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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Alpltm1(cre)Nagy
targeted mutation 1, Andras Nagy
MGI:2177588
Summary 15 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Resttm1.1Yasu/Resttm1.2Yasu
Alpltm1(cre)Nagy/Alpl+
involves: 129 * C57BL/6 * C57BL/6J MGI:5450879
cn2
Alpltm1(cre)Nagy/Alpl+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo
involves: 129 * C57BL/6 * C57BL/6JJcl MGI:6887746
cn3
Alpltm1(cre)Nagy/Alpl+
Atg7tm1Tchi/Atg7tm1Tchi
involves: 129 * C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj MGI:6870513
cn4
Alpltm1(cre)Nagy/Alpl+
Tet3tm1.1Gxu/Tet3tm1.2Gxu
involves: 129 * C57BL/6J * SJL MGI:5294565
cn5
Alpltm1(cre)Nagy/Alpl+
Pou5f1tm1Scho/Pou5f1tm1.1Scho
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3579642
cn6
Alpltm1(cre)Nagy/Alpl+
Dicer1tm1.1Mnn/Dicer1tm1.2Mnn
involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J MGI:3850213
cn7
Alpltm1(cre)Nagy/Alpl+
Dnmt3atm3.1Enl/Dnmt3atm3.2Enl
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ MGI:3047840
cn8
Alpltm1(cre)Nagy/Alpl+
Dnmt3btm5.1Enl/Dnmt3btm5.2Enl
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * FVB/N MGI:3047842
cn9
Alpltm1(cre)Nagy/Alpl+
Xrcc1tm1Pmc/Xrcc1tm1Pmc
involves: 129S1/Sv * 129X1/SvJ MGI:6827282
cn10
Snrpntm2.1Kaj/Snrpn+
Alpltm1(cre)Nagy/Alpl+
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:5140868
cn11
Alpltm1(cre)Nagy/Alpl+
Dmrt1tm1Zark/Dmrt1tm1.1Zark
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3851611
cn12
Guf1tm1Nju/Guf1tm1Nju
Alpltm1(cre)Nagy/Alpl+
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:7439374
cn13
Alpltm1(cre)Nagy/Alpl+
Ehmt2tm2Yshk/Ehmt2tm2.1Yshk
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA MGI:3718794
cn14
Alpltm1(cre)Nagy/Alpl+
Phldb2tm1Msat/Phldb2tm1Msat
involves: 129S1/Sv * 129X1/SvJ * C57BL/6JSlc * C57BL/6NCrlj * CBA/JNCrlj MGI:6364813
cn15
Chtf18tm1.1KhK/Chtf18tm1.2Khk
Alpltm1(cre)Nagy/Alpl+
involves: 129S1/Sv * 129X1/SvJ * FVB/N MGI:5473610


Genotype
MGI:5450879
cn1
Allelic
Composition
Resttm1.1Yasu/Resttm1.2Yasu
Alpltm1(cre)Nagy/Alpl+
Genetic
Background
involves: 129 * C57BL/6 * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Resttm1.1Yasu mutation (1 available); any Rest mutation (95 available)
Resttm1.2Yasu mutation (0 available); any Rest mutation (95 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• despite the decrease in primordial germ cell numbers, germ cell numbers at E18.5 and fertility of adult mice are similar to wild-type controls
• at E12.5 in the genital ridge
• increase in the ratio of apoptotic to viable PGCs at E11.5

cellular
• at E12.5 in the genital ridge
• increase in the ratio of apoptotic to viable PGCs at E11.5




