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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Krastm3Tyj
targeted mutation 3, Tyler Jacks
MGI:2136169
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
ht1
Krastm3Tyj/Kras+ involves: 129S4/SvJae MGI:3770517
ht2
Krastm3Tyj/Kras+ involves: 129S4/SvJae * C57BL/6 MGI:3770516
cx3
Ccne1tm1Jro/?
Krastm3Tyj/?
either: (involves: 129S4/SvJaeSor) or (involves: 129S4/SvJaeSor * C57BL/6) MGI:3586557
cx4
Eif4eGt(RRO036)Byg/Eif4e+
Krastm3Tyj/Kras+
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * FVB/N MGI:5708384
cx5
Krastm3Tyj/Kras+
Pik3catm1Jdo/Pik3catm1Jdo
involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6J MGI:3716661
cx6
Krastm3Tyj/Kras+
Tg(CAG-Mir21,-EGFP)#Eno/0
involves: 129S4/SvJae * C3H * C57BL/6 MGI:5317786
cx7
Dmtf1tm1Cjs/Dmtf1+
Krastm3Tyj/Kras+
involves: 129S4/SvJae * C57BL/6 MGI:3770617
cx8
Dmtf1tm1Cjs/Dmtf1tm1Cjs
Krastm3Tyj/Kras+
involves: 129S4/SvJae * C57BL/6 MGI:3770618
cx9
Krastm3Tyj/Kras+
Mir21atm1.1Eno/Mir21atm1.1Eno
involves: 129S/SvEv * 129S4/SvJae * C57BL/6 MGI:5317787


Genotype
MGI:3770517
ht1
Allelic
Composition
Krastm3Tyj/Kras+
Genetic
Background
involves: 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• Background Sensitivity: ~30% of animals develop thymic lymphoma; incidence is higher than on pure 129/Sv background

respiratory system
• 100% of mice examined at 3-4 months of age have developed lung tumors

mortality/aging
• Background Sensitivity: mean age at death is ~250 days on pure 129/Sv background compared to 200 days on mixed background

neoplasm
• Background Sensitivity: ~30% of animals develop thymic lymphoma; incidence is higher than on pure 129/Sv background
• Background Sensitivity: about 40% of mice develop skin papillomas, with slightly higher incidence than on mixed 129 and C57BL/6 background
• 100% of mice examined at 3-4 months of age have developed lung tumors

integument
• Background Sensitivity: about 40% of mice develop skin papillomas, with slightly higher incidence than on mixed 129 and C57BL/6 background

Mouse Models of Human Disease
OMIM ID Ref(s)
Lung Cancer 211980 J:68981




Genotype
MGI:3770516
ht2
Allelic
Composition
Krastm3Tyj/Kras+
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• 100% of animals show multifocal tumors at time of death/sacrifice
• tumors are detectable at 1 week of age as small pleural nodules; multiplicity and size of tumors increases with age, and ultimately result in respiratory distress and death
• tumors range from mild hyperplasia/dysplasia to overt carcinoma; small lesions consist of hyperplastic alveolar epithelium
• in older mice, infrequent metastases to thoracic lymph nodes, the kidney and other visceral organs
• as lesions enlarge, they form small alveolar adenomas; some show areas of glandular differentiation
• 5-10% of tumors have features of well-differentiated human papillary adenocarcinoma

mortality/aging
• Background Sensitivity: mean age at death is ~200 days

neoplasm
• all animals at time of death/sacrifice have extensive tumor burden
• mice display a more rapid tumor phenotype than Krastm2Tyj heterozygotes
• Background Sensitivity: animals are prone to thymic lymphomas (seen in ~40% of animals)
• Background Sensitivity: animals are prone to skin papillomas (seen in ~20% of animals); tumors typically arising on ears and snout are predominantly pedunculated and show limited tendency to progress to squamous cell carcinoma
• 100% of animals show multifocal tumors at time of death/sacrifice
• tumors are detectable at 1 week of age as small pleural nodules; multiplicity and size of tumors increases with age, and ultimately result in respiratory distress and death
• tumors range from mild hyperplasia/dysplasia to overt carcinoma; small lesions consist of hyperplastic alveolar epithelium
• in older mice, infrequent metastases to thoracic lymph nodes, the kidney and other visceral organs
• as lesions enlarge, they form small alveolar adenomas; some show areas of glandular differentiation
• 5-10% of tumors have features of well-differentiated human papillary adenocarcinoma

