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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Nkx3-1tm1Hha
targeted mutation 1, Hans-Henning Arnold
MGI:1928654
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Nkx3-1tm1Hha/Nkx3-1tm1Hha involves: 129S4/SvJae * C57BL/6 MGI:2175150
ht2
Nkx3-1tm1Hha/Nkx3-1+ involves: 129S4/SvJae * C57BL/6 MGI:2175151
cx3
Nkx3-2tm1Bobh/Nkx3-2tm1Bobh
Nkx3-1tm1Hha/Nkx3-1tm1Hha
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 MGI:3608887


Genotype
MGI:2175150
hm1
Allelic
Composition
Nkx3-1tm1Hha/Nkx3-1tm1Hha
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx3-1tm1Hha mutation (0 available); any Nkx3-1 mutation (20 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
• homozygotes are developmentally normal and fertile; however, mutant males display inadequate plug formation upon copulation with advancing age

endocrine/exocrine glands
• homozygotes display a marked size reduction in all three minor salivary glands, with smaller apical mucous end-pieces relative to wild-type mice
• the mutant palatine gland is significantly smaller; similar size reductions are noted in the glandula lingualis and glandula buccalis
• in addition, the ductal system exhibits irregular branching esp. in the glandula palatina and glandula lingualis
• in homozygotes, the ducts of minor salivary glands appear smaller in diameter and the diameter of the ductal lumen is reduced by thicker epithelium
• smaller ducts with reduced internal lumens are particularly pronounced in the palatine and lingual glands
• adult homozygotes show evidence for salivary gland epithelial hyperplasia, although not as severe as in the prostate
• homozygotes exhibit significantly smaller minor salivary glands (i.e. glandula buccalis, glandula palatina, and glandula lingualis) relative to wild-type mice
• unlike wild-type mice, homozygotes frequently contain relatively dry food residues in their mouths, suggesting impaired mucous salivary production
• at 4 months, mutant BUGs exhibit an increased number of nuclei, suggesting hyperproliferation of epithelial cells
• at 4 months, mutant BUGs show a dramatic reduction of mucin-producing cells and contain primarily ductal cells, suggesting abnormal cell differentiation or an imbalance in cell growth
• at 3 months, homozygotes exhibit a significantly smaller bulbourethral glands (BUGs) relative to wild-type mice
• homozygotes invariably display prostate epithelial hyperplasia in the absence of overt prostate tumors during the observation period of >1 year
• at 3 months, homozygotes exhibit a moderate size reduction of the anterior prostate
• at 3 months, homozygotes exhibit fewer but enlarged prostatic ducts in the anterior prostate and other prostatic lobes
• although adult homozygotes exhibit all three prostatic lobes, the number of prostatic ducts appears reduced relative to heterozygous and wild-type mice
• in addition, individual ducts appear enlarged, suggesting defective ductal branching
• adult homozygotes show progressive dysplastic changes in the duct epithelium of the anterior and dorsolateral prostate, resulting in a severely dysplastic, multi-layered epithelium with little secreted fluid in the duct lumen at 10 months
• as early as 2 months, homozygotes exhibit progressive prostate epithelial hyperplasia in the anterior and dorsolateral lobes; however, no hyperplasia is noted in ventral prostatic lobes

reproductive system
• at 4 months, mutant BUGs exhibit an increased number of nuclei, suggesting hyperproliferation of epithelial cells
• at 4 months, mutant BUGs show a dramatic reduction of mucin-producing cells and contain primarily ductal cells, suggesting abnormal cell differentiation or an imbalance in cell growth
• at 3 months, homozygotes exhibit a significantly smaller bulbourethral glands (BUGs) relative to wild-type mice
• homozygotes invariably display prostate epithelial hyperplasia in the absence of overt prostate tumors during the observation period of >1 year
• at 3 months, homozygotes exhibit a moderate size reduction of the anterior prostate
• at 3 months, homozygotes exhibit fewer but enlarged prostatic ducts in the anterior prostate and other prostatic lobes
• although adult homozygotes exhibit all three prostatic lobes, the number of prostatic ducts appears reduced relative to heterozygous and wild-type mice
• in addition, individual ducts appear enlarged, suggesting defective ductal branching
• adult homozygotes show progressive dysplastic changes in the duct epithelium of the anterior and dorsolateral prostate, resulting in a severely dysplastic, multi-layered epithelium with little secreted fluid in the duct lumen at 10 months
• as early as 2 months, homozygotes exhibit progressive prostate epithelial hyperplasia in the anterior and dorsolateral lobes; however, no hyperplasia is noted in ventral prostatic lobes