Genotype
MGI:6887746
cn2
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo
Genetic
Background
involves: 129 * C57BL/6 * C57BL/6JJcl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Pknox1tm1.1Rygo mutation (1 available); any Pknox1 mutation (90 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• adult testes contain relatively few c-Kit+ cells with a weak level of c-Kit expression relative to control testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
• adult seminiferous tubules lack SCP3+ meiotic cells
• adult epididymides are devoid of sperm
• a few seminiferous tubules show accumulation of TUNEL+ apoptotic cells inside the lumen
• c-Kit+ spermatogonia appear to be PCNA-negative whereas control c-Kit+ spermatogonia express a high level of PCNA, suggesting a possible proliferation defect
• however, GFRalpha1+ cells (most immature spermatogonial cells) are PCNA-negative, similar to control spermatogonia
• at 3 months of age, nearly all seminiferous tubules appear atrophied and show loss of germ cells
• a few tubules exhibit accumulation of TUNEL+ apoptotic cells inside the lumen
• at 3 months of age, testis size is significantly lower than that in controls
• at 3 months of age, testis weight is significantly lower than that in controls
• adult spermatogenesis is arrested at the c-Kit+ spermatogonia stage

cellular
• GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• adult testes contain relatively few c-Kit+ cells with a weak level of c-Kit expression relative to control testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
• adult seminiferous tubules lack SCP3+ meiotic cells
• adult epididymides are devoid of sperm
• a few seminiferous tubules show accumulation of TUNEL+ apoptotic cells inside the lumen
• c-Kit+ spermatogonia appear to be PCNA-negative whereas control c-Kit+ spermatogonia express a high level of PCNA, suggesting a possible proliferation defect
• however, GFRalpha1+ cells (most immature spermatogonial cells) are PCNA-negative, similar to control spermatogonia

endocrine/exocrine glands
• at 3 months of age, nearly all seminiferous tubules appear atrophied and show loss of germ cells
• a few tubules exhibit accumulation of TUNEL+ apoptotic cells inside the lumen
• at 3 months of age, testis size is significantly lower than that in controls
• at 3 months of age, testis weight is significantly lower than that in controls




Genotype
MGI:6870513
cn3
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Atg7tm1Tchi/Atg7tm1Tchi
Genetic
Background
involves: 129 * C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Atg7tm1Tchi mutation (3 available); any Atg7 mutation (50 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• total number of spermatozoa in the cauda epididymis is severely reduced
• acrosomes fail to acquire the characteristic crescent moon shape and display various defects, including mislocalization, deformation and fragmentation
• fragmented acrosomes are observed in the Golgi and cap phases
• acrosome shrinkage is detected in the cap phase due to accumulated proacrosomal vesicles derived from the Golgi apparatus
• vacuolated or irregularly shaped acrosomes are seen in subsequent stages of spermiogenesis
• acrosome biogenesis is disrupted starting in the Golgi phase: multiple small vesicles are localized to the perinuclear region without fusing with each other in ~30% of Golgi-phase spermatids
• in the cap-phase, the acrosome fails to spread normally along the nucleus and many proacrosomal vesicles accumulate in the concave region near the trans-Golgi stacks in ~27% of spermatids
• in the acrosome and maturation phases, irregular or roughly round acrosomes are found in ~24% of spermatids and the nucleus shows less chromatin condensation and/or impaired nuclear elongation
• defect in acrosome formation is most likely due to the failure of Golgi-derived proacrosomal vesicle fusion and/or membrane trafficking
• multiple small vesicles are localized to the perinuclear region without fusing with each other in ~30% of Golgi-phase spermatids
• TEM images show two proacrosomal centers present around the nucleus
• 26.80% of sperm exhibit irregularly shaped round heads, only seen in 0.42% of control sperm
• in the acrosome and maturation phases, the nucleus neighboring malformed acrosomes shows less chromatin condensation and/or impaired nuclear elongation, resulting in round or irregularly shaped nuclei
• TUNEL staining showed a significantly higher % of apoptotic cells in the seminiferous tubules (0.47% versus 0.04% in control tubules)
• seminiferous tubule structure appears disorganized: 36.67% of tubules contain large vacuoles in their lumen versus only 1.33% of control tubules
• TUNEL staining showed a significantly higher % of apoptotic cells in the seminiferous tubules (0.47% versus 0.04% in control tubules)
• however, seminiferous tubule diameter is normal
• testis size is significantly smaller than that in control males
• testis weight is significantly lower than that in control males
• acrosome biogenesis is highly disrupted during early stages of spermiogenesis
• many detached premature germ cells and abnormal spermatozoa are detected in the cauda epididymis
• males are almost completely infertile: when mated to wild-type females for a 2-month period, pregnancy rate is only 8.14% versus 87.01% for control males
• in vivo fertilization capacity of spermatozoa is impaired; when male mice are mated with wild-type females, no 2-cell embryos are obtained
• however, this defect is successfully rescued by intracytoplasmic sperm injections
• in vitro, the rate of A23187-induced acrosome reaction is severely reduced: after induction, only 38.67% of spermatozoa show loss of their PSA-positive structures versus 63.87% in control sperm, suggesting failure to release acrosomal contents
• however, the rate of spontaneous acrosome reaction is relatively normal

