Mouse Genome Informatics
cn1
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Lyz2tm1(cre)Ifo/Lyz2+

B6.129P2-Lyz2tm1(cre)Ifo Nr3c1tm2Gsc
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
N
• mice exhibit normal susceptibility to MOG induced experimental autoimmune encephalomyelitis and response to treatment with dexamethasone (J:137157)


Mouse Genome Informatics
cn2
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Lck-cre)I57Jxm/0

B6.Cg-Nr3c1tm2Gsc Tg(Lck-cre)I57Jxm
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• T cells fail to exhibit an increase in apoptosis following treatment with dexamethasone unlike Nr3c1tm2Gsc control cells
• following exposure to MOG, mice exhibit earlier induction of experimental autoimmune encephalomyelitis than in similarly treated wild-type mice and fail to recover after treatment with dexamethasone as do Nr3c1tm2Gsc control mice

homeostasis/metabolism
• T cells fail to exhibit an increase in apoptosis following treatment with dexamethasone unlike Nr3c1tm2Gsc control cells
• following induction of experimental autoimmune encephalomyelitis and treatment with dexamethasone, regulatory T cell numbers are not as diminished as in Nr3c1tm2Gsc control mice and expression of Foxp3 is unaltered
• unlike in peripheral T cells from Nr3c1tm2Gsc control mice, treatment with dexamethasone does not reduced expression of cell adhesion molecules

cellular
• T cells fail to exhibit an increase in apoptosis following treatment with dexamethasone unlike Nr3c1tm2Gsc control cells

hematopoietic system
• T cells fail to exhibit an increase in apoptosis following treatment with dexamethasone unlike Nr3c1tm2Gsc control cells


Mouse Genome Informatics
cn3
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Lyz2tm1(cre)Ifo/Lyz2+

C.129P2-Lyz2tm1(cre)Ifo Nr3c1tm2Gsc
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
skeleton
N
• bone formation rate with prednisolone treatment for 2 weeks is reduced in mutants and controls (J:160669)


Mouse Genome Informatics
cn4
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Stat5atm2Mam/Stat5atm2Mam
Stat5btm1Mam/Stat5btm1Mam
Tg(Alb1-cre)7Gsc/0

involves: 129P2/OlaHsd * 129S6/SvEvTac * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
growth/size/body
• reduced body weight starting around 3 weeks of age


Mouse Genome Informatics
cn5
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Stat5btm1Mam/Stat5btm1Mam
Tg(Alb1-cre)7Gsc/0

involves: 129P2/OlaHsd * 129S6/SvEvTac * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
growth/size/body
• reduced body weight starting around 3 weeks of age


Mouse Genome Informatics
cn6
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Slc6a3-icre)1Fto/0

involves: 129P2/OlaHsd * C57BL/6 * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
behavior/neurological
N
• mutant and control mice show similar acquisition and motivation to self-administer cocaine (J:150544)

nervous system
N
• spontaneous activity (firing rates) of dopamine neurons in the ventral tegmental area (VTA) is similar to controls (J:150544)

homeostasis/metabolism
N
• circulating glucocorticoid levels are normal under basal and stress conditions (J:150544)


Mouse Genome Informatics
cn7
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Drd1a-cre)AGsc/0

involves: 129P2/OlaHsd * C57BL/6 * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
behavior/neurological
N
• Anxiety-related behavior, basic motor processes (movement, coordination), and associative learning are not impaired in mutants (J:150544)
• mice show profoundly decreased cocaine self-administration; acquistion of cocaine self-administration is similar to controls but dose-response is decreased indicating a lower reinforcing efficacy of cocaine

nervous system
• spontaneous firing rate of dopamine neurons is highly reduced in ventral tegmental area (VTA)

homeostasis/metabolism
N
• circulating glucocorticoid levels are normal under basal and stress conditions (J:150544)
• anxiety-related behaviors are not altered compared to controls indicating behavioral changes are specific to cocaine reinforcement (J:150544)


Mouse Genome Informatics
cn8
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Nes-cre)1Kln/0

involves: 129P2/OlaHsd * C57BL/6 * SJL
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
nervous system
• spontaneous firing rate of dopamine neurons is highly reduced in ventral tegmental area (VTA)


