Analysis Tools
immune system
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• homozygotes exhibit a severely impaired lymphocyte migration to the Peyer's pathces and the B cell follicles of the spleen, resulting in morphologically abnormal primary lymphoid follicles
• activated B cells fail to migrate from the T cell-rich zone into B cell follicles of the spleen
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• homozygotes display a 2- to 4-fold increase in spleen or peripheral blood cells co-expressing B220 and high amounts fo IgM
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• at 8 wks of age, homozygotes display defective formation of primary splenic follicles, including: the T cell zone is located centrally but not polarized in the follicle; the T cell zone is completely surrounded by a small rim of B cell-expressing IgD but not IgM (i.e. IgM+IgD+ subset is absent); a prominent marginal zone surrounds the follicle completely
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• despite high numbers of germinal center founder cells, no functional germinal centers develop in mutant spleen after immunization with the T cell-dependent antigen DNP-KHL
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• absence of primary follicular dendritic cells
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• ~30% (26 of 76) of homozygotes exhibit 1-4 small and rudimentary structures resembling Peyer's patches
• ~16% (12 of 76) of homozygotes exhibit normally sized Peyer's patches with multiple B and T cell-rich zones instead of the large follicles present in wild-type mice
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• 55% (42 of 76) of homozygotes show complete absence of Peyer's patches
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• homozygotes lack inguinal lymph nodes
(J:37126)
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• absence of inguinal, iliac/periaortic, axillary, branchial, deep cervical, sacral/caudal, and parathymic lymph nodes
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hematopoietic system
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• homozygotes exhibit a severely impaired lymphocyte migration to the Peyer's pathces and the B cell follicles of the spleen, resulting in morphologically abnormal primary lymphoid follicles
• activated B cells fail to migrate from the T cell-rich zone into B cell follicles of the spleen
|
|
• homozygotes display a 2- to 4-fold increase in spleen or peripheral blood cells co-expressing B220 and high amounts fo IgM
|
|
• at 8 wks of age, homozygotes display defective formation of primary splenic follicles, including: the T cell zone is located centrally but not polarized in the follicle; the T cell zone is completely surrounded by a small rim of B cell-expressing IgD but not IgM (i.e. IgM+IgD+ subset is absent); a prominent marginal zone surrounds the follicle completely
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• despite high numbers of germinal center founder cells, no functional germinal centers develop in mutant spleen after immunization with the T cell-dependent antigen DNP-KHL
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cellular
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• homozygotes exhibit a severely impaired lymphocyte migration to the Peyer's pathces and the B cell follicles of the spleen, resulting in morphologically abnormal primary lymphoid follicles
• activated B cells fail to migrate from the T cell-rich zone into B cell follicles of the spleen
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