cellular
• total number of spermatozoa in the cauda epididymis is severely reduced
• acrosomes fail to acquire the characteristic crescent moon shape and display various defects, including mislocalization, deformation and fragmentation
• fragmented acrosomes are observed in the Golgi and cap phases
• acrosome shrinkage is detected in the cap phase due to accumulated proacrosomal vesicles derived from the Golgi apparatus
• vacuolated or irregularly shaped acrosomes are seen in subsequent stages of spermiogenesis
• acrosome biogenesis is disrupted starting in the Golgi phase: multiple small vesicles are localized to the perinuclear region without fusing with each other in ~30% of Golgi-phase spermatids
• in the cap-phase, the acrosome fails to spread normally along the nucleus and many proacrosomal vesicles accumulate in the concave region near the trans-Golgi stacks in ~27% of spermatids
• in the acrosome and maturation phases, irregular or roughly round acrosomes are found in ~24% of spermatids and the nucleus shows less chromatin condensation and/or impaired nuclear elongation
• defect in acrosome formation is most likely due to the failure of Golgi-derived proacrosomal vesicle fusion and/or membrane trafficking
• multiple small vesicles are localized to the perinuclear region without fusing with each other in ~30% of Golgi-phase spermatids
• TEM images show two proacrosomal centers present around the nucleus
• 26.80% of sperm exhibit irregularly shaped round heads, only seen in 0.42% of control sperm
• in the acrosome and maturation phases, the nucleus neighboring malformed acrosomes shows less chromatin condensation and/or impaired nuclear elongation, resulting in round or irregularly shaped nuclei
• autophagic flux is disrupted in the testis, as indicated by a 1.5-fold increase in polyubiquitinated protein level and accumulation of the autophagic substrate SQSTM1/p62 and of LC3-I but not the membrane-associated form LC3-II
• TUNEL staining showed a significantly higher % of apoptotic cells in the seminiferous tubules (0.47% versus 0.04% in control tubules)

homeostasis/metabolism
• autophagic flux is disrupted in the testis, as indicated by a 1.5-fold increase in polyubiquitinated protein level and accumulation of the autophagic substrate SQSTM1/p62 and of LC3-I but not the membrane-associated form LC3-II

endocrine/exocrine glands
• seminiferous tubule structure appears disorganized: 36.67% of tubules contain large vacuoles in their lumen versus only 1.33% of control tubules
• TUNEL staining showed a significantly higher % of apoptotic cells in the seminiferous tubules (0.47% versus 0.04% in control tubules)
• however, seminiferous tubule diameter is normal
• testis size is significantly smaller than that in control males
• testis weight is significantly lower than that in control males




Genotype
MGI:5294565
cn4
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Tet3tm1.1Gxu/Tet3tm1.2Gxu
Genetic
Background
involves: 129 * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Tet3tm1.1Gxu mutation (0 available); any Tet3 mutation (59 available)
Tet3tm1.2Gxu mutation (0 available); any Tet3 mutation (59 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• decrease in frequency of successful pregnancy per mating and litter size when crossed to wild-type males

cellular
• paternal genome reprogramming is impaired in offspring of mutant females
• paternal genome reprogramming is impaired in offspring of mutant females
• inheriting the mutant allele maternally impairs embryonic survival




