Phenotypes associated with this allele

• Allele Symbol: Tg(Zp3-cre)3Mrt
• Allele Name: transgene insertion 3, Gail R Martin
• Allele ID: MGI:2176052
• Summary: 21 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Kmt2b^tm1Afst/Kmt2b^tm1.1Afst Tg(Zp3-cre)3Mrt/0	involves: 129 * 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:4867494
cn2	Bcas2^tm1.1Lil/Bcas2^tm1.1Lil Tg(Zp3-cre)3Mrt/0	involves: 129 * C57BL/6 * FVB/N * SJL	MGI:5910025
cn3	Tet3^tm1.1Gxu/Tet3^tm1.2Gxu Tg(Zp3-cre)3Mrt/0	involves: 129 * C57BL/6J * FVB/N * SJL	MGI:5294568
cn4	Foxn1^tm1.1Cbln/Foxn1^+ Tg(Zp3-cre)3Mrt/?	involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 * FVB/N	MGI:5297814
cn5	H19^tm6Tilg/H19^tm6Tilg Tg(Zp3-cre)3Mrt/?	involves: 129P2/OlaHsd * C57BL/6	MGI:3714394
cn6	Furin^tm1Jwmc/Furin^tm1Jwmc Tg(Zp3-cre)3Mrt/0	involves: 129P2/OlaHsd * C57BL/6J * CBA/J	MGI:6856673
cn7	Porcn^tm1.1Jrt/Porcn^+ Tg(CAG-EGFP)D4Nagy/0 Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N	MGI:5523431
cn8	Porcn^tm1.1Jrt/Y Tg(CAG-EGFP)D4Nagy/0 Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N	MGI:5523432
cn9	Scn8a^tm1Mm/Scn8a^tm1Mm Tg(Zp3-cre)3Mrt/?	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3050792
cn10	Zeb1^tm2Yhi/Zeb1^tm2Yhi Zeb2^tm1.1Yhi/Zeb2^tm1.1Yhi Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N * ICR	MGI:3653735
cn11	Tfap2c^tm1Will/Tfap2c^tm2Will Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * FVB/N	MGI:3687951
cn12	Tfap2a^tm2Will/Tfap2a^+ Tfap2c^tm1Will/Tfap2c^tm2Will Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * FVB/N	MGI:3687953
cn13	Tfap2a^tm1Hsv/Tfap2a^tm2Will Tfap2c^tm1Will/Tfap2c^tm2Will Tg(Zp3-cre)3Mrt/0	involves: 129S1/Sv * FVB/N	MGI:3687952
cn14	Mybl2^tm1.1Jof/Mybl2^+ Tg(Zp3-cre)3Mrt/0	involves: 129S2/SvPas * C57BL/6 * FVB/N	MGI:5518769
cn15	Mc4r^tm1Lowl/Mc4r^tm1Lowl Tg(Zp3-cre)3Mrt/0	involves: 129S4/SvJae * C57BL/6J * FVB/N	MGI:3692538
cn16	Rasgrf1^tm4.1Pds/Rasgrf1^+ Tg(Zp3-cre)3Mrt/0	involves: 129S4/SvJaeSor * C57BL/6 * FVB/N	MGI:3698138
cn17	Nlrp3^tm1Hhf/Nlrp3^+ Tg(Zp3-cre)3Mrt/?	involves: 129/Sv * FVB/N	MGI:3850044
cn18	Ndc80^tm1Tski/Ndc80^tm1Tski Tg(Zp3-cre)3Mrt/0	involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * FVB/N	MGI:6452499
cn19	Ythdf2^tm1.1Doca/Ythdf2^tm1.1Doca Tg(Zp3-cre)3Mrt/0	involves: FVB/N	MGI:5911743
tg20	Tg(Zp3-cre)3Mrt/Tg(Zp3-cre)3Mrt	FVB/N-Tg(Zp3-cre)3Mrt/J	MGI:3835424
tg21	Tg(Zp3-cre)3Mrt/0	B6.FVB-Tg(Zp3-cre)3Mrt	MGI:3835441

Genotype
MGI:4867494

cn1

• Allelic Composition: Kmt2b^tm1Afst/Kmt2b^tm1.1Afst; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129 * 129P2/OlaHsd * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal ovarian follicle number ( J:166778 )

♀ • increased number of secondary and preantral follicles at 3 weeks old

♀ • decreased number of preantral follicles at 8 weeks old

decreased primordial ovarian follicle number ( J:166778 )

