Phenotypes associated with this allele

• Allele Symbol: H2-DMa^tm1Luc
• Allele Name: targeted mutation 1, Luc van Kaer
• Allele ID: MGI:1861933
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	H2-DMa^tm1Luc/H2-DMa^tm1Luc	B6.129S4-H2-Dma^tm1Luc	MGI:3851338
hm2	H2-DMa^tm1Luc/H2-DMa^tm1Luc	involves: 129S4/SvJae	MGI:2176507
hm3	H2-DMa^tm1Luc/H2-DMa^tm1Luc	involves: 129S4/SvJae * C57BL/6	MGI:3688898
cx4	H2-DMa^tm1Luc/H2-DMa^tm1Luc Cd74^tm1Doi/Cd74^tm1Doi	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:2665538
cx5	Cd74^tm1Liz/Cd74^tm1Liz H2-DMa^tm1Luc/H2-DMa^tm1Luc Tg(CD74)AR194Ayr/?	involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J	MGI:3760430
cx6	Cd74^tm1Liz/Cd74^tm1Liz H2-DMa^tm1Luc/H2-DMa^tm1Luc Tg(CD74*)1Ayr/0	involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J	MGI:3839583

Genotype
MGI:3851338

hm1

• Allelic Composition: H2-DMa^tm1Luc/H2-DMa^tm1Luc
• Genetic Background: B6.129S4-H2-Dma^tm1Luc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T-helper 1 physiology ( J:125654 )

• in vivo, Th1 response to MOG or PLP is reduced compared to in wild-type mice

decreased susceptibility to type IV hypersensitivity reaction ( J:125654 )

• slightly compared to in wild-type mice when wild-type MOG T cells are transferred

abnormal antigen presentation ( J:125654 )

• antigen presenting cells cannot process and present recombinant MOG protein unlike wild-type cells

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:125654 )

• whether MOG-primed or adoptively transferred with MOG-specific T blasts, mice fail to develop symptoms of experimental autoimmune encephalomyelitis (EAE) unlike wild-type mice

• PLP-treated mice fail to develop EAE unlike similarly treated wild-type mice

• however, MOG-primed are capable of inducing encephalomyelitis in a wild-type host

hematopoietic system

abnormal T-helper 1 physiology ( J:125654 )

• in vivo, Th1 response to MOG or PLP is reduced compared to in wild-type mice

Genotype
MGI:2176507

hm2

• Allelic Composition: H2-DMa^tm1Luc/H2-DMa^tm1Luc
• Genetic Background: involves: 129S4/SvJae

mortality/aging

premature death ( J:31625 )

• mutants occasionally die without evidence of severe infection

growth/size/body

decreased body size ( J:31625 )

• smaller than control littermates

immune system

abnormal positive T cell selection ( J:125548 )

• CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow

decreased CD4-positive, alpha-beta T cell number ( J:31625 , J:45185 )

• reduced numbers of CD4+CD8- T cells (J:31625)

• decreased number of peripheral CD4+ T cells (J:45185)

abnormal CD4-positive, alpha-beta T cell physiology ( J:31625 )

• CD4+CD8- T cells exhibit altered reactivity to antigen-presenting cells from a variety of mouse strains

abnormal antigen presentation via MHC class II ( J:31625 )

• inability to present intact protein antigen to class II-restricted T cells

• reduced ability to present exogenous peptides

abnormal level of surface class II molecules ( J:45185 )

• abnormal level of surface class II molecules on B cells

decreased interferon-gamma secretion ( J:45185 )

• decreased interferon gamma production

increased susceptibility to bacterial infection ( J:45185 )

• increased susceptibility to Leishmania major infection

• by 17 weeks, mice have developed large (>4mm) non-healing footpad lesions, whereas controls do not show lesions larger than 4mm

hematopoietic system

abnormal positive T cell selection ( J:125548 )

• CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow

decreased CD4-positive, alpha-beta T cell number ( J:31625 , J:45185 )

• reduced numbers of CD4+CD8- T cells (J:31625)

• decreased number of peripheral CD4+ T cells (J:45185)

abnormal CD4-positive, alpha-beta T cell physiology ( J:31625 )

• CD4+CD8- T cells exhibit altered reactivity to antigen-presenting cells from a variety of mouse strains

