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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Apobtm2Sgy
targeted mutation 2, Stephen G Young
MGI:1857304
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Apobtm2Sgy/Apobtm2Sgy involves: 129/Sv * C57BL/6 MGI:2661996
ht2
Apobtm2Sgy/Apobtm4Sgy involves: 129/Sv * 129S4/SvJae * C57BL/6 MGI:2672089
cx3
Apobtm2Sgy/Apobtm2Sgy
Apoetm1Unc/Apoetm1Unc
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 MGI:5882611
cx4
Apobtm2Sgy/Apobtm2Sgy
Apoetm1Unc/Apoetm1Unc
Lepob/Lepob
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 MGI:5819053
cx5
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Lepob/Lepob
involves: 129S7/SvEvBrd * C57BL/6 MGI:5819052
cx6
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
involves: 129S7/SvEvBrd * C57BL/6 MGI:4367110
cx7
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J * SJL MGI:5771865
cx8
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
involves: 129S7/SvEvBrd * C57BL/6J MGI:5771869
cx9
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
involves: 129S7/SvEvBrd * C57BL/6 * SJL MGI:4367111


Genotype
MGI:2661996
hm1
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism




Genotype
MGI:2672089
ht2
Allelic
Composition
Apobtm2Sgy/Apobtm4Sgy
Genetic
Background
involves: 129/Sv * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Apobtm4Sgy mutation (0 available); any Apob mutation (97 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism




Genotype
MGI:5882611
cx3
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Apoetm1Unc/Apoetm1Unc
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Apoetm1Unc mutation (23 available); any Apoe mutation (77 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• mice exhibit fewer atherosclerotic lesions in aortas and smaller lesions in the aortic root than single Apoetm1Unc homozygotes
• extent of atherosclerosis correlates with plasma cholesterol levels

growth/size/body
• mice are slightly, but significantly lighter than single Apoetm1Unc homozygotes at 200 days of age

homeostasis/metabolism
• triglyceride levels are higher than in single Apoetm1Unc homozygotes
• the VLDL is more enriched in triglyceride than the VLDL from double Apobtm1Sgy Apoetm1Unc homozygotes
• mean size of VLDL is larger than that of the VLDL from single Apoetm1Unc homozygotes or double Apobtm1Sgy Apoetm1Unc homozygotes
• the IDL and LDL particles are larger than that from single Apoetm1Unc homozygotes or double Apobtm1Sgy Apoetm1Unc homozygotes
• HDL cholesterol levels at 200 days of age are slightly, but significantly, higher than in single Apoetm1Unc homozygotes or double Apobtm1Sgy Apoetm1Unc homozygotes
• very high levels of VLDL cholesterol
• total cholesterol levels are lower at 7 weeks, 3 and 6 months, and 200 days of age compared to single Apoetm1Unc homozygotes or double Apobtm1Sgy Apoetm1Unc homozygotes
• plasma cholesterol levels decrease with time




Genotype
MGI:5819053
cx4
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Apoetm1Unc/Apoetm1Unc
Lepob/Lepob
Genetic
Background
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Apoetm1Unc mutation (23 available); any Apoe mutation (77 available)
Lepob mutation (6 available); any Lep mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• at 12 weeks of age, atherosclerotic lesions are seen mainly in the aortic region of the whole aorta
• leptin treatment, but not food restriction, results in lower atherosclerotic lesion size
• elevated blood pressure
• treatment with enalapril corrects the elevated blood pressure

growth/size/body
• increase in body weight, with mice weighing about 63 grams at 15-16 weeks of age

homeostasis/metabolism
• mice are hyperglycemic and glycemic control fluctuates with age
• mice show improved in blood glucose with food restriction but not with leptin treatment
• increase in blood insulin levels
• neither food restriction or leptin treatment has an effect on insulin level
• mice have higher VLDL levels than LDL, with lower HDL levels compared to wild-type mice which have HDL as the dominant lipoprotein species and very low levels of LDL and VLDL
• mice have lower HDL levels
• cholesterol levels are elevated almost 10-fold compared to wild-type mice
• both leptin treatment and food restriction result in 52.6% and nonsignificant 18.5% reduction, respectively, of cholesterol levels
• mice have higher VLDL levels than LDL levels
• plasma triglyceride levels are 4-fold higher at 15-16 weeks of age than in wild-type mice
• neither food restriction or leptin treatment has an effect on plasma triglyceride levels
• glucose intolerance is evident at 7-8 weeks of age and sustained until 15-16 weeks

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
metabolic syndrome X DOID:14221 OMIM:605552
J:133453




Genotype
MGI:5819052
cx5
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Lepob/Lepob
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (51 available)
Lepob mutation (6 available); any Lep mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• at 12 weeks of age, atherosclerotic lesions are seen mainly in the aortic region of the whole aorta
• leptin treatment and food restriction results in lower atherosclerotic lesion size
• elevated blood pressure
• treatment with enalapril corrects the elevated blood pressure

growth/size/body
• increase in body weight, with mice weighing about 58 grams at 15-16 weeks of age

homeostasis/metabolism
• mice show increased insulin levels
• however, mice show normal glucose levels and glucose tolerance
• leptin treatment lowers insulin more than food restriction does
• mice have more LDL than VLDL, with lower HDL levels compared to wild-type mice which have HDL as the dominant lipoprotein species and very low levels of LDL and VLDL
• both leptin treatment and food restriction result in 39.2% and 30% reduction, respectively, of cholesterol levels
• mice have lower HDL levels
• cholesterol levels are elevated almost 10-fold compared to wild-type mice
• mice have more LDL than VLDL
• plasma triglyceride levels are 3-fold higher at 15-16 weeks of age than in wild-type mice
• both leptin treatment and food restriction result in a 32% and 58%, respectively, reduction in circulating triglyceride levels

