Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
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immune system
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• spleen and lymph node cells produce an average of 5-fold higher levels of IL-3
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• spleen and lymph node cells produce IFN gamma at comparable levels to wild-type at 8 weeks after parasite infection
• no IL-4 was detected at any time
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• spleen and lymph node cells produce an average of 3.7-fold higher levels of LT-TNF
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• lesions after infection progress similarly to those in wild-type up to 7 weeks post-infection, then decrease slightly in size and remain a constant size over a 23-week period
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nervous system
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• area of the ipsilateral projection is increased compared to controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
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homeostasis/metabolism
hematopoietic system
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• in the spleen and thymus
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immune system
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• in the spleen and thymus
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
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hematopoietic system
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• decreased proportion of CD8+ alpha-beta+ T cells
(J:744)
• 100-150-fold reduction in T-cell antigen receptor alpha-beta+ CD4-CD8+ T cells in adult thymus and spleen, however levels of gamma-delta T cells is normal
(J:80910)
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• frequency of gamma-delta+ T cells was considerably higher in the thymus (11% vs. 6.5% of CD4-CD8- thymocytes), however development and functional competence of gamma-delta+ T cells was normal
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• increased frequency of gamma-delta+ T cells in the intestinal epithelium
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• spleen cells did not contain cytotoxic T lymphocyte precursors
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• spleen cells fail to generate allo-specific cytotoxic T lympocyte response when stimulated with BALB/c (H-2d) spleen cells
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immune system
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• decreased proportion of CD8+ alpha-beta+ T cells
(J:744)
• 100-150-fold reduction in T-cell antigen receptor alpha-beta+ CD4-CD8+ T cells in adult thymus and spleen, however levels of gamma-delta T cells is normal
(J:80910)
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• frequency of gamma-delta+ T cells was considerably higher in the thymus (11% vs. 6.5% of CD4-CD8- thymocytes), however development and functional competence of gamma-delta+ T cells was normal
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• increased frequency of gamma-delta+ T cells in the intestinal epithelium
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• spleen cells did not contain cytotoxic T lymphocyte precursors
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• spleen cells fail to generate allo-specific cytotoxic T lympocyte response when stimulated with BALB/c (H-2d) spleen cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
Tg(B2m)1Rms mutation
(0 available)
Tg(INS-cre)2Rms mutation
(1 available)
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nervous system
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• 52% of P13 mice and 57% of adult mice have enlarged lateral ventricles
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• area of the ipsilateral projection is increased compared to controls
• in some cases, multiple ectopic clusters of inputs are present in the medial areas of the LGN
• retinogeniculate axons, glomeruli and bouton appear normal
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• increase in size of excitatory presynaptic boutons
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• mice exhibit significantly fewer perforations at hippocampal synapses that wild-type
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• presynaptic nerve terminals contain 10% more synaptic vesicles than wild-type
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• LTP in response to tetanus is enhanced by approximately 227% as compared to wild type
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• 40% increase in frequency in hippocampal neurons
• 100% increase in frequency in layer 4 cortical neurons
• median amplitude is similar to wild-type in hippocampal and layer 4 neurons
• treatment with the neural activity blocker, Tetrodotoxin (TTX), does not increase mEPSC in mutant hippocampal cultures in contrast to increases in both amplitude and frequency observed in wild-type
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• 900 pulses at 1 Hz enhances baseline LTD, but LTD is unchanged in wild-type
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• 900 pulses at 0.5 Hz does not induce significant LTD in contrast to wild-type response
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
Themistm1Lov mutation
(0 available);
any
Themis mutation
(55 available)
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immune system
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• CD3hi CD8 single-positive thymocytes are present in these mice
• these thymocytes are TCRhiCD69+ which indicates that they received positively selecting signals by interaction with self peptide-MHC class II complexes but are subsequently 'redirected' to the CD8 lineage
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hematopoietic system
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• CD3hi CD8 single-positive thymocytes are present in these mice
• these thymocytes are TCRhiCD69+ which indicates that they received positively selecting signals by interaction with self peptide-MHC class II complexes but are subsequently 'redirected' to the CD8 lineage
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
Rr96tm1.1Yzo mutation
(0 available);
any
Rr96 mutation
(0 available)
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immune system
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• the peripheral CD4+CD8+ double positive T cells observed in Cd4tm1.1Yzo homozygotes are absent in these mice demonstrating Class I-dependence of this cell population
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hematopoietic system
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• the peripheral CD4+CD8+ double positive T cells observed in Cd4tm1.1Yzo homozygotes are absent in these mice demonstrating Class I-dependence of this cell population
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
Rr94tm3.2Litt mutation
(0 available);
any
Rr94 mutation
(0 available)
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immune system
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• thymocytes exhibit lymphopenia-induced homeostatic proliferation in the periphery unlike in wild-type mice
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• the frequency of TCRbetahi thymocyte is decreased 10-fold compared to in wild-type mice
