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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Cd86tm2Shr
targeted mutation 2, Arlene H Sharpe
MGI:2159105
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
129.SJL-Cd86tm2Shr MGI:3620768
cx2
Cd86tm2Shr/Cd86tm2Shr
Icostm1Flv/Icostm1Flv
involves: 129S1/Sv * 129S4/SvJaeSor MGI:3851527
cx3
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
involves: 129S4/SvJae MGI:3618330
cx4
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
involves: 129S4/SvJae * BALB/c MGI:3662675
cx5
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Ctla4tm1Shr/Ctla4tm1Shr
involves: 129S4/SvJae * BALB/c MGI:3714352
cx6
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
involves: 129S4/SvJae * C57BL/6J * SJL MGI:3620769
cx7
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Tg(Ighm-Ctla4Ig/Cd80)1Jbs/0
involves: 129S4/SvJae * FVB/N * NOD MGI:3714277
cx8
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
involves: 129S4/SvJae * NOD MGI:3620124


Genotype
MGI:3620768
cx1
Allelic
Composition
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Genetic
Background
129.SJL-Cd86tm2Shr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd80tm1Shr mutation (11 available); any Cd80 mutation (40 available)
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• proliferative responses of lymph node cells to myelin proteolipid protein peptide 139-151 or 178-191 are impaired in null mice compared to wild-type

immune system
• no IL-2 production could be detected in draining lymph node cells
• mice develop EAE, but severity is lower and time of onset is delayed compared to wild-type SJL mice
• Background Sensitivity: null mice on a 129 background are susceptible to EAE, whereas nulls on a B6 background are resistant

nervous system
• in mice exhibiting EAE, there are lesions in the meniges and parenchyma of the spinal cord and brain; number of lesions is lower than in wild-type mice with EAE
• lesions associated with white matter vacuolation occur with higher incidence in null mice compared to wild-type mice with EAE




Genotype
MGI:3851527
cx2
Allelic
Composition
Cd86tm2Shr/Cd86tm2Shr
Icostm1Flv/Icostm1Flv
Genetic
Background
involves: 129S1/Sv * 129S4/SvJaeSor
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
Icostm1Flv mutation (3 available); any Icos mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• T cells from mice immunized with keyhole limpet hemocyanin (KLH) in CFA (conjugated Freund's adjuvant) produce no IL-4, IL-17a or interferon gamma in response to KLH restimulation

homeostasis/metabolism
• T cells from mice immunized with keyhole limpet hemocyanin (KLH) in CFA (conjugated Freund's adjuvant) produce no IL-4, IL-17a or interferon gamma in response to KLH restimulation




Genotype
MGI:3618330
cx3
Allelic
Composition
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Genetic
Background
involves: 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd80tm1Shr mutation (11 available); any Cd80 mutation (40 available)
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• during primary response after antigen stimulation, T cells produce less than cells that are additionally Ctla4-null

immune system
• mice have 0.3% CD4+CD25hiCD62L+ regulatory T cells compared to 4% in NOD controls (J:113109)
• in the thymus (J:205441)
• after immunization with complete Freund's adjuvant, spleens examined 7-10 days later were significantly smaller than wild-type
• mutants have a 3 to 5-fold elevation in IgG3
• unimmunized mice show a 2 to 5-fold reduction in total serum IgG and IgG2a and a 5 to 10-fold reduction in IgG1
• mutants have a 3 to 5-fold elevation in IgM
• cells secrete more interferon gamma and less Il-4 than Ctla4-null cells
• during primary response after antigen stimulation, T cells produce less than cells that are additionally Ctla4-null
• lymphoid follicles are small, resembling primary follicles without recognizable germinal centers
• 100% of mice develop diabetes by 17 weeks

hematopoietic system
• mice have 0.3% CD4+CD25hiCD62L+ regulatory T cells compared to 4% in NOD controls (J:113109)
• in the thymus (J:205441)
• after immunization with complete Freund's adjuvant, spleens examined 7-10 days later were significantly smaller than wild-type
• mutants have a 3 to 5-fold elevation in IgG3
• unimmunized mice show a 2 to 5-fold reduction in total serum IgG and IgG2a and a 5 to 10-fold reduction in IgG1
• mutants have a 3 to 5-fold elevation in IgM
• cells secrete more interferon gamma and less Il-4 than Ctla4-null cells
• during primary response after antigen stimulation, T cells produce less than cells that are additionally Ctla4-null




Genotype
MGI:3662675
cx4
Allelic
Composition
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Genetic
Background
involves: 129S4/SvJae * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd80tm1Shr mutation (11 available); any Cd80 mutation (40 available)
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• mice have increased CD4/CD8 ratios in the spleen (4.1 vs 2.2 in controls) and in lymph nodes (6.6 vs 2.8 in controls), reciprocal to the decreased ratios seen in transgenic Cd86 or Cd80 mice
• ther are fewer double positive cells (74.3%) compared to controls (83.8%) and higher CD4+ cell numbers (17.2% vs 10.5%)

immune system
• mice have increased CD4/CD8 ratios in the spleen (4.1 vs 2.2 in controls) and in lymph nodes (6.6 vs 2.8 in controls), reciprocal to the decreased ratios seen in transgenic Cd86 or Cd80 mice
• ther are fewer double positive cells (74.3%) compared to controls (83.8%) and higher CD4+ cell numbers (17.2% vs 10.5%)




Genotype
MGI:3714352
cx5
Allelic
Composition
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Ctla4tm1Shr/Ctla4tm1Shr
Genetic
Background
involves: 129S4/SvJae * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd80tm1Shr mutation (11 available); any Cd80 mutation (40 available)
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
Ctla4tm1Shr mutation (4 available); any Ctla4 mutation (25 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• during secondary stimulation with anti-CD3 and antigen-presenting cells, CD4+ T cells proliferate more than Ctla4-sufficient, CD80/86 null cells

immune system
• T cells differentiate into Th2 cells upon anti-CD3 stimulus
• during secondary stimulation with anti-CD3 and antigen-presenting cells, CD4+ T cells proliferate more than Ctla4-sufficient, CD80/86 null cells
• cells produce Il-4 even during primary stimulation with anti-CD3 and APCs, whereas Ctla4-sufficient mice do not, and on secondary stimulation, cells produce more Il-4 and less interferon gamma than Ctla4-sufficient cells
• upon anti-CD28 stimulation in vivo, cells produce Il-4 but wild-type cells do not; mice show less interferon gamma production after anti-CD28 treatment than wild-type mice

hematopoietic system
• T cells differentiate into Th2 cells upon anti-CD3 stimulus
• during secondary stimulation with anti-CD3 and antigen-presenting cells, CD4+ T cells proliferate more than Ctla4-sufficient, CD80/86 null cells




Genotype
MGI:3620769
cx6
Allelic
Composition
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Genetic
Background
involves: 129S4/SvJae * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd80tm1Shr mutation (11 available); any Cd80 mutation (40 available)
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• lymph node cells from null animals proliferate poorly compared to wild-type, but do produce IFNG in response to PLP or MOG peptides

immune system
• null mice on the mixed background are resistant to EAE when immunized with either PLP 139-151 or myelin oligodendrocyte glycoprotein 35-55 compared with wild-type (B6 x SJL)F1 mice

nervous system
• numbers of foci in the meninges and parenchyma of the spinal cords of null mice are lower compared to control mice




Genotype
MGI:3714277
cx7
Allelic
Composition
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Tg(Ighm-Ctla4Ig/Cd80)1Jbs/0
Genetic
Background
involves: 129S4/SvJae * FVB/N * NOD
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd80tm1Shr mutation (11 available); any Cd80 mutation (40 available)
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
Tg(Ighm-Ctla4Ig/Cd80)1Jbs mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mice show less insulitis compared to non-transgenic double nulls
• mice have 0.3% CD4+CD25hiCD62L+ regulatory T cells compared to 4% in NOD controls
• mice show 40% suppression of diabetes development compared to non-transgenic double null animals

hematopoietic system
• mice have 0.3% CD4+CD25hiCD62L+ regulatory T cells compared to 4% in NOD controls

endocrine/exocrine glands
• mice show less insulitis compared to non-transgenic double nulls




Genotype
MGI:3620124
cx8
Allelic
Composition
Cd80tm1Shr/Cd80tm1Shr
Cd86tm2Shr/Cd86tm2Shr
Genetic
Background
involves: 129S4/SvJae * NOD
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd80tm1Shr mutation (11 available); any Cd80 mutation (40 available)
Cd86tm2Shr mutation (2 available); any Cd86 mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• at 9 weeks, all islets isolated from male and female homozygous mice showed high infiltration, whereas in wild-type NOD mice, only 10% and 3% of islets obtained from female and male mice displayed severe insulitis; no insulitis is detected at 4 weeks and at 6 weeks most islets aren't infiltrated
• there is a profound decrease in numbers of CD4+CD25+ T cells in homozygotes compared to wild-type with levels lower than in CD28-null mice on a NOD background
• in T cells and supernatants collected after stimulation with antil-CD3, production of all cytokines is reduced compared to wild-type levels
• almost 100% of homozygous mice become diabetic (blood glucose >300 mg/dl on 2 consecutive measurements) by 18 weeks of age, compared to 70% of female and 20% of male wild-type NOD controls or 40% of female and 10% of male wild-type mice

endocrine/exocrine glands
• at 9 weeks, all islets isolated from male and female homozygous mice showed high infiltration, whereas in wild-type NOD mice, only 10% and 3% of islets obtained from female and male mice displayed severe insulitis; no insulitis is detected at 4 weeks and at 6 weeks most islets aren't infiltrated

hematopoietic system
• there is a profound decrease in numbers of CD4+CD25+ T cells in homozygotes compared to wild-type with levels lower than in CD28-null mice on a NOD background

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
J:61905





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last database update
03/03/2020
MGI 6.15
The Jackson Laboratory