digestive/alimentary system
• homozygotes display a marked size reduction in all three minor salivary glands, with smaller apical mucous end-pieces relative to wild-type mice
• the mutant palatine gland is significantly smaller; similar size reductions are noted in the glandula lingualis and glandula buccalis
• in addition, the ductal system exhibits irregular branching esp. in the glandula palatina and glandula lingualis
• in homozygotes, the ducts of minor salivary glands appear smaller in diameter and the diameter of the ductal lumen is reduced by thicker epithelium
• smaller ducts with reduced internal lumens are particularly pronounced in the palatine and lingual glands
• adult homozygotes show evidence for salivary gland epithelial hyperplasia, although not as severe as in the prostate
• homozygotes exhibit significantly smaller minor salivary glands (i.e. glandula buccalis, glandula palatina, and glandula lingualis) relative to wild-type mice
• unlike wild-type mice, homozygotes frequently contain relatively dry food residues in their mouths, suggesting impaired mucous salivary production

cellular
• in mutant glandula palatina and glandula lingualis, the epithelium appears significantly thicker, suggesting epithelial hyperproliferation

embryo
N
• surpisingly, homozygotes exhibit no sclerotomal defects and develop into adulthood with no apparent skeletal abnormalities

homeostasis/metabolism
• unlike wild-type mice, homozygotes frequently contain relatively dry food residues in their mouths, suggesting impaired mucous salivary production




Genotype
MGI:2175151
ht2
Allelic
Composition
Nkx3-1tm1Hha/Nkx3-1+
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx3-1tm1Hha mutation (0 available); any Nkx3-1 mutation (20 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• at 10 months, heterozygotes exhibit dysplastic changes in the anterior prostate, although of reduced severity relative to homozygotes
• at 2 months, heterozygotes exhibit a normal ductal epithelium in the anterior prostate (AP) relative to wild-type mice; however, moderate AP hyperplasia is observed at 10 months
• by 10 months, heterozygotes also exhibit epithelial hyperplasia in the dorsolateral prostate, although of reduced severity relative to homozygotes
• similar to homozygotes, no hyperplasia is observed in heterozygous ventral prostatic lobes

endocrine/exocrine glands
• at 10 months, heterozygotes exhibit dysplastic changes in the anterior prostate, although of reduced severity relative to homozygotes
• at 2 months, heterozygotes exhibit a normal ductal epithelium in the anterior prostate (AP) relative to wild-type mice; however, moderate AP hyperplasia is observed at 10 months
• by 10 months, heterozygotes also exhibit epithelial hyperplasia in the dorsolateral prostate, although of reduced severity relative to homozygotes
• similar to homozygotes, no hyperplasia is observed in heterozygous ventral prostatic lobes




Genotype
MGI:3608887
cx3
Allelic
Composition
Nkx3-2tm1Bobh/Nkx3-2tm1Bobh
Nkx3-1tm1Hha/Nkx3-1tm1Hha
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nkx3-1tm1Hha mutation (0 available); any Nkx3-1 mutation (20 available)
Nkx3-2tm1Bobh mutation (0 available); any Nkx3-2 mutation (19 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• at E12.5, double homozygotes are present at the expected Mendelian frequency
• however, only ~50% and 15% of the expected number are obtained at E14.5 and E17.5, respectively, with a gradually increasing embryo loss noted between E13.5 and E17.5
• no double homozygotes are recovered alive after E17.5

skeleton
• at E17.5, the axial skeleton of double homozygotes appears even shorter than that of Bapx1tm1Bobh homozygotes
• at E17.5, double homozygotes exhibit even more tightly spaced ribs than Bapx1tm1Bobh homozygotes
• at E17.5, the double mutant atlas is reduced to only the lateral parts lacking most of the cartilage that gives rise to the vertebral body
• at E17.5, double mutant lumbar vertebrae completely lack the normal dorsal processes observed as bifurcations in Bapx1tm1Bobh homozygotes
• at E17.5, double homozygotes show an enhanced reduction or loss of ventro-medial cartilaginous material in all cervical vertebrae relative to Bapx1tm1Bobh homozygotes
• at E17.5, double homozygotes lack the central ossification centers in cervical vertebrae, with the atlas being most severely affected; this defect is less obvious in thoracic and lumbar vertebrae
• at E14.5, double homozygotes exhibit a severe reduction in the number of chondrogenic cells around the notochord, with only few scattered cells present at the cervical level but no signs of cartilage formation; at lumbar level, chondrogenic cell numbers are also further reduced relative to Bapx1tm1Bobh homozygotes
• except for the region of pedicles essentially no cartilage is formed in the vertebral anlagen of double homozygotes
• double homozygotes exhibit exacerbated sclerotomal defects in cervical and lumbar segments relative to Bapx1tm1Bobh homozygotes

limbs/digits/tail
• at E17.5, double homozygotes display severely kinked tails relative to Bapx1tm1Bobh homozygotes





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last database update
08/06/2019
MGI 6.14
The Jackson Laboratory