Mouse Genome Informatics
hm1
    Maftm1Glm/Maftm1Glm
involves: 129/Sv * BALB/c
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype

Histological analysis of the lens from Maftm1Glm/Maf+ and Maftm1Glm/Maftm1Glm mice

mortality/aging
• intrauterine death of some homozygotes occurs at E17.5-18.5; only 8% of live-born pups are homozygous
• about one-third of homozygotes do not survive past weaning
• intrauterine death of some homozygotes occurs at E17.5-18.5; only 8% of live-born pups are homozygous

growth/size/body
• from P15 onward, homozygotes appear runted compared to wild-type littermates

vision/eye
N
• despite lens defects, cornea is well separated from lens and formation of neural retina is grossly normal (J:54079)
• in 5-week old adults, lens shows substantial degeneration
• cells of the posterior region of the lens fail to elongate toward the epithelial anterior wall and differentiate into lens fiber cells, resulting in a small and hollow lens cavity
• defect is apparent by E12
• homozygotes display microphthalmia, identifiable from P15 onward

immune system
• cultured Maf-deficient cells have severe impairment in Il-4 production compared to wild-type cells: Maf-deficient mice injected with anti-CD3 show a defect in Il-4 induction
• Maf-deficient splenocytes treated with exogenous Il-4 produce 70% lower levels of Il-4; purified naive T helper cells produce almost no Il-4 after treatment with exogenous Il-4
• expression of other cytokines is not directly affected in mutants