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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Serpine1tm1Mlg
targeted mutation 1, Richard C Mulligan
MGI:1857287
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Serpine1tm1Mlg/Serpine1tm1Mlg B6.129S2-Serpine1tm1Mlg/J MGI:3690223
hm2
Serpine1tm1Mlg/Serpine1tm1Mlg involves: 129S2/SvPas MGI:5763490
hm3
Serpine1tm1Mlg/Serpine1tm1Mlg involves: 129S2/SvPas * C57BL/6 MGI:3037601
hm4
Serpine1tm1Mlg/Serpine1tm1Mlg involves: 129S2/SvPas * C57BL/6 * C57BLKS/J MGI:3803205
cx5
Apoetm1Unc/Apoetm1Unc
Serpine1tm1Mlg/Serpine1tm1Mlg
B6.129-Serpine1tm1Mlg Apoetm1Unc MGI:3811066
cx6
Ldlrtm1Her/Ldlrtm1Her
Serpine1tm1Mlg/Serpine1tm1Mlg
B6.129S-Serpine1tm1Mlg Ldlrtm1Her MGI:3811068
cx7
Serpinb2tm1Dgi/Serpinb2tm1Dgi
Serpine1tm1Mlg/Serpine1tm1Mlg
involves: 129S1/Sv * 129S2/SvPas * C57BL/6 * DBA/2 MGI:4837628
cx8
Lepob/Lepob
Serpine1tm1Mlg/Serpine1tm1Mlg
involves: 129S2/SvPas * C57BL/6 MGI:3037602
cx9
Leprdb/Leprdb
Serpine1tm1Mlg/Serpine1tm1Mlg
involves: 129S2/SvPas * C57BL/6 * C57BLKS/J MGI:3803207
cx10
Serpinb2tm2Dgi/Serpinb2tm2Dgi
Serpine1tm1Mlg/Serpine1tm1Mlg
involves: 129S2/SvPas * C57BL/6J * DBA/2J MGI:4837627


Genotype
MGI:3690223
hm1
Allelic
Composition
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
B6.129S2-Serpine1tm1Mlg/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• aortic vessel explants show increased capillary sprouting in both collagen lattices and Matrigel
• older mutants develop cardiac fibrosis, however fibrosis is not seen in the liver, spleen, lungs or kidneys and do not develop small vessel disease
• older mutants exhibit significantly more macrophages in the heart

immune system
• older mutants exhibit significantly more macrophages in the heart
• following infection with live Streptococcus pneumoniae, S. pneumoniae wild-type lysate, and S. pneumoniae pneumolysin (PLY), mice begin to die earlier and are all dead by 5 days post-infection
• following infection with S. pneumoniae, acute lung injury and alveolar hemorrhage is enhanced
• following infection with S. pneumoniae strain ATCC 6303 (a more virulent strain), mortality is increased (10% survival compared to 45% in wild-type mice)

homeostasis/metabolism
• 3 weeks after BDL, urokinase plasminogen activator (uPA) and plasmin activity are not increased in mutants but tissue-type plasminogen activator (tPA) activity is significantly increased relative to wild-type
• 3 weeks after BDL, pro-MMP-9 and MMP-9 activities in liver homogenates from mutants are increased significantly compared to wild-type controls

liver/biliary system
N
• stellate cell activation, TGFbeta-1 and collagen synthesis are similar in mutants and controls before and after bile duct ligation
• 3 weeks after bile duct ligation (BDL), hepatic fibrosis is reduced 18-26% compared to wild-type; hepatic collagen content is similar to sham-operated wild-type

mortality/aging
• following infection with live Streptococcus pneumoniae, S. pneumoniae wild-type lysate, and S. pneumoniae pneumolysin (PLY), mice begin to die earlier and are all dead by 5 days post-infection
• following infection with S. pneumoniae strain ATCC 6303 (a more virulent strain), mortality is increased (10% survival compared to 45% in wild-type mice)




