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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Cd74tm1Liz
targeted mutation 1, Elizabeth K Bikoff
MGI:1927530
Summary 15 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Cd74tm1Liz/Cd74tm1Liz B6.129S-Cd74tm1Liz MGI:3688838
hm2
Cd74tm1Liz/Cd74tm1Liz B6.129S-Cd74tm1Liz/J MGI:5491939
hm3
Cd74tm1Liz/Cd74tm1Liz involves: 129S/SvEv MGI:3760861
hm4
Cd74tm1Liz/Cd74tm1Liz involves: 129S/SvEv * BALB/cAn MGI:3689073
hm5
Cd74tm1Liz/Cd74tm1Liz involves: 129S/SvEv * BALB/c * C57BL MGI:3689074
hm6
Cd74tm1Liz/Cd74tm1Liz involves: 129S/SvEv * C57BL/6 MGI:3689001
hm7
Cd74tm1Liz/Cd74tm1Liz involves: 129S/SvEv * C57BL/6J MGI:3688756
hm8
Cd74tm1Liz/Cd74tm1Liz involves: 129S/SvEv * C57BL * C57BL/10 * C57BR MGI:3689075
ht9
Cd74tm1Liz/Cd74+ involves: 129S/SvEv MGI:4421700
ht10
Cd74tm2Liz/Cd74tm1Liz involves: 129S/SvEv * C57BL/6 MGI:3688837
cx11
Cd74tm1Liz/Cd74tm1Liz
Sppl2atm1Basc/Sppl2atm1Basc
B6.129-Sppl2atm1Basc Cd74tm1Liz MGI:5491938
cx12
Cd74tm1Liz/Cd74tm1Liz
Sppl2atm1Dgen/Sppl2atm1Dgen
involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6 MGI:5484587
cx13
Cd74tm1Liz/Cd74tm1Liz
H2-DMatm1Luc/H2-DMatm1Luc
Tg(CD74)AR194Ayr/?
involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J MGI:3760430
cx14
Cd74tm1Liz/Cd74tm1Liz
H2-DMatm1Luc/H2-DMatm1Luc
Tg(CD74*)1Ayr/0
involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J MGI:3839583
cx15
Cd74tm1Liz/Cd74tm1Liz
H2-DMatm1Liz/H2-DMatm1Liz
involves: 129S/SvEv * BALB/c MGI:3689121


Genotype
MGI:3688838
hm1
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
B6.129S-Cd74tm1Liz
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type

immune system
• lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type
• significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type
• in vivo, Th1 response to MOG is absent unlike in wild-type mice
• in vitro, lymph node cells from mice receiving wild-type MOG T cells exhibit reduced Th1 recall response compared with similarly treated wild-type mice
• in vivo, Th1 response to PLP is reduced compared to in wild-type mice
• in vitro, the frequency of Th1 recall response is greater than in H2-Dmatm1Luc homozygotes
• dramatically compared to in wild-type mice when wild-type MOG T cells are transferred
• antigen presenting cells cannot process and present recombinant MOG protein unlike wild-type cells
• class II molecules are relatively ineffective for presentation of native protein antigens
• mutants expressed greatly reduced amounts of class II on spleen cells compared to wild-type
• in spleen cells, immature alpha and beta chains exhibiting no evidence of having been exported past the cis-Golgi are observed, whereas compact alpha/beta dimers efficiently transported past the cis-Golgi are produced by Cd74tm2Liz-deficient cells; floppy conformers are seen, compared to compact dimers in wild-type
• whether MOG-primed or adoptively transferred with MOG-specific T blasts, mice fail to develop symptoms of experimental autoimmune encephalomyelitis (EAE) unlike wild-type mice
• PLP-treated mice fail to develop EAE unlike similarly treated wild-type mice

hematopoietic system
• lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type
• significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type
• in vivo, Th1 response to MOG is absent unlike in wild-type mice
• in vitro, lymph node cells from mice receiving wild-type MOG T cells exhibit reduced Th1 recall response compared with similarly treated wild-type mice
• in vivo, Th1 response to PLP is reduced compared to in wild-type mice
• in vitro, the frequency of Th1 recall response is greater than in H2-Dmatm1Luc homozygotes




Genotype
MGI:5491939
hm2
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
B6.129S-Cd74tm1Liz/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• in the spleen and lymph node

hematopoietic system
• in the spleen and lymph node




Genotype
MGI:3760861
hm3
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
involves: 129S/SvEv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
N
• no evidence for anemia or thrombocytopenia
• CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow

immune system
• CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow




Genotype
MGI:3689073
hm4
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
involves: 129S/SvEv * BALB/cAn
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• B cell maturation is defective
• higher percentages of immature B cells lacking surface IgD and CD23 expression
• mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold (J:110927)
• T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens (J:113714)
• splenocytes display enhanced binding capabilities
• dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
• mutant spleen cells on an H-2d background show enhanced peptide-loading capabilities over wild-type cells (J:110927)
• T cell hybridomas produce Il-2 and T cell lines proliferate when stimulated by mutant spleen cells; similar results are seen using T cell clones specific for peptides including ovalbumin (OVA)323-339 and IgG2ab (J:110927)
• spleen cells are ineffective for presentation of intact protein antigen as opposed to processed peptide antigens (J:110927)
• spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses (J:110927)
• antigen presentation by mutant spleen cells to Edrestricted T cell clones is substantially decreased (J:113714)
• Edrestricted T cells show enhanced activity when challenged with endogenously process beta 2 microglobulin (B2m) epitopes in presence of mutant splenocytes (J:113714)

hematopoietic system
• B cell maturation is defective
• higher percentages of immature B cells lacking surface IgD and CD23 expression
• mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold (J:110927)
• T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens (J:113714)




Genotype
MGI:3689074
hm5
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
involves: 129S/SvEv * BALB/c * C57BL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL46-61 peptide show similar response to wild-type mice

immune system
• proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL46-61 peptide show similar response to wild-type mice
• B cell maturation is defective
• higher percentages of immature B cells lacking surface IgD and CD23 expression
• dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
• spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses

hematopoietic system
• proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL46-61 peptide show similar response to wild-type mice
• B cell maturation is defective
• higher percentages of immature B cells lacking surface IgD and CD23 expression




Genotype
MGI:3689001
hm6
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mutants display isotype-specific antigen presentation defects; MHC Ak-restricted antigen presentation is reduced compared to wild-type




Genotype
MGI:3688756
hm7
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
involves: 129S/SvEv * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells

growth/size/body
• homozygotes are usually smaller than littermates
• occasionally, homozygotes display symptoms of severe wasting

hematopoietic system
• immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells
• mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus
• number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells

immune system
• immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells
• mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus
• number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells
• splenic antigen presenting cells (APCs) present an encephalitogenic MOG peptide p35-55 to I-Ab-restricted p35-55-specific T cells equivalently to wild-type APCs, stimulating proliferation but are ineffective at presenting intact MOG to T cells compared with wild-type APCs, suggesting that processing is required for presentation of intact MOG
• mutant spleen cells stimulated stronger responses in presence of already processed peptides hen egg lysozyme (HEL) 74-88 and Ealpha 52-68, but fail to present native protein antigens keyhole limpet cyanin (KLH) and IgG2a
• mutant spleen cells exhibit reduced levels of I-Ab surface antigen compared to wild-type spleen cells
• mutant spleen cells show significantly reduced amounts of alpha/beta heterodimers associated with the invariant chain; increased amounts of free alpha and beta chains are present
• majority of class II molecules are comprised of immature alpha and beta chains that have not been exported past the cis-Golgi
• no mature compact SDS-resistant alpha/beta heterodimers (I-Ab) are produced by mutant spleens in contrast to wild-type cells
• class II molecules produced by mutants show enhanced peptide binding compared to wild-type; molecules behave as if empty or occupied by easily displaced peptide in contrast to wild-type cells that are stably occupied with self and serum-derived peptides
• immunization with p35-55 does not induce Ifng secretion indicating inability to prime p35-55 specific CD4+ T cells
• mice do not develop experimental autoimmune encephalitis (EAE) upon direct immunization with p35-55 while wild-type mice do develop EAE
• adoptive transfer of wild-type encephalitogenic T cells does not induce EAE in mutant recipients




Genotype
MGI:3689075
hm8
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Genetic
Background
involves: 129S/SvEv * C57BL * C57BL/10 * C57BR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA323-339 peptide show similar response to wild-type mice

immune system
• proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA323-339 peptide show similar response to wild-type mice
• B cell maturation is defective
• higher percentages of immature B cells lacking surface IgD and CD23 expression
• dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
• spleen cells from mice with an H-2k haplotype background effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses

hematopoietic system
• proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA323-339 peptide show similar response to wild-type mice
• B cell maturation is defective
• higher percentages of immature B cells lacking surface IgD and CD23 expression




Genotype
MGI:4421700
ht9
Allelic
Composition
Cd74tm1Liz/Cd74+
Genetic
Background
involves: 129S/SvEv
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
N
• no evidence for anemia or thrombocytopenia




Genotype
MGI:3688837
ht10
Allelic
Composition
Cd74tm2Liz/Cd74tm1Liz
Genetic
Background
involves: 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
Cd74tm2Liz mutation (1 available); any Cd74 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• lymph node cells show equivalent proliferative responses to KLH immunization compared to those from heterozygous littermates, but greater than wild-type cells

immune system
N
• compact AalphabAbetab dimers are produced efficiently in absence of invariant chain p41 isoform
• mutants show no significant differences in level of class II expressed on spleen cells compared to wild-type
• normal numbers of CD4+CD8- T cells are observed compared to Cd74tm1Liz
• lymph node cells show equivalent proliferative responses to KLH immunization compared to those from heterozygous littermates, but greater than wild-type cells
• cell expressing the p31 invariant chain (lacking p41 chain) can present native protein antigens including keyhole limpet cyanin (KLH), ovalbumin (OVA), and IgG2aa compared to complete invariant chain deficient spleen cells

hematopoietic system
• lymph node cells show equivalent proliferative responses to KLH immunization compared to those from heterozygous littermates, but greater than wild-type cells




