Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Srctm1Sor mutation
(2 available);
any
Src mutation
(144 available)
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reproductive system
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• ovariectomized female homozygotes treated with a low dose of estrogen (E2) and a high dose of progesterone (P4), followed by mechanical stimulation of the left uterine horn, showed little or no detectable decidual responses relative to similarly-treated wild-type and heterozygous females, as determined by uterine histology and uterine weight changes
• in vitro, endometrial stromal cells (ESCs) from wild-type mice became morphologically decidualized when cultured in the presence of E2+P4 for 5 days, whereas ESCs from female homozygotes remained morphologically unchanged
• however, prolonged treatment with E2+P4 for 10 days elicited both functional and morphological decidualization even in homozygous mutant ESCs
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• female homozygotes are severely subfertile or infertile, partly due to impaired decidualization resulting from the loss of maternal function in endometrial stromal cells
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embryo
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• ovariectomized female homozygotes treated with a low dose of estrogen (E2) and a high dose of progesterone (P4), followed by mechanical stimulation of the left uterine horn, showed little or no detectable decidual responses relative to similarly-treated wild-type and heterozygous females, as determined by uterine histology and uterine weight changes
• in vitro, endometrial stromal cells (ESCs) from wild-type mice became morphologically decidualized when cultured in the presence of E2+P4 for 5 days, whereas ESCs from female homozygotes remained morphologically unchanged
• however, prolonged treatment with E2+P4 for 10 days elicited both functional and morphological decidualization even in homozygous mutant ESCs
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Allelic
Composition |
Srctm1Sor/Srctm1Sor
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Genetic
Background |
either: 129S7/SvEvBrd-Srctm1Sor or (involves: 129S7/SvEvBrd * C57BL/6J) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Srctm1Sor mutation
(2 available);
any
Src mutation
(144 available)
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mortality/aging
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• most animals died at 3-4 weeks of age if weaned; one-third of mice could be maintained past 5 weeks if not weaned and kept on a soft food diet
• Background Sensitivity: mice on a mixed background survive longer than mice on the inbred 129S7/SvEvBrd genetic background
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• at 3 weeks of age, only 19% of the mice are homozygous, suggesting a partial prenatal and/or partial early postnatal lethality
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growth/size/body
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• incisors are absent at 3 weeks of age
• incisor teeth are well developed, but fail to erupt through the gum
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• some molar teeth show partial eruption, but are crowded by osseous growth of the facial bones
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• incisor teeth are well developed, but fail to erupt through the gum
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• mice have a slightly domed head
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• by 10-12 days of age, mice are noticeably smaller than controls
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• mice grow more slowly and weigh about one-third to one-half as much as controls
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skeleton
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• facial bones are thickened by fibrous and osseous tissue proliferation and lack of bone resorbtion
• smaller airway lumina are present due to abnormal facial bone morphology
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• incisors are absent at 3 weeks of age
• incisor teeth are well developed, but fail to erupt through the gum
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• some molar teeth show partial eruption, but are crowded by osseous growth of the facial bones
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• incisor teeth are well developed, but fail to erupt through the gum
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• short diaphysis of the long bones
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• thickened growth plates in the long bones
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• long bones are shorter in length
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• the bone marrow cavity was filled with little room for hematopoietic elements
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• partial absence of bone marrow
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• cortical bone is thinner and disorganized
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• the central trabeculae consist of calcified cartilage
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• there is a widening and extension of the trabecular bone deep into the distal metaphysis and diaphysis
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• noted in calvarium, nasal turbinates, facial bones, mandible, tibia and femurs; obvious in long bones at P10 and in the crainial bones at birth
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• persistence of endochondrial primary spongiosa
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• bone resorption is minimal to absent
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pigmentation
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• Background Sensitivity: mice on a mixed genetic background exhibit a lighter coat color unlike mice on an inbred 129S7/SvEvBrd genetic background
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vision/eye
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• at later ages, the eyes are frequently cloudy due to secreted material
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hematopoietic system
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N |
• normal red and white cell parameters are seen
• normal platelet counts are seen
• no evidence of hemorrhage or platelet dysfunction is seen; mice are capable of wound healing following tail or toe clipping
• the liver and spleen are normal in size
• parafollicular cells of the thyroid are normal
• Kuppfer cells in the liver are normal in quantity and morphology
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nervous system
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N |
• no microscopic brain lesions are apparent in multiple substructures