• mice exhibit peripheral TCRbeta<+> T cells that do not express CD4 or CD8 unlike in wild-type mice
• mice exhibit a reduction in mature thymocyte output compared with wild-type mice
• however, the expression of CD4 in post-selection thymocytes is normal
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• the ratio of CD4 single positive to CD8 single positive T cells is inverted compared to in wild-type mice
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hematopoietic system
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• thymocytes exhibit lymphopenia-induced homeostatic proliferation in the periphery unlike in wild-type mice
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• the frequency of TCRbetahi thymocyte is decreased 10-fold compared to in wild-type mice
• mice exhibit peripheral TCRbeta<+> T cells that do not express CD4 or CD8 unlike in wild-type mice
• mice exhibit a reduction in mature thymocyte output compared with wild-type mice
• however, the expression of CD4 in post-selection thymocytes is normal
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• the ratio of CD4 single positive to CD8 single positive T cells is inverted compared to in wild-type mice
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cellular
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• thymocytes exhibit lymphopenia-induced homeostatic proliferation in the periphery unlike in wild-type mice
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growth/size/body
immune system
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• plasma cell hyperplasia within the spleen and lymph nodes
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• greater percentage of CD45RBdullCD4+ T cells than in homozygous B2m mice
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• increased amounts of IL-4, IL-6, and IL-10 and decreased amounts of IL-2 in Con A stimulated day 16 splenocytes
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• decreased amount of TNF-alpha in Con A stimulated day 16 splenocytes
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• complete disruption of normal architecture, with abundant lymphoblasts and reticular cell hyperplasia
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hematopoietic system
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• plasma cell hyperplasia within the spleen and lymph nodes
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• greater percentage of CD45RBdullCD4+ T cells than in homozygous B2m mice
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homeostasis/metabolism
integument
immune system
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• single-positive CD8+ cells are found among mature thymocytes compared to mice null for just the B2m locus that have no mature CD8+ thymocytes
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• an unusual double-positive thymocyte population exists that has surface markers of mature single positive thymocytes (HSAlo-neg TCRbetahi) and may represent single-positive CD4+ thymoyctes that are redirected to a CD8+ T cell lineage
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hematopoietic system
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• single-positive CD8+ cells are found among mature thymocytes compared to mice null for just the B2m locus that have no mature CD8+ thymocytes
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• an unusual double-positive thymocyte population exists that has surface markers of mature single positive thymocytes (HSAlo-neg TCRbetahi) and may represent single-positive CD4+ thymoyctes that are redirected to a CD8+ T cell lineage
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
Cd4tm1Jrp mutation
(0 available);
any
Cd4 mutation
(82 available)
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hematopoietic system
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• an increase in CD8+ T cells similar to that seen in single Cd4tm1Jrp mutants
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immune system
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• an increase in CD8+ T cells similar to that seen in single Cd4tm1Jrp mutants
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immune system
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• mice have 2-fold more mature single-positive CD8 thymocytes than wild-type mice and over a 1000-fold more than fold in B2mtm1Jae homozygote controls
• in the periphery, CD8+ T cells make up 2% of the lymph node population compared to less than 0.1% for B2mtm1Jae homozygote controls
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• lymph node cellularity is 25% that of controls, with a normal ratio between CD4+ and CD8+ T cells
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hematopoietic system
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• mice have 2-fold more mature single-positive CD8 thymocytes than wild-type mice and over a 1000-fold more than fold in B2mtm1Jae homozygote controls
• in the periphery, CD8+ T cells make up 2% of the lymph node population compared to less than 0.1% for B2mtm1Jae homozygote controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
Tg(H2-K-Cd86)27All mutation
(0 available)
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hematopoietic system
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• no B cells are detected in blood of B2m-deficient transgenic mice
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immune system
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• no B cells are detected in blood of B2m-deficient transgenic mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Jae mutation
(8 available);
any
B2m mutation
(122 available)
Toxtm1.1Kay mutation
(0 available);
any
Tox mutation
(37 available)
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immune system
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• about twice as much CD8+ T cells are found in the thymus of double knockouts compared to mice homozygote for just the B2mtm1Jae allele
• about 5-fold more CD8+ T cells are found in the spleen of double knockouts compared to mice homozygote for just the B2mtm1Jae allele
• numbers of CD8+ T cells in both the thymus and spleen are still well below normal numbers indicating the vast majority of CD8+ T cells are Class I-dependent
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hematopoietic system
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• about twice as much CD8+ T cells are found in the thymus of double knockouts compared to mice homozygote for just the B2mtm1Jae allele
• about 5-fold more CD8+ T cells are found in the spleen of double knockouts compared to mice homozygote for just the B2mtm1Jae allele
• numbers of CD8+ T cells in both the thymus and spleen are still well below normal numbers indicating the vast majority of CD8+ T cells are Class I-dependent
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immune system
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• mice display delayed development and attenuation of inflammatory bowel disease
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digestive/alimentary system
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• mice display delayed development and attenuation of inflammatory bowel disease
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Analysis Tools