Genotype
MGI:5491938
cx11
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Sppl2atm1Basc/Sppl2atm1Basc
Genetic
Background
B6.129-Sppl2atm1Basc Cd74tm1Liz
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
Sppl2atm1Basc mutation (0 available); any Sppl2a mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Sppl2atm1Basc/Sppl2atm1Basc Cd74tm1Liz/Cd74tm1Liz B cells show normal morphology compared to the increased vacuoles in Sppl2atm1Basc/Sppl2atm1Basc B cells

immune system
N
• B cells exhibit the same number of vacuoles per cells as wild-type mice and Cd74tm1Liz homozygotes
• calcium ion flux in T1 B cells is restored to normal
• B cell maturation and function are restored
• the size of Peyer's patches is restored
• compared with wild-type mice but improved from Sppl2atm1Basc homozygotes in the spleen and lymph nodes
• compared with wild-type mice but improved from Sppl2atm1Basc homozygotes

hematopoietic system
• compared with wild-type mice but improved from Sppl2atm1Basc homozygotes in the spleen and lymph nodes




Genotype
MGI:5484587
cx12
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
Sppl2atm1Dgen/Sppl2atm1Dgen
Genetic
Background
involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
Sppl2atm1Dgen mutation (0 available); any Sppl2a mutation (26 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
N
• mice exhibit normalized numbers of T2 B cells, marginal zone B cells and myeloid dendritic cells

hematopoietic system




Genotype
MGI:3760430
cx13
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
H2-DMatm1Luc/H2-DMatm1Luc
Tg(CD74)AR194Ayr/?
Genetic
Background
involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
H2-DMatm1Luc mutation (1 available); any H2-DMa mutation (19 available)
Tg(CD74)AR194Ayr mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• the number of CD4+ T cells is reduced to levels below wild-type but similar to those observed in Cd74tm1Liz H2-DMatm1Luc homozygotes
• unlike in wild-type cells, splenocytes are unable to elicit presentation of MHC class II exogenous antigens or of two endogenous antigens (IgM and beta-2 microglobulin)
• however, Tg(CD74)AR194Ayr completely rescues the MHC class II peptide loading observed in Cd74tm1Liz H2-DMatm1Luc homozygotes and presentation of endogenous antigens for CD22 is normal
• after incubation with Ealpha peptide, loading of MHC class II is less than on similarly treated Cd74tm1Liz H2-DMatm1Luc Tg(CD74*)AR194Ayr splenocytes

hematopoietic system
• the number of CD4+ T cells is reduced to levels below wild-type but similar to those observed in Cd74tm1Liz H2-DMatm1Luc homozygotes




Genotype
MGI:3839583
cx14
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
H2-DMatm1Luc/H2-DMatm1Luc
Tg(CD74*)1Ayr/0
Genetic
Background
involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
H2-DMatm1Luc mutation (1 available); any H2-DMa mutation (19 available)
Tg(CD74*)1Ayr mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• the number of CD4+ T cells is rescued to 60% of normal
• Tg(CD74)AR194Ayr partially rescues the MHC class II peptide loading observed in Cd74tm1Liz H2-DMatm1Luc homozygotes
• after incubation with Ealpha peptide, loading of MHC class II is greater than on similarly treated Cd74tm1Liz H2-DMatm1Luc Tg(CD74)AR194Ayr splenocytes
• splenocytes elicit greater antigen presentations than in Cd74tm1Liz H2-DMatm1Luc Tg(CD74)AR194Ayr splenocytes
• splenocytes elicit presentation of exogenous protein antigens derived from one but not two antigen
• splenocyte presentaton of IgM and beta-2 microglobulin epitpes is less efficient than in Cd74tm1Liz Tg(CD74)AR194Ayr or Cd74tm1Liz Tg(CD74*)AR194Ayr mice
• however, splenocytes can elicit presentation from two endogenous protein antigens (IgM and beta-2 microglobulin)

hematopoietic system
• the number of CD4+ T cells is rescued to 60% of normal




Genotype
MGI:3689121
cx15
Allelic
Composition
Cd74tm1Liz/Cd74tm1Liz
H2-DMatm1Liz/H2-DMatm1Liz
Genetic
Background
involves: 129S/SvEv * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (3 available); any Cd74 mutation (24 available)
H2-DMatm1Liz mutation (0 available); any H2-DMa mutation (19 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• double mutants display significant defects in CD4+ T cell development; numbers of CD4+ T cells in the thymus and periphery are greatly reduced compared to wild-type

hematopoietic system
• double mutants display significant defects in CD4+ T cell development; numbers of CD4+ T cells in the thymus and periphery are greatly reduced compared to wild-type





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory