Mouse Genome Informatics
hm1
    Krastm1Mok/Krastm1Mok
involves: 129S/SvEv * C57BL/6J
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• homozygotes die between E12.5 and term

cardiovascular system
• at E15.5, 2 out of 6 homozygotes display extremely thin ventricular walls along with a swollen heart and a rounded apex, while 3 out of 6 mutants show thin ventricular walls in the absence of swelling; the remaining one homozygote displays a morphologically normal heart
• at E15.5, homozygotes display a thin ventricular myocardium relative to wild-type mice
• at E10.5-E13.5, mutant hearts appear to be smaller than wild-type hearts
• at E15.5, 5 out of 6 homozygotes exhibit thin ventricular walls, in the absence of ventricular septal defects
• no significant differences in ventricular wall thickness are observed at E11.5 and E13.5
• at E15.5, mutant hearts display insufficient cardiomyocyte proliferation, in the absence of abnormal cell differentiation or cell death

embryogenesis
• at E10.5-E13.5, homozygous embryos appear to be shorter than wild-type embryos

growth/size
• at E10.5-E13.5, homozygous embryos appear to be shorter than wild-type embryos

nervous system
• at E11.5 and E12.5, homozygotes exhibit a significantly increased apoptosis of motoneurons in the ventral horn of lower medulla and the upper cervical spinal cord

muscle
• at E15.5, mutant hearts display insufficient cardiomyocyte proliferation, in the absence of abnormal cell differentiation or cell death

hematopoietic system
N
• at E12.5, homozygotes exhibit neither anemia nor abnormal red blood cells in peripheral blood vessels (J:42943)

liver/biliary system
N
• at E12.5, homozygotes display a histologically normal liver relative to wild-type mice (J:42943)

cellular
• at E15.5, mutant hearts display insufficient cardiomyocyte proliferation, in the absence of abnormal cell differentiation or cell death
• at E11.5 and E12.5, homozygotes exhibit a significantly increased apoptosis of motoneurons in the ventral horn of lower medulla and the upper cervical spinal cord


Mouse Genome Informatics
ht2
    Krastm1Mok/Kras+
involves: 129S/SvEv * C57BL/6J
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
normal phenotype
• heterozygotes are viable, fertile and display no behavioral deficits or spontaneous tumor formation up to 20 months of age


Mouse Genome Informatics
cx3
    Krastm1Mok/Kras+
Nrastm1Mok/Nras+

B6.129S-Nrastm1Mok Krastm1Mok
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• 19 of 26 mice develop chylous ascites unlike wild-type mice


Mouse Genome Informatics
cx4
    Krastm1Mok/Kras+
Nrastm1Mok/Nrastm1Mok

either: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * C57BL/6 * DBA/2)
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• most deaths occur between E18.5 and birth
• do not survive to weaning


Mouse Genome Informatics
cx5
    Hrastm1Mok/Hrastm1Mok
Krastm1Mok/Krastm1Mok

either: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * C57BL/6 * DBA/2)
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• development is normal to E9.5
• most are dead by E11.5
• none are found at E13.5


Mouse Genome Informatics
cx6
    Krastm1Mok/Krastm1Mok
Tg(HRAS)7Mok/0

either: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * C57BL/6 * DBA/2)
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
normal phenotype
• mice survive and reproduce
• hearts are normal


Mouse Genome Informatics
cx7
    Hrastm1Mok/Hrastm1Mok
Krastm1Mok/Krastm1Mok
Nrastm1Mok/Nrastm1Mok
Tg(HRAS)7Mok/0

either: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * C57BL/6 * DBA/2)
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
normal phenotype
• born in expected numbers


Mouse Genome Informatics
cx8
    Flt4tm1.1Ichi/Flt4+
Krastm1Mok/Kras+
Tg(HRAS)2Jic/0

involves: 129S/SvEv * BALB/c * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• 1 of 13 mice develop chylous ascites unlike wild-type mice

immune system
• few mice exhibit lymphatic vessel hypoplasia


Mouse Genome Informatics
cx9
    Hrastm1Mok/Hrastm1Mok
Krastm1Mok/Kras+
Nrastm1Mok/Nras+
Tg(HRAS)2Jic/0

involves: 129S/SvEv * BALB/c * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
N
• no mice exhibit chylous ascites (J:159024)


Mouse Genome Informatics
cx10
    Krastm1Mok/Kras+
Nrastm1Mok/Nrastm1Mok
Tg(HRAS)2Jic/0

involves: 129S/SvEv * BALB/c * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
N
• no mice exhibit chylous ascites (J:159024)


Mouse Genome Informatics
cx11
    Flt4tm1.1Ichi/Flt4+
Krastm1Mok/Kras+

involves: 129S/SvEv * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• all mice develop chylous ascites unlike wild-type mice
• intestinal

immune system
• mice develop lymphatic vessel hypoplasia unlike wild-type mice


Mouse Genome Informatics
cx12
    Krastm1Mok/Kras+
Nrastm1Mok/Nras+

involves: 129S/SvEv * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• 5 of 76 mice develop chylous ascites unlike wild-type mice

immune system
• in the small intestine


Mouse Genome Informatics
cx13
    Hrastm1Mok/Hrastm1Mok
Krastm1Mok/Kras+

involves: 129S/SvEv * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• 20 of 135 mice develop chylous ascites unlike wild-type mice


Mouse Genome Informatics
cx14
    Hrastm1Mok/Hrastm1Mok
Krastm1Mok/Kras+
Nrastm1Mok/Nras+

involves: 129S/SvEv * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• 35 of 37 mice develop chylous ascites unlike wild-type mice


Mouse Genome Informatics
cx15
    Krastm1Mok/Kras+
Nrastm1Mok/Nrastm1Mok

involves: 129S/SvEv * C57BL/6 * DBA/2
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
homeostasis/metabolism
• 6 of 6 mice develop chylous ascites unlike wild-type mice