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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Il2ratm1Dw
targeted mutation 1, Dennis M Willerford
MGI:1857192
Summary 7 genotypes


Genotype
MGI:3759842
hm1
Allelic
Composition		 Il2ratm1Dw/Il2ratm1Dw
Genetic
Background		 B6.129S4-Il2ratm1Dw
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

immune system
abnormal T cell activation ( J:125748 )
• over 80% of the T cells from bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit an activated phenotype
abnormal regulatory T cell physiology ( J:125748 )
• bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) die within 40-50 days of autoimmune disorders
• however, when bone marrow chimeras receive 105 CD25-positive CD4-positive wild-type T cells two weeks after reconstitution they live past six months of age without any symptoms of autoimmune disorder
• premature death can also be prevented by including 50% normal or Il2tm1Hor bone marrow during reconstitution
lymphoid hyperplasia ( J:125748 )
• some bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) have enlarged lymph nodes containing up to 3 x 108 lymphocytes

hematopoietic system
anemia ( J:125748 )
• bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit anemia
abnormal T cell activation ( J:125748 )
• over 80% of the T cells from bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit an activated phenotype
abnormal regulatory T cell physiology ( J:125748 )
• bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) die within 40-50 days of autoimmune disorders
• however, when bone marrow chimeras receive 105 CD25-positive CD4-positive wild-type T cells two weeks after reconstitution they live past six months of age without any symptoms of autoimmune disorder
• premature death can also be prevented by including 50% normal or Il2tm1Hor bone marrow during reconstitution

growth/size/body
decreased body size ( J:125748 )
• bone marrow chimeras (mutant bone marrow in Rag2-deficient hosts) exhibit decreased body weight due to an autoimmune disorder




Genotype
MGI:5883306
hm2
Allelic
Composition		 Il2ratm1Dw/Il2ratm1Dw
Genetic
Background		 B6.129S4-Il2ratm1Dw/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal salivary gland morphology ( J:125129 )
• severe acinar gland destruction
abnormal salivary gland duct morphology ( J:125129 )
• mild ductal changes in the salivary gland
large intestinal inflammation ( J:125129 )
• severe inflammation in the colon
decreased salivation ( J:125129 )
• mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function
salivary gland inflammation ( J:125129 )
• severe inflammation in the salivary glands
• major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils
• salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2
• although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small

endocrine/exocrine glands
abnormal salivary gland morphology ( J:125129 )
• severe acinar gland destruction
abnormal salivary gland duct morphology ( J:125129 )
• mild ductal changes in the salivary gland
decreased salivation ( J:125129 )
• mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function
salivary gland inflammation ( J:125129 )
• severe inflammation in the salivary glands
• major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils
• salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2
• although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small
lacrimal gland inflammation ( J:125129 )
• severe inflammation in the lacrimal glands

homeostasis/metabolism
decreased salivation ( J:125129 )
• mice exhibit an increase in the lag time and a reduction of saliva production when treated with Pilocarpine, indicating loss of salivary gland function

immune system
large intestinal inflammation ( J:125129 )
• severe inflammation in the colon
salivary gland inflammation ( J:125129 )
• severe inflammation in the salivary glands
• major population of infiltrating leukocytes are lymphocytes and to a much lower extent neutrophils
• salivary glands show presence of a large fraction of T cells and a ratio of CD4+/CD8+ of 1/2
• although more Gr-1+ cells are seen in the salivary glands, the absolute number of neutrophils is relatively small
lacrimal gland inflammation ( J:125129 )
• severe inflammation in the lacrimal glands
lung inflammation ( J:125129 )
• severe lung inflammation
• however, mice do not exhibit skin inflammation

respiratory system
lung inflammation ( J:125129 )
• severe lung inflammation
• however, mice do not exhibit skin inflammation

vision/eye
lacrimal gland inflammation ( J:125129 )
• severe inflammation in the lacrimal glands

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
Sjogren's syndrome DOID:12894 OMIM:270150
 J:125129




Genotype
MGI:2179512
hm3
Allelic
Composition		 Il2ratm1Dw/Il2ratm1Dw
Genetic
Background		 involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:64279 )
• about 25% die between 8 and 20 weeks of age with severe anemia

hematopoietic system
enlarged spleen ( J:64279 )
• develop enlargement of the spleen between 4 and 6 weeks of age
hemolytic anemia ( J:64279 )
• develops as mice age
increased lymphocyte cell number ( J:64279 )
• polyclonal expansion of lymphocytes beginning between 4 and 6 weeks of age
abnormal T cell morphology ( J:64279 )
• T cells are slightly larger than in wild-type in adults, however T and B cell development is normal up to 4 weeks after birth
increased memory T cell number ( J:64279 )
• increased numbers of cells bearing marker for memory cells
increased IgA level ( J:64279 )
• 5- to 30-fold increase in IgA
increased IgG level ( J:64279 )
• 5- to 30-fold increase in IgG1, IgG2a, and IgG2b
abnormal T cell physiology ( J:64279 )
• proliferative responses to CD3 are reduced in T cells
• peripheral deletion of staphylococcal enterotoxin B (SEB)-reactive T cells is impaired

