Mouse Genome Informatics
hm1
    Tnftm1Jods/Tnftm1Jods
C57BL/6-Ltatm1Jods
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• B cell follicles are replaced by a rim-like structure and the T and B cell areas are less distinct
• onset of experimental autoimmune encephalomyelitis (EAE) is delayed by 6 days and the overall duration is reduced compared to in wild-type mice
• treatment of mice with TNFR-IgG does not alter disease course as in wild-type mice
• however, the progress of EAE parallels that in wild-type mice

hematopoietic system
• B cell follicles are replaced by a rim-like structure and the T and B cell areas are less distinct


Mouse Genome Informatics
hm2
    Tnftm1Jods/Tnftm1Jods
C57BL/6-Tnftm1Jods
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• cytokine expression levels are elevated 2-4 days before onset of clinical signs of disease in wild-type, but levels are elevated only at onset of disease in mutants
• immunization with pertussis toxin results in clinical EAE appearing at day 12 and peaking at ~day 18, while in mutants, clinical manifestation of disease is delayed ~5 days, peaking at ~23 days; duration of disease is reduced in mutants compared to wild type

homeostasis/metabolism
• cytokine expression levels are elevated 2-4 days before onset of clinical signs of disease in wild-type, but levels are elevated only at onset of disease in mutants


Mouse Genome Informatics
hm3
    Tnftm1Jods/Tnftm1Jods
involves: C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
immune system
• B and T cell zones not well demarcated (J:43602)
• loss of B and T cell area segregation (J:98591)
• the MOMA-1+ marginal zone metallophilic macrophages are absent
• absence of primary B cell follicles (J:43602)
• B cells form a continuous ring around PALS (J:43602)
• absent primary B cell follicles, with B cells forming a ring-like structure around the T cell area (J:98591)
• no formation of PNA+ germinal centers upon immunization
• reduced number of follicular dendritic cells in B cell zones of the spleen
• loss of defined B and T cell structures, with intermixing of cell types (J:43602)
• reduced number of B cells (J:43602)
• exhibit a flattened appearance and T and B cells intermix freely (J:98591)
• single, enlarged follicle
• loss of primary B cell follicles
• delay in the onset of clinical signs in an experimental autoimmune encephalomyelitis (EAE) model, however, disease progressed with similar severity and incidence as in wild-type once it was established (J:98591)
• development of EAE under suboptimal immunization conditions reduced the incidence of disease below 40%, with mild clinical signs that resolved quickly unlike in wild-type (J:98591)
• resistant to endotoxic shock induced by both high and low doses of LPS
• homozygous null mice were unable to control M. tuberculosis growth, showed massive inflammation with extensive necrosis in the lung, developed pneumonia and had a mean survival time of 33 days (J:98170)
• S. aureus-infected mice exhibit greater weight loss and decreased bacterial clearance compared with similarly treated wild-type mice (J:115034)

hematopoietic system
• B and T cell zones not well demarcated (J:43602)
• loss of B and T cell area segregation (J:98591)
• the MOMA-1+ marginal zone metallophilic macrophages are absent
• absence of primary B cell follicles (J:43602)
• B cells form a continuous ring around PALS (J:43602)
• absent primary B cell follicles, with B cells forming a ring-like structure around the T cell area (J:98591)
• no formation of PNA+ germinal centers upon immunization