♀ • decreased number of primordial follicles at 3 and 8 weeks old

increased atretic ovarian follicle number ( J:166778 )

♀ • increased number of atretic follicles at 8 weeks old

decreased superovulation rate ( J:166778 )

♀ • reduced ovulation rates (20%-25% of control rates) in 3-week-old superovulated females

♀ • trapped oocytes in luteinizing structures in hormonally stimulated females

female infertility ( J:166778 )

♀

embryo

embryonic growth arrest ( J:166778 )

• embryos generated from matings of mutant females and wild-type males become developmentally arrested between the 1-cell and 4-cell stages

cellular

abnormal DNA methylation ( J:166778 )

• increased mono-methylated H3K4 in oocytes

• decreased tri-methylated H3K4 in oocytes

homeostasis/metabolism

increased circulating follicle stimulating hormone level ( J:166778 )

• increased serum FSH levels at 8 weeks old

endocrine/exocrine glands

abnormal ovarian follicle number ( J:166778 )

♀ • increased number of secondary and preantral follicles at 3 weeks old

♀ • decreased number of preantral follicles at 8 weeks old

decreased primordial ovarian follicle number ( J:166778 )

♀ • decreased number of primordial follicles at 3 and 8 weeks old

increased atretic ovarian follicle number ( J:166778 )

♀ • increased number of atretic follicles at 8 weeks old

Genotype
MGI:5910025

cn2

• Allelic Composition: Bcas2^tm1.1Lil/Bcas2^tm1.1Lil; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129 * C57BL/6 * FVB/N * SJL

mortality/aging

mortality/aging ( J:239876 )

N

reproductive system

female infertility ( J:239876 )

♀

cellular

maternal effect ( J:239876 )

♀

Genotype
MGI:5294568

cn3

• Allelic Composition: Tet3^tm1.1Gxu/Tet3^tm1.2Gxu; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129 * C57BL/6J * FVB/N * SJL

cellular

abnormal DNA methylation ( J:176196 )

• paternal genome reprogramming is impaired in offspring of mutant females

maternal effect ( J:176196 )

• paternal genome reprogramming is impaired in offspring of mutant females

• inheriting the mutant allele maternally impairs embryonic survival

Genotype
MGI:5297814

cn4

• Allelic Composition: Foxn1^tm1.1Cbln/Foxn1⁺; Tg(Zp3-cre)3Mrt/?
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6 * FVB/N

immune system

immune system phenotype ( J:177992 )

N • normal thymus development and function

Genotype
MGI:3714394

cn5

• Allelic Composition: H19^tm6Tilg/H19^tm6Tilg; Tg(Zp3-cre)3Mrt/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cellular

genetic imprinting ( J:57113 )

• imprinting of Igf2 is not altered

Genotype
MGI:6856673

cn6

• Allelic Composition: Furin^tm1Jwmc/Furin^tm1Jwmc; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * CBA/J

reproductive system

abnormal oocyte morphology ( J:272231 )

♀ • loss of connexin 37 staining at the surface of oocytes indicates impaired gap junctions between oocytes and surrounding granulosa cells at the early secondary follicle stage

♀ • metaphase II (MII) oocytes collected after superovulation exhibit poor quality with degenerated first polar bodies and severely abnormal spindles

abnormal primary polar body morphology ( J:272231 )

♀ • metaphase II (MII) oocytes collected after superovulation exhibit degenerated first polar bodies

increased granulosa cell apoptosis ( J:272231 )

♀ • however, no apparent apoptosis is detected in early secondary follicles

♀ • at P35, TUNEL analysis showed severe apoptosis of granulosa cells in late preantral follicles and antral follicles

decreased granulosa cell proliferation ( J:272231 )

♀ • PCNA staining showed a severe defect in granulosa cell proliferation at 2 months of age

decreased mature ovarian follicle number ( J:272231 )

♀

abnormal secondary ovarian follicle number ( J:272231 )

♀ • at 6 and 8 weeks of age, number of early secondary follicles is significantly increased while the number of large growing (late preantral) follicles is markedly decreased

decreased tertiary ovarian follicle number ( J:272231 )

♀ • number of antral follicles is markedly decreased

increased atretic ovarian follicle number ( J:272231 )

♀ • large antral follicles exhibit degeneration at 8 weeks of age; few or almost no late growing follicles or antral follicles are observed, suggesting atresia of follicles beyond the early secondary stage

impaired ovarian folliculogenesis ( J:272231 )

♀ • at 6 weeks of age, the number of early secondary follicles is significantly increased while the number of large growing (late preantral) follicles and antral follicles is markedly decreased, indicating that primary follicles fail to develop beyond the early secondary follicle stage

small ovary ( J:272231 )

♀ • ovaries are smaller at P42

abnormal meiotic spindle morphology ( J:272231 )

♀ • metaphase II (MII) oocytes collected after superovulation exhibit severely abnormal spindles

decreased superovulation rate ( J:272231 )

♀ • following induction of ovulation with exogenous PMSG + hCG, the number of ovulated oocytes per 6-wk-old female is significantly lower than that in control females

anovulation ( J:272231 )

♀ • females fail to ovulate during a natural ovulation assay

failure of superovulation ( J:272231 )

♀ • 8-wk-old or older females cannot be induced to ovulate anymore

female infertility ( J:272231 )

♀ • when mated with fertile males for 6 months, females fail to produce any progeny indicating complete female infertility

cellular

abnormal oocyte morphology ( J:272231 )

♀ • loss of connexin 37 staining at the surface of oocytes indicates impaired gap junctions between oocytes and surrounding granulosa cells at the early secondary follicle stage

♀ • metaphase II (MII) oocytes collected after superovulation exhibit poor quality with degenerated first polar bodies and severely abnormal spindles

abnormal primary polar body morphology ( J:272231 )

♀ • metaphase II (MII) oocytes collected after superovulation exhibit degenerated first polar bodies

increased granulosa cell apoptosis ( J:272231 )

♀ • at P35, TUNEL analysis showed severe apoptosis of granulosa cells in late preantral follicles and antral follicles

♀ • however, no apparent apoptosis is detected in early secondary follicles

decreased granulosa cell proliferation ( J:272231 )

♀ • PCNA staining showed a severe defect in granulosa cell proliferation at 2 months of age

endocrine/exocrine glands

increased granulosa cell apoptosis ( J:272231 )

♀ • at P35, TUNEL analysis showed severe apoptosis of granulosa cells in late preantral follicles and antral follicles

♀ • however, no apparent apoptosis is detected in early secondary follicles

decreased granulosa cell proliferation ( J:272231 )

♀ • PCNA staining showed a severe defect in granulosa cell proliferation at 2 months of age

decreased mature ovarian follicle number ( J:272231 )

♀

abnormal secondary ovarian follicle number ( J:272231 )

♀ • at 6 and 8 weeks of age, number of early secondary follicles is significantly increased while the number of large growing (late preantral) follicles is markedly decreased

decreased tertiary ovarian follicle number ( J:272231 )

♀ • number of antral follicles is markedly decreased

increased atretic ovarian follicle number ( J:272231 )

♀ • large antral follicles exhibit degeneration at 8 weeks of age; few or almost no late growing follicles or antral follicles are observed, suggesting atresia of follicles beyond the early secondary stage

impaired ovarian folliculogenesis ( J:272231 )

♀ • at 6 weeks of age, the number of early secondary follicles is significantly increased while the number of large growing (late preantral) follicles and antral follicles is markedly decreased, indicating that primary follicles fail to develop beyond the early secondary follicle stage

small ovary ( J:272231 )

♀ • ovaries are smaller at P42

Genotype
MGI:5523431

cn7

• Allelic Composition: Porcn^tm1.1Jrt/Porcn⁺; Tg(CAG-EGFP)D4Nagy/0; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N

embryo

embryo phenotype ( J:198636 )

♀N • heterozygous females implant normally when the mutant allele ant the cre transgene are from the mother and the reporter is from the father

Genotype
MGI:5523432

cn8

• Allelic Composition: Porcn^tm1.1Jrt/Y; Tg(CAG-EGFP)D4Nagy/0; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * C57BL/6NCr * FVB/N

embryo

failure to gastrulate ( J:198636 )

♂ • hemizygous males fail to gastrulate when mutant and cre are derived from the mother and the reporter is derived from the father

Genotype
MGI:3050792

cn9

• Allelic Composition: Scn8a^tm1Mm/Scn8a^tm1Mm; Tg(Zp3-cre)3Mrt/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

mortality/aging

premature death ( J:91696 )

• died between 3 and 4 weeks of age

behavior/neurological

ataxia ( J:91696 )

• by 14 days of age

hindlimb paralysis ( J:91696 )

• follows development of ataxia

Genotype
MGI:3653735

cn10

• Allelic Composition: Zeb1^tm2Yhi/Zeb1^tm2Yhi; Zeb2^tm1.1Yhi/Zeb2^tm1.1Yhi; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N * ICR

