Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Cgn mutation
(3 available);
any
Igh-J mutation
(13 available)
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immune system
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• B cell development is blocked at the pre-B cell stage
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• no mature B cells are formed
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hematopoietic system
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• B cell development is blocked at the pre-B cell stage
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• no mature B cells are formed
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Allelic
Composition |
Igh-Jtm1Cgn/Igh-J+
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Genetic
Background |
involves: 129P2/OlaHsd * C57BL/6 |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Cgn mutation
(3 available);
any
Igh-J mutation
(13 available)
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immune system
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• all B cells in the peripheral blood express only surface IgMb while wild-type show equivalent numbers of IgMa and IgMb-bearing cells
• cultured splenic B cells stimulated with LPS and Il-4 show defective switch recombination at Smu in cis (on mutant chromosome) compared to wild-type B cells
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hematopoietic system
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• all B cells in the peripheral blood express only surface IgMb while wild-type show equivalent numbers of IgMa and IgMb-bearing cells
• cultured splenic B cells stimulated with LPS and Il-4 show defective switch recombination at Smu in cis (on mutant chromosome) compared to wild-type B cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Cgn mutation
(3 available);
any
Igh-J mutation
(13 available)
Ightm4Cgn mutation
(0 available);
any
Igh mutation
(43 available)
Tg(Cr2-cre)3Cgn mutation
(2 available)
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immune system
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• mice lack mature BCR positive B cells
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hematopoietic system
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• mice lack mature BCR positive B cells
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immune system
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• mice exhibit an accumulation of IgM negative B cells compared to in Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
• mice exhibit an efficient rescue of BCR negative B cells compared with Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
• however, follicle B cells are rescued compared to in Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
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hematopoietic system
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• mice exhibit an accumulation of IgM negative B cells compared to in Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
• mice exhibit an efficient rescue of BCR negative B cells compared with Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
• however, follicle B cells are rescued compared to in Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Foxo1tm1.1Rdp mutation
(0 available);
any
Foxo1 mutation
(31 available)
Igh-Jtm1Cgn mutation
(3 available);
any
Igh-J mutation
(13 available)
Ightm4Cgn mutation
(0 available);
any
Igh mutation
(43 available)
Tg(Cr2-cre)3Cgn mutation
(2 available)
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immune system
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• mice exhibit a limited rescue of IgM negative B cells compared to in Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
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hematopoietic system
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• mice exhibit a limited rescue of IgM negative B cells compared to in Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
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immune system
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• mice lack mature BCR positive B cells
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hematopoietic system
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• mice lack mature BCR positive B cells
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immune system
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• mice exhibit a very limited rescue of BCR negative B cells in several lymphoid organs compared with Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
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hematopoietic system
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• mice exhibit a very limited rescue of BCR negative B cells in several lymphoid organs compared with Igh-Jtm1Cgn/Ightm4Cgn Tg(Cr2-cre)3Cgn mice
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immune system
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• mice lack mature BCR positive B cells
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hematopoietic system
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• mice lack mature BCR positive B cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Cgn mutation
(3 available);
any
Igh-J mutation
(13 available)
Ightm4Cgn mutation
(0 available);
any
Igh mutation
(43 available)
Tg(Cr2-cre)3Cgn mutation
(2 available)
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hematopoietic system
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• B cell numbers are reduced 50% in the bone marrow, by about 2/3 in follicular and marginal zones of the spleen, and strongly reduced in the inguinal lymph nodes
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immune system
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• B cell numbers are reduced 50% in the bone marrow, by about 2/3 in follicular and marginal zones of the spleen, and strongly reduced in the inguinal lymph nodes
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Cgn mutation
(3 available);
any
Igh-J mutation
(13 available)
Ightm11.1Cgn mutation
(0 available);
any
Igh mutation
(43 available)
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immune system
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• frequency of joints in mature B cells that have undergone by VH replacement that contain the extra arginine that would be generated at the junction as well as the arginine encoded at the 3' end of the D23stop allele is only 12%, indicating that counterselection of complementarity region 3s rich in positively charged amino acids occurs
• VH replacement and direct VH to DH joining compete inefficiently with canonical VDJ recombination in B cell development in vivo in 2.5 month old mice
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hematopoietic system
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• frequency of joints in mature B cells that have undergone by VH replacement that contain the extra arginine that would be generated at the junction as well as the arginine encoded at the 3' end of the D23stop allele is only 12%, indicating that counterselection of complementarity region 3s rich in positively charged amino acids occurs
• VH replacement and direct VH to DH joining compete inefficiently with canonical VDJ recombination in B cell development in vivo in 2.5 month old mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igh-Jtm1Cgn mutation
(3 available);
any
Igh-J mutation
(13 available)
Ightm1Leck mutation
(0 available);
any
Igh mutation
(43 available)
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immune system
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• the percentage of B cells expressing lambda light chain is about 4 times higher than in wild-type mice
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hematopoietic system
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• the percentage of B cells expressing lambda light chain is about 4 times higher than in wild-type mice
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Analysis Tools