Genotype
MGI:3579642
cn5
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Pou5f1tm1Scho/Pou5f1tm1.1Scho
Genetic
Background
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Pou5f1tm1.1Scho mutation (0 available); any Pou5f1 mutation (82 available)
Pou5f1tm1Scho mutation (1 available); any Pou5f1 mutation (82 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• variable depletion of spermatogenic cells is seen with testes containing both germ cell free seminiferous tubules and unaffected tubules
• the percentage of germ cell free tubules varied from 30% to 100%; however unlike females gem cell depletion did no increase with age
• at E10.5 and E12.5 the late migratory and postmigratory primordial germ cell populations are reduced; however the size of the premigratory (E6.5) and early migratory (E9.5) primordial germ cell populations are normal
• up to 70% of primordial germ cells undergo apoptosis before colonizing the gonadal ridges

reproductive system
• variable depletion of spermatogenic cells is seen with testes containing both germ cell free seminiferous tubules and unaffected tubules
• the percentage of germ cell free tubules varied from 30% to 100%; however unlike females gem cell depletion did no increase with age
• at E10.5 and E12.5 the late migratory and postmigratory primordial germ cell populations are reduced; however the size of the premigratory (E6.5) and early migratory (E9.5) primordial germ cell populations are normal
• up to 70% of primordial germ cells undergo apoptosis before colonizing the gonadal ridges
• at 3 weeks of age the number of primordial follicles is reduced 25 - 100-fold
• at 6 weeks of age hardly any primordial follicles and very few growing follicles are seen
• after 6 weeks of age ovarian follicles are mostly absent
• seminiferous tubules lacking germ cells are reduced in diameter
• seen around seminiferous tubules lacking germ cells
• fertility is variably impaired depending on the efficiency of Cre recombination (averaging about 60%)
• males that do produce offspring pass on the unrecombined allele

endocrine/exocrine glands
• at 3 weeks of age the number of primordial follicles is reduced 25 - 100-fold
• at 6 weeks of age hardly any primordial follicles and very few growing follicles are seen
• after 6 weeks of age ovarian follicles are mostly absent
• seminiferous tubules lacking germ cells are reduced in diameter
• seen around seminiferous tubules lacking germ cells

embryo
• clusters of apoptotic primordial germ cells are detected on the genital ridge at E10.5




Genotype
MGI:3850213
cn6
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Dicer1tm1.1Mnn/Dicer1tm1.2Mnn
Genetic
Background
involves: 129S1/Sv * 129S1/SvImJ * 129X1/SvJ * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Dicer1tm1.1Mnn mutation (0 available); any Dicer1 mutation (94 available)
Dicer1tm1.2Mnn mutation (0 available); any Dicer1 mutation (94 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• half of the ovulated oocytes exhibit defects in spindles and have scattered chromosomes unlike in wild-type oocytes
• half of the ovulated oocytes exhibit defects in spindles

cellular
• half of the ovulated oocytes exhibit defects in spindles and have scattered chromosomes unlike in wild-type oocytes




Genotype
MGI:3047840
cn7
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Dnmt3atm3.1Enl/Dnmt3atm3.2Enl
Genetic
Background
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Dnmt3atm3.1Enl mutation (2 available); any Dnmt3a mutation (138 available)
Dnmt3atm3.2Enl mutation (0 available); any Dnmt3a mutation (138 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• testes weight is greatly reduced at 11 weeks of age

reproductive system
• at P11 the number of spermatogonia are reduced and by 11 weeks of age few spermatogonia and no spermatocytes, spermatids, or spermatozoa are seen
• testes weight is greatly reduced at 11 weeks of age
• all embryos die around E10.5 with open neural tubes, absent branchial arches and pale skin
• this is a maternal effect mutation resulting from impaired maternal imprinting
• no pregnancies result from crossing mutant males with wild-type females

cellular
• at P11 the number of spermatogonia are reduced and by 11 weeks of age few spermatogonia and no spermatocytes, spermatids, or spermatozoa are seen




Genotype
MGI:3047842
cn8
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Dnmt3btm5.1Enl/Dnmt3btm5.2Enl
Genetic
Background
involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Dnmt3btm5.1Enl mutation (3 available); any Dnmt3b mutation (50 available)
Dnmt3btm5.2Enl mutation (0 available); any Dnmt3b mutation (50 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• crossing mutant males or females with wild-type mice results in the generation of litters of healthy pups