Mouse Genome Informatics
cn9
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Nes-cre)1Kln/0

involves: 129P2/OlaHsd * C57BL/6J * SJL
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
behavior/neurological
• impaired stress response in Porsolt forced-swim test; mice show normal immobility scores in an initial test, but do not show an increased response in a 24-hour restest
• in a light-dark crossing task test, mice exhibit a reduced latentcy in entering an aversive bright compartment and spent more time there
• in a zero-maze test, mice demonstrated an increased number of entries into the averse open segment and spent more time there

growth/size/body
• mice are 70+/-3% of the weight of control mice at 6-14 weeks of age
• mice are 82+/-0.4% of the length of control mice at 6-14 weeks of age

skeleton
• bone density is reduced in the skull and pelvis bones

adipose tissue
• a displacement of fat tissue towards the head and neck from the rest of the body is apparent
• mice are not obese

endocrine/exocrine glands
N
• the morphology of the adrenal glands is similar to controls (J:57315)
• levels of adrenaline and noradrenaline in the adrenal glands is similar to controls (J:57315)

homeostasis/metabolism
• morning levels of circulating glucocorticoids are elevated
• normal circadian rhythm is maintained
• plasma levels of ACTH are moderately reduced
• in the anterior pituitary, an increase in the level of ACTH and its transcript levels are seen

nervous system
• elevation of CRH peptide levels in the paraventricular nucleus of the hypothalamus (PVN)
• no change in the level of vasopression (AVP)


Mouse Genome Informatics
cn10
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Wap-cre)1Gsc/0

involves: 129P2/OlaHsd * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
endocrine/exocrine glands
• between day 14.5 of pregnancy (P14.5) and P18.5, lobuloalveolar development was retarded, however milk protein expression is normal and females are able to nurse pups although pups weight 20% less during the first 10 days after birth (J:95548)
• decreased cell proliferation in P14.5 mammary glands and increased cell proliferation in lactating (L0.5) mammary glands, however did not observe any differences in apoptosis during pregnancy (J:95548)
• although secretory alveoli form, less space is occupied by epithelial structures and more space is occupied by fat compared to controls during pregnancy and throughout lactation indicating that alveolar lobes do not completely penetrate the mammary fat pad (J:95548)

reproductive system
• between day 14.5 of pregnancy (P14.5) and P18.5, lobuloalveolar development was retarded, however milk protein expression is normal and females are able to nurse pups although pups weight 20% less during the first 10 days after birth (J:95548)
• decreased cell proliferation in P14.5 mammary glands and increased cell proliferation in lactating (L0.5) mammary glands, however did not observe any differences in apoptosis during pregnancy (J:95548)

integument
• between day 14.5 of pregnancy (P14.5) and P18.5, lobuloalveolar development was retarded, however milk protein expression is normal and females are able to nurse pups although pups weight 20% less during the first 10 days after birth (J:95548)
• decreased cell proliferation in P14.5 mammary glands and increased cell proliferation in lactating (L0.5) mammary glands, however did not observe any differences in apoptosis during pregnancy (J:95548)
• although secretory alveoli form, less space is occupied by epithelial structures and more space is occupied by fat compared to controls during pregnancy and throughout lactation indicating that alveolar lobes do not completely penetrate the mammary fat pad (J:95548)


Mouse Genome Informatics
cn11
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Camk2a-cre/ERT2)2Gsc/0

involves: 129P2/OlaHsd * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• when treated with tamoxifen the level of serum corticosterone is increased to 39+/-8 ng/ml when one copies of the transgene is present, 100+/-20 ng/ml when two copies of the transgene are present and 108+/-19 ng/ml when four copies of the transgene are present compared to 13+/-5 ng/ml in wild-type


Mouse Genome Informatics
cn12
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Runx2-icre)1Jtuc/0

involves: 129P2/OlaHsd * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
growth/size/body
• males exhibit a slight increase in crown-rump length compared to controls at 10 weeks

skeleton
N
• bone resorption is slightly decreased in both mutants and controls while osteoclast numbers are not affected (J:160669)
• calvarial osteoblasts exhibit diminished differentiation potential in terms of bone nodule formation and alkaline phosphatase expression
• with prednisolone treatment, osteoblast differentiation is impaired in wild-type mice, but is unaffected in mutants, as determined by Col1a1 expression, marking functional osteoblasts
• in response to two weeks of treatment with the Nr3c1 agonist prednisolone, mutants do not display reduced bone mineral density in vertebral bones in contrast to treated controls
• mice show modest but highly significant decrease in bone density in calcified vertebral sections compared to controls at 3 months
• trabecular numbers are decreased but trabecular thickness is unaltered resulting in increased trabecular spacing
• osteoblast numbers, osteoblast surface, osteocytes, and osteoclast parameters are not significantly changed compared to controls
• bone formation in vertebrae is not inhibited whereas treated controls show near complete inhibition of bone formation with prednisolone treatment; osteoblastogenesis (numbers of colony forming units of osteoblasts) is not impaired as seen in controls with prednisolone treatment

cellular
• calvarial osteoblasts exhibit diminished differentiation potential in terms of bone nodule formation and alkaline phosphatase expression
• with prednisolone treatment, osteoblast differentiation is impaired in wild-type mice, but is unaffected in mutants, as determined by Col1a1 expression, marking functional osteoblasts


Mouse Genome Informatics
cn13
    Nr3c1tm2Gsc/Nr3c1tm2Gsc
Tg(Alb1-cre)7Gsc/0

involves: 129P2/OlaHsd * FVB/N
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
growth/size/body
• reduced body weight starting around 3 weeks of age