Phenotypes associated with this allele

• Allele Symbol: Dag1^tm1Kcam
• Allele Name: targeted mutation 1, Kevin P Campbell
• Allele ID: MGI:1934902
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Dag1^tm1Kcam/Dag1^tm1Kcam	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:3656076
cn2	Dag1^tm1Kcam/Dag1^tm2.1Kcam Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:5470527
cn3	Dag1^tm1Kcam/Dag1^tm2Kcam Tg(GFAP-cre)25Mes/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:2684282

Genotype
MGI:3656076

cn1

• Allelic Composition: Dag1^tm1Kcam/Dag1^tm1Kcam
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:40601 )

• most homozygotes are substantially resorbed by E10.5

embryo

failure to gastrulate ( J:40601 )

• homozygotes fail to gastrulate

embryonic growth arrest ( J:40601 )

• homozygotes fail to progress beyond the early egg cylinder stage of development

• however, early differentiation of extraembryonic lineages and implantation appear unaffected

decreased embryo size ( J:40601 )

• by E7.5, homozygous mutant embryos are much smaller than wild-type embryos

abnormal embryonic tissue morphology ( J:40601 )

• starting at E6.5, homozygotes typically have a significantly smaller embryonic region

• only a small mass of embryonic tissue is detected at E10.5

absent mesoderm ( J:40601 )

• at E7.5, homozygotes lack any identifiable mesodermal tissue

abnormal extraembryonic tissue morphology ( J:40601 )

absent extraembryonic ectoderm ( J:40601 )

• at E6.5, homozygotes lack any significant development of extraembryonic ectoderm

• in contrast, visceral endoderm and a well-formed pro-amniotic cavity are clearly evident

abnormal Reichert's membrane morphology ( J:40601 )

• at E6.5, homozygotes lack a continuous Reichert's membrane; maternal red blood cells are thus found in the yolk sac cavity as early as E5.5

• only occasional "patches" of laminin and collagen IV, two major structural elements of Reichert's membrane, are detected

• in contrast, the basement membrane between the visceral endoderm and ectoderm appears normal

• Reichert's membrane defects are not due to a lack of parietal endoderm

growth/size/body

decreased embryo size ( J:40601 )

• by E7.5, homozygous mutant embryos are much smaller than wild-type embryos

Genotype
MGI:5470527

cn2

• Allelic Composition: Dag1^tm1Kcam/Dag1^tm2.1Kcam; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

nervous system

abnormal axon fasciculation ( J:194150 )

• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice

abnormal axon guidance ( J:194150 )

• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice

radial glial endfoot detachment ( J:194150 )

• endfoot detachment

abnormal spinal cord ventral commissure morphology ( J:194150 )

• at E11.5, commissural axons exhibit robust postcrossing trajectory defects with failure to project to the lateral portion of the funiculus and altered lateral and ventral funiculi ratio compared with wild-type mice

• at E13, a large number of commissural axons project abnormally within the floor plate unlike in wild-type mice

• at E13.5, mice exhibit extensive disruptions in more lateral aspect of the ventrolateral funiculus compared with wild-type mice

abnormal spinal cord dorsal column morphology ( J:194150 )

• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice

cellular

abnormal axon fasciculation ( J:194150 )

• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice

abnormal axon guidance ( J:194150 )

• mice exhibit abnormal formation of the descending hindbrain axonal tract and severe defasciculation of the spinal cord dorsal funiculus compared with wild-type mice

radial glial endfoot detachment ( J:194150 )

• endfoot detachment

abnormal basement membrane morphology ( J:194150 )

• at E11.5, mice exhibit progressive fragmentation of the basement membrane surrounding the spinal cord which is accompanied by detachment of radial neuroepithelial endfeet from the basal surface unlike wild-type mice

Genotype
MGI:2684282

cn3

• Allelic Composition: Dag1^tm1Kcam/Dag1^tm2Kcam; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

nervous system

abnormal neuronal migration ( J:86901 )

• cerebral, cerebellar and brain stem neuronal migration abnormalities

increased brain size ( J:86901 )

cellular

abnormal neuronal migration ( J:86901 )

• cerebral, cerebellar and brain stem neuronal migration abnormalities

growth/size/body

megacephaly ( J:86901 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
lissencephaly		DOID:0050453	OMIM:PS607432	J:86901