• general brain architecture is normal and axon extension and fasciculation appear normal
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immune system
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• necrotic debris and acute inflammatory cells are sometimes seen within the lumen of the nasolacrimal duct
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• suppurative periodontal inflammation is seen in some animals
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craniofacial
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• facial bones are thickened by fibrous and osseous tissue proliferation and lack of bone resorbtion
• smaller airway lumina are present due to abnormal facial bone morphology
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• incisors are absent at 3 weeks of age
• incisor teeth are well developed, but fail to erupt through the gum
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• some molar teeth show partial eruption, but are crowded by osseous growth of the facial bones
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• incisor teeth are well developed, but fail to erupt through the gum
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• mice have a slightly domed head
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limbs/digits/tail
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• enlarged, club-shaped extremities
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integument
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• Background Sensitivity: mice on a mixed genetic background exhibit a lighter coat color unlike mice on an inbred 129S7/SvEvBrd genetic background
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behavior/neurological
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• difficulty in nursing newborn pups on the occasional cases where females became pregnant
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reproductive system
mortality/aging
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• only 10% of animals survived to weaning age; normal Mendelian ratios were present at E18.5; animals did not survive past 3 weeks of age
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growth/size/body
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• animals were described as very small
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• at 3 weeks of age, animals weighed 3-4 grams compared to controls weighing 8 grams
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mortality/aging
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• some animals survived past weaning age; one survived to one year of age on a soft food diet
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• incomplete penetrance; only 10% of animals survived to weaning age; normal Mendelian ratios were present at E18.5, but many were found dead shortly after birth
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growth/size/body
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• embryos at E18.5 were approximately 25% smaller than controls despite normal placenta morphology
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respiratory system
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• animals delivered by Cesarean at E18.5 only started to breathe normally after one hour of prodding
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skeleton
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• no more severe than in homozygous Src mutant mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hcktm1Hev mutation
(0 available);
any
Hck mutation
(29 available)
Srctm1Sor mutation
(2 available);
any
Src mutation
(144 available)
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mortality/aging
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• approximately 50% die immediately after weaning
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• double homozygotes present at dramatically reduced proportions at 10-14 days of age
• ratios normal at E18.5
• deaths probably occurring perinatally
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growth/size/body
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• little weight gain from 14 to 50 days of age
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skeleton
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• proximal and distal growth zones are often fused leaving the diaphysis filled with cancellous bone
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• reduced bone marrow cavity
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• extensive development of cancellous bone reduces bone marrow cavity in long bones, vertebral bodies, sternum
• accumulations of poorly remodeled osteocartilagenous trabeculae throughout the growth zones of long bones
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• proximal and distal growth zones are often fused leaving the diaphysis filled with cancellous bone
• normal numbers of osteoclasts found
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immune system
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• accumulation of mature macrophage in bones
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• mild leucopenia at 2-4 weeks of age which is more severe at E18.5
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• reduced cell number in thymus
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• absent or poorly formed germinal centers
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• increased number of colony forming units in spleen
• greatly increased numbers of immature myeloid cells
• decreased numbers of lymphoid cells
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• reduced number of cells in lymph nodes but structure otherwise normal
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hematopoietic system
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• reduced cell number in thymus
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• mild anemia at 2-4 weeks of age which is more severe at E18.5
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• reduced number of colony forming units in bone marrow
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• accumulation of mature macrophage in bones
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• mild leucopenia at 2-4 weeks of age which is more severe at E18.5
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• absent or poorly formed germinal centers
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• increased number of colony forming units in spleen
• greatly increased numbers of immature myeloid cells
• decreased numbers of lymphoid cells
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endocrine/exocrine glands
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• reduced cell number in thymus
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Analysis Tools