Slc23a4^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc23a4^{tm1b(KOMP)Wtsi}
• Name: solute carrier family 23 member 4; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7859347
• Synonyms: Slc23a4 lacZ
• Gene: Slc23a4 Location: Chr6:34922213-34965339 bp, - strand Genetic Position: Chr6, 15.2 cM
• Alliance: Slc23a4^{tm1b(KOMP)Wtsi} page
• IMPC: Slc23a4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:361518
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34933523 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 34934457. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:361518)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc23a4 Mutation: 24 strains or lines available

References

• Original: J:361518 Terada K, et al., Pseudogenization of the Slc23a4 gene is necessary for the survival of Xdh-deficient mice. Sci Rep. 2025 Jan 25;15(1):3250
• All: 1 reference(s)