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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Adrb2tm1Bkk
targeted mutation 1, Brian K Kobilka
MGI:1859675
Summary 7 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Adrb2tm1Bkk/Adrb2tm1Bkk FVB.129-Adrb2tm1Bkk MGI:3710655
hm2
Adrb2tm1Bkk/Adrb2tm1Bkk involves: 129S1/Sv * 129X1/SvJ MGI:3579020
hm3
Adrb2tm1Bkk/Adrb2tm1Bkk involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2 * FVB/N MGI:5565473
ht4
Adrb2tm1Bkk/Adrb2+ involves: 129S1/Sv * 129X1/SvJ MGI:3579023
cx5
Adrb1tm1Bkk/Adrb1tm1Bkk
Adrb2tm1Bkk/Adrb2tm1Bkk
involves: 129S1/Sv * 129X1/SvJ MGI:3579029
cx6
Adrb1tm1Bkk/Adrb1tm1Bkk
Adrb2tm1Bkk/Adrb2tm1Bkk
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2 * FVB/N MGI:2388098
cx7
Adrb1tm1Bkk/Adrb1tm1Bkk
Adrb2tm1Bkk/Adrb2tm1Bkk
Adrb3tm1Lowl/Adrb3tm1Lowl
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2 * FVB/N MGI:2388265


Genotype
MGI:3710655
hm1
Allelic
Composition
Adrb2tm1Bkk/Adrb2tm1Bkk
Genetic
Background
FVB.129-Adrb2tm1Bkk
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adrb2tm1Bkk mutation (1 available); any Adrb2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
adipose tissue
• represent a smaller proportion of total body weight than wild-type fat pads

behavior/neurological
N
• daily locomotor activity is similar to wild-type during 48 hours of observation

cardiovascular system
• mutants show trend to increased heart rate with epinephrine, whereas wild-type show a trend to decreased rate, but difference is not significant
• isoproterenol treatment induced tachycardia in mutants and wild-type similarly
• in response to isoproterenol treatement, the normal hypotensive response was blunted relative to rapid response in wild-type
• daily locomotor activity is similar to wild-type during 48 hours of observation

growth/size/body
• weight is significantly less than wild-type mice

homeostasis/metabolism
N
• no significant differences are seen in body density, free fatty acid levels, and serum glycerol relative to controls
• ratio is lower in mutants during exercise (greater oxygen consumption than wild-type)
• on a graded treadmill, null mice show longer period of exercise compared to wild-type; null mice cover 582 meters vs 471 meters for wild-type




Genotype
MGI:3579020
hm2
Allelic
Composition
Adrb2tm1Bkk/Adrb2tm1Bkk
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adrb2tm1Bkk mutation (1 available); any Adrb2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
skeleton
• decreased osteoclast surface in homozygous mutant bones
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation
• bone resorption did not increase in gonadectomized homozygous mutant mice
• decrease in urinary elimination of deoxypyridinoline, a marker of osteoclast function
• increased bone volume/tissue volume and bone formation rate, showing a severe high bone mass phenotype at 6 months of age
• long-term leptin intracerebroventricular infusion or gonadectomy did not reduce bone mass of homozygous mutant mice
• increased bone formation rate and decreased bone resorption
• isoproterenol did not enhance the generation of osteoclasts when homozygous mutant osteoblasts were co-cultured with wildtype bone marrow macrophages, like it did in wildtype

hematopoietic system
• decreased osteoclast surface in homozygous mutant bones
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation
• bone resorption did not increase in gonadectomized homozygous mutant mice
• decrease in urinary elimination of deoxypyridinoline, a marker of osteoclast function

immune system
• decreased osteoclast surface in homozygous mutant bones
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation
• bone resorption did not increase in gonadectomized homozygous mutant mice
• decrease in urinary elimination of deoxypyridinoline, a marker of osteoclast function

cellular
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation




Genotype
MGI:5565473
hm3
Allelic
Composition
Adrb2tm1Bkk/Adrb2tm1Bkk
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adrb2tm1Bkk mutation (1 available); any Adrb2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• increase in apoptosis in the ganglion cell layer
• decrease in retinal thickness at 2 months of age
• GFAP protein levels are increased in retinal Muller cells, indicating activated Muller cells
• increase in apoptosis in the ganglion cell layer
• decrease in cell number in the ganglion cell layer at 2 months of age
• oscillatory potential amplitudes are reduced at 2 months of age
• A-wave amplitudes are reduced at high light intensity
• B-wave amplitudes are reduced at 2 months of age

immune system
• increase in TNF-alpha in retinal lysates

nervous system
• GFAP protein levels are increased in retinal Muller cells, indicating activated Muller cells
• decrease in cell number in the ganglion cell layer at 2 months of age

cellular
• increase in apoptosis in the ganglion cell layer




Genotype
MGI:3579023
ht4
Allelic
Composition
Adrb2tm1Bkk/Adrb2+
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adrb2tm1Bkk mutation (1 available); any Adrb2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
skeleton
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation
• increased bone volume/tissue volume and bone formation rate, showing a severe high bone mass phenotype at 6 months of age
• increased bone formation rate and decreased bone resorption

hematopoietic system
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation

immune system
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation

cellular
• decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts, indicating defective osteoclast differentiation




Genotype
MGI:3579029
cx5
Allelic
Composition
Adrb1tm1Bkk/Adrb1tm1Bkk
Adrb2tm1Bkk/Adrb2tm1Bkk
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adrb1tm1Bkk mutation (1 available); any Adrb1 mutation (4 available)
Adrb2tm1Bkk mutation (1 available); any Adrb2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
skeleton
N
• exhibit normal bone mass at 6 months of age




Genotype
MGI:2388098
cx6
Allelic
Composition
Adrb1tm1Bkk/Adrb1tm1Bkk
Adrb2tm1Bkk/Adrb2tm1Bkk
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adrb1tm1Bkk mutation (1 available); any Adrb1 mutation (4 available)
Adrb2tm1Bkk mutation (1 available); any Adrb2 mutation (13 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• abnormal responses to pharmacological stimuli; attenuation of both the normal tachycardia and hypotensive responses to isoproterenol administration and bradycardia and monophasic hypertension in response to epinephrine administration
• reduced cardiac contractility in awake and in anesthetized mice
• decreased heart rate in response to exercise, but normal basal heart rate, normal basal mean arterial blood pressure and normal blood pressure changes in response to exercise

homeostasis/metabolism
• reduced O2 consumption during exercise and reduced CO2 production during exercise, but normal total exercise capacity
• abnormal responses to pharmacological stimuli; attenuation of the normal bradycardia and monophasic hypertension in response to epinephrine administration
• abnormal responses to pharmacological stimuli; attenuation of the normal tachycardia and hypotensive responses to isoproterenol administration

muscle
• reduced cardiac contractility in awake and in anesthetized mice




Genotype
MGI:2388265
cx7
Allelic
Composition
Adrb1tm1Bkk/Adrb1tm1Bkk
Adrb2tm1Bkk/Adrb2tm1Bkk
Adrb3tm1Lowl/Adrb3tm1Lowl
Genetic
Background
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Adrb1tm1Bkk mutation (1 available); any Adrb1 mutation (4 available)
Adrb2tm1Bkk mutation (1 available); any Adrb2 mutation (13 available)
Adrb3tm1Lowl mutation (1 available); any Adrb3 mutation (17 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
adipose tissue
• obesity attributable to increased fat mass
• interscapular brown adipose tissue (BAT) enlarged and pale
• BAT contained large cells with unilocular triglyceride deposits

endocrine/exocrine glands
N
• normal thyroid hormone levels

growth/size/body
• on regular Chow diet, mildly obese by 20 weeks; increased obesity on high fat diet (58% of kcal from fat)
• normal food intake and normal physical activity on both normal and high fat diets were observed, however

homeostasis/metabolism
• normal body temperature under conventional housing conditions, but exposure to cold (22oC) produced rapid decline in core body temperature
• failure of diet-induced thermogenesis
• increased leptin levels
• reduced metabolic rate, measured by O2 consumption
• isoproterenol exposure failed to increase O2 consumption

Mouse Models of Human Disease
OMIM ID Ref(s)
Obesity 601665 J:79309





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last database update
06/15/2016
MGI 6.04
The Jackson Laboratory