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:108819 )

• do not survive after E8.5

embryo

failure of initiation of embryo turning ( J:108819 )

• embryo turning does not occur

embryonic growth arrest ( J:108819 )

• developmental arrest around E8.5

abnormal neural plate morphology ( J:108819 )

• defects in neural plate as shown by decreased Sox2 expression

abnormal neural tube morphology ( J:108819 )

• exhibit a marked thinning of the portion that normally forms the dorsal half of the neural tube

open neural tube ( J:108819 )

• neural tube fails to close; exhibit a wider opening of the neural tube than single homozygous Zfhx1b mice

abnormal somite development ( J:108819 )

• somite cleavage stops at somite 7 while embryo elongation continues

• aberrant positioning of somite cleavage with normal production of presomitic mesoderm

decreased somite size ( J:108819 )

• short somites

nervous system

abnormal neural plate morphology ( J:108819 )

• defects in neural plate as shown by decreased Sox2 expression

abnormal neural tube morphology ( J:108819 )

• exhibit a marked thinning of the portion that normally forms the dorsal half of the neural tube

open neural tube ( J:108819 )

• neural tube fails to close; exhibit a wider opening of the neural tube than single homozygous Zfhx1b mice

Genotype
MGI:3687951

cn11

• Allelic Composition: Tfap2c^tm1Will/Tfap2c^tm2Will; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * FVB/N

embryo

embryo phenotype ( J:113442 )

N • blastocyts derived from zygotes lacking both maternal and zygotic Tcfap2c contributions make it through preimplantation development

Genotype
MGI:3687953

cn12

• Allelic Composition: Tfap2a^tm2Will/Tfap2a⁺; Tfap2c^tm1Will/Tfap2c^tm2Will; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * FVB/N

embryo

decreased embryo size ( J:113442 )

• embryos are smaller than wild-type, with morphology similar to published reports of Tcfap2c-null embryos

abnormal embryonic tissue morphology ( J:113442 )

• embryos are more disorganized than wild-type, with morphology similar to published report of Tcfap2c-null embryos

abnormal extraembryonic tissue morphology ( J:113442 )

• embryos are more disorganized than wild-type, with morphology similar to published report of Tcfap2c-null embryos

growth/size/body

decreased embryo size ( J:113442 )

• embryos are smaller than wild-type, with morphology similar to published reports of Tcfap2c-null embryos

Genotype
MGI:3687952

cn13

• Allelic Composition: Tfap2a^tm1Hsv/Tfap2a^tm2Will; Tfap2c^tm1Will/Tfap2c^tm2Will; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S1/Sv * FVB/N

mortality/aging

embryonic lethality between implantation and somite formation, incomplete penetrance ( J:113442 )

• a significant reduction in number of double-null embryos (5.5% vs 25% expected) compared to 3 other possible genotypic combinations results in earlier lethality than loss of either allele alone (~E7.5)

embryo

embryo phenotype ( J:113442 )

N • double-null embryos are found at ~expected frequencies (~25%) at E3.5; embryos are detected in both the morula (~75%) and blastocyst (~25%) stages, indicating that deficiency of both Tcfap2a and Tcfap2c does not affect the zygote prior to E3.5;

N • other genotypic combinations are all found at similar frequencies (~25%) at E7.5, indicating that loss of any combination of 3 of 4 wild-type Tcfap2a and Tcfap2c alleles allows embryo survival to ~E7.5

Genotype
MGI:5518769

cn14

• Allelic Composition: Mybl2^tm1.1Jof/Mybl2⁺; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * FVB/N

hematopoietic system

enlarged spleen ( J:196804 )

• 9 of 13 mice have enlarged spleens at 22 months of age

abnormal myelopoiesis ( J:196804 )

• 12 of 13 mice at 22 months of age show multiple myeloid disorders

• 46% (6 of 13) of mice develop myelodysplasia (MDS)

• 38% (5 of 13) of mice develop myeloproliferative neoplasms (MPN)

• 7% (1 of 13) of mice develop myeloid leukemia

• transplantation of mutant bone marrow cells into lethally irradiated recipients accelerates development of MDS

myeloid hyperplasia ( J:196804 )

• peripheral blood and spleen of mice that develop MDS show an increase in myeloid populations

• 3 of 5 mice that develop MPN, with or without anemia, with or without thrombocytopenia, show an increase in the myeloid population in peripheral blood, bone marrow, and spleen

anemia ( J:196804 )

