Gt(ROSA)26Sor^{tm4(tTA,tetO-TREM2*R47H,-EGFP)Gbu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(tTA,tetO-TREM2*R47H,-EGFP)Gbu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Guojun Bu
• MGI ID: MGI:7541481
• Synonyms: TREM2-R47H
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(tTA,tetO-TREM2*R47H,-EGFP)Gbu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341604
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct inserted a floxed neomycin stop cassette, tTA, insulator, hCMV*1 promoter with a tetracycline responsive element, intron, human TREM2 with the mutation encoding the amino acid substitution arginine with histidine at position 47 (R47H) plus tagged with 5' HA and 3' triple FLAG tags, IRES, and EGFP reporter gene. (J:341604)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1098 strains or lines available

References

• Original: J:341604 Zhao N, et al., Elevating microglia TREM2 reduces amyloid seeding and suppresses disease-associated microglia. J Exp Med. 2022 Dec 5;219(12)
• All: 2 reference(s)