About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Apobtm2Sgy
targeted mutation 2, Stephen G Young
MGI:1857304
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Apobtm2Sgy/Apobtm2Sgy involves: 129/Sv * C57BL/6 MGI:2661996
ht2
Apobtm2Sgy/Apobtm4Sgy involves: 129/Sv * 129S4/SvJae * C57BL/6 MGI:2672089
cx3
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
involves: 129S7/SvEvBrd * C57BL/6 MGI:4367110
cx4
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J * SJL MGI:5771865
cx5
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
involves: 129S7/SvEvBrd * C57BL/6J MGI:5771869
cx6
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
involves: 129S7/SvEvBrd * C57BL/6 * SJL MGI:4367111


Genotype
MGI:2661996
hm1
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Genetic
Background
involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (86 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism




Genotype
MGI:2672089
ht2
Allelic
Composition
Apobtm2Sgy/Apobtm4Sgy
Genetic
Background
involves: 129/Sv * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (86 available)
Apobtm4Sgy mutation (0 available); any Apob mutation (86 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism

Mouse Models of Human Disease
OMIM ID Ref(s)
Apolipoprotein B; APOB 107730 J:46549




Genotype
MGI:4367110
cx3
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (86 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (48 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
vision/eye
• age related accumulation of esterified cholesterol in the basement of the retinal pigment epithelial layer
• losses in rod and cone sensitivity only after 14 months of age

pigmentation
• age related accumulation of esterified cholesterol in the basement of the retinal pigment epithelial layer




Genotype
MGI:5771865
cx4
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (86 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (48 available)
Tg(Ins-Igf2)1Fbos mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• fasting glycemia is increased on a standard chow diet
• mice develop diabetes mellitus based on fasting hyperglycemia and lack of compensatory increase in insulin secretion when fed a high-fat/sucrose/cholesterol (HFSC) diet for 6 months
• increase in fasting insulin levels on standard chow diet
• mice exhibit cholesterolemia on both a standard chow diet and a high-fat/sucrose/cholesterol (HFSC) diet

cardiovascular system
• mice fed the HFSC diet show higher expression of osteogenic genes in the aortic root
• mice fed the HFSC diet show aortic root fibrosis
• HFSC-fed mice exhibit increased expression of hypertrophic cardiac markers
• increase in left ventricular mass on both a chow and HFSC diet
• left ventricular hypertrophy in HFSC-fed mice
• aortic valve area is decreased in HFSC-fed mice
• mice fed the HFSC diet show aortic valve fibrosis
• 80% of mice fed the HFSC diet for 6 months develop aortic stenosis, with mice showing increased peak aortic jet velocity, peak transvalvular pressure gradient, and mean transvalvular pressure gradient; magnitude of aortic stenosis is greater than in double Apob and Ldlr homozygotes
• HFSC-fed mice exhibit aortic valve calcification
• total ventricular weight corrected for body weight is elevated in mice fed the HFSC diet
• when corrected for tibia length, the total ventricular weight is increased in mice either on the chow or HFSC diet
• mice fed the HFSC diet show aortic root fibrosis and aortic valve fibrosis
• echocardiography shows impaired left ventricular function in mice fed a chow diet with worsening cardiac dysfunction on the HFSC diet
• the mitral E/E ratio (mitral inflow measured by pulsed-wave over tissue Doppler) is increased in mice on the HFSC diet
• isovolumic relaxation time, corrected for heart rate, is decreased in mice on the HFSC diet
• cardiac output is increased in mice fed the HFSC diet
• stroke volume is increased in mice fed the HFSC diet
• some areas of the aortic surface leaflet from HFSC-fed mice are denuded of endothelial cells and show presence of inflammatory cell aggregates, platelets, and fibrin covering the extracellular matrix
• reduction in systolic fractional shortening in mice fed the HFSC diet

immune system
• some areas of the aortic surface leaflet from HFSC-fed mice are denuded of endothelial cells and show presence of inflammatory cell aggregates, platelets, and fibrin covering the extracellular matrix

muscle
• reduction in systolic fractional shortening in mice fed the HFSC diet

Mouse Models of Human Disease
OMIM ID Ref(s)
Diabetes Mellitus, Insulin-Dependent, 2 125852 J:227165




Genotype
MGI:5771869
cx5
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (86 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (48 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• mice fed the HFSC diet show higher expression of osteogenic genes in the aortic root
• 40% of mice fed the HFSC diet for 6 months develop aortic stenosis, with mice showing increased peak aortic jet velocity, peak transvalvular pressure gradient, and mean transvalvular pressure gradient
• total ventricular weight corrected for body weight is elevated in mice fed the HFSC diet
• however, when corrected for tibia length, the total ventricular weight is no longer elevated
• cardiac output is increased in mice fed the HFSC diet
• stroke volume is increased in mice fed the HFSC diet
• reduction in systolic fractional shortening in mice fed the HFSC diet

homeostasis/metabolism
• increase in fasting insulin levels on standard chow diet
• however, mice show normal fasting glucose levels on standard chow diet
• mice exhibit cholesterolemia on both a standard chow diet and a high-fat/sucrose/cholesterol (HFSC) diet

muscle
• reduction in systolic fractional shortening in mice fed the HFSC diet




Genotype
MGI:4367111
cx6
Allelic
Composition
Apobtm2Sgy/Apobtm2Sgy
Ldlrtm1Her/Ldlrtm1Her
Tg(Ins-Igf2)1Fbos/?
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apobtm2Sgy mutation (4 available); any Apob mutation (86 available)
Ldlrtm1Her mutation (19 available); any Ldlr mutation (48 available)
Tg(Ins-Igf2)1Fbos mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• elevated relative to mice lacking the transgene at 6 months regardless of diet
• difference from controls lacking the transgene persists on a high fat diet but not on a normal diet at 15 months
• plasma insulin levels are 2X control levels at 6 months in fed mice and fasted mice on a high fat diet
• fasted mice on a normal diet do not show elevated insulin
• reduced insulin sensitivity over the course of an insulin tolerance test
• sustained elevation of blood glucose during a glucose tolerance test regardless of diet
• blood insulin is unchanged over the course of a glucose tolerance test

cardiovascular system
• lesion thickness increases more on a high fat diet than does lesion area
• increased calcification of lesions after 15 months on a high fat diet, particularly for females





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
06/22/2016
MGI 6.04
The Jackson Laboratory