Genotype
MGI:5763490
hm2
Allelic
Composition
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

immune system
• LPS-treated mice exhibit increased survival compared with wild-type mice

homeostasis/metabolism




Genotype
MGI:3037601
hm3
Allelic
Composition
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• less weight gain on a high fat diet than normal
• at 2 weeks, mutants remain below initial body weight after turpentine injection-induced weight loss but wild-type mice recover and begin to gain weight around 6-7 days after injection
• turpentine injection induces significant weight loss in mutants and wild-type mice in initial 2 days; however, after 2 days, wild-type animals start to recover body weight whereas mutants do not

immune system
N
• chemokine levels in injured tissue are similar between mutants and controls, peaking at 1-3 days
• relative to controls following turpentine injection
• local Il6 levels in injured tissue at 8 hours post-injection are lower than in controls
• plasma levels are markedly reduced at 8 hours after turpentine injection
• in mutants after turpentine injection, serum amyloid P and C3 are significantly lower at 24 hours compared to wild-type and display a delayed peak at 3 days after injection
• C3 peak levels at day 3 are higher than in wild-type
• haptoglobin levels do not differ from wild-type at any time after turpentine injection
• after turpentine injection to hind limb to induce tissue injury, 8 week-old mutants show significantly more local tissue injury compared to controls
• treatment resulted in local abscess formation and neutrophilic granulocyte infiltration encapsulated by macrophages and fibroblasts
• increased mortality
• higher incidence of fibrin positive crescents
• increased infiltration of injury with CD4+ T cells
• increased thrombin production
• inflammation induced fibrosis in the lung occurs at a much slower rate
• survival is better
• fibrin content of lungs reduced generally

renal/urinary system
• an earlier enhancement of protein urea
• higher incidence of fibrin positive crescents
• increased infiltration of injury with CD4+ T cells
• increased mortality
• increased thrombin production
• higher incidence of fibrin positive crescents

cardiovascular system
• neovascularization in general is reduced
• injury response in carotid arteries is reduced although cell proliferation in the adventitia is increased
• no fibrin present in the injured vessel walls

hematopoietic system
N
• bleeding time was normal
• relative to controls following turpentine injection

respiratory system
• inflammation induced fibrosis in the lung occurs at a much slower rate
• survival is better
• fibrin content of lungs reduced generally
• no significant bleomycin-induced increase in lung collagen content is detected at 3 weeks posttreatment whereas treated wild-type mice show a significant increase in lung collagen; heterozygous mice are intermediate between null and wild-type in terms of collagen content
• lung fibrin deposition following bleomycin treatment is almost undetectable compared to treated wild-type mice

adipose tissue
• subcutaneous white fat pad mass was also reduced
• after 12 weeks on a high fat diet, epididymal fat pad was only 25% of wild-type

neoplasm
N
• no significant differences are observed compared to controls in terms of primary tumor size of footpad tumors induced by foot pad injection with tumor cells at 10, 17, or 21 days post-injection
• after injection of melanoma cells, number of primary pulmonary metastases post-injection, or tumor burdens initiated by injections of increasing doses of cells do not significantly differ from wild-type controls
• survival times after foot pad tumor removal are not significantly different among Serpine1-deficient, Serpine1-overexpressing transgenic, or wild-type mice

homeostasis/metabolism
• increased on a high fat diet
• increased after 12 weeks on a high fat diet
• after 12 weeks on a high fat diet, oxygen consumption is increased
• lower levels in both skeletal muscle and liver after 12 weeks on a high fat diet
• lower levels after 12 weeks on a high fat diet
• local Il6 levels in injured tissue at 8 hours post-injection are lower than in controls
• plasma levels are markedly reduced at 8 hours after turpentine injection
• an earlier enhancement of protein urea
• after 8 hours, myeloperoxidase (MPO) activity is higher in mutants in hind limb homogenates than in wild-type
• dermal incisions healed more rapidly than normal
• thickened epidermal layer found within newly healed wounds

liver/biliary system
• lower levels after 12 weeks on a high fat diet

integument
• subcutaneous white fat pad mass was also reduced




Genotype
MGI:3803205
hm4
Allelic
Composition
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
involves: 129S2/SvPas * C57BL/6 * C57BLKS/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
N
• mice do not develop significant albuminuria after 6 months of streptozotocin (STZ) -induced diabetes
• STZ-treated mutants show elevated BUN levels compared to untreated mice, but levels are comparable to STZ-treated wild-type
• in streptozotocin-treated (STZ) mice (to induce diabetes), serum glucose is significantly increased compared to untreated mutant or wild-type controls
• mean levels are significantly lower than in wild-type controls and STZ-treated mutants

renal/urinary system
• total kidney collagen in STZ-treated mice increases only 8% compared to 40% in treated wild-type
• glomerular extracellular matrix (ECM) area is greater than in wild-type controls; however, when expressed as fractional glomerular ECM area, little difference is observed between any genotype
• glomerular extracellular matrix (ECM) area is significantly higher compared to controls; after 6 months of STZ-induced diabetes, ECM area is decreased 15% compared to Serpine1 single deficient mice while in STZ-treated wild-type mice, ECM area is increased 64% relative to untreated wild-type mice




Genotype
MGI:3811066
cx5
Allelic
Composition
Apoetm1Unc/Apoetm1Unc
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
B6.129-Serpine1tm1Mlg Apoetm1Unc
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apoetm1Unc mutation (31 available); any Apoe mutation (97 available)
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• when fed a high fat Western diet for 6-30 weeks, mice develop atherosclerotic aortic lesions of the intimal and medial areas; incidence and severity are similar for Serpine1-deficient or transgenic Apoe-deficient genotypes
• lesions are somewhat larger than those observed on the Ldlr-deficient background
• cellular composition of lesions is similar among Serpine-deficient or transgenic, Apoe-deficient genotypes, or Apoe-deficient only mice; at early time points, a greater foam cell content is observed, with more prominent cholesterol clefts and necrotic areas evident with increasing age
• fibrin deposition is not significantly different among Serpine-deficient or transgenic, Apoe-deficient genotypes, or Apoe-deficient only mice

homeostasis/metabolism
• mice develop severe cholesterolemia when receiving a high fat diet from 6 to 30 weeks (elevated levels are detected at 6, 15, and 30 weeks)




Genotype
MGI:3811068
cx6
Allelic
Composition
Ldlrtm1Her/Ldlrtm1Her
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
B6.129S-Serpine1tm1Mlg Ldlrtm1Her
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ldlrtm1Her mutation (20 available); any Ldlr mutation (36 available)
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• when fed a high fat Western diet for 6-30 weeks, mice develop atherosclerotic aortic lesions of the intimal and medial areas; incidence and severity are similar for Serpine1-deficient or transgenic Ldlr-deficient genotypes

homeostasis/metabolism
• mice develop severe cholesterolemia when receiving a high fat diet from 6 to 30 weeks (elevated levels are detected at 6, 15, and 30 weeks)




Genotype
MGI:4837628
cx7
Allelic
Composition
Serpinb2tm1Dgi/Serpinb2tm1Dgi
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
involves: 129S1/Sv * 129S2/SvPas * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Serpinb2tm1Dgi mutation (1 available); any Serpinb2 mutation (20 available)
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
N
• mice exhibit normal wound healing




Genotype
MGI:3037602
cx8
Allelic
Composition
Lepob/Lepob
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
involves: 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lepob mutation (5 available); any Lep mutation (16 available)
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• serum insulin level was only moderately elevated from normal mice
• glucose administration caused only a moderate additional
• levels normal at birth but rise moderately with age

immune system
• levels normal at birth but rise moderately with age




Genotype
MGI:3803207
cx9
Allelic
Composition
Leprdb/Leprdb
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
involves: 129S2/SvPas * C57BL/6 * C57BLKS/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Leprdb mutation (12 available); any Lepr mutation (83 available)
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• 23% of females and 44% of males in the F3 generation died prematurely, of indeterminate cause

growth/size/body
• female mice are obese relative to wild-type and Serpine1-deficient single mutant controls
• 63% of males in F3 generation are runts with body weight ranging from 20-40 grams compared to 60-100 grams for littermates not displaying runt phenotype

renal/urinary system
• present with slightly higher urinary albumin to creatinine ratios relative to Lepr single homozygotes
• total kidney collagen is increased in females by 47% compared to female single Serpine1-deficient animals
• glomerular extracellular matrix (ECM) areas are reduced 10% in females compared to female single Serpine1-deficient animals; however, when expressed as fractional glomerular ECM area, little difference is observed between any genotype

homeostasis/metabolism
• significantly higher BUN levels compared to Serpine1-deficient single mutants
• hyperglycemia is less severe than observed in Serpine1-sufficient, Lepr-deficient controls at 10, 14, and 34 weeks of age
• present with slightly higher urinary albumin to creatinine ratios relative to Lepr single homozygotes
• total urokinase plasminogen activator (uPA) activity is significantly higher than wild-type compared to Serpine1-sufficient diabetic controls




Genotype
MGI:4837627
cx10
Allelic
Composition
Serpinb2tm2Dgi/Serpinb2tm2Dgi
Serpine1tm1Mlg/Serpine1tm1Mlg
Genetic
Background
involves: 129S2/SvPas * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Serpinb2tm2Dgi mutation (0 available); any Serpinb2 mutation (20 available)
Serpine1tm1Mlg mutation (4 available); any Serpine1 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
N
• mice exhibit normal wound healing





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last database update
02/11/2020
MGI 6.14
The Jackson Laboratory