Phenotypes associated with this allele

• Allele Symbol: Chek1^tm1Sje
• Allele Name: targeted mutation 1, Stephen J Elledge
• Allele ID: MGI:1926528
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Chek1^tm1Sje/Chek1^tm1Sje	Not Specified	MGI:2175781
ht2	Chek1^tm1Sje/Chek1^+	Not Specified	MGI:3588946
cx3	Chek1^tm1Sje/Chek1^tm1Sje Trp53^tm1Brd/Trp53^tm1Brd	involves: 129S7/SvEvBrd	MGI:3588912
cx4	Chek1^tm1Sje/Chek1^+ Tg(Wnt1)1Hev/0	Not Specified	MGI:3588947

Genotype
MGI:2175781

hm1

• Allelic Composition: Chek1^tm1Sje/Chek1^tm1Sje
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:62919 )

• die by E2.5-3.5

cellular

abnormal cell cycle checkpoint function ( J:62919 )

• initiation of mitosis occurred in mutant embryos despite exposure to DNA damage agents that normally arrest the cell cycle

increased embryonic tissue cell apoptosis ( J:62919 )

• massive DNA fragmentation during embryo degeneration

• TUNEL-positive cells are observed in mutant blastocysts, but not in control blastocysts

• ES cells made homozygous null appear apoptotic in culture

decreased cell proliferation ( J:62919 )

• mutant blastocysts stop proliferation and degenerate inside the zona pellucida

• failure of generation of homozygous ES cell colonies suggests that this gene product is required for cell proliferation

embryo

increased embryonic tissue cell apoptosis ( J:62919 )

• massive DNA fragmentation during embryo degeneration

• TUNEL-positive cells are observed in mutant blastocysts, but not in control blastocysts

• ES cells made homozygous null appear apoptotic in culture

failure of blastocyst to hatch from the zona pellucida ( J:62919 )

• a few cultured embryos do hatch, but do not contain an inner cell mass

Genotype
MGI:3588946

ht2

• Allelic Composition: Chek1^tm1Sje/Chek1⁺
• Genetic Background: Not Specified

neoplasm

neoplasm ( J:62919 )

N • no predisposition to early tumorigenesis in mice up to 18 months of age

Genotype
MGI:3588912

cx3

• Allelic Composition: Chek1^tm1Sje/Chek1^tm1Sje; Trp53^tm1Brd/Trp53^tm1Brd
• Genetic Background: involves: 129S7/SvEvBrd

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:62919 )

• die by E2.5-3.5

• the presence of the p53 mutant allele does not rescue or delay the lethality phenotype of the single Chek1^tm1Sje mutation

cellular

increased apoptosis ( J:62919 )

• TUNEL-positive cells are observed in double mutant blastocysts to a similar degree as the singly Chek1^tm1Sje mutant blastocysts, but not in control blastocysts

Genotype
MGI:3588947

cx4

• Allelic Composition: Chek1^tm1Sje/Chek1⁺; Tg(Wnt1)1Hev/0
• Genetic Background: Not Specified

neoplasm

increased mammary adenocarcinoma incidence ( J:62919 )

• earlier onset of mammary tumors (168 days versus 219 days of age) than in mice carrying only the transgene

• no differences in gross pathology, histopathology, or growth rates of these tumors compared to those in mice carrying only the transgene and not the Chek1 mutation

integument

increased mammary adenocarcinoma incidence ( J:62919 )

• earlier onset of mammary tumors (168 days versus 219 days of age) than in mice carrying only the transgene

• no differences in gross pathology, histopathology, or growth rates of these tumors compared to those in mice carrying only the transgene and not the Chek1 mutation

endocrine/exocrine glands

increased mammary adenocarcinoma incidence ( J:62919 )

• earlier onset of mammary tumors (168 days versus 219 days of age) than in mice carrying only the transgene

• no differences in gross pathology, histopathology, or growth rates of these tumors compared to those in mice carrying only the transgene and not the Chek1 mutation