Mouse Genome Informatics
hm1
    Il2rgtm1Cgn/Il2rgtm1Cgn
involves: 129 * CB20
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• 22-fold reduction in cellularity (J:22521)
• thymocytes do not activate in response to Con A (J:22521)
• 12-fold reduction in the spleen in 3-4 week old mice (J:22521)
• further loss occurs with age (J:22521)
• plasma cells in the gut are exceptionally rare (J:22521)
• 20-fold decrease in bone marrow though some cells are still present (J:22521)
• NK cells are not detected in the spleen (J:22521)
• 3 fold reduction of mature alphabeta T cells in the spleen (J:22521)
• reduction of T cells in lamina propia of gut is observed (J:22521)
• in vitro class switch using LPS and IL-4 fail to generate more IgG1-positive cells (J:22521)
• splenic T cells do not proliferate in response to Con A (J:22521)
• 12-fold reduction in number of T cells found in gut epithelium (J:22521)
• 8-fold reduction in cellularity (J:22521)
• no germinal centers are observed (J:22521)
• clusters of white pulp are diminished in number and size (J:22521)
• no IgE detected in the sera (J:22521)
• 3-fold reduction in sera levels (J:22521)
• no splenic NK killing activity is observed against a NK-sensitive target cell line (J:22521)
• Peyer's patchers are not observed (J:22521)

hematopoietic system
• 22-fold reduction in cellularity (J:22521)
• thymocytes do not activate in response to Con A (J:22521)
• 12-fold reduction in the spleen in 3-4 week old mice (J:22521)
• further loss occurs with age (J:22521)
• plasma cells in the gut are exceptionally rare (J:22521)
• 20-fold decrease in bone marrow though some cells are still present (J:22521)
• NK cells are not detected in the spleen (J:22521)
• 3 fold reduction of mature alphabeta T cells in the spleen (J:22521)
• reduction of T cells in lamina propia of gut is observed (J:22521)
• in vitro class switch using LPS and IL-4 fail to generate more IgG1-positive cells (J:22521)
• splenic T cells do not proliferate in response to Con A (J:22521)
• 12-fold reduction in number of T cells found in gut epithelium (J:22521)
• 8-fold reduction in cellularity (J:22521)
• no germinal centers are observed (J:22521)
• clusters of white pulp are diminished in number and size (J:22521)
• no IgE detected in the sera (J:22521)
• 3-fold reduction in sera levels (J:22521)
• no splenic NK killing activity is observed against a NK-sensitive target cell line (J:22521)

endocrine/exocrine glands
• 22-fold reduction in cellularity (J:22521)
• thymocytes do not activate in response to Con A (J:22521)


Mouse Genome Informatics
cx2
    Il2rgtm1Cgn/Y
Rag2tm1Fwa/Rag2tm1Fwa

B6.129-Rag2tm1Fwa Il2rgtm1Cgn
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• mice lack all B cells (J:132257)
• mice lack all NK cells (J:132257)
• NK cells that are transferred into these mice are able to proliferate with a lifespan of about 10 days (J:132257)
• mice lack all T cells (J:132257)

hematopoietic system
• mice lack all B cells (J:132257)
• mice lack all NK cells (J:132257)
• NK cells that are transferred into these mice are able to proliferate with a lifespan of about 10 days (J:132257)
• mice lack all T cells (J:132257)


Mouse Genome Informatics
cx3
    Il2rgtm1Cgn/Il2rgtm1Cgn
Rag2tm1Fwa/Rag2tm1Fwa

involves: 129P2/OlaHsd * 129S/SvEv
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• Ncr(NKp46)+ NK1.1+ NK cells are essentially absent (J:142654)
• the NKp46+CD127+ NK1.1- subset of intestinal natural killer cells is also absent (J:142654)
• there is no interleukin 22 (IL-22) secretion by colonic lamina propia cells isolated from mice infected with C. rodentium compared to controls that secrete detectable amounts of IL-22 (J:142654)
• mice are extremely susceptible to C. rodentium infection, with all mice showing dramatic weight loss and succumbing to infection by day 7 (J:142654)
• controls (Rag2 knockouts, Rag2 and Il2rg double knockouts) are not susceptible to infection (J:142654)

hematopoietic system
• Ncr(NKp46)+ NK1.1+ NK cells are essentially absent (J:142654)
• the NKp46+CD127+ NK1.1- subset of intestinal natural killer cells is also absent (J:142654)


