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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(MMTV-cre)1Mam
transgene insertion 1, Lothar Hennighausen
MGI:2176165
Summary 24 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Tg(MMTV-cre)1Mam/0
Ccn6tm1.1Cgkl/Ccn6tm1.1Cgkl
FVB/N-Ccn6tm1.1Cgkl Tg(MMTV-cre)1Mam MGI:6093470
cn2
Tg(MMTV-cre)1Mam/0
Tg(MMTV-PyVT)634Mul/0
Ccn6tm1.1Cgkl/Ccn6tm1.1Cgkl
FVB/N-Ccn6tm1.1Cgkl Tg(MMTV-cre)1Mam Tg(MMTV-PyVT)634Mul MGI:6093471
cn3
Rev3ltm1Rwd/Rev3ltm2.1Rwd
Trp53tm1Brd/Trp53+
Tg(MMTV-cre)1Mam/0
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * FVB/N MGI:4441276
cn4
Rev3ltm1Rwd/Rev3ltm2.1Rwd
Trp53tm1Brd/Trp53tm1Brd
Tg(MMTV-cre)1Mam/0
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * FVB/N MGI:4441274
cn5
Rev3ltm1Rwd/Rev3ltm2.1Rwd
Tg(MMTV-cre)1Mam/0
involves: 129P2/OlaHsd * C57BL/6 * FVB MGI:4441273
cn6
Rbpjtm1Hon/Rbpjtm1Hon
Tg(MMTV-cre)1Mam/0
involves: 129P2/OlaHsd * C57BL/6J * FVB * SJL MGI:3653205
cn7
Kdm4btm1.1Okad/Kdm4btm1.1Okad
Tg(MMTV-cre)1Mam/0
involves: 129P2/OlaHsd * FVB MGI:4999589
cn8
Pofut1tm2Pst/Pofut1tm2Pst
Tg(MMTV-cre)1Mam/0
involves: 129P2/OlaHsd * FVB MGI:3653206
cn9
Hif1atm3Rsjo/Hif1atm3Rsjo
Tg(MMTV-cre)1Mam/0
involves: 129S1/Sv * 129X1/SvJ * CD-1 * FVB MGI:4418467
cn10
Stat5atm2Mam/Stat5atm2Mam
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
Tg(MMTV-rtTA)1Lach/0
Tg(tetO-Stat5a)#Mam/0
involves: 129S6/SvEvTac * C57BL/6J * FVB/N MGI:4847637
cn11
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
Tg(MMTV-rtTA)1Lach/0
Tg(tetO-Stat5a)#Mam/0
involves: 129S6/SvEvTac * C57BL/6J * FVB/N MGI:4847638
cn12
Stat5atm2Mam/Stat5atm2Mam
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
involves: 129S6/SvEvTac * FVB/N MGI:4847555
cn13
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
involves: 129S6/SvEvTac * FVB/N MGI:4847556
cn14
Gt(ROSA)26Sortm1(Pik3ca*H1047R)Egan/Gt(ROSA)26Sor+
Tg(MMTV-cre)1Mam/0
involves: 129S6/SvEvTac * FVB/N MGI:5000476
cn15
Gt(ROSA)26Sortm2(Pik3ca)Egan/Gt(ROSA)26Sor+
Tg(MMTV-cre)1Mam/0
involves: 129S6/SvEvTac * FVB/N MGI:5000481
cn16
Jak2tm1Kuw/Jak2tm1Kuw
Tg(MMTV-cre)1Mam/0
involves: 129X1/SvJ * C57BL/6 * FVB MGI:3045399
cn17
Ppargtm1Gonz/Ppargtm1Gonz
Tg(MMTV-cre)1Mam/?
involves: 129X1/SvJ * FVB MGI:3722293
cn18
Jak1tm1Kuw/Jak1tm1Kuw
Tg(MMTV-cre)1Mam/0
involves: 129X1/SvJ * FVB MGI:5796282
cn19
Arnttm1Gonz/Arnttm1Gonz
Tg(MMTV-cre)1Mam/0
involves: 129X1/SvJ * FVB MGI:3656140
cn20
Id4tm1Vdr/Id4tm1Vdr
Tg(MMTV-cre)1Mam/0
involves: C57BL/6 * FVB/N MGI:5805279
cn21
Id4tm1Vdr/Id4+
Tg(MMTV-cre)1Mam/0
involves: C57BL/6 * FVB/N MGI:5805280
cn22
Pik3catm1Gne/Pik3ca+
Tg(MMTV-cre)1Mam/0
involves: C57BL/6N * FVB/N MGI:5469591
cn23
Becn1tm1Ebr/Becn1+
Tg(MMTV-cre)1Mam/?
involves: FVB MGI:5308737
tg24
Tg(MMTV-cre)1Mam/? involves: FVB MGI:4888636


Genotype
MGI:6093470
cn1
Allelic
Composition
Tg(MMTV-cre)1Mam/0
Ccn6tm1.1Cgkl/Ccn6tm1.1Cgkl
Genetic
Background
FVB/N-Ccn6tm1.1Cgkl Tg(MMTV-cre)1Mam
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccn6tm1.1Cgkl mutation (0 available); any Ccn6 mutation (3 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• 16 week old adult virgin mice show a reduction in mammary gland complexity
• mammary glands exhibit reduced mitosis
• the non-neoplastic mammary gland tissues show a variety of abnormalities including persistent terminal end buds, secretory hyperplasia, and atypical ductal hyperplasia
• abnormal age-related involution in virgin mice, with all mice at 8 months of age showing residual brown adipose tissue in the mammary glands and terminal end buds that persist in 66.7% of these mice
• however, dams are able to nurse litters and glands undergo post-lactational involution
• hypoplastic ductal epithelium with reduced ductal thickness in 16 week old virgin mice
• ductal epithelial cells are flat instead of tall columnar
• pre-pubertal (5 week) and pubertal (8 week) mice exhibit reduced numbers of terminal end buds and of bifurcated terminal end buds
• persistent terminal end buds are seen in the non-neoplastic mammary gland tissues
• atypical ductal hyperplasia is seen in the non-neoplastic mammary gland tissues
• 72.2% of mice develop mammary carcinomas between 10 and 21 months of age, with a mean age of 15.5 months
• mammary carcinomas exhibit mitotically active elongated cells of high histological grade, similar to the spindle subtype of human metaplastic carcinomas
• tumors are highly invasive into the surrounding mammary tissues and skeletal muscle

integument
• 16 week old adult virgin mice show a reduction in mammary gland complexity
• mammary glands exhibit reduced mitosis
• the non-neoplastic mammary gland tissues show a variety of abnormalities including persistent terminal end buds, secretory hyperplasia, and atypical ductal hyperplasia
• abnormal age-related involution in virgin mice, with all mice at 8 months of age showing residual brown adipose tissue in the mammary glands and terminal end buds that persist in 66.7% of these mice
• however, dams are able to nurse litters and glands undergo post-lactational involution
• hypoplastic ductal epithelium with reduced ductal thickness in 16 week old virgin mice
• ductal epithelial cells are flat instead of tall columnar
• pre-pubertal (5 week) and pubertal (8 week) mice exhibit reduced numbers of terminal end buds and of bifurcated terminal end buds
• persistent terminal end buds are seen in the non-neoplastic mammary gland tissues
• atypical ductal hyperplasia is seen in the non-neoplastic mammary gland tissues
• 72.2% of mice develop mammary carcinomas between 10 and 21 months of age, with a mean age of 15.5 months
• mammary carcinomas exhibit mitotically active elongated cells of high histological grade, similar to the spindle subtype of human metaplastic carcinomas
• tumors are highly invasive into the surrounding mammary tissues and skeletal muscle

neoplasm
• 72.2% of mice develop mammary carcinomas between 10 and 21 months of age, with a mean age of 15.5 months
• mammary carcinomas exhibit mitotically active elongated cells of high histological grade, similar to the spindle subtype of human metaplastic carcinomas
• tumors are highly invasive into the surrounding mammary tissues and skeletal muscle
• distant metastasis develop in 46.2% of tumor-bearing mice, mainly to the lungs and in once case the soft tissues of the neck

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
breast metaplastic carcinoma DOID:4680 J:241259




Genotype
MGI:6093471
cn2
Allelic
Composition
Tg(MMTV-cre)1Mam/0
Tg(MMTV-PyVT)634Mul/0
Ccn6tm1.1Cgkl/Ccn6tm1.1Cgkl
Genetic
Background
FVB/N-Ccn6tm1.1Cgkl Tg(MMTV-cre)1Mam Tg(MMTV-PyVT)634Mul
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccn6tm1.1Cgkl mutation (0 available); any Ccn6 mutation (3 available)
Tg(MMTV-cre)1Mam mutation (1 available)
Tg(MMTV-PyVT)634Mul mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• mice develop mammary tumors with no differences in primary tumor volume from control mice expressing Tg(MMTV-PyVT)654Mul and Tg(MMTV-cre)1Mam

integument
• mice develop mammary tumors with no differences in primary tumor volume from control mice expressing Tg(MMTV-PyVT)654Mul and Tg(MMTV-cre)1Mam

neoplasm
• mice develop mammary tumors with no differences in primary tumor volume from control mice expressing Tg(MMTV-PyVT)654Mul and Tg(MMTV-cre)1Mam
• female virgin mice show an increase in the number of lung metastasis compared to control mice expressing Tg(MMTV-PyVT)654Mul and Tg(MMTV-cre)1Mam




Genotype
MGI:4441276
cn3
Allelic
Composition
Rev3ltm1Rwd/Rev3ltm2.1Rwd
Trp53tm1Brd/Trp53+
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rev3ltm1Rwd mutation (0 available); any Rev3l mutation (73 available)
Rev3ltm2.1Rwd mutation (2 available); any Rev3l mutation (73 available)
Tg(MMTV-cre)1Mam mutation (1 available)
Trp53tm1Brd mutation (7 available); any Trp53 mutation (151 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• 11 of 36 mice develop mammary tumors consisting of solid carcinoma, carcinosarcoma, adenosquamous carcinoma, and osteosarcoma
• incidence of mammary tumors is increased while latency to development of tumors is decreased compared with Trp53tm1Brd heterozygotes

integument
• 11 of 36 mice develop mammary tumors consisting of solid carcinoma, carcinosarcoma, adenosquamous carcinoma, and osteosarcoma
• incidence of mammary tumors is increased while latency to development of tumors is decreased compared with Trp53tm1Brd heterozygotes

skeleton
• in the mammary tissue of one mouse

neoplasm
• 11 of 36 mice develop mammary tumors consisting of solid carcinoma, carcinosarcoma, adenosquamous carcinoma, and osteosarcoma
• incidence of mammary tumors is increased while latency to development of tumors is decreased compared with Trp53tm1Brd heterozygotes
• in the mammary tissue of some mice
• in the mammary tissue of one mouse




Genotype
MGI:4441274
cn4
Allelic
Composition
Rev3ltm1Rwd/Rev3ltm2.1Rwd
Trp53tm1Brd/Trp53tm1Brd
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rev3ltm1Rwd mutation (0 available); any Rev3l mutation (73 available)
Rev3ltm2.1Rwd mutation (2 available); any Rev3l mutation (73 available)
Tg(MMTV-cre)1Mam mutation (1 available)
Trp53tm1Brd mutation (7 available); any Trp53 mutation (151 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• in all mice develop thymic lymphomas compared with 70% to 77% of Trp53tm1Brd homozygous control mice
• tumors are frequently oligoclonal
• incidence of thymic lymphomas is increased while latency to development of tumors is decreased compared with Trp53tm1Brd homozygous control mice

mortality/aging
• mean survival is 91 days compared with 134 to 117 days for Trp53tm1Brd homozygous control mice

neoplasm
• in all mice develop thymic lymphomas compared with 70% to 77% of Trp53tm1Brd homozygous control mice
• tumors are frequently oligoclonal
• incidence of thymic lymphomas is increased while latency to development of tumors is decreased compared with Trp53tm1Brd homozygous control mice




Genotype
MGI:4441273
cn5
Allelic
Composition
Rev3ltm1Rwd/Rev3ltm2.1Rwd
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rev3ltm1Rwd mutation (0 available); any Rev3l mutation (73 available)
Rev3ltm2.1Rwd mutation (2 available); any Rev3l mutation (73 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• 6 of 24 mice develop mammary tumors consisting of carcinosarcoma, solid carcinoma, and adenosquamous carcinoma

integument
• 6 of 24 mice develop mammary tumors consisting of carcinosarcoma, solid carcinoma, and adenosquamous carcinoma

neoplasm
• 6 of 24 mice develop mammary tumors consisting of carcinosarcoma, solid carcinoma, and adenosquamous carcinoma
• in the mammary tissue of some mice




Genotype
MGI:3653205
cn6
Allelic
Composition
Rbpjtm1Hon/Rbpjtm1Hon
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6J * FVB * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rbpjtm1Hon mutation (2 available); any Rbpj mutation (171 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• at day 7 of pregnancy immature alveoli consist of round cells with radially positioned nuclei around the central lumen; cells are irregularly arranged with frequent gaps between them; also many of the lumina contained cellular debris
• on day 11, epithelia appears much sparser
• on day 13, clusters of disorganized cells with irregularly- shaped nuclei are seen and lipid droplets in the lumina are rare and proteinaceous material
• at term, an increase in disorganized cell clusters is seen, with the majority of cells having dark irregularly shaped nuclei surrounding small groups of cells with larger round nuclei
• a paucity of true luminal cells is found in mutant mammary glands during pregnancy
• when mutant mammary epithelium is transplanted into athymic nude mice from which the mammary tissue had been cleared for three generations, it is seen that there is restoration of normal ductal architecture after involution

reproductive system
• at day 7 of pregnancy immature alveoli consist of round cells with radially positioned nuclei around the central lumen; cells are irregularly arranged with frequent gaps between them; also many of the lumina contained cellular debris
• on day 11, epithelia appears much sparser
• on day 13, clusters of disorganized cells with irregularly- shaped nuclei are seen and lipid droplets in the lumina are rare and proteinaceous material
• at term, an increase in disorganized cell clusters is seen, with the majority of cells having dark irregularly shaped nuclei surrounding small groups of cells with larger round nuclei
• a paucity of true luminal cells is found in mutant mammary glands during pregnancy
• when mutant mammary epithelium is transplanted into athymic nude mice from which the mammary tissue had been cleared for three generations, it is seen that there is restoration of normal ductal architecture after involution

integument
• at day 7 of pregnancy immature alveoli consist of round cells with radially positioned nuclei around the central lumen; cells are irregularly arranged with frequent gaps between them; also many of the lumina contained cellular debris
• on day 11, epithelia appears much sparser
• on day 13, clusters of disorganized cells with irregularly- shaped nuclei are seen and lipid droplets in the lumina are rare and proteinaceous material
• at term, an increase in disorganized cell clusters is seen, with the majority of cells having dark irregularly shaped nuclei surrounding small groups of cells with larger round nuclei
• a paucity of true luminal cells is found in mutant mammary glands during pregnancy
• when mutant mammary epithelium is transplanted into athymic nude mice from which the mammary tissue had been cleared for three generations, it is seen that there is restoration of normal ductal architecture after involution
• mutant skin display hyperkeratinization
• cysts containing abundant whorls of keratin are observed




Genotype
MGI:4999589
cn7
Allelic
Composition
Kdm4btm1.1Okad/Kdm4btm1.1Okad
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129P2/OlaHsd * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kdm4btm1.1Okad mutation (0 available); any Kdm4b mutation (35 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Defective mammary gland development in Kdm4btm1.1Okad/Kdm4btm1.1Okad Tg(MMTV-cre)1Mam/0 mice

integument
• mammary gland development is delayed with reduced branching compared to in wild-type mice
• mammary gland branching is reduced compared to in wild-type mice

endocrine/exocrine glands
• mammary gland development is delayed with reduced branching compared to in wild-type mice
• mammary gland branching is reduced compared to in wild-type mice




Genotype
MGI:3653206
cn8
Allelic
Composition
Pofut1tm2Pst/Pofut1tm2Pst
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129P2/OlaHsd * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pofut1tm2Pst mutation (0 available); any Pofut1 mutation (19 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• in transplant experiments, development lesions with respect to disorganized alveolar morphology seen in transplanted mammary tissue are indistinguishable from those observed in Rbsuhtm1Hon conditional mice
• epithelial compartment consists of densely packed cells with irregular dark nuclei

reproductive system
• in transplant experiments, development lesions with respect to disorganized alveolar morphology seen in transplanted mammary tissue are indistinguishable from those observed in Rbsuhtm1Hon conditional mice
• epithelial compartment consists of densely packed cells with irregular dark nuclei

integument
• in transplant experiments, development lesions with respect to disorganized alveolar morphology seen in transplanted mammary tissue are indistinguishable from those observed in Rbsuhtm1Hon conditional mice
• epithelial compartment consists of densely packed cells with irregular dark nuclei
• mutant skin display hyperkeratinization
• cysts containing abundant whorls of keratin are observed




Genotype
MGI:4418467
cn9
Allelic
Composition
Hif1atm3Rsjo/Hif1atm3Rsjo
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * CD-1 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hif1atm3Rsjo mutation (3 available); any Hif1a mutation (11 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• at day 1 of lactation, mammary glands have fewer alveoli, fewer milk granules, and increased trapping of lipid droplets within epithelial cells compared to in wild-type mice
• during lactation, mammary gland wet weight is decreased 50% compared to in wild-type mice
• by day 15 of pregnancy, mammary gland epithelium lack the lacy appearance of wild-type epithelium
• during lactation, epithelial cells trap milk and fat unlike in wild-type mice
• transplanted mammary epithelium fails to grow in mice expressing the wild-type protein
• by day 15 of pregnancy, alveoli are smaller than normal with more prominent surrounding connective tissue than in wild-type mice
• alveolar differentiation during pregnancy is blocked
• alveolar lumens are small compared to in wild-type mice and fail to secret milk
• however, cell proliferation is normal
• female mice fail to secrete sufficient milk to support their offspring, most of whom are runted and die by P15
• milk water content is decreased while milk sodium and chloride ion contents are increased compared to in wild-type mice

cardiovascular system
N
• microvessel patterning and vascular density in the mammary gland are normal

