Dco/F3tm1Dco
Tg(F3)1Nmk/0
Genetic
Background
B6.Cg-F3tm1Dco Tg(F3)1Nmk
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
Tg(F3)1Nmk mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• when challenged with lipopolysaccharide (LPS), mutants exhibit prolonged survival and decreased mortality compared to controls

homeostasis/metabolism
• LPS induced formation of thrombin-antithrombin-III complexes is reduced compared to controls, indicating that activation of coagulation is attenuated
• IL-6 expression is reduced at 8-12 hours after LPS challenge

immune system
• IL-6 expression is reduced at 8-12 hours after LPS challenge
• when challenged with lipopolysaccharide (LPS), mutants exhibit prolonged survival and decreased mortality compared to controls




Genotype
MGI:3687574
cx3
Allelic
Composition
F3tm1Dco/F3tm1Dco
Thbdtm2Rdr/Thbdtm2Rdr
Tg(F3)1Nmk/0
Genetic
Background
involves: 129/Sv * 129S2/SvPas * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
Tg(F3)1Nmk mutation (0 available)
Thbdtm2Rdr mutation (0 available); any Thbd mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• mutant embryos develop normally beyond E10.5 and are viable to birth

cardiovascular system
• at E10.5, mutant embryos exhibit bleeding in the placental labyrinth, not caused by thrombomodulin deficiency but characteristic of embryos with low F3 activity




Genotype
MGI:2654490
cx4
Allelic
Composition
F3tm1Dco/F3tm1Dco
Tg(F3)1Nmk/0
Genetic
Background
involves: 129/Sv * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
Tg(F3)1Nmk mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• unlike homozygous null embryos, rescued mice carrying the human minigene and expressing low (<1%) of activity survive embryonic lethality and display normal development and fertility, with no signs of a bleeding diathesis and normal viability up to 7 months of age




Genotype
MGI:2653104
cx5
Allelic
Composition
F3tm1Dco/F3tm1Dco
Thbdtm2Rdr/Thbdtm2Rdr
Genetic
Background
involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
Thbdtm2Rdr mutation (0 available); any Thbd mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• unlike Thbdtm2Rdr homozygotes which are completely resorbed by E9.5, double homozygotes develop normally until E9.5 but become necrotic by E10.5 due to defective yolk sac vasculature

embryo
• by E10.5, double homozygotes display defective vitelline vasculature with free blood pools in the yolk sac cavity
• by E10.5, double mutant yolk sacs lack blood-filled larger vitelline vessels

cardiovascular system
• by E10.5, double homozygotes display defective vitelline vasculature with free blood pools in the yolk sac cavity
• by E10.5, double mutant yolk sacs lack blood-filled larger vitelline vessels
• by E10.5, double homozygotes exhibit an enlarged pericardial cavity, similar to F3tm1Dco homozygotes




Genotype
MGI:3702952
cx6
Allelic
Composition
F3tm1Dco/F3tm1Dco
Procrtm1Cte/Procrtm1Cte
Tg(F3)1Nmk/0
Genetic
Background
involves: 129S7/SvEvBrd * BALB/c * Black Swiss * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
Procrtm1Cte mutation (0 available); any Procr mutation (6 available)
Tg(F3)1Nmk mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• mice are born at expected frequency and survive at least 8 months




Genotype
MGI:3702956
cx7
Allelic
Composition
F3tm1Dco/F3tm1Dco
Procrtm1Cte/Procrtm1Cte
Genetic
Background
involves: 129S7/SvEvBrd * BALB/c * Black Swiss * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
Procrtm1Cte mutation (0 available); any Procr mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no embryos with this genotype are found at birth




Genotype
MGI:3826856
cx8
Allelic
Composition
F3tm1Dco/F3tm1Dco
Tg(F3)1Nmk/?
Genetic
Background
involves: BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
F3tm1Dco mutation (0 available); any F3 mutation (11 available)
Tg(F3)1Nmk mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• hemosiderin deposition and fibrosis after 6 months of age





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last database update
12/05/2017
MGI 6.11
The Jackson Laboratory