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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ciitatm1Ccum
targeted mutation 1, Cheong-Hee Chang
MGI:1857456
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Ciitatm1Ccum/Ciitatm1Ccum involves: 129S2/SvPas MGI:3663795
hm2
Ciitatm1Ccum/Ciitatm1Ccum involves: 129S2/SvPas * C57BL/6 MGI:3844894
hm3
Ciitatm1Ccum/Ciitatm1Ccum involves: 129S2/SvPas * C57BL/6J MGI:3617399
hm4
Ciitatm1Ccum/Ciitatm1Ccum NOD.129S2(B6)-Ciitatm1Ccum MGI:3617533
cx5
B2mtm1Unc/B2mtm1Unc
Ciitatm1Ccum/Ciitatm1Ccum
NOD.Cg-B2mtm1Unc Ciitatm1Ccum MGI:3620137


Genotype
MGI:3663795
hm1
Allelic
Composition
Ciitatm1Ccum/Ciitatm1Ccum
Genetic
Background
involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ciitatm1Ccum mutation (4 available); any Ciita mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• no defects in hematopoiesis is observed in these mutants
• transplanted bone marrow cells show similar clonogenic potential to wild-type cells: hematopoietic recovery of irradiated wild-type mice transplanted with wild-type of C2ta-null bone marrow cells is similar




Genotype
MGI:3844894
hm2
Allelic
Composition
Ciitatm1Ccum/Ciitatm1Ccum
Genetic
Background
involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ciitatm1Ccum mutation (4 available); any Ciita mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• class II surface expression in the cortex and medulla of the thymus is very patchy
• unactivated or activated B cells do not express class II on the surface
• class II expression is absent on CD11c+ dendritic cells




Genotype
MGI:3617399
hm3
Allelic
Composition
Ciitatm1Ccum/Ciitatm1Ccum
Genetic
Background
involves: 129S2/SvPas * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ciitatm1Ccum mutation (4 available); any Ciita mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• T cells proliferate poorly in response to keyhole limpet hemocyanin stimulation compared to wild-type

immune system
• mice have reduced numbers of mature CD4+ T cells in the periphery
• in spleen cells, there is a 4.5-fold increase of CD4+CD69high cells in mutants at 8 weeks after parasite infection
• in spleen cell, a slight increase in the number of CD8+ T cells compared to wild-type is seen pre- and post-infection
• the expression of IL-10 after stimulation with LPS of IL-4 for 3 days is enhanced in mutant B cells by about 2-3 fold
• T cells proliferate poorly in response to keyhole limpet hemocyanin stimulation compared to wild-type
• CD4+ T cell responses are significantly reduced to allogeneic stimulation
• when day 16 fetal liver cells are transferred into wild-type mice, CD4+ T cell development is normal and when wild-type fetal cells are transferred into homozygotes, CD4+ T cells fail to mature
• total numbers of cells in lymph nodes of null increased only slightly after infection with L. amazonensis compared with an 8-fold increase in size of wild-type
• Bone marrow-derived mutant dendritic cells in culture differentiate in response to GM-CSF despite absence of MHC class II molecules; however, when stimulated with LPS of CpG display about a 2-fold increase in IL-10 expression compared to wild-type
• MHC class II molecules are not detected in organs of mutants, except for interdigitating reticular cells where expression is not induced by interferon-gamma injection (J:31601)
• bone marrow-derived dendritic cells do not express MHC class II molecules (J:96421)
• macrophages do not express MHC II cell surface expression on interferon-gamma stimulation
• spleen and lymph node cells produced no measurable cytokines at 8 weeks after infection
• after infection with L. Amazonensis, mice show no lesion development up to 11 weeks post infection, reduced cellular infiltration, lower parasite load and few cells in the popliteal lymph nodes draining the foot
• after infection with L. amazonensis, infected cells contained tightly packed organisms with few parasitophorous vacuole visible whereas infected macrophages in wild-type mice contained only a few organisms which are preferentially located at the edge of parasitophorous vacuoles
• lesions become significantly larger with higher parasitic burdens 16 weeks after infection with L. major, compared to eventual lesion resolution in wild-type mice