integument
• at day 1 of lactation, mammary glands have fewer alveoli, fewer milk granules, and increased trapping of lipid droplets within epithelial cells compared to in wild-type mice
• during lactation, mammary gland wet weight is decreased 50% compared to in wild-type mice
• by day 15 of pregnancy, mammary gland epithelium lack the lacy appearance of wild-type epithelium
• during lactation, epithelial cells trap milk and fat unlike in wild-type mice
• transplanted mammary epithelium fails to grow in mice expressing the wild-type protein
• by day 15 of pregnancy, alveoli are smaller than normal with more prominent surrounding connective tissue than in wild-type mice
• alveolar differentiation during pregnancy is blocked
• alveolar lumens are small compared to in wild-type mice and fail to secret milk
• however, cell proliferation is normal
• female mice fail to secrete sufficient milk to support their offspring, most of whom are runted and die by P15
• milk water content is decreased while milk sodium and chloride ion contents are increased compared to in wild-type mice




Genotype
MGI:4847637
cn10
Allelic
Composition
Stat5atm2Mam/Stat5atm2Mam
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
Tg(MMTV-rtTA)1Lach/0
Tg(tetO-Stat5a)#Mam/0
Genetic
Background
involves: 129S6/SvEvTac * C57BL/6J * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat5atm2Mam mutation (1 available); any Stat5a mutation (7 available)
Stat5btm1Mam mutation (0 available); any Stat5b mutation (24 available)
Tg(MMTV-cre)1Mam mutation (1 available)
Tg(MMTV-rtTA)1Lach mutation (0 available)
Tg(tetO-Stat5a)#Mam mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
N
• with doxycycline food during pregnancy female mice develop functional mammary glands during pregnancy and are able to feed their pups
• expanded alveoli lined with differentiated secretory cells, with proteinaceous material and lipid droplets in the lumen after doxycycline treatment
• formation of alveolar structures and functional differentiation of luminal cells after doxycycline treatment
• the mutant mice generate CD61+ luminal progenitor cells during pregnancy to a similar extent as Stat5a/Stat5btm2Mam control mice

reproductive system
N
• with doxycycline food during pregnancy female mice develop functional mammary glands during pregnancy and are able to feed their pups
• expanded alveoli lined with differentiated secretory cells, with proteinaceous material and lipid droplets in the lumen after doxycycline treatment
• formation of alveolar structures and functional differentiation of luminal cells after doxycycline treatment
• the mutant mice generate CD61+ luminal progenitor cells during pregnancy to a similar extent as Stat5a/Stat5btm2Mam control mice




Genotype
MGI:4847638
cn11
Allelic
Composition
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
Tg(MMTV-rtTA)1Lach/0
Tg(tetO-Stat5a)#Mam/0
Genetic
Background
involves: 129S6/SvEvTac * C57BL/6J * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat5btm1Mam mutation (0 available); any Stat5b mutation (24 available)
Tg(MMTV-cre)1Mam mutation (1 available)
Tg(MMTV-rtTA)1Lach mutation (0 available)
Tg(tetO-Stat5a)#Mam mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
N
• with doxycycline food during pregnancy female mice develop functional mammary glands during pregnancy and are able to feed their pups
• expanded alveoli lined with differentiated secretory cells, with proteinaceous material and lipid droplets in the lumen after doxycycline treatment
• formation of alveolar structures and functional differentiation of luminal cells after doxycycline treatment
• the mutant mice generate CD61+ luminal progenitor cells during pregnancy to a similar extent as Stat5a/Stat5btm2Mam control mice

reproductive system
N
• with doxycycline food during pregnancy female mice develop functional mammary glands during pregnancy and are able to feed their pups
• expanded alveoli lined with differentiated secretory cells, with proteinaceous material and lipid droplets in the lumen after doxycycline treatment
• formation of alveolar structures and functional differentiation of luminal cells after doxycycline treatment
• the mutant mice generate CD61+ luminal progenitor cells during pregnancy to a similar extent as Stat5a/Stat5btm2Mam control mice




Genotype
MGI:4847555
cn12
Allelic
Composition
Stat5atm2Mam/Stat5atm2Mam
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat5atm2Mam mutation (1 available); any Stat5a mutation (7 available)
Stat5btm1Mam mutation (0 available); any Stat5b mutation (24 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• profoundly reduced amount of luminal progenitors (CD61+) in the luminal cell population (CD24hiCD49flo) in mammary tissue of 12 week-old virgin mice and on day 6 of pregnancy
• fail to lactate even after five to six pregnancies

integument
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• profoundly reduced amount of luminal progenitors (CD61+) in the luminal cell population (CD24hiCD49flo) in mammary tissue of 12 week-old virgin mice and on day 6 of pregnancy
• fail to lactate even after five to six pregnancies




Genotype
MGI:4847556
cn13
Allelic
Composition
Stat5btm1Mam/Stat5btm1Mam
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat5btm1Mam mutation (0 available); any Stat5b mutation (24 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• profoundly reduced amount of luminal progenitors (CD61+) in the luminal cell population (CD24hiCD49flo) in mammary tissue of 12 week-old virgin mice and on day 6 of pregnancy
• fail to lactate even after five to six pregnancies

integument
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• the epithelial compartment in stem cell-transplanted hosts fail to develop a full alveolar compartment and devoid of milk in the epithelial lumina at term when mated to induce pregnancy
• produce only a ductal tree with virgin-like characteristics
• profoundly reduced amount of luminal progenitors (CD61+) in the luminal cell population (CD24hiCD49flo) in mammary tissue of 12 week-old virgin mice and on day 6 of pregnancy
• fail to lactate even after five to six pregnancies




Genotype
MGI:5000476
cn14
Allelic
Composition
Gt(ROSA)26Sortm1(Pik3ca*H1047R)Egan/Gt(ROSA)26Sor+
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(Pik3ca*H1047R)Egan mutation (2 available); any Gt(ROSA)26Sor mutation (504 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• start to develop by 5 months of age in both virgin and multiparous mice
• 42% of mice have palpable mammary tumors at the time of death

integument
• start to develop by 5 months of age in both virgin and multiparous mice
• 42% of mice have palpable mammary tumors at the time of death

mortality/aging
• survival time is reduced compared to mutant mice carrying Tg(MMTV-cre)4Mam

neoplasm
• develop a more diverse set of tumors compared to mutant mice carrying Tg(MMTV-cre)4Mam
• start to develop by 5 months of age in both virgin and multiparous mice
• 42% of mice have palpable mammary tumors at the time of death




Genotype
MGI:5000481
cn15
Allelic
Composition
Gt(ROSA)26Sortm2(Pik3ca)Egan/Gt(ROSA)26Sor+
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129S6/SvEvTac * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm2(Pik3ca)Egan mutation (0 available); any Gt(ROSA)26Sor mutation (504 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype




Genotype
MGI:3045399
cn16
Allelic
Composition
Jak2tm1Kuw/Jak2tm1Kuw
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129X1/SvJ * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak2tm1Kuw mutation (0 available); any Jak2 mutation (4 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• absence of the lobular units that normally reside on the terminal ducts of the tertiary side branches
• lack of alveolar precursors results in impairment development of secretory lobules
• mammary epithelia did not undergo alveolar specification during pregnancy
• failure of dams to lactate; multiple pregnancies did not restore milk production

reproductive system
• mammary epithelia did not undergo alveolar specification during pregnancy

integument
• absence of the lobular units that normally reside on the terminal ducts of the tertiary side branches
• lack of alveolar precursors results in impairment development of secretory lobules
• mammary epithelia did not undergo alveolar specification during pregnancy
• failure of dams to lactate; multiple pregnancies did not restore milk production




Genotype
MGI:3722293
cn17
Allelic
Composition
Ppargtm1Gonz/Ppargtm1Gonz
Tg(MMTV-cre)1Mam/?
Genetic
Background
involves: 129X1/SvJ * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ppargtm1Gonz mutation (0 available); any Pparg mutation (30 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• alveolar development induced by pregnancy is impaired
• females cannot nurse pups
• one third of females are infertile
• females that can bred have reduced fertility
• females that can bred produce smaller litters

endocrine/exocrine glands
• alveolar development induced by pregnancy is impaired
• females cannot nurse pups

integument
• alveolar development induced by pregnancy is impaired
• females cannot nurse pups




Genotype
MGI:5796282
cn18
Allelic
Composition
Jak1tm1Kuw/Jak1tm1Kuw
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129X1/SvJ * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Jak1tm1Kuw mutation (0 available); any Jak1 mutation (11 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• females exhibit a delay in postlactational mammary gland remodeling starting at 3 days post weaning and these abnormalities are still evident after 5 days of involution
• however, females lactate normally, and show normal ductal elongation and branching morphogenesis, and mammary gland alveologenesis during pregnancy

integument
• females exhibit a delay in postlactational mammary gland remodeling starting at 3 days post weaning and these abnormalities are still evident after 5 days of involution
• however, females lactate normally, and show normal ductal elongation and branching morphogenesis, and mammary gland alveologenesis during pregnancy