• signs of anemia and changes in white blood cell counts are detectable between 12 and 18 months of age and exacerbated by 22 months

increased bone marrow cell number ( J:196804 )

• mutants that develop MPN show myeloproliferation in the bone marrow with high cellularity and practically no fat cells

increased megakaryocyte cell number ( J:196804 )

abnormal erythrocyte morphology ( J:196804 )

• peripheral blood of mice that develop MDS shows dyserythropoiesis that includes anisocytosis and poikilocytosis and the presence of Howell-Jolly bodies

decreased erythrocyte cell number ( J:196804 )

• decrease in red blood cells in mice that develop MDS or MPN

anisocytosis ( J:196804 )

• in mice that develop MDS

increased number of Howell-Jolly bodies ( J:196804 )

• in mice that develop MDS

poikilocytosis ( J:196804 )

• in mice that develop MDS

thrombocytopenia ( J:196804 )

• some mice show thrombocytopenia and others thrombocytosis

thrombocytosis ( J:196804 )

• some mice show thrombocytopenia and others thrombocytosis

decreased leukocyte cell number ( J:196804 )

• decrease in white blood cells in mice that develop MDS

decreased neutrophil cell number ( J:196804 )

• peripheral blood of mice that develop MDS shows neutropenia

increased leukocyte cell number ( J:196804 )

• increase in white blood cells in mice that develop MPN

increased granulocyte number ( J:196804 )

• the mouse that develops myeloid leukemia shows a dramatic increase in the percentage of granulocytes

increased monocyte cell number ( J:196804 )

• the mouse that develops myeloid leukemia shows a dramatic increase in the percentage of monocytes

immune system

enlarged spleen ( J:196804 )

• 9 of 13 mice have enlarged spleens at 22 months of age

abnormal myelopoiesis ( J:196804 )

• 12 of 13 mice at 22 months of age show multiple myeloid disorders

• 46% (6 of 13) of mice develop myelodysplasia (MDS)

• 38% (5 of 13) of mice develop myeloproliferative neoplasms (MPN)

• 7% (1 of 13) of mice develop myeloid leukemia

• transplantation of mutant bone marrow cells into lethally irradiated recipients accelerates development of MDS

myeloid hyperplasia ( J:196804 )

• peripheral blood and spleen of mice that develop MDS show an increase in myeloid populations

• 3 of 5 mice that develop MPN, with or without anemia, with or without thrombocytopenia, show an increase in the myeloid population in peripheral blood, bone marrow, and spleen

decreased leukocyte cell number ( J:196804 )

• decrease in white blood cells in mice that develop MDS

decreased neutrophil cell number ( J:196804 )

• peripheral blood of mice that develop MDS shows neutropenia

increased leukocyte cell number ( J:196804 )

• increase in white blood cells in mice that develop MPN

increased granulocyte number ( J:196804 )

• the mouse that develops myeloid leukemia shows a dramatic increase in the percentage of granulocytes

increased monocyte cell number ( J:196804 )

• the mouse that develops myeloid leukemia shows a dramatic increase in the percentage of monocytes

neoplasm

increased leukemia incidence ( J:196804 )

• 7% (1 of 13) of mice develop myeloid leukemia with marked leukocytosis, anemia, and in increase in the number of platelets

• the one mouse that develops a myeloid neoplasm shows the mild increase in platelet number and anemia at 12 to 18 months of age, whereas the massive increase of white blood cells starts after 18 months of age

• the mouse that develops a myeloid neoplasm shows immature myeloid cells in the blood, exhibits white bones, splenomegaly, and disruption of spleen architecture with loss of lymphatic follicles and an increase in the number of megakaryocytes and infiltration of myeloid cells in the liver

growth/size/body

enlarged spleen ( J:196804 )

• 9 of 13 mice have enlarged spleens at 22 months of age

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
acute myeloid leukemia		DOID:9119	OMIM:601626	J:196804
myelodysplastic syndrome		DOID:0050908	OMIM:614286	J:196804

Genotype
MGI:3692538

cn15

• Allelic Composition: Mc4r^tm1Lowl/Mc4r^tm1Lowl; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6J * FVB/N

normal phenotype

no abnormal phenotype detected ( J:115192 )

• mice homozygous for reactivated Mc4r in the germline show normal body weights and rescue of the increased snout-anus length observed in Mc4r^tm1Lowl homozygotes