immune system
colitis ( J:64279 )
• starting at 12-16 weeks of age, develop severe bowel inflammation that is confined to the colon
increased lymphocyte cell number ( J:64279 )
• polyclonal expansion of lymphocytes beginning between 4 and 6 weeks of age
abnormal T cell morphology ( J:64279 )
• T cells are slightly larger than in wild-type in adults, however T and B cell development is normal up to 4 weeks after birth
increased memory T cell number ( J:64279 )
• increased numbers of cells bearing marker for memory cells
increased IgA level ( J:64279 )
• 5- to 30-fold increase in IgA
increased IgG level ( J:64279 )
• 5- to 30-fold increase in IgG1, IgG2a, and IgG2b
abnormal T cell physiology ( J:64279 )
• proliferative responses to CD3 are reduced in T cells
• peripheral deletion of staphylococcal enterotoxin B (SEB)-reactive T cells is impaired
enlarged spleen ( J:64279 )
• develop enlargement of the spleen between 4 and 6 weeks of age
enlarged lymph nodes ( J:64279 )
• develop enlargement of the lymph nodes between 4 and 6 weeks of age

digestive/alimentary system
diarrhea ( J:64279 )
• starts at 12-16 weeks of age as mice develop inflammatory bowel disease
colitis ( J:64279 )
• starting at 12-16 weeks of age, develop severe bowel inflammation that is confined to the colon

growth/size/body
cachexia ( J:64279 )
• starts at 12-16 weeks of age as mice develop inflammatory bowel disease
enlarged spleen ( J:64279 )
• develop enlargement of the spleen between 4 and 6 weeks of age

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
inflammatory bowel disease DOID:0050589 OMIM:PS266600
 J:64279




Genotype
MGI:4356291
cx4
Allelic
Composition		 Faslpr/Faslpr
Il2ratm1Dw/Il2ratm1Dw
Tg(CD2-Ccdc86)1Hfuj/?
Genetic
Background		 B6.Cg-Il2ratm1Dw Faslpr Tg(CD2-Ccdc86)1Hfuj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Faslpr mutation (39 available); any Fas mutation (82 available)
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
Tg(CD2-Ccdc86)1Hfuj mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
enlarged spleen ( J:151722 )
• absence of the Fas receptor prevents the transgene from rescuing the splenomegaly associated with Il2ratm1Dw homozygotes

hematopoietic system
enlarged spleen ( J:151722 )
• absence of the Fas receptor prevents the transgene from rescuing the splenomegaly associated with Il2ratm1Dw homozygotes

growth/size/body
enlarged spleen ( J:151722 )
• absence of the Fas receptor prevents the transgene from rescuing the splenomegaly associated with Il2ratm1Dw homozygotes




Genotype
MGI:4356290
cx5
Allelic
Composition		 Il2ratm1Dw/Il2ratm1Dw
Tg(CD2-Ccdc86)1Hfuj/?
Genetic
Background		 B6.Cg-Il2ratm1Dw Tg(CD2-Ccdc86)1Hfuj
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
Tg(CD2-Ccdc86)1Hfuj mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:151722 )
N
• the presence of the transgene prevents the splenomegaly, the large number of T cells, and high circulating levels of IL-17 normally found in Il2ratm1Dw homozygotes
increased activated T cell number ( J:151722 )
• high expression of CD44 and CD69 suggests the transgene does not prevent the aberrant activation of peripheral T cells

hematopoietic system
increased activated T cell number ( J:151722 )
• high expression of CD44 and CD69 suggests the transgene does not prevent the aberrant activation of peripheral T cells




Genotype
MGI:3760115
cx6
Allelic
Composition		 Il2ratm1Dw/Il2ratm1Dw
Tg(Pgk1-HA)1.1Vbo/?
Tg(Tcra/Tcrb)1Vbo/?
Genetic
Background		 C.Cg-Il2ratm1Dw Tg(Pgk1-HA)1.1Vbo Tg(Tcra/Tcrb)1Vbo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
Tg(Pgk1-HA)1.1Vbo mutation (0 available)
Tg(Tcra/Tcrb)1Vbo mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased regulatory T cell number ( J:112603 )
• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 signaling is needed to maintain this regulatory T subset
increased regulatory T cell number ( J:112603 )
• the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to transgenic mice that have the Il2ra locus intact, indicating that IL2 signalling is not needed to generate this regulatory T cell subset
abnormal regulatory T cell physiology ( J:112603 )
• CD25-positive CD4 T cells from the thymus have a reduced capacity to suppress T cell proliferation in vitro

hematopoietic system
decreased regulatory T cell number ( J:112603 )
• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 signaling is needed to maintain this regulatory T subset
increased regulatory T cell number ( J:112603 )
• the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to transgenic mice that have the Il2ra locus intact, indicating that IL2 signalling is not needed to generate this regulatory T cell subset
abnormal regulatory T cell physiology ( J:112603 )
• CD25-positive CD4 T cells from the thymus have a reduced capacity to suppress T cell proliferation in vitro




Genotype
MGI:5141604
cx7
Allelic
Composition		 Cdk6tm2.1Phin/Cdk6tm2.1Phin
Il2ratm1Dw/Il2ratm1Dw
Genetic
Background		 involves: 129S4/SvJae * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdk6tm2.1Phin mutation (1 available); any Cdk6 mutation (39 available)
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:174697 )
N
• mice exhibit normal thymocyte cellularity and accumulation in DN3 stage compared to in Cdk6tm1.1Phin homozygotes
increased hematopoietic stem cell number ( J:174697 )
• compared with control and Cdk6tm2.1Phin homozygotes

immune system




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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory