Mouse Genome Informatics
hm1
    Lama2tm1Stk/Lama2tm1Stk
involves: 129/Sv * 129P2/OlaHsd * BALB/c * ICR
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• mice die of undetermined causes by 5 weeks of age

muscle
• by P21 and P28, proliferation of endomysial connective tissue is observed
• the number of apoptotic nuclei in muscle fibers is increased compared to in wild-type mice
• by P21 and P28, muscle fibers of different calibers are apparent
• by P24 mice exhibit dystrophic symptoms including a waddling gait and twitching
• by P24 mice exhibit twitching
• by P9 mice exhibit scattered degeneration of muscle fibers and at P11 numerous degenerating fibers with extensive infiltrate are observed
• degeneration is accompanied by regeneration at P13

behavior/neurological
• by P24 mice exhibit a waddling gait

growth/size
• beginning at P14 and continuing for the rest of their lives
• apparent after postnatal day 14

cellular
• the basal lamina is nearly completely absent around muscle fibers and Schwann cells of peripheral nerves


Mouse Genome Informatics
hm2
    Lama2tm1Stk/Lama2tm1Stk
involves: 129P2/OlaHsd
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
reproductive system
• at day 23, no sperm flagellum are detected in the testis (J:100711)
• the basement membrane underlying Sertoli cells is thinner and disrupted compared to in wild-type mice (J:100711)
• small or no lumens form in the seminiferous tubules unlike in wild-type mice (J:100711)
• fewer spermatid are detected by protamine-2 staining compared to in wild-type mice (J:100711)

endocrine/exocrine glands
• at day 23, no sperm flagellum are detected in the testis (J:100711)
• the basement membrane underlying Sertoli cells is thinner and disrupted compared to in wild-type mice (J:100711)
• small or no lumens form in the seminiferous tubules unlike in wild-type mice (J:100711)

cellular
• the basement membrane underlying Sertoli cells is thinner and disrupted compared to in wild-type mice


Mouse Genome Informatics
hm3
    Lama2tm1Stk/Lama2tm1Stk
involves: 129P2/OlaHsd * BALB/c
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• the cortex is thin with few thymocytes
• the cortico-medullary junction is obscured
• unlike in wild-type mice, many apoptotic cells are present in the subcapsular and outer cortical areas of the thymus
• total numbers of thymic and splenic T cells are reduced
• the number of double positive thymocytes is decreased compared to in wild-type mice likely due to increased apoptosis

hematopoietic system
• the cortex is thin with few thymocytes
• the cortico-medullary junction is obscured
• unlike in wild-type mice, many apoptotic cells are present in the subcapsular and outer cortical areas of the thymus
• total numbers of thymic and splenic T cells are reduced
• the number of double positive thymocytes is decreased compared to in wild-type mice likely due to increased apoptosis

endocrine/exocrine glands
• the cortex is thin with few thymocytes
• the cortico-medullary junction is obscured
• unlike in wild-type mice, many apoptotic cells are present in the subcapsular and outer cortical areas of the thymus


Mouse Genome Informatics
cx4
    Lama2tm1Stk/Lama2tm1Stk
Lama4tm1Ktry/Lama4tm1Ktry

involves: 129P2/OlaHsd * 129X1/SvJ
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• while appearing normal at birth and suckling effectively, mice die by P13

nervous system
• at P11, mice exhibit a near complete absence of radial sorting of spinal nerve roots and distal nerves
• proliferating Schwann cells in E17 mice is decreased


Mouse Genome Informatics
cx5
    Lama2tm1Stk/Lama2tm1Stk
Tg(CAG-Lama1)12Mdur/0

involves: 129P2/OlaHsd * C57BL/6 * CBA
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
reproductive system
N
• mice are capable of reproduction and exhibit a rescue of the reproductive defects observed in Lama2tm1Stk homozygotes (J:100711)