Phenotypes associated with this allele

• Allele Symbol: Foxf1^tm1Pca
• Allele Name: targeted mutation 1, Peter Carlsson
• Allele ID: MGI:1929432
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Foxf1^tm1Pca/Foxf1^tm1Pca	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CD-1) or (involves: 129X1/SvJ * C57BL/6)	MGI:2171425
hm2	Foxf1^tm1Pca/Foxf1^tm1Pca	involves: 129P2/OlaHsd * C57BL/6	MGI:3818881

Genotype
MGI:2171425

hm1

• Allelic Composition: Foxf1^tm1Pca/Foxf1^tm1Pca
• Genetic Background: either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CD-1) or (involves: 129X1/SvJ * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:66591 )

• embryos die at midgestation, between E9.5 and E10

embryo

abnormal developmental patterning ( J:66591 )

• the posterior part of the embryo is developmentally retarded compared with the anterior part

failure of initiation of embryo turning ( J:66591 )

• embryos do not turn

abnormal embryonic tissue morphology ( J:66591 )

• embryos are severely disfigured at E9.5

abnormal lateral plate mesoderm morphology ( J:66591 )

• impaired lateral plate differentiation and coelom formation

• incomplete separation of splanchnic and somatic mesoderm

abnormal posterior primitive streak morphology ( J:66591 )

• mutants exhibit a lower proliferation rate of mesodermal cells in the posterior primitive streak, resulting in an underdeveloped posterior end

abnormal somite development ( J:66591 )

• somites become asymmetrically arranged on either side of the neural tube

delayed somite formation ( J:66591 )

• somite formation is retarded such that fewer somites are seen at E8.5

abnormal extraembryonic tissue morphology ( J:66591 )

absent vitelline blood vessels ( J:66591 )

• vascular plexus does not form

abnormal allantois morphology ( J:66591 )

• although the allantois grows, it retains a primitive, bud-like shape, does not elongate and fails to contact the chorion

• defective mesodermal expansion and lack of vascularization of the allantois

small allantois ( J:66591 )

• allantois does not elongate

abnormal amnion morphology ( J:66591 )

• amnion is small, but thicker, consisting of multiple layers of rounded cells instead of the normal flattened bilayer, and does not expand with the growth of the embryo

• impaired differentiation of amniotic mesoderm; expression analysis indicates that the amniotic mesoderm resembles yolk sac mesoderm

• ectopic hematopoiesis in the amnion

abnormal visceral yolk sac morphology ( J:66591 )

• yolk sacs at E9.5 are wrinkled, shrunken, anemic and lack vasculature

• endodermal and mesodermal layers of the yolk sac are separated from each other, first seen at E8.5

abnormal extraembryonic mesoderm development ( J:66591 )

• defects in extraembryonic mesoderm differentiation

• mesodermal layers of the amnion and yolk sack frequently adhere to each other, indicating enhanced intramesodermal cohesion, and as the yolk sac mesoderm separates from the endoderm, it occasionally fuses with the amnion

• yolk sac and amniotic mesoderm are unable to expand

failure of chorioallantoic fusion ( J:66591 )

• no placentation due to the lack of chorioallantoic fusion

digestive/alimentary system

abnormal hindgut morphology ( J:66591 )

• formation of the involuted hindgut diverticulum is delayed and the posterior visceral endoderm is flat and enclosed in a wide yolk-sac pocket

absent midgut ( J:66591 )

• midgut does not form because embryos fail to turn

cardiovascular system

absent vitelline blood vessels ( J:66591 )

• vascular plexus does not form

Genotype
MGI:3818881

hm2

• Allelic Composition: Foxf1^tm1Pca/Foxf1^tm1Pca
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

embryo

absent vitelline blood vessels ( J:128367 )

• yolk sacs are completely avascular

abnormal visceral yolk sac morphology ( J:128367 )

• at E9.5, yolk sac surface has wrinkles which are folds in the visceral endoderm which are prevented from expanding by constricted mesodermal layer

abnormal visceral yolk sac mesenchyme morphology ( J:128367 )

• yolk sac mesoderm has abnormal adhesion properties

• much of yolk sac mesoderm is detached and forms thick layer between yolk sac and amnion

• amnion appears normal but yolk sac mesoderm is hypoplastic and is reduced to thin lining of the endoderm

cardiovascular system

absent vitelline blood vessels ( J:128367 )

• yolk sacs are completely avascular