Mouse Genome Informatics
cx4
    Rag2tm1Fwa/Rag2tm1Fwa
Cd47tm1Fpl/Cd47tm1Fpl
Il2rgtm1Cgn/Y

involves: 129P2/OlaHsd * 129S/SvEv * 129S7/SvEvBrd * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells
• a high proportion of CD38+ CD8+ T cells appear activated in HIV-infected BLT transplanted mice
• spleen and lymph nodes lack lymphocytes
• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells
• mice fail to develop peripheral lymph nodes
• 54% of BLT transplanted mice inoculated intrarectally with HIV become infected with plasma viral titers peaking between 2 and 4 weeks
• 100% of transplanted mice inoculated intraperitoneally with HIV become infected
• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells
• a high proportion of CD8+ T cells appear activated in HIV-infected BLT transplanted mice
• infected mice produce HIV-specific antibodies and can mount HIV-specific T cell responses
• mice are tolerized to xenotransplantation with human bone marrow, liver, thymus (BLT)
• mice average 1x106 human leukocytes/mL blood 12 weeks after transplantation and levels remain stable or improve through 21 weeks
• >80% of leukocytes in peripheral circulation are human
• transplanted mice have higher levels of multipotent bone marrow/hematopoietic stem cells than mice heterozygous for the Cd47tm1Fpl allele
• GVHD is not evident in mice 25 weeks post-transplantation
• human thymic organoids are highly vascularized
• large and small intestines of transplanted mice contain CD4+ and CD8+ human T cells

hematopoietic system
• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells
• a high proportion of CD38+ CD8+ T cells appear activated in HIV-infected BLT transplanted mice
• spleen and lymph nodes lack lymphocytes
• HIV-infected BLT transplanted mice exhibit a loss of CD4+ T cells and an increase in CD8+ T cells


Mouse Genome Informatics
cx5
    Il2rgtm1Cgn/Il2rgtm1Cgn
Kittm1.1Bsm/Kittm1.1Bsm

involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
hematopoietic system
• similar to in Il2rgtm1Cgn homozygotes, T cell development is blocked at the TN3 to TN4 stage (J:90485)

immune system
• similar to in Il2rgtm1Cgn homozygotes, T cell development is blocked at the TN3 to TN4 stage (J:90485)


Mouse Genome Informatics
ot6
    Il2rgtm1Cgn/Y
involves: 129 * CB20
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• 22-fold reduction in cellularity (J:22521)
• 10-fold less cellularity of thymus from fetal day E15.5 through birth (J:111821)
• 20-fold less cellularity by six days of age (J:111821)
• thymocytes do not activate in response to Con A (J:22521)
• 12-fold reduction in the spleen in 3-4 week old mice (J:22521)
• further loss occurs with age (J:22521)
• plasma cells in the gut are exceptionally rare (J:22521)
• 20-fold decrease in bone marrow though some cells are still present (J:22521)
• NK cells are not detected in the spleen (J:22521)
• 3 fold reduction of mature alphabeta T cells in the spleen (J:22521)
• reduction of T cells in lamina propia of gut is observed (J:22521)
• no gamma-delta T cells are found in peripheral lymphnoid organs or skin (J:111821)
• in vitro class switch using LPS and IL-4 fail to generate more IgG1-positive cells (J:22521)
• splenic T cells do not proliferate in response to Con A (J:22521)
• complete absence of gamma-delta T cells in adult thymus (J:111821)
• few gamma-delta T cells found in fetal thymus (J:111821)
• TCR-gamma rearrangements are severely reduced (J:111821)
• 12-fold reduction in number of T cells found in gut epithelium (J:22521)
• 8-fold reduction in cellularity (J:22521)
• no germinal centers are observed (J:22521)
• clusters of white pulp are diminished in number and size (J:22521)
• no IgE detected in the sera (J:22521)
• 3-fold reduction in sera levels (J:22521)
• no splenic NK killing activity is observed against a NK-sensitive target cell line (J:22521)
• Peyer's patchers are not observed (J:22521)

hematopoietic system
• 22-fold reduction in cellularity (J:22521)
• 10-fold less cellularity of thymus from fetal day E15.5 through birth (J:111821)
• 20-fold less cellularity by six days of age (J:111821)
• thymocytes do not activate in response to Con A (J:22521)
• 12-fold reduction in the spleen in 3-4 week old mice (J:22521)
• further loss occurs with age (J:22521)
• plasma cells in the gut are exceptionally rare (J:22521)
• 20-fold decrease in bone marrow though some cells are still present (J:22521)
• NK cells are not detected in the spleen (J:22521)
• 3 fold reduction of mature alphabeta T cells in the spleen (J:22521)
• reduction of T cells in lamina propia of gut is observed (J:22521)
• no gamma-delta T cells are found in peripheral lymphnoid organs or skin (J:111821)
• in vitro class switch using LPS and IL-4 fail to generate more IgG1-positive cells (J:22521)
• splenic T cells do not proliferate in response to Con A (J:22521)
• complete absence of gamma-delta T cells in adult thymus (J:111821)
• few gamma-delta T cells found in fetal thymus (J:111821)
• TCR-gamma rearrangements are severely reduced (J:111821)
• 12-fold reduction in number of T cells found in gut epithelium (J:22521)
• 8-fold reduction in cellularity (J:22521)
• no germinal centers are observed (J:22521)
• clusters of white pulp are diminished in number and size (J:22521)
• no IgE detected in the sera (J:22521)
• 3-fold reduction in sera levels (J:22521)
• no splenic NK killing activity is observed against a NK-sensitive target cell line (J:22521)

endocrine/exocrine glands
• 22-fold reduction in cellularity (J:22521)
• 10-fold less cellularity of thymus from fetal day E15.5 through birth (J:111821)
• 20-fold less cellularity by six days of age (J:111821)
• thymocytes do not activate in response to Con A (J:22521)