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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
P2rx3tm1Ckn
targeted mutation 1, Debra A Cockayne
MGI:1934019
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
P2rx3tm1Ckn/P2rx3tm1Ckn involves: 129P2/OlaHsd * C57BL/6 MGI:2687354
cx2
P2rx2tm1Ckn/P2rx2tm1Ckn
P2rx3tm1Ckn/P2rx3tm1Ckn
involves: 129P2/OlaHsd * C57BL/6 MGI:2687353


Genotype
MGI:2687354
hm1
Allelic
Composition
P2rx3tm1Ckn/P2rx3tm1Ckn
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
P2rx3tm1Ckn mutation (1 available); any P2rx3 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
N
• adult homozygotes (4-6 months of age) display normal resting ventilation during normoxia as well as normal ventilatory responses to varying levels of hypoxia (15%, 10%, and 7.5% O2) relative to wild-type controls

cardiovascular system
N
• adult homozygotes display normal carotid body function, as shown by a similar hypoxia-induced increase in sinus nerve activity in carotid body-sinus nerve preparations from homozygous mutant and wild-type mice
• no significant differences in the single-unit response to hypoxia are observed between mutant and wild-type carotid body-sinus nerve preparations (7.73 +/- 0.69 spikes/sec versus 8.22 +/- 0.74 spikes/sec, respectively)




Genotype
MGI:2687353
cx2
Allelic
Composition
P2rx2tm1Ckn/P2rx2tm1Ckn
P2rx3tm1Ckn/P2rx3tm1Ckn
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
P2rx2tm1Ckn mutation (1 available); any P2rx2 mutation (21 available)
P2rx3tm1Ckn mutation (1 available); any P2rx3 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• only ~10% of double homozygotes survive through weaning, without displaying any gross pathological abnormalities in adulthood
• >90% of double homozygotes die between birth and weaning showing pulmonary infection and bronchial pneumonia

respiratory system
N
• surviving double homozygotes display normal ventilatory responses to varying levels of hypercapnia relative to wild-type controls: no significant differences in respiratory rate (fR) and tidal volume (VT) are noted at hypercapnic conditions when CO2 levels increase to 3% and then to 6%
• double homozygotes that die between birth and weaning exhibit bronchial pneumonia, as shown by postmortem examination
• in response to hypoxia (10% O2), adult double homozygotes (4-6 months of age) display a significantly reduced ventilatory response showing an increase of only 129 +/- 154 ml/min/kg in minute ventilation (VE) relative to 979 +/- 161 ml/min/kg in wild-type controls
• in response to further hypoxia (7.5% O2), double homozygotes display a severe ventilatory depression with the VE reduced below prehypoxic levels by 555 +/- 137 ml/min/kg, unlike wild-type controls where no additional increase in ventilation is observed but the VE remains above normoxic levels
• however, adult double homozygotes display normal resting ventilation during normoxia and show normal ventilatory responses to a mild (15% O2) hypoxia relative to wild-type controls

cardiovascular system
• unlike in in vitro carotid body-sinus nerve preparations from wild-type mice where sinus nerve discharge increases from baseline to a peak of 130.82 +/- 10.79 spikes/sec during hypoxia, the afferent discharge in preparations from double mutant mice only reaches a peak of 32.80 +/- 6.97 spikes/sec; this response is even smaller than that observed in preparations from single P2rx2tm1Ckn mice (58.13 +/- 9.40 spikes/sec)
• the average peak firing rate of single units induced by hypoxia is significantly lower in carotid body-sinus nerve preparations from double mutant mice (1.19 +/- 0.10 spikes/sec) relative to that observed in preparations from wild-type (7.76 +/- 0.99 spikes/sec) and single P2rx2tm1Ckn mutant mice (1.64 +/- 0.11 spikes/sec)

immune system
• double homozygotes that die between birth and weaning exhibit bronchial pneumonia, as shown by postmortem examination

homeostasis/metabolism
N
• in response to hypoxia (7.5% O2), adult double homozygotes display normal decreases in body temperature relative to wild-type controls (1.1 +/- 0.2 vs 1.1 +/- 0.3 degrees Celsius, respectively)





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/04/2022
MGI 6.17
The Jackson Laboratory