Phenotypes associated with this allele

• Allele Symbol: Penk-rs^tm1Pig
• Allele Name: targeted mutation 1, Pain Investigation Group
• Allele ID: MGI:2136456
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Penk-rs^tm1Pig/Penk-rs^tm1Pig	B6.129-Penk-rs^tm1Pig	MGI:3623104
hm2	Penk-rs^tm1Pig/Penk-rs^tm1Pig	B6.129-Penk-rs^tm1Pig/J	MGI:3623105
hm3	Penk-rs^tm1Pig/Penk-rs^tm1Pig	involves: 129S1/Sv * 129X1/SvJ * CD-1	MGI:3623103

Genotype
MGI:3623104

hm1

• Allelic Composition: Penk-rs^tm1Pig/Penk-rs^tm1Pig
• Genetic Background: B6.129-Penk-rs^tm1Pig

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

decreased behavioral withdrawal response ( J:66732 )

• decrease in the severity of THC withdrawal signs (ptosis, piloerection, mastication, body tremor, paw tremor, ataxia, and hunched posture) compared to wild-type mice

decreased chemically-elicited antinociception ( J:66732 )

• THC induced antinociceptive response is significantly less intense in homozygotes than in wild-type mice

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:66732 )

• delta9-tetrahydrocannadinol (THC) induced weight loss is reduced

• however, no difference in the effect on locomotion is seen and no difference in the induction of tolerance to THC is seen

immune system

decreased T-helper 2 cell number ( J:62435 )

• fewer IL5 producing CD4+ T cells are found in helminth parasite infected homozygotes; however, no difference in the number of CD4+ T cells in the lung is seen

abnormal T-helper 2 physiology ( J:62435 )

• production of IL4 and IL5 by CD4+ T cells is reduced in helminth parasite infected homozygotes

• however, levels of IgE and IgG1 in helminth parasite infected or OVA sensitized homozygotes is similar to wild-type mice

lung inflammation ( J:62435 )

• OVA-sensitization and helminth parasite-induced eosinophil infiltration is reduced 2-fold compared to wild-type mice

hematopoietic system

decreased T-helper 2 cell number ( J:62435 )

• fewer IL5 producing CD4+ T cells are found in helminth parasite infected homozygotes; however, no difference in the number of CD4+ T cells in the lung is seen

abnormal T-helper 2 physiology ( J:62435 )

• production of IL4 and IL5 by CD4+ T cells is reduced in helminth parasite infected homozygotes

• however, levels of IgE and IgG1 in helminth parasite infected or OVA sensitized homozygotes is similar to wild-type mice

respiratory system

lung inflammation ( J:62435 )

• OVA-sensitization and helminth parasite-induced eosinophil infiltration is reduced 2-fold compared to wild-type mice

Genotype
MGI:3623105

hm2

• Allelic Composition: Penk-rs^tm1Pig/Penk-rs^tm1Pig
• Genetic Background: B6.129-Penk-rs^tm1Pig/J

behavior/neurological

behavior/neurological phenotype ( J:107954 )

N • no difference in ethanol intake or preference is seen compared to wild-type mice

impaired behavioral response to nicotine ( J:98226 )

• the severity of nicotine withdrawal is reduced in homozygous mice

• the rewarding effect of nicotine in a place-conditioning assay is reduced in homozygotes compared to wild-type mice

• however, nicotine-induced decreases in locomotor activity are similar to wild-type

decreased behavioral withdrawal response ( J:98226 )

• the severity of nicotine withdrawal is reduced in homozygous mice

abnormal associative learning ( J:98226 )

• the rewarding effect of nicotine in a place-conditioning assay is reduced in homozygotes compared to wild-type mice; however, nicotine-induced decreases in locomotor activity are similar to wild-type

decreased chemically-elicited antinociception ( J:98226 )

• nicotine induced antinociceptive responses are significantly reduced in homozygotes compared to in wild-type mice

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:98226 )

• 0.5 mg/kg nicotine significantly increases extracellular levels of dopamine in the nucleus accumbens of wild-type mice but not in homozygous mice

Genotype
MGI:3623103

hm3

• Allelic Composition: Penk-rs^tm1Pig/Penk-rs^tm1Pig
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CD-1

behavior/neurological

abnormal emotion/affect behavior ( J:35826 )

decreased exploration in new environment ( J:35826 )

• homozygotes show reduced levels of horizontal but not vertical activity during an open field test

increased aggression towards humans ( J:35826 )

• seen in some adults

increased aggression towards mice ( J:35826 )

• in a resident-intruder test, resident homozygotes are immediately aggressive towards the intruder mouse

increased anxiety-related response ( J:35826 )

• pups and adolescent mice display frantic running or jumping, hide under the bedding, and show prolonged freezing response to moderate noise or movement

• in an elevated O-maze, homozygotes spend less time in the open sections

increased thigmotaxis ( J:35826 )

• homozygotes spend more time on the edges during an open field test

abnormal touch/ nociception ( J:35826 )

abnormal chemical nociception ( J:35826 )

• after formalin injection into a hind paw, homozygotes display a different suite of behaviors including, paw shaking, increased agitation, and intense sniffing, but do not lick or lift the injected paw

decreased thermal nociceptive threshold ( J:35826 )

• when placed on a hot plate homozygotes shake their hind paws almost immediately unlike wild-type mice

• however, analgesia induced by stress (forced swim in either 34 degree C or 4 degree C water) is similar that in wild-type mice and pain response in a tail-flick test is similar to wild-type

reproductive system

reproductive system phenotype ( J:35826 )

N • homozygotes are fertile and care for their offspring