Genotype
MGI:3698138

cn16

• Allelic Composition: Rasgrf1^tm4.1Pds/Rasgrf1⁺; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: 129S4/SvJaeSor * C57BL/6 * FVB/N

cellular

abnormal cell physiology ( J:117666 )

• Rasgrf1 expression from paternal allele is silenced in neonatal brain

abnormal imprinting ( J:117666 )

• DNA from progeny has lost almost all differentially methylated domain (DMD) methylation from the paternal allele

• methylation on floxed allele cannot be maintained upon cre-mediated recombination in zygote

Genotype
MGI:3850044

cn17

• Allelic Composition: Nlrp3^tm1Hhf/Nlrp3⁺; Tg(Zp3-cre)3Mrt/?
• Genetic Background: involves: 129/Sv * FVB/N

mortality/aging

postnatal lethality, complete penetrance ( J:150054 )

• mice die between 2 and 14 days after birth probably due to multisystem organ failure secondary to inflammation and necrosis

growth/size/body

slow postnatal weight gain ( J:150054 )

• mutant pups gained weight slowly, and then lost weight before dying

immune system

increased leukocyte cell number ( J:150054 )

• white blood cell count is mildly elevated

increased neutrophil cell number ( J:150054 )

• pronounced neutrophilia is evident in these mice

increased inflammatory response ( J:150054 )

• mice have pronounced leukocytic infiltrates in skin, liver, spleen, joint, sinus, conjunctiva, bone marrow, and tongue that are mainly neutrophilic

digestive/alimentary system

abnormal intestine morphology ( J:150054 )

• substantial necrosis occurs in the gut that is not associated with inflammation

renal/urinary system

abnormal kidney morphology ( J:150054 )

• substantial necrosis occurs in the kidney that is not associated with inflammation

hematopoietic system

thrombocytosis ( J:150054 )

• thrombocytosis is evident in these mice

increased leukocyte cell number ( J:150054 )

• white blood cell count is mildly elevated

increased neutrophil cell number ( J:150054 )

• pronounced neutrophilia is evident in these mice

integument

hairless ( J:150054 )

• hair growth does not occur in these mice

reddish skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

scaly skin ( J:150054 )

• 1-2 day old mice have skin abscesses that develop into erythema and scaly skin by day 4

Genotype
MGI:6452499

cn18

• Allelic Composition: Ndc80^tm1Tski/Ndc80^tm1Tski; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: C57BL/6 * C57BL/6NCrlj * CBA/JNCrlj * FVB/N

cellular

aneuploidy ( J:292307 )

• aberrant anaphase I in oocytes results in egg aneuploidies

reproductive system

abnormal meiotic spindle assembly checkpoint ( J:292307 )

• oocytes undergo aberrant anaphase I accompanied by degradation of securing, indicating spindle checkpoint defects; aberrant anaphase results in egg aneuploidies

abnormal meiotic spindle morphology ( J:292307 )

• the microtubule mass undergoes dynamic and aberrant shape changes in oocytes and fails to establish a stable bipolar-shaped spindle during meiosis I, resulting in severe kinetochore-microtubule attachment defects, with some chromosomes falling off the microtubule mass

• although oocytes frequently establish a stable bipolar-shaped spindle in meiosis II, bipolar-shaped spindles exhibit massive chromosome misalignment in metaphase II

Genotype
MGI:5911743

cn19

• Allelic Composition: Ythdf2^tm1.1Doca/Ythdf2^tm1.1Doca; Tg(Zp3-cre)3Mrt/0
• Genetic Background: involves: FVB/N

cellular

maternal effect ( J:243747 )

• fertilized zygotes fail to develop beyond the two-cell stage

reproductive system

female infertility ( J:243747 )

♀ • despite the presence of corpora lutea, normal induced ovulation and normal fertilization

Genotype
MGI:3835424

tg20

• Allelic Composition: Tg(Zp3-cre)3Mrt/Tg(Zp3-cre)3Mrt
• Genetic Background: FVB/N-Tg(Zp3-cre)3Mrt/J

reproductive system

reduced female fertility ( J:67903 )

♀ • females are subfertile, producing an average of 3.7 pups per litter

Genotype
MGI:3835441

tg21

• Allelic Composition: Tg(Zp3-cre)3Mrt/0
• Genetic Background: B6.FVB-Tg(Zp3-cre)3Mrt

reproductive system

reduced female fertility ( J:67903 )

♀ • females are subfertile, producing an average of 2.5 pups per litter