hematopoietic system
• mice have reduced numbers of mature CD4+ T cells in the periphery
• in spleen cells, there is a 4.5-fold increase of CD4+CD69high cells in mutants at 8 weeks after parasite infection
• in spleen cell, a slight increase in the number of CD8+ T cells compared to wild-type is seen pre- and post-infection
• the expression of IL-10 after stimulation with LPS of IL-4 for 3 days is enhanced in mutant B cells by about 2-3 fold
• T cells proliferate poorly in response to keyhole limpet hemocyanin stimulation compared to wild-type
• CD4+ T cell responses are significantly reduced to allogeneic stimulation
• when day 16 fetal liver cells are transferred into wild-type mice, CD4+ T cell development is normal and when wild-type fetal cells are transferred into homozygotes, CD4+ T cells fail to mature
• macrophages do not express MHC II cell surface expression on interferon-gamma stimulation

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
MHC class II deficiency DOID:5812 OMIM:209920
J:31601




Genotype
MGI:3617533
hm4
Allelic
Composition
Ciitatm1Ccum/Ciitatm1Ccum
Genetic
Background
NOD.129S2(B6)-Ciitatm1Ccum
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ciitatm1Ccum mutation (4 available); any Ciita mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• on the NOD background, by 35 weeks of age and later, no mutants show hyperglycemia while 40% of NOD-sufficient mice develop diabetes by 35 weeks of age

immune system
• a 10-fold reduction in levels of peripheral CD4+ T cells is observed compared to controls
• CD8+ T cells are slightly over-represented in mutants (25%) compared to controls (16%)
• MHC class II molecule expression is almost completely absent from spleen cells
• mice do not develop diabetes (2 consecutive blood glucose measures >16.5 nM) up to 30 weeks of age
• female mutants show pancreatic infiltration by 15 weeks of age
• when recovered from NOD diabetic female recipients, islet grafts obtained from these class II-deficient NOD mice show severe infiltration and lack almost all insulin production
• at 15 weeks of age, around 2% of islets in female mutants show perinsulitis or insulitis compared with 75% in pre-diabetic and diabetic mice

hematopoietic system
• a 10-fold reduction in levels of peripheral CD4+ T cells is observed compared to controls
• CD8+ T cells are slightly over-represented in mutants (25%) compared to controls (16%)

endocrine/exocrine glands
• female mutants show pancreatic infiltration by 15 weeks of age
• when recovered from NOD diabetic female recipients, islet grafts obtained from these class II-deficient NOD mice show severe infiltration and lack almost all insulin production
• at 15 weeks of age, around 2% of islets in female mutants show perinsulitis or insulitis compared with 75% in pre-diabetic and diabetic mice

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
NOT type 1 diabetes mellitus DOID:9744 OMIM:222100
J:106081




Genotype
MGI:3620137
cx5
Allelic
Composition
B2mtm1Unc/B2mtm1Unc
Ciitatm1Ccum/Ciitatm1Ccum
Genetic
Background
NOD.Cg-B2mtm1Unc Ciitatm1Ccum
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B2mtm1Unc mutation (38 available); any B2m mutation (64 available)
Ciitatm1Ccum mutation (4 available); any Ciita mutation (8 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• NOD diabetic female mice which receive islet grafts from these classI/II-deficient NOD mice remain euglycemic for up to 147 days, compared to mice receiving islet grafts from NOD or class II-deficient NOD mice which maintain euglycemia for only 6-8 days after transplant; upon removal of the graft-bearing kidney, hyperglycemia returned within 24 hours

hematopoietic system
• a significant reduction of CD4+ and CD8+ T cells in the periphery is observed in these animals

immune system
• a significant reduction of CD4+ and CD8+ T cells in the periphery is observed in these animals
• mice monitored to 30 weeks of age do not develop type I diabetes (2 consecutive blood glucose readings >16.5nM) while 70% of wild-type NOD mice develop diabetes

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
NOT type 1 diabetes mellitus DOID:9744 OMIM:222100
J:107051





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory