Letmd1^em1Kshi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Letmd1^em1Kshi
• Name: LETM1 domain containing 1; endonuclease-mediated mutation 1, Shihuan Kuang
• MGI ID: MGI:7385074
• Gene: Letmd1 Location: Chr15:100366904-100377045 bp, + strand Genetic Position: Chr15, 56.31 cM
• Alliance: Letmd1^em1Kshi page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology in combination with mouse oviduct electroporation (i-GONAD; improved-Genome editing via Oviducal Nucleic Acids Delivery) deleted exons 3 to 7, causing a frameshift and inducing a stop codon, resulting in a truncated protein of 104 aa excluding the transmembrane domain a majority of the LETM1 domain. Western blot analysis confirmed the absence of protein. (J:318013)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Letmd1 Mutation: 17 strains or lines available

References

• Original: J:318013 Snyder MM, et al., LETMD1 is required for mitochondrial structure and thermogenic function of brown adipocytes. FASEB J. 2021 Nov;35(11):e21965
• All: 2 reference(s)