digestive/alimentary system
• mutants have multiple aberrant crypt foci (ACF) of the colon

endocrine/exocrine glands
• mutants have multiple aberrant crypt foci (ACF) of the colon
• Background Sensitivity: animals are prone to thymic lymphomas (seen in ~40% of animals)

integument
• Background Sensitivity: animals are prone to skin papillomas (seen in ~20% of animals); tumors typically arising on ears and snout are predominantly pedunculated and show limited tendency to progress to squamous cell carcinoma

Mouse Models of Human Disease
OMIM ID Ref(s)
Lung Cancer 211980 J:68981




Genotype
MGI:3586557
cx3
Allelic
Composition
Ccne1tm1Jro/?
Krastm3Tyj/?
Genetic
Background
either: (involves: 129S4/SvJaeSor) or (involves: 129S4/SvJaeSor * C57BL/6)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ccne1tm1Jro mutation (0 available); any Ccne1 mutation (6 available)
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• mean survival is reduced to 160 days compared to 260 days for mice homozygous for Kras alone

cellular
• 2 of 6 tumors from double mutants display significant gains or losses of whole chromosomes while no gains or losses of whole chromosomes are seen in Kras homozygotes




Genotype
MGI:5708384
cx4
Allelic
Composition
Eif4eGt(RRO036)Byg/Eif4e+
Krastm3Tyj/Kras+
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Eif4eGt(RRO036)Byg mutation (0 available); any Eif4e mutation (30 available)
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
• smaller tumors of mice treated with piperlongumine than in Krastm3Tyj heterozygotes
• fewer tumors by 12 weeks with a more than 2-fold reduction in tumor burden compared with Krastm3Tyj heterozygotes

cellular
• in tumors of mice treated with piperlongumine
• increased compared with Krastm3Tyj heterozygotes




Genotype
MGI:3716661
cx5
Allelic
Composition
Krastm3Tyj/Kras+
Pik3catm1Jdo/Pik3catm1Jdo
Genetic
Background
involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
Pik3catm1Jdo mutation (1 available); any Pik3ca mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
neoplasm
• zero to two visible tumors are found compared to forty or so tumors in Krastm3Tyj mice
• apoptotic rates in small lesions are increased compared to in Krastm3Tyj mice
• one mouse did not show any evidence of lung tumor formation up to 6 months of age




Genotype
MGI:5317786
cx6
Allelic
Composition
Krastm3Tyj/Kras+
Tg(CAG-Mir21,-EGFP)#Eno/0
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
Tg(CAG-Mir21,-EGFP)#Eno mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• at 18 weeks compared with Krastm3Tyj heterozygotes
• mice develop increased incidence of all tumor grades without increasing the rate of conversion to adenocarcinoma compared with Krastm3Tyj heterozygotes

mortality/aging
• mice exhibit premature death compared with wild-type mice
• however, mice exhibit the same mortality as Krastm3Tyj heterozygotes

neoplasm
• increased proliferation of tumor cells compared with tumor cells from Krastm3Tyj heterozygotes
• at 18 weeks compared with Krastm3Tyj heterozygotes
• mice develop increased incidence of all tumor grades without increasing the rate of conversion to adenocarcinoma compared with Krastm3Tyj heterozygotes
• of thymic tumors compared with Krastm3Tyj heterozygotes




Genotype
MGI:3770617
cx7
Allelic
Composition
Dmtf1tm1Cjs/Dmtf1+
Krastm3Tyj/Kras+
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dmtf1tm1Cjs mutation (1 available); any Dmtf1 mutation (55 available)
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• ~35% of mice develop thymic lymphomas

respiratory system
• ~50% of largest tumors are well, moderately, or poorly differentiated adenocarcinomas, with signs of intrabronchial or intravascular invasion
• all mice develop lung adenomas or lung adenocarcinomas
• greater than 50% of mice develop lung carcinomas by 40 weeks of age, and tumors are significantly larger than those found in KrasLA+/- single mutants
• number of tumor nodules increase as animals become sick, with trend to increased nodule size

mortality/aging
• mean survival time of double mutants is 21 weeks compared to 35 weeks for Kras+/-, Dmtf1-sufficient mice

neoplasm
• ~35% of mice develop thymic lymphomas
• 10-20% of animals develop tail papillomas
• <5% of animals show distant metastases (liver/intr-abdominal or leg) of lung tumors, while no metastases are observed in KrasLA+/-, Dmtf1-sufficient mice
• ~50% of largest tumors are well, moderately, or poorly differentiated adenocarcinomas, with signs of intrabronchial or intravascular invasion
• all mice develop lung adenomas or lung adenocarcinomas
• greater than 50% of mice develop lung carcinomas by 40 weeks of age, and tumors are significantly larger than those found in KrasLA+/- single mutants
• number of tumor nodules increase as animals become sick, with trend to increased nodule size
• tumorigenesis is accelerated relative to heterozygous Kras, wild-type Dmtf1 mice

integument
• 10-20% of animals develop tail papillomas




Genotype
MGI:3770618
cx8
Allelic
Composition
Dmtf1tm1Cjs/Dmtf1tm1Cjs
Krastm3Tyj/Kras+
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dmtf1tm1Cjs mutation (1 available); any Dmtf1 mutation (55 available)
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• all mice develop lung adenomas or lung adenocarcinomas
• greater than 50% of mice develop lung carcinomas by 40 weeks of age, and tumors are significantly larger than those found in KrasLA+/- single mutants
• number of tumor nodules increase as animals become sick, with trend to increased nodule size

endocrine/exocrine glands
• ~20% of mice develop thymic lymphomas

mortality/aging
• mean survival time of double mutants is 21 weeks compared to 35 weeks for Kras+/-, Dmtf1-sufficient mice

neoplasm
• ~20% of mice develop thymic lymphomas
• 10-20% of animals develop tail papillomas
• all mice develop lung adenomas or lung adenocarcinomas
• greater than 50% of mice develop lung carcinomas by 40 weeks of age, and tumors are significantly larger than those found in KrasLA+/- single mutants
• number of tumor nodules increase as animals become sick, with trend to increased nodule size
• tumorigenesis is accelerated relative to heterozygous Kras, wild-type Dmtf1 mice

integument
• 10-20% of animals develop tail papillomas




Genotype
MGI:5317787
cx9
Allelic
Composition
Krastm3Tyj/Kras+
Mir21atm1.1Eno/Mir21atm1.1Eno
Genetic
Background
involves: 129S/SvEv * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm3Tyj mutation (2 available); any Kras mutation (32 available)
Mir21atm1.1Eno mutation (0 available); any Mir21a mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• fewer than in Krastm3Tyj heterozygotes

neoplasm
N
• unlike Krastm3Tyj heterozygotes, mice do not develop skin papillomas or lung adenomcarcinomas
• fewer than in Krastm3Tyj heterozygotes
• mice exhibit decreased total tumor area relative to lung area compared with Krastm3Tyj heterozygotes
• however, tumor cell proliferation is the same as in Krastm3Tyj heterozygotes
• mice develop fewer lung adenoma than in Krastm3Tyj heterozygotes
• mice develop fewer thymic lymphoma, lung hyperplasia and lung adenomas than in Krastm3Tyj heterozygotes
• fewer lung adenoma than in Krastm3Tyj heterozygotes

respiratory system
• mice develop fewer lung adenoma than in Krastm3Tyj heterozygotes
• mice develop fewer lung hyperplasia than in Krastm3Tyj heterozygotes





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last database update
07/19/2016
MGI 6.04
The Jackson Laboratory