Genotype
MGI:3688898

hm3

• Allelic Composition: H2-DMa^tm1Luc/H2-DMa^tm1Luc
• Genetic Background: involves: 129S4/SvJae * C57BL/6

immune system

abnormal antigen presentation ( J:72243 )

• splenic antigen presenting cells (APCs) can present an encephalitogenic MOG peptide P35-55 to I-A^b-restricted p35-55-specific T cells but a one log-higher concentration of peptide is needed to stimulate proliferation, compared to wild-type or Cd74 deficient cells; mutant APCs are ineffective at presenting intact MOG to T cells compared with wild-type APCs, suggesting that processing is required for presentation of intact MOG

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:72243 )

• mice do not develop experimental autoimmune encephalitis (EAE) upon direct immunization with p35-55 while wild-type mice do develop EAE

• adoptive transfer of wild-type encephalitogenic T cells does not induce EAE in mutant recipients

Genotype
MGI:2665538

cx4

• Allelic Composition: H2-DMa^tm1Luc/H2-DMa^tm1Luc; Cd74^tm1Doi/Cd74^tm1Doi
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6

immune system

abnormal level of surface class II molecules ( J:45185 )

• reduced level of surface class II molecules on B cells

increased susceptibility to parasitic infection ( J:45185 )

• increased susceptibility to Leishmania major infection

Genotype
MGI:3760430

cx5

• Allelic Composition: Cd74^tm1Liz/Cd74^tm1Liz; H2-DMa^tm1Luc/H2-DMa^tm1Luc; Tg(CD74)AR194Ayr/?
• Genetic Background: involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J

immune system

decreased CD4-positive, alpha-beta T cell number ( J:88717 )

• the number of CD4+ T cells is reduced to levels below wild-type but similar to those observed in Cd74^tm1Liz H2-DMa^tm1Luc homozygotes

abnormal antigen presentation via MHC class II ( J:88717 )

• unlike in wild-type cells, splenocytes are unable to elicit presentation of MHC class II exogenous antigens or of two endogenous antigens (IgM and beta-2 microglobulin)

• however, Tg(CD74)AR194Ayr completely rescues the MHC class II peptide loading observed in Cd74^tm1Liz H2-DMa^tm1Luc homozygotes and presentation of endogenous antigens for CD22 is normal

• after incubation with Ealpha peptide, loading of MHC class II is less than on similarly treated Cd74^tm1Liz H2-DMa^tm1Luc Tg(CD74*)AR194Ayr splenocytes

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:88717 )

• the number of CD4+ T cells is reduced to levels below wild-type but similar to those observed in Cd74^tm1Liz H2-DMa^tm1Luc homozygotes

Genotype
MGI:3839583

cx6

• Allelic Composition: Cd74^tm1Liz/Cd74^tm1Liz; H2-DMa^tm1Luc/H2-DMa^tm1Luc; Tg(CD74*)1Ayr/0
• Genetic Background: involves: 129S/SvEv * 129S4/SvJae * C57BL/6J * DBA/2J

immune system

decreased CD4-positive, alpha-beta T cell number ( J:88717 )

• the number of CD4+ T cells is rescued to 60% of normal

abnormal antigen presentation via MHC class II ( J:88717 )

• Tg(CD74)AR194Ayr partially rescues the MHC class II peptide loading observed in Cd74^tm1Liz H2-DMa^tm1Luc homozygotes

• after incubation with Ealpha peptide, loading of MHC class II is greater than on similarly treated Cd74^tm1Liz H2-DMa^tm1Luc Tg(CD74)AR194Ayr splenocytes

• splenocytes elicit greater antigen presentations than in Cd74^tm1Liz H2-DMa^tm1Luc Tg(CD74)AR194Ayr splenocytes

• splenocytes elicit presentation of exogenous protein antigens derived from one but not two antigen

• splenocyte presentaton of IgM and beta-2 microglobulin epitpes is less efficient than in Cd74^tm1Liz Tg(CD74)AR194Ayr or Cd74^tm1Liz Tg(CD74*)AR194Ayr mice

• however, splenocytes can elicit presentation from two endogenous protein antigens (IgM and beta-2 microglobulin)

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:88717 )

• the number of CD4+ T cells is rescued to 60% of normal