Genotype
MGI:6827282
cn9
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Xrcc1tm1Pmc/Xrcc1tm1Pmc
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Xrcc1tm1Pmc mutation (1 available); any Xrcc1 mutation (45 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• increased expression of DNA damage marker gamma-H2AX in spermatogonia at 8 weeks of age
• decreased expression of self-differentiation marker KIT and self-renewal marker ZBTB16 (zinc finger and BTB domain containing 16, also known as PLZF) in testicular tissues, indicating loss of stemness in spermatogonial stem cells
• up-regulated expression of apoptotic mitochondrial genes and increased TUNEL+ spermatogenic cells detected in testes, along with increased BAX expression, decreased BCL2 expression, and higher levels of the cleaved forms of caspase-3 and caspase-9
• decreased sperm motility at 8 weeks of age
• markedly reduced cell counts per seminiferous tubule at 8 weeks of age
• missing layers and epithelial disorganization in seminiferous tubules at 8 weeks of age
• severely reduced testis size at 8 weeks of age
• severe reduction in testis weight at 8 weeks of age
• however, body weight is normal
• decreased expression of spermatogenesis markers Stra8 (stimulated by retinoic acid gene 8) and Sycp3 (synaptonemal complex protein 3) in testes, indicating impaired spermatogenesis
• decreased sperm concentration at 8 weeks of age
• severe nuclear condensation and mitochondrial vacuolization in spermatocytes from 8-wk-old mice
• male mice mated with wild-type female mice fail to produce offspring

cellular
• decreased sperm concentration at 8 weeks of age
• severe nuclear condensation and mitochondrial vacuolization in spermatocytes from 8-wk-old mice
• increased expression of DNA damage marker gamma-H2AX in spermatogonia at 8 weeks of age
• decreased expression of self-differentiation marker KIT and self-renewal marker ZBTB16 (zinc finger and BTB domain containing 16, also known as PLZF) in testicular tissues, indicating loss of stemness in spermatogonial stem cells
• severe mitochondrial vacuolization in spermatocytes from 8-wk-old mice
• however, number of mitochondria is normal
• up-regulated expression of apoptotic mitochondrial genes and increased TUNEL+ spermatogenic cells detected in testes, along with increased BAX expression, decreased BCL2 expression, and higher levels of the cleaved forms of caspase-3 and caspase-9
• decreased sperm motility at 8 weeks of age
• marked reduction in mitochondrial membrane potential in testicular cells, indicating mitochondria dysfunction
• marked decrease in transcriptional levels of mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 2 (Nd2), -4, -5, -6 (complex I) and cytochrome b (complex III), along with significant reduction in complex I and complex III activities and ATP levels in testicular tissues
• reduction in glutathione to glutathione disulfide (GSH/GSSG) ratio, activity of superoxide dismutase, and catalase activity in testes at 8 weeks of age
• increase in malondialdehyde level and expression of DNA damage marker gamma-H2AX in testes at 8 weeks of age

endocrine/exocrine glands
• up-regulated expression of apoptotic mitochondrial genes and increased TUNEL+ spermatogenic cells detected in testes, along with increased BAX expression, decreased BCL2 expression, and higher levels of the cleaved forms of caspase-3 and caspase-9
• markedly reduced cell counts per seminiferous tubule at 8 weeks of age
• missing layers and epithelial disorganization in seminiferous tubules at 8 weeks of age
• severely reduced testis size at 8 weeks of age
• severe reduction in testis weight at 8 weeks of age
• however, body weight is normal

homeostasis/metabolism
• reduction in catalase activity in testes at 8 weeks of age