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
metabolic syndrome X DOID:14221 OMIM:605552
J:133453




Genotype
MGI:4367110
cx6
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (51 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• age related accumulation of esterified cholesterol in the basement of the retinal pigment epithelial layer
• losses in rod and cone sensitivity only after 14 months of age

pigmentation
• age related accumulation of esterified cholesterol in the basement of the retinal pigment epithelial layer




Genotype
MGI:5771865
cx7
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (51 available)
Tg(Ins-Igf2)1Fbos mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• fasting glycemia is increased on a standard chow diet
• mice develop diabetes mellitus based on fasting hyperglycemia and lack of compensatory increase in insulin secretion when fed a high-fat/sucrose/cholesterol (HFSC) diet for 6 months
• increase in fasting insulin levels on standard chow diet
• mice exhibit cholesterolemia on both a standard chow diet and a high-fat/sucrose/cholesterol (HFSC) diet

cardiovascular system
• mice fed the HFSC diet show higher expression of osteogenic genes in the aortic root
• mice fed the HFSC diet show aortic root fibrosis
• HFSC-fed mice exhibit increased expression of hypertrophic cardiac markers
• increase in left ventricular mass on both a chow and HFSC diet
• left ventricular hypertrophy in HFSC-fed mice
• aortic valve area is decreased in HFSC-fed mice
• mice fed the HFSC diet show aortic valve fibrosis
• 80% of mice fed the HFSC diet for 6 months develop aortic stenosis, with mice showing increased peak aortic jet velocity, peak transvalvular pressure gradient, and mean transvalvular pressure gradient; magnitude of aortic stenosis is greater than in double Apob and Ldlr homozygotes
• HFSC-fed mice exhibit aortic valve calcification
• total ventricular weight corrected for body weight is elevated in mice fed the HFSC diet
• when corrected for tibia length, the total ventricular weight is increased in mice either on the chow or HFSC diet
• mice fed the HFSC diet show aortic root fibrosis and aortic valve fibrosis
• echocardiography shows impaired left ventricular function in mice fed a chow diet with worsening cardiac dysfunction on the HFSC diet
• the mitral E/E ratio (mitral inflow measured by pulsed-wave over tissue Doppler) is increased in mice on the HFSC diet
• isovolumic relaxation time, corrected for heart rate, is decreased in mice on the HFSC diet
• cardiac output is increased in mice fed the HFSC diet
• stroke volume is increased in mice fed the HFSC diet
• some areas of the aortic surface leaflet from HFSC-fed mice are denuded of endothelial cells and show presence of inflammatory cell aggregates, platelets, and fibrin covering the extracellular matrix
• reduction in systolic fractional shortening in mice fed the HFSC diet

immune system
• some areas of the aortic surface leaflet from HFSC-fed mice are denuded of endothelial cells and show presence of inflammatory cell aggregates, platelets, and fibrin covering the extracellular matrix

muscle
• reduction in systolic fractional shortening in mice fed the HFSC diet

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus 2 DOID:0110741 OMIM:125852
J:227165




Genotype
MGI:5771869
cx8
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (51 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• mice fed the HFSC diet show higher expression of osteogenic genes in the aortic root
• 40% of mice fed the HFSC diet for 6 months develop aortic stenosis, with mice showing increased peak aortic jet velocity, peak transvalvular pressure gradient, and mean transvalvular pressure gradient
• total ventricular weight corrected for body weight is elevated in mice fed the HFSC diet
• however, when corrected for tibia length, the total ventricular weight is no longer elevated
• cardiac output is increased in mice fed the HFSC diet
• stroke volume is increased in mice fed the HFSC diet
• reduction in systolic fractional shortening in mice fed the HFSC diet

homeostasis/metabolism
• increase in fasting insulin levels on standard chow diet
• however, mice show normal fasting glucose levels on standard chow diet
• mice exhibit cholesterolemia on both a standard chow diet and a high-fat/sucrose/cholesterol (HFSC) diet

muscle
• reduction in systolic fractional shortening in mice fed the HFSC diet




Genotype
MGI:4367111
cx9
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (97 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (51 available)
Tg(Ins-Igf2)1Fbos mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• elevated relative to mice lacking the transgene at 6 months regardless of diet
• difference from controls lacking the transgene persists on a high fat diet but not on a normal diet at 15 months
• plasma insulin levels are 2X control levels at 6 months in fed mice and fasted mice on a high fat diet
• fasted mice on a normal diet do not show elevated insulin
• reduced insulin sensitivity over the course of an insulin tolerance test
• sustained elevation of blood glucose during a glucose tolerance test regardless of diet
• blood insulin is unchanged over the course of a glucose tolerance test

cardiovascular system
• lesion thickness increases more on a high fat diet than does lesion area
• increased calcification of lesions after 15 months on a high fat diet, particularly for females





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last database update
04/18/2017
MGI 6.08
The Jackson Laboratory