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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lama2tm1Stk
targeted mutation 1, Shin'ichi Takeda
MGI:2153004
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Lama2tm1Stk/Lama2tm1Stk involves: 129P2/OlaHsd MGI:3789206
hm2
Lama2tm1Stk/Lama2tm1Stk involves: 129P2/OlaHsd * BALB/c MGI:3789293
hm3
Lama2tm1Stk/Lama2tm1Stk involves: 129/Sv * 129P2/OlaHsd * BALB/c * ICR MGI:3789197
cx4
Lama2tm1Stk/Lama2tm1Stk
Lama4tm1Ktry/Lama4tm1Ktry
involves: 129P2/OlaHsd * 129X1/SvJ MGI:3789289
cx5
Lama2tm1Stk/Lama2tm1Stk
Tg(CAG-Lama1)12Mdur/0
involves: 129P2/OlaHsd * C57BL/6 * CBA MGI:3789207


Genotype
MGI:3789206
hm1
Allelic
Composition
Lama2tm1Stk/Lama2tm1Stk
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lama2tm1Stk mutation (1 available); any Lama2 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• fewer spermatid are detected by protamine-2 staining compared to in wild-type mice
• at day 23, no sperm flagellum are detected in the testis
• the basement membrane underlying Sertoli cells is thinner and disrupted compared to in wild-type mice
• small or no lumens form in the seminiferous tubules unlike in wild-type mice

endocrine/exocrine glands
• at day 23, no sperm flagellum are detected in the testis
• the basement membrane underlying Sertoli cells is thinner and disrupted compared to in wild-type mice
• small or no lumens form in the seminiferous tubules unlike in wild-type mice

cellular
• fewer spermatid are detected by protamine-2 staining compared to in wild-type mice
• the basement membrane underlying Sertoli cells is thinner and disrupted compared to in wild-type mice




Genotype
MGI:3789293
hm2
Allelic
Composition
Lama2tm1Stk/Lama2tm1Stk
Genetic
Background
involves: 129P2/OlaHsd * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lama2tm1Stk mutation (1 available); any Lama2 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• the cortex is thin with few thymocytes
• the cortico-medullary junction is obscured
• unlike in wild-type mice, many apoptotic cells are present in the subcapsular and outer cortical areas of the thymus
• total numbers of thymic and splenic T cells are reduced
• the number of double positive thymocytes is decreased compared to in wild-type mice likely due to increased apoptosis

hematopoietic system
• the cortex is thin with few thymocytes
• the cortico-medullary junction is obscured
• unlike in wild-type mice, many apoptotic cells are present in the subcapsular and outer cortical areas of the thymus
• total numbers of thymic and splenic T cells are reduced
• the number of double positive thymocytes is decreased compared to in wild-type mice likely due to increased apoptosis

endocrine/exocrine glands
• the cortex is thin with few thymocytes
• the cortico-medullary junction is obscured
• unlike in wild-type mice, many apoptotic cells are present in the subcapsular and outer cortical areas of the thymus




Genotype
MGI:3789197
hm3
Allelic
Composition
Lama2tm1Stk/Lama2tm1Stk
Genetic
Background
involves: 129/Sv * 129P2/OlaHsd * BALB/c * ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lama2tm1Stk mutation (1 available); any Lama2 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mice die of undetermined causes by 5 weeks of age

muscle
• by P21 and P28, proliferation of endomysial connective tissue is observed
• the number of apoptotic nuclei in muscle fibers is increased compared to in wild-type mice
• by P21 and P28, muscle fibers of different calibers are apparent
• by P24 mice exhibit dystrophic symptoms including a waddling gait and twitching
• by P24 mice exhibit twitching
• by P9 mice exhibit scattered degeneration of muscle fibers and at P11 numerous degenerating fibers with extensive infiltrate are observed
• degeneration is accompanied by regeneration at P13

behavior/neurological
• by P24 mice exhibit a waddling gait

growth/size/body
• beginning at P14 and continuing for the rest of their lives
• apparent after postnatal day 14

cellular
• the basal lamina is nearly completely absent around muscle fibers and Schwann cells of peripheral nerves




Genotype
MGI:3789289
cx4
Allelic
Composition
Lama2tm1Stk/Lama2tm1Stk
Lama4tm1Ktry/Lama4tm1Ktry
Genetic
Background
involves: 129P2/OlaHsd * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lama2tm1Stk mutation (1 available); any Lama2 mutation (71 available)
Lama4tm1Ktry mutation (1 available); any Lama4 mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• while appearing normal at birth and suckling effectively, mice die by P13

nervous system
• at P11, mice exhibit a near complete absence of radial sorting of spinal nerve roots and distal nerves
• proliferating Schwann cells in E17 mice is decreased




Genotype
MGI:3789207
cx5
Allelic
Composition
Lama2tm1Stk/Lama2tm1Stk
Tg(CAG-Lama1)12Mdur/0
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lama2tm1Stk mutation (1 available); any Lama2 mutation (71 available)
Tg(CAG-Lama1)12Mdur mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
N
• mice are capable of reproduction and exhibit a rescue of the reproductive defects observed in Lama2tm1Stk homozygotes





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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory