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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Pdx1tm1Ted
targeted mutation 1, Thomas Edlund
MGI:2152755
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Pdx1tm1Ted/Pdx1tm1Ted involves: 129P2/OlaHsd MGI:2173182
ht2
Pdx1tm1Ted/Pdx1+ involves: 129P2/OlaHsd MGI:3531547
cn3
Insrtm1Khn/Insrtm1Khn
Pdx1tm1Ted/Pdx1+
Tg(Alb-cre)21Mgn/0
involves: 129S4/SvJae * C57BL/6 * DBA/2 MGI:3583116


Genotype
MGI:2173182
hm1
Allelic
Composition
Pdx1tm1Ted/Pdx1tm1Ted
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pdx1tm1Ted mutation (0 available); any Pdx1 mutation (17 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all homozygotes die within a few days after birth (J:20779)
• all homozygotes die within a few days after birth (J:20779)

digestive/alimentary system
• mutant intestines are of normal length and show no overt abnormalities; however, the loops of the small intestine are positioned slightly differently in the abdomen relative to wild-type (J:20779)
• mutant intestines are of normal length and show no overt abnormalities; however, the loops of the small intestine are positioned slightly differently in the abdomen relative to wild-type (J:20779)

endocrine/exocrine glands
• at E10, the mutant dorsal pancreatic epithelium forms a bud but then becomes growth arrested and fails to lobulate (J:33133)
• at E11 and E12, the dorsal bud is significantly smaller than wild-type; the remaining, growth-arrested bud remains unbranched (J:33133)
• at E10-E12, the mutant ventral pancreatic bud appears to be absent (J:33133)
• although the mutant pancreatic epithelium is growth-arrested, the dorsal pancreatic mesenchyme grows and develops normally, with a morphologically normal, bud-like shape found at its normal position (J:33133)
• notably, the mutant mesenchyme is functionally active and able to promote morphogenesis and differentiation of pancreatic epithelium when recombined in vitro; in contrast, mutant pancreatic epithelium cannot be rescued by adding wild-type mesenchyme (J:33133)
• at E13.5, the mutant dorsal pancreatic mesenchyme is present as a "hollow pocket" that can be dissected away from the stomach and duodenum; in wild-type E13-E14 embryos, this mesenchymal pocket is filled by pancreatic epithelium (J:33133)
• at E10, early glucagon cells are present but are unable to proliferate and/or differentiate; a few early insulin cells are present at E10, but are no longer detectable at E13, in the absence of increased apoptosis (J:33133)
• at E10, the mutant dorsal pancreatic epithelium forms a bud but then becomes growth arrested and fails to lobulate (J:33133)
• at E11 and E12, the dorsal bud is significantly smaller than wild-type; the remaining, growth-arrested bud remains unbranched (J:33133)
• at E10-E12, the mutant ventral pancreatic bud appears to be absent (J:33133)
• although the mutant pancreatic epithelium is growth-arrested, the dorsal pancreatic mesenchyme grows and develops normally, with a morphologically normal, bud-like shape found at its normal position (J:33133)
• notably, the mutant mesenchyme is functionally active and able to promote morphogenesis and differentiation of pancreatic epithelium when recombined in vitro; in contrast, mutant pancreatic epithelium cannot be rescued by adding wild-type mesenchyme (J:33133)
• at E13.5, the mutant dorsal pancreatic mesenchyme is present as a "hollow pocket" that can be dissected away from the stomach and duodenum; in wild-type E13-E14 embryos, this mesenchymal pocket is filled by pancreatic epithelium (J:33133)
• at E10, early glucagon cells are present but are unable to proliferate and/or differentiate; a few early insulin cells are present at E10, but are no longer detectable at E13, in the absence of increased apoptosis (J:33133)
• homozygotes completely lack a pancreas; no pancreatic tissue is present in E15.5 embryos or neonates (J:20779)
• notably, the duodenum (from which the pancreas develops) has a normal C-shaped form; no ectopic expression of insulin and pancreatic amylase is detected in the developing duodenum (J:20779)
• the pancreatic duct is missing; however, the common bile duct is present, indicating that apart from the lack of pancreas, the duodenal tract is normally developed (J:20779)
• homozygotes completely lack a pancreas; no pancreatic tissue is present in E15.5 embryos or neonates (J:20779)
• notably, the duodenum (from which the pancreas develops) has a normal C-shaped form; no ectopic expression of insulin and pancreatic amylase is detected in the developing duodenum (J:20779)
• the pancreatic duct is missing; however, the common bile duct is present, indicating that apart from the lack of pancreas, the duodenal tract is normally developed (J:20779)

growth/size/body
• homozygotes appear morphologically normal but are slightly smaller than wild-type and heterozygous littermates: on average, ~80% for newborn pups and ~60% for 2-day-old pups (J:20779)
• homozygotes appear morphologically normal but are slightly smaller than wild-type and heterozygous littermates: on average, ~80% for newborn pups and ~60% for 2-day-old pups (J:20779)

homeostasis/metabolism
• pups that are able to feed and live for more than 2 days exhibit elevated glucose levels, suggesting that the cause of death is partly due to insulin deficiency (J:20779)
• pups that are able to feed and live for more than 2 days exhibit elevated glucose levels, suggesting that the cause of death is partly due to insulin deficiency (J:20779)

immune system
N
• mutant spleens are of normal weight and show no apparent abnormalities relative to wild-type (J:20779)
• mutant spleens are of normal weight and show no apparent abnormalities relative to wild-type (J:20779)

liver/biliary system
N
• mutant livers are of normal weight and show no apparent abnormalities relative to wild-type (J:20779)
• mutant livers are of normal weight and show no apparent abnormalities relative to wild-type (J:20779)




Genotype
MGI:3531547
ht2
Allelic
Composition
Pdx1tm1Ted/Pdx1+
Genetic
Background
involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pdx1tm1Ted mutation (0 available); any Pdx1 mutation (17 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• islet isolation procedures generate very few islets (always <100) from heterozygotes compared with wild-type mice (almost always >200 islets) (J:82969)
• isolated heterozygous islets appear fragmented and slightly smaller than wild-type (J:82969)
• in vivo, heterozygotes show a striking disruption of islet architecture and expansion of islet microvasculature at 3 months; however, even at 12 months, some heterozygous islets show no signs of damage or apoptosis (J:82969)
• in heterozygotes, islet number fails to increase with age and is ~50% less than wild-type by 12 months (J:82969)
• islet isolation procedures generate very few islets (always <100) from heterozygotes compared with wild-type mice (almost always >200 islets) (J:82969)
• isolated heterozygous islets appear fragmented and slightly smaller than wild-type (J:82969)
• in vivo, heterozygotes show a striking disruption of islet architecture and expansion of islet microvasculature at 3 months; however, even at 12 months, some heterozygous islets show no signs of damage or apoptosis (J:82969)
• in heterozygotes, islet number fails to increase with age and is ~50% less than wild-type by 12 months (J:82969)
• in heterozygotes, beta cell mass fails to increase with age and is ~50% less than wild-type by 12 months (J:82969)
• reduced beta cell mass is mirrored by a lower pancreatic insulin content in older mice and can be explained by a reduced number of functional islets, rather than a reduction in insulin stored in single beta cells (J:82969)
• in heterozygotes, beta cell mass fails to increase with age and is ~50% less than wild-type by 12 months (J:82969)
• reduced beta cell mass is mirrored by a lower pancreatic insulin content in older mice and can be explained by a reduced number of functional islets, rather than a reduction in insulin stored in single beta cells (J:82969)
• heterozygotes display normal depolarization-evoked single-cell exocytosis and voltage-gated Ca2+ currents in beta cells (J:82969)
• however, isolated heterozygous islets and dispersed beta cells are significantly more susceptible to apoptosis at basal glucose concentrations than wild-type islets; no significant differences are noted at high glucose (25 mM) or Ca2+ stress (J:82969)
• heterozygotes display normal depolarization-evoked single-cell exocytosis and voltage-gated Ca2+ currents in beta cells (J:82969)
• however, isolated heterozygous islets and dispersed beta cells are significantly more susceptible to apoptosis at basal glucose concentrations than wild-type islets; no significant differences are noted at high glucose (25 mM) or Ca2+ stress (J:82969)
• perfused pancreata from heterozygous males display attenuated first-phase insulin secretion in response to a stepwise increase in glucose concentration from 5 mM to 20 mM glucose (J:82969)
• also, mutant pancreata show a reduced insulin release in response to 20 mM KCl; in the continued presence of 26 mM glucose, the large response to 20 mM arginine is also reduced (J:82969)
• notably, insulin secretion in perifusion or static incubation experiments in response to glucose and other secretagogues is similar in islets isolated from heterozygous mutants compared with wild-type littermates (J:82969)
• glucose sensing and islet Ca2+ responses are also normal in large, intact heterozygous mutant islets (J:82969)
• perfused pancreata from heterozygous males display attenuated first-phase insulin secretion in response to a stepwise increase in glucose concentration from 5 mM to 20 mM glucose (J:82969)
• also, mutant pancreata show a reduced insulin release in response to 20 mM KCl; in the continued presence of 26 mM glucose, the large response to 20 mM arginine is also reduced (J:82969)
• notably, insulin secretion in perifusion or static incubation experiments in response to glucose and other secretagogues is similar in islets isolated from heterozygous mutants compared with wild-type littermates (J:82969)
• glucose sensing and islet Ca2+ responses are also normal in large, intact heterozygous mutant islets (J:82969)
• at 12 months, older heterozygotes exhibit infiltration of lymphocytes (some TUNEL-positive) within or around islets (J:82969)
• immunofluorescent localization of Ki67 nuclear antigen indicates proliferation of lymphocytes within these islets (J:82969)
• at 12 months, older heterozygotes exhibit infiltration of lymphocytes (some TUNEL-positive) within or around islets (J:82969)
• immunofluorescent localization of Ki67 nuclear antigen indicates proliferation of lymphocytes within these islets (J:82969)

homeostasis/metabolism
• perfused pancreata from heterozygous males display attenuated first-phase insulin secretion in response to a stepwise increase in glucose concentration from 5 mM to 20 mM glucose (J:82969)
• also, mutant pancreata show a reduced insulin release in response to 20 mM KCl; in the continued presence of 26 mM glucose, the large response to 20 mM arginine is also reduced (J:82969)
• notably, insulin secretion in perifusion or static incubation experiments in response to glucose and other secretagogues is similar in islets isolated from heterozygous mutants compared with wild-type littermates (J:82969)
• glucose sensing and islet Ca2+ responses are also normal in large, intact heterozygous mutant islets (J:82969)
• perfused pancreata from heterozygous males display attenuated first-phase insulin secretion in response to a stepwise increase in glucose concentration from 5 mM to 20 mM glucose (J:82969)
• also, mutant pancreata show a reduced insulin release in response to 20 mM KCl; in the continued presence of 26 mM glucose, the large response to 20 mM arginine is also reduced (J:82969)
• notably, insulin secretion in perifusion or static incubation experiments in response to glucose and other secretagogues is similar in islets isolated from heterozygous mutants compared with wild-type littermates (J:82969)
• glucose sensing and islet Ca2+ responses are also normal in large, intact heterozygous mutant islets (J:82969)
• heterozygous mutant males exhibit significantly elevated blood glucose concentrations relative to wild-type males (J:82969)
• notably, heterozygous females display an attenuated increase in blood glucose levels relative to wild-type (J:82969)
• heterozygous mutant males exhibit significantly elevated blood glucose concentrations relative to wild-type males (J:82969)
• notably, heterozygous females display an attenuated increase in blood glucose levels relative to wild-type (J:82969)
• heterozygotes exhibit a decline in glucose tolerance with increasing age (J:82969)
• heterozygotes exhibit a decline in glucose tolerance with increasing age (J:82969)

immune system
• at 12 months, older heterozygotes exhibit infiltration of lymphocytes (some TUNEL-positive) within or around islets (J:82969)
• immunofluorescent localization of Ki67 nuclear antigen indicates proliferation of lymphocytes within these islets (J:82969)
• at 12 months, older heterozygotes exhibit infiltration of lymphocytes (some TUNEL-positive) within or around islets (J:82969)
• immunofluorescent localization of Ki67 nuclear antigen indicates proliferation of lymphocytes within these islets (J:82969)




Genotype
MGI:3583116
cn3
Allelic
Composition
Insrtm1Khn/Insrtm1Khn
Pdx1tm1Ted/Pdx1+
Tg(Alb-cre)21Mgn/0
Genetic
Background
involves: 129S4/SvJae * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Insrtm1Khn mutation (1 available); any Insr mutation (30 available)
Pdx1tm1Ted mutation (0 available); any Pdx1 mutation (17 available)
Tg(Alb-cre)21Mgn mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• these mutants fail to exhibit an adaptive islet hyperplastic response to insulin resistance; instead, pancreatic beta cell mass is reduced, and islets appear small with scattered non-beta cells (J:92861)
• these mutants fail to exhibit an adaptive islet hyperplastic response to insulin resistance; instead, pancreatic beta cell mass is reduced, and islets appear small with scattered non-beta cells (J:92861)





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last database update
02/02/2016
MGI 6.02
The Jackson Laboratory