Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk8tm1Flv mutation
(3 available);
any
Mapk8 mutation
(71 available)
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Abnormal epidermis of adult Mapk8tm1Flv/Mapk8tm1Flv and Mapk9tm1Flv/Mapk9tm1Flv mice
integument
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• number of proliferating cells in the stratum basale is reduced
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• keratohyalin granules, markers of epidermal differentiation, are missing
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• epidermis is thin and consists of fewer cell layers
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk8tm1Flv mutation
(3 available);
any
Mapk8 mutation
(71 available)
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liver/biliary system
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• exhibit similar liver injury and mortality from toxin-induced (galactosamine/lipopolysaccharide) liver damage as wild-type
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk8tm1Flv mutation
(3 available);
any
Mapk8 mutation
(71 available)
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immune system
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• splenocytes and CD4+ T cells display enhanced proliferation in response to stimulation and CD4+ helper T cells show a moderate reduction of activation-induced cell death
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• helper T cells preferentially differentiate to T helper 2 cells, whereas wild-type cells become T helper 1 cells
• CD4+ T cells are hyperresponsive to anti-CD3 and produce T helper 2 cytokines even in the absence of costimulation
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• T helper 2 cells secrete greatly increased amounts of T helper 2 cytokines (10x as much IL-4, 5x as much IL-5, and moderate increase in IL-10), indicating enhanced T helper 2 cell response
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hematopoietic system
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• splenocytes and CD4+ T cells display enhanced proliferation in response to stimulation and CD4+ helper T cells show a moderate reduction of activation-induced cell death
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• helper T cells preferentially differentiate to T helper 2 cells, whereas wild-type cells become T helper 1 cells
• CD4+ T cells are hyperresponsive to anti-CD3 and produce T helper 2 cytokines even in the absence of costimulation
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neoplasm
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• exhibit increased susceptibility to TPA-induced skin tumor development compared to wild-type, with increased rate and number of papillomas
• exhibit increased incidence and number of TPA-induced carcinomas, indicating that papillomas have an increased risk of undergoing malignant conversion compared to wild-type
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cardiovascular system
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• exhibit inflammatory lesions that lead to the development of significant fibrosis following pressure overload compared to wild-type
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• exhibit a significant reduction in fractional shortening after 3 and 7 days of pressure overload compared to wild-type, however cardiac function improves over time and is similar to wild-type after 12 weeks of TAC
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• exhibit increased rates of myocyte death following pressure overload compared to wild-type
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• develop cardiac hypertrophy similar to wild-type after pressure overload, however display a reduction in fractional shortening, increased inflammatory infiltrate and fibrosis and increased myocyte death compared to wild-type
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muscle
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• exhibit a significant reduction in fractional shortening after 3 and 7 days of pressure overload compared to wild-type, however cardiac function improves over time and is similar to wild-type after 12 weeks of TAC
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• exhibit increased rates of myocyte death following pressure overload compared to wild-type
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homeostasis/metabolism
cellular
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• exhibit increased rates of myocyte death following pressure overload compared to wild-type
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• splenocytes and CD4+ T cells display enhanced proliferation in response to stimulation and CD4+ helper T cells show a moderate reduction of activation-induced cell death
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapk8tm1Flv mutation
(3 available);
any
Mapk8 mutation
(71 available)
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cardiovascular system
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• after induced ischemia-reperfusion (I-R) injury, mice show significant protection compared to wild-type controls as indicated by reduced viable myocardium area at risk (AAR) versus nonviable infarcted area (IA)
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• significantly less cleavage of caspase-3 (reduced by about 50%) is observed compared to wild-type
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muscle
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• after induced ischemia-reperfusion (I-R) injury, mice show significant protection compared to wild-type controls as indicated by reduced viable myocardium area at risk (AAR) versus nonviable infarcted area (IA)
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• significantly less cleavage of caspase-3 (reduced by about 50%) is observed compared to wild-type
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homeostasis/metabolism
cellular
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• significantly less cleavage of caspase-3 (reduced by about 50%) is observed compared to wild-type
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Mapk8tm1Flv/Mapk8tm1Flv Mapk9tm1Flv/Mapk9+ pups exhibit open eyes at birth
mortality/aging
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• 80% die within 48 hours after birth
(J:83385)
• majority die within 48 hours after birth, although a small number survive to adulthood
(J:93433)
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vision/eye
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• exhibit a number of developmental defects in the eye at E18.5
(J:83385)
• mutants that survive to adulthood have opaque eyes
(J:93433)
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• show severe lens abnormality at E18.5
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• smaller lenses with decreased expression of alpha-, beta-, and gamma-crystallin
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• show retinal coloboma at midgestation
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• eyelids exhibit variable extension across the cornea but no contact with the opposing epithelia
(J:93433)
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respiratory system
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• exhibit immature lungs at P1
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• alveolar septae are thicker and more cellular at P1, indicating immaturity of lung development
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• appear to have difficulty breathing at birth
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digestive/alimentary system
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• tongue epidermis shows a reduction in proliferation at E15.5
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• epithelium of tongue is immature at E18.5, with no fungiform taste papillae
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• exhibit no fungiform taste papillae at E18.5
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• pups exhibit immature intestines; intestines have all the differentiated cell types but show irregular loops and shorter villi
• delay in maturation of villi is apparent at E14.5, soon after the villi start to form
• defects in intestines are more severe in embryos than after birth
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• exhibit epithelial immaturity in the intestines
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• number of periodic acid/Schiff reagent-staining surface mucous cells is reduced
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reproductive system
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• mutants that survive to adulthood are sterile
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renal/urinary system
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• E18.5 kidneys have deformed renal epithelium
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• E18.5 kidneys have deformed nephrons
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• E18.5 kidneys contain enlarged tubules
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craniofacial
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• tongue epidermis shows a reduction in proliferation at E15.5
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• epithelium of tongue is immature at E18.5, with no fungiform taste papillae
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• exhibit no fungiform taste papillae at E18.5
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integument
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• interfollicular epidermal proliferation is reduced in E15.5 skin
• E18.5 skin shows disorganized and immature development of the hair follicles
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• newborns exhibit fewer hair follicles
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• exhibit disorganized and immature development of the epidermis at E18.5
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growth/size/body
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• tongue epidermis shows a reduction in proliferation at E15.5
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• epithelium of tongue is immature at E18.5, with no fungiform taste papillae
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• exhibit no fungiform taste papillae at E18.5
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cardiovascular system
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• develop atherosclerosis similar to that seen in single Apoe homozygotes on a high-cholesterol diet for 14 weeks
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nervous system
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• appear healthy and do not exhibit exencephaly or abnormal apoptosis during brain development
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mortality/aging
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• die between E11 and E12
• some mutants start to show embryonic degeneration that included decreased body size, transparency of embryos, and cardiac dilation at E11.5
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nervous system
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• show reduced apoptosis in the hindbrain neural tube during cephalic neurulation at E9
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• exhibit about 10-fold increase in apoptosis in the forebrain neural epithelium at E10.5
• E11 forebrain shows increased pyknosis
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• exhibit about 10-fold increase in apoptosis in the forebrain neural epithelium at E10.5
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• exhibit hindbrain exencephaly that is already visible at E10.5
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embryo
cardiovascular system
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• cardiac dilation in degenerating embryos at E11.5
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cellular
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• show reduced apoptosis in the hindbrain neural tube during cephalic neurulation at E9
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• exhibit about 10-fold increase in apoptosis in the forebrain neural epithelium at E10.5
• E11 forebrain shows increased pyknosis
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• exhibit about 10-fold increase in apoptosis in the forebrain neural epithelium at E10.5
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cellular
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• treatment of TNF or UV-stimulated MEFs with 1NM-PP1 increases TNF-induced apoptosis and decreases Uv-stimulated apoptosis while such treatment causes no change in wild-type or Mapk9tm1Rjd MEFs
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• a scratch wound in monolayers of wild-type MEFs is rapid, but is markedly slowed upon treatment with 1NM-PP1, compared to wild-type or or Mapk9tm1Rjd MEFs
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• keratinocytes in culture show slightly reduced motility compared to wild-type and treatment with 1NM-PP1 results in significantly decreased migration compared to treated or untreated wild-type MEFs
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• compared to wild-type, MEFs show reduced proliferation in culture; after treatment with a protein kinase inhibibitor (1NM-PP1), proliferation is reduced even more
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integument
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• keratinocytes in culture show slightly reduced motility compared to wild-type and treatment with 1NM-PP1 results in significantly decreased migration compared to treated or untreated wild-type MEFs
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Analysis Tools