Genotype
MGI:3656140
cn19
Allelic
Composition
Arnttm1Gonz/Arnttm1Gonz
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: 129X1/SvJ * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arnttm1Gonz mutation (0 available); any Arnt mutation (15 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• incomplete development of alveoli probably secondary to impaired ovarian function as tissue transplanted into athymic nude mice develops normally
• contain fewer primordial follicles but similar numbers of primary and preantral/antral follicles compared to controls
• however; estrus cycle and the levels of estradiol and progesterone are similar to controls
• fewer primordial follicles compared to controls
• average litter size is 4 +/- 2 pups

endocrine/exocrine glands
• incomplete development of alveoli probably secondary to impaired ovarian function as tissue transplanted into athymic nude mice develops normally
• contain fewer primordial follicles but similar numbers of primary and preantral/antral follicles compared to controls
• however; estrus cycle and the levels of estradiol and progesterone are similar to controls
• fewer primordial follicles compared to controls
• 60% of dams can not support their pups and surviving pups are smaller compared to pups nursed by homozygous dams lacking Cre expression (controls)

integument
• incomplete development of alveoli probably secondary to impaired ovarian function as tissue transplanted into athymic nude mice develops normally
• 60% of dams can not support their pups and surviving pups are smaller compared to pups nursed by homozygous dams lacking Cre expression (controls)




Genotype
MGI:5805279
cn20
Allelic
Composition
Id4tm1Vdr/Id4tm1Vdr
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Id4tm1Vdr mutation (0 available); any Id4 mutation (7 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• mammary glands from 4-week-old virgin mice display ductal elongation defects, as determined by the % of fat pad filled relative to control mice
• although ductal elongation is largely restored by 8 weeks of age, complex branching of the ducts is still lacking
• treatment with exogenous beta-estradiol at proestrus and estrus rescues the ductal elongation defects
• mammary glands from 6-week-old virgin mice show a significant increase in the % of estrogen receptor (ER)-alpha expressing (ERalpha+) and progesterone receptor expressing (PR+) luminal cells relative to those in control mice
• a significantly increased % of ERalpha+ ductal cells is found in the luminal progenitor subset (CD29loCD24+CD49b+) but not in the mature luminal subset (CD29loCD24+CD49b-)
• % of Foxa1 (forkhead box A1) expressing (FoxA1+) cells is significantly increased in the luminal progenitor subset relative to that in control mice
• expression of Ly6a (lymphocyte antigen 6 complex, locus A), an antigen linked to ER and PR expression, is also significantly increased in luminal progenitor cells
• treatment with exogenous beta-estradiol has no significant effect on the % of ERalpha+ ductal cells
• mammary glands from 6-week-old virgin mice show a significant increase in the % of ERalpha+ cells in the mammary stem (MaSC)/basal cell population (CD29hiCD24+) which normally lacks estrogen receptor (Esr1, ERalpha) and progesterone receptor (Pgr, PR) expression
• Foxa1 mRNA and protein levels are dramatically increased in MaSC/basal cells relative to those in control mice
• at estrus, mice display abnormally-shaped nodular ovaries that contain many large follicles in a loosely connected medulla
• 6-week-old virgin mice show a significant increase in maturing follicle numbers, consistent with an increase in Fshr (follicle stimulating hormone receptor) mRNA levels
• 6-week-old virgin mice show a significant increase in the number of secondary and antral ovarian follicles per ovary relative to control mice
• 6-week-old virgin mice show a significant increase in the number of secondary, antral and atretic (degenerating) ovarian follicles
• however, the total number of follicles per ovary is similar to that in control mice
• at estrus, the ovarian medulla appears loosely connected

reproductive system
• at estrus, mice display abnormally-shaped nodular ovaries that contain many large follicles in a loosely connected medulla
• 6-week-old virgin mice show a significant increase in maturing follicle numbers, consistent with an increase in Fshr (follicle stimulating hormone receptor) mRNA levels
• 6-week-old virgin mice show a significant increase in the number of secondary and antral ovarian follicles per ovary relative to control mice
• 6-week-old virgin mice show a significant increase in the number of secondary, antral and atretic (degenerating) ovarian follicles
• however, the total number of follicles per ovary is similar to that in control mice
• at estrus, the ovarian medulla appears loosely connected
• 6-week-old mice staged at either estrus or proestrus show a significant reduction in uterus wet weight relative to control mice
• treatment with exogenous beta-estradiol restores uterus wet weight to control values

homeostasis/metabolism
• 6-week-old mice staged at either estrus or proestrus show a significant reduction in uterus wet weight relative to control mice, indicating reduced levels of circulating estrogen
• ovaries from 6-week-old virgin mice exhibit a significant reduction in the expression of key estradiol biosynthesis enzymes relative to control ovaries

integument
• mammary glands from 4-week-old virgin mice display ductal elongation defects, as determined by the % of fat pad filled relative to control mice
• although ductal elongation is largely restored by 8 weeks of age, complex branching of the ducts is still lacking
• treatment with exogenous beta-estradiol at proestrus and estrus rescues the ductal elongation defects
• mammary glands from 6-week-old virgin mice show a significant increase in the % of estrogen receptor (ER)-alpha expressing (ERalpha+) and progesterone receptor expressing (PR+) luminal cells relative to those in control mice
• a significantly increased % of ERalpha+ ductal cells is found in the luminal progenitor subset (CD29loCD24+CD49b+) but not in the mature luminal subset (CD29loCD24+CD49b-)
• % of Foxa1 (forkhead box A1) expressing (FoxA1+) cells is significantly increased in the luminal progenitor subset relative to that in control mice
• expression of Ly6a (lymphocyte antigen 6 complex, locus A), an antigen linked to ER and PR expression, is also significantly increased in luminal progenitor cells
• treatment with exogenous beta-estradiol has no significant effect on the % of ERalpha+ ductal cells
• mammary glands from 6-week-old virgin mice show a significant increase in the % of ERalpha+ cells in the mammary stem (MaSC)/basal cell population (CD29hiCD24+) which normally lacks estrogen receptor (Esr1, ERalpha) and progesterone receptor (Pgr, PR) expression
• Foxa1 mRNA and protein levels are dramatically increased in MaSC/basal cells relative to those in control mice




Genotype
MGI:5805280
cn21
Allelic
Composition
Id4tm1Vdr/Id4+
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Id4tm1Vdr mutation (0 available); any Id4 mutation (7 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• mammary glands from 4-week-old virgin mice display intermediate ductal elongation defects, as determined by the % of fat pad filled

integument
• mammary glands from 4-week-old virgin mice display intermediate ductal elongation defects, as determined by the % of fat pad filled




Genotype
MGI:5469591
cn22
Allelic
Composition
Pik3catm1Gne/Pik3ca+
Tg(MMTV-cre)1Mam/0
Genetic
Background
involves: C57BL/6N * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3catm1Gne mutation (0 available); any Pik3ca mutation (19 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
N
• male mice do not develop tumors in the mammary gland or seminal vesicles
• thoracic and inguino-abdominal mammary gland tumors with latency dependent on nulliparous or multiparous condition
• however, treatment with PI3K inhibitor inhibits tumor growth

integument
• precocious lobulo-alveolar development and hyper-branched ductal structures (2-fold increase in ductal branch point) at 12 weeks
• feathery, hyper-branched ductal tree by 50 weeks
• thoracic and inguino-abdominal mammary gland tumors with latency dependent on nulliparous or multiparous condition
• however, treatment with PI3K inhibitor inhibits tumor growth

endocrine/exocrine glands
• feathery, hyper-branched ductal tree by 50 weeks
• precocious lobulo-alveolar development and hyper-branched ductal structures (2-fold increase in ductal branch point) at 12 weeks
• thoracic and inguino-abdominal mammary gland tumors with latency dependent on nulliparous or multiparous condition
• however, treatment with PI3K inhibitor inhibits tumor growth

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
breast cancer DOID:1612 OMIM:114480
J:193377




Genotype
MGI:5308737
cn23
Allelic
Composition
Becn1tm1Ebr/Becn1+
Tg(MMTV-cre)1Mam/?
Genetic
Background
involves: FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Becn1tm1Ebr mutation (0 available); any Becn1 mutation (29 available)
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• only 2 of 100 neonates survive through day 1
• high level of death associated with heart and brain defects at embryonic days 11.5 and 12.5

reproductive system
• non uniform distribution of primordial follicles
• reduced number of primordial follicles

cardiovascular system
• high level of death associated with heart defects at embryonic days 11.5 and 12.5

nervous system
• high level of death associated with brain defects at embryonic days 11.5 and 12.5

endocrine/exocrine glands
• non uniform distribution of primordial follicles
• reduced number of primordial follicles




Genotype
MGI:4888636
tg24
Allelic
Composition
Tg(MMTV-cre)1Mam/?
Genetic
Background
involves: FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(MMTV-cre)1Mam mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• 38% of pups nursed by mothers carrying this transgene survive to weaning
• pups nursed by mothers carrying this transgene are runts, with weights at weaning about 40% that of those nursed by control mothers

endocrine/exocrine glands
• mammary gland development checked at the end of lactation was highly variable among mice
• cre expression as determined using Gt(ROSA)26Sortm1Sor as a reporter is detected in new born females

integument
• mammary gland development checked at the end of lactation was highly variable among mice
• cre expression as determined using Gt(ROSA)26Sortm1Sor as a reporter is detected in new born females





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/26/2019
MGI 6.14
The Jackson Laboratory