Genotype
MGI:5140868
cn10
Allelic
Composition
Snrpntm2.1Kaj/Snrpn+
Alpltm1(cre)Nagy/Alpl+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Snrpntm2.1Kaj mutation (1 available); any Snrpn mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• when the floxed allele is paternally inherited, only one mouse survived beyond P2 to die at P7
• however, mice that inherit the floxed allele maternally exhibit normal survival

growth/size/body
• when the floxed allele is paternally inherited

cellular
• when the floxed allele is paternally inherited, DNA methylation at Mkrn3 and Ndn is increased compared to in wild-type mice

behavior/neurological
• when the floxed allele is paternally inherited




Genotype
MGI:3851611
cn11
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Dmrt1tm1Zark/Dmrt1tm1.1Zark
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Dmrt1tm1.1Zark mutation (0 available); any Dmrt1 mutation (48 available)
Dmrt1tm1Zark mutation (0 available); any Dmrt1 mutation (48 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• many germ cells fail to migrate to the periphery
• fewer germ cells indicating cells fail to reactivate mitosis
• many germ cells fail to migrate to the periphery
• however, Sertoli cells are normally polarized

endocrine/exocrine glands
• many germ cells fail to migrate to the periphery
• however, Sertoli cells are normally polarized

cellular
• many germ cells fail to migrate to the periphery
• fewer germ cells indicating cells fail to reactivate mitosis




Genotype
MGI:7439374
cn12
Allelic
Composition
Guf1tm1Nju/Guf1tm1Nju
Alpltm1(cre)Nagy/Alpl+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Guf1tm1Nju mutation (0 available); any Guf1 mutation (43 available)
phenotype observed in females
phenotype observed in males
N normal phenotype



Genotype
MGI:3718794
cn13
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Ehmt2tm2Yshk/Ehmt2tm2.1Yshk
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Ehmt2tm2.1Yshk mutation (0 available); any Ehmt2 mutation (55 available)
Ehmt2tm2Yshk mutation (1 available); any Ehmt2 mutation (55 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• in adults
• germ cell numbers are reduced by about 20% at E16.5 and by about 90% from E17.5 onwards
• very few primordial oocytes are present at P0
• numbers of maturing and primordial oocytes are greatly reduced in adults
• about 70% of seminiferous tubules lack all germ cells while the remainder have only a few spermatogonia and leptotene-like cells
• meiosis is normal until the zygotene stage however very few cells progress to the pachytene stage
• about 20% of pachytene oocytes display perturbed synaptic progression
• arrest of meiosis in the early pachytene stage with incomplete synaptonemal complex formation
• all females older than 7 months of age were infertile
• prior to 7 months of age 19 of 22 females were infertile
• the few fertile females produce smaller litters with some progeny expressing the floxed allele indicating incomplete Cre-mediated recombinatio
• all males are infertile

endocrine/exocrine glands
• in adults

cellular
• germ cell numbers are reduced by about 20% at E16.5 and by about 90% from E17.5 onwards
• very few primordial oocytes are present at P0
• numbers of maturing and primordial oocytes are greatly reduced in adults
• about 70% of seminiferous tubules lack all germ cells while the remainder have only a few spermatogonia and leptotene-like cells




Genotype
MGI:6364813
cn14
Allelic
Composition
Alpltm1(cre)Nagy/Alpl+
Phldb2tm1Msat/Phldb2tm1Msat
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6JSlc * C57BL/6NCrlj * CBA/JNCrlj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Phldb2tm1Msat mutation (0 available); any Phldb2 mutation (66 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• eccentrically shifted PSD-95 localization toward the dendritic shaft
• reduced NMDA and GluA2 receptor accumulation in dendritic spines
• however, postpynaptic density areas are normal
• induced by high-frequency stimulation
• however, basal synaptic function is normal

behavior/neurological
N
• mice exhibit normal locomotor activity and movement in the home cage
• in a T-maze, mice exhibit fewer correct choices compared with wild-type mice
• however, duration time and total distances are normal




Genotype
MGI:5473610
cn15
Allelic
Composition
Chtf18tm1.1KhK/Chtf18tm1.2Khk
Alpltm1(cre)Nagy/Alpl+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (351 available)
Chtf18tm1.1KhK mutation (0 available); any Chtf18 mutation (34 available)
Chtf18tm1.2Khk mutation (0 available); any Chtf18 mutation (34 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• abnormal spermatogenic cells as in Chtf18tm1.1Khk homozygotes

cellular
• abnormal spermatogenic cells as in Chtf18